TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3198841.2.3
(792 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816396.1|CN816396 HRO4520_C05_F10ZS5 Lib AA071E1X Aven... 360 e-100
>gb|CN816396.1|CN816396 HRO4520_C05_F10ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4520_C05_F10, mRNA sequence
Length = 851
Score = 360 bits (780), Expect = e-100
Identities = 129/162 (79%), Positives = 142/162 (87%)
Frame = +3 / +2
Query: 306 INGSNPDDYEKFEKGIMHYGCPHYRRRCRIRAPCCNEIFDCRHCHNETKNSIKIDKMKRH 485
INGS+PDDYE+ EKG+M YGC HYRRRCRIRAPCCNEIFDCRHCHNE+KNSIK D ++RH
Sbjct: 29 INGSSPDDYERLEKGLMQYGCAHYRRRCRIRAPCCNEIFDCRHCHNESKNSIKTDVIRRH 208
Query: 486 ELPRHEVQQVVCSLCGTEQEVRQVCINCGVCMGKYFCGLCKLFDDDVSKQQYHCNXXXXX 665
EL RHEVQQV+CSLCGTEQEV ++CI+CGVCMGKYFC +CKLFDDD+SKQQYHC+
Sbjct: 209 ELSRHEVQQVICSLCGTEQEVGKICISCGVCMGKYFCEVCKLFDDDISKQQYHCHGCGIC 388
Query: 666 XXXXXENFFHCSKCGCCYSIALKNSHACVEGAMHHDCPICFE 791
ENFFHCSKCGCCYS LKNSHACVEGAMHHDCPICFE
Sbjct: 389 RIGGGENFFHCSKCGCCYSTVLKNSHACVEGAMHHDCPICFE 514
Score = 216 bits (466), Expect = 4e-057
Identities = 99/162 (61%), Positives = 117/162 (72%)
Frame = -2 / -2
Query: 791 LEADWTVMMHRPFNTCMTILQRN*IATSTF*TVEEILPPSDSTYSTSVAVILLFRDIIVE 612
LEADWTVMMH FNTCM +LQ I TSTF* V+EIL ++STYST++ VILLF DII++
Sbjct: 514 LEADWTVMMHCSFNTCMAVLQHCRITTSTF*AVKEILAATNSTYSTTMTVILLF*DIIIK 335
Query: 611 EFAQTTEVFPHANTAIDTYLSHLLFSATQ*AYNLLHFMAWKFMPLHFINFNGILSFIVAV 432
EFA TE+ H T D Y +LLFS T *AY+LLHFM KFMP + I+FN IL FIVAV
Sbjct: 334 EFAHFTEILSHTYTTADAYFPYLLFSTT**AYDLLHFMT*KFMPPNHISFNRIL*FIVAV 155
Query: 431 SAIKNFIAARSSYAASPSIMWTPIVHYPFLKFLIVIRV*TID 306
IKN +AA SYAA PS+M TPI+H L+ LI+IR ID
Sbjct: 154 PTIKNLVAAWCSYAAPPSVMCTPILHQSLLQPLIIIRARAID 29
Score = 208 bits (449), Expect = 8e-055
Identities = 98/163 (60%), Positives = 112/163 (68%)
Frame = -1 / -1
Query: 792 TRSRLDSHDASPLQHMHDYSSAQLNSNIHILNSGRNSPSLRFYIFHIRCSDTAV*RHHRR 613
TRSRLDSHDA LQHMH SS N+NIHIL+S RNS +FYIFH SDTAV R+H +
Sbjct: 515 TRSRLDSHDALLLQHMHGCSSTL*NNNIHILSSERNSRRHQFYIFHNHDSDTAVLRYHHQ 336
Query: 612 RVCTNHRSISPCKHRN*YILVAPPVQCHTMSIQPAALHGVEVHASSFYQF*WNS*FHCGS 433
RVCT HR+ P H +* I P VQ H MSI PAALH V+VHA+ +QF* NS HCGS
Sbjct: 335 RVCTLHRNTFPYIHHS*CIFSLPLVQYHIMSI*PAALHDVKVHAA*SHQF*SNSLIHCGS 156
Query: 432 VGNQKFHCSKELLCGISFYNVDTHSALSLSQISHSHQGLNH*S 304
NQK CS L+CG SF NV TH+A S SH+HQG +H*S
Sbjct: 155 ADNQKSRCSMVLVCGTSFGNVHTHTASIPSPASHNHQG*SH*S 27
Score = 204 bits (440), Expect = 1e-053
Identities = 100/162 (61%), Positives = 109/162 (67%)
Frame = +1 / +3
Query: 307 SMVQTLMTMRNLRKG*CTMGVHIIEGDAA*ELLAAMKFLIADTATMKLRIPLKLIK*RGM 486
SM LM MR R+ *C+MGVHI EG AA E AA +FLI TATM RI LKL+ GM
Sbjct: 30 SMALALMIMRGWRRD*CSMGVHITEGGAAYEHHAATRFLIVGTATMNQRIRLKLM*LGGM 209
Query: 487 NFHAMKCSRLYAHCVALNRRCDKYVSIAVFAWGNTSVVCANSSTMMSLNSSITATDVEYV 666
NFH MKCSR YAH V LN+R KY S V+ W + SV CANS M+S NSSIT VEYV
Sbjct: 210 NFHVMKCSRSYAHYVVLNKR*GKYASAVVYVWESISVKCANSLMMISQNSSITVMVVEYV 389
Query: 667 ESEGGRISSTVQNVDVAIQLR*RIVMHVLKGRCIMTVQSASS 792
E RIS T QNVDV I *R MHVLK +CIMTVQSASS
Sbjct: 390 ELVAARISFTAQNVDVVILQC*RTAMHVLKEQCIMTVQSASS 515
Score = 204 bits (439), Expect(2) = 3e-070
Identities = 85/114 (74%), Positives = 90/114 (78%)
Frame = -3 / -3
Query: 646 Q*YCCLETSSSKSLHKPQKYFPMQTPQLIHTCRTSCSVPHNEHTTCCTSWRGSSCLFILS 467
Q*YCC E SSSKSLH QKYFP+ TPQL+H TSCSVPHNEH TCCTS R SSC I S
Sbjct: 369 Q*YCCFEISSSKSLHTSQKYFPIHTPQLMHIFPTSCSVPHNEHMTCCTS*RESSCRLITS 190
Query: 466 ILMEFLVSLWQCRQSKISLQQGALMRHLLL*CGHP*CIIPFSNFS*SSGFEPLI 305
+L+EF SLWQCRQSKISLQ GA MRHLL *C HP CI PFS+ S*SSG EPLI
Sbjct: 189 VLIEFFDSLWQCRQSKISLQHGARMRHLLR*CAHPYCINPFSSLS*SSGLEPLI 28
Score = 185 bits (399), Expect = 7e-048
Identities = 99/162 (61%), Positives = 116/162 (71%)
Frame = +2 / +1
Query: 305 DQWFKP**L*EI*ERDNALWVSTL*KEMPHKSSLLQ*NF*LPTLPQ*N*EFH*N**NEEA 484
DQW P**L*E E +A+WV TL KE+PH S++LQ +F*L LPQ* EF *N* + A
Sbjct: 28 DQWL*P**L*EAGEGIDAVWVCTLPKEVPHTSTMLQRDF*LSALPQ*IKEFD*N*CD*AA 207
Query: 485 *TSTP*SAAGCMLIVWH*TGGATSMYQLRCLHGEILLWFVQTLRR*CL*TAVSLQRMWNM 664
*T T *SAAG MLI+W+*T G +M+QL C++G++ L VQTL * L TAVSL +WNM
Sbjct: 208 *TFTS*SAAGHMLIMWY*TRGRENMHQLWCMYGKVFL*SVQTL***YLKTAVSLSWLWNM 387
Query: 665 *NRREGEFLPLFKMWMLLFNCAEE*SCMC*RGDAS*LSNLLR 790
*N EFL L KMWMLLF EE CMC*R +AS*LSNLLR
Sbjct: 388 *NWWRREFLSLLKMWMLLFYSVEEQPCMC*RSNAS*LSNLLR 513
Score = 76.7 bits (161), Expect(2) = 3e-070
Identities = 32/37 (86%), Positives = 34/37 (91%)
Frame = -3 / -3
Query: 790 SKQIGQS*CIAPSTHA*LFFSAIE*QHPHFEQWKKFS 680
SKQIGQS*CIAPSTHA LFF+ +E*QHPHFEQ KKFS
Sbjct: 513 SKQIGQS*CIAPSTHAWLFFNTVE*QHPHFEQ*KKFS 403
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 11,174,522
Number of Sequences: 8143
Number of extensions: 186547
Number of successful extensions: 1491
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 267814360
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)