TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3106156.2.1
(1016 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN819880.1|CN819880 HRO4407_C04_F07ZS5 Lib AA069E1X Aven... 351 e-111
gb|CN814897.1|CN814897 HRO4503_F11_L20ZS5 Lib AA071E1X Aven... 175 1e-046
>gb|CN819880.1|CN819880 HRO4407_C04_F07ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4407_C04_F07, mRNA
sequence
Length = 741
Score = 351 bits (761), Expect(2) = e-111
Identities = 139/165 (84%), Positives = 149/165 (90%)
Frame = -1 / +1
Query: 773 RYFNVIGSDPEGRLGEAPRPELREHGRISGACFDAALGVIPGLKVKGTDYPTADGTCIRD 594
RYFNVIGSDPEG LGEAPRPELREHGRISGACFDAA G IPGLKV+GTDYPTADGTCIRD
Sbjct: 145 RYFNVIGSDPEGLLGEAPRPELREHGRISGACFDAASGTIPGLKVRGTDYPTADGTCIRD 324
Query: 593 YIDVTDLVDAHVKALNKAEPRKVGIYNVGTGRGRSVNEFVDACKKATGVNIKIEYLNRRP 414
YIDVTDLVDAHVKAL KAEP KVGIYNVGTG+GRSV EFV+ACK ATG IK+EYL RRP
Sbjct: 325 YIDVTDLVDAHVKALGKAEPSKVGIYNVGTGQGRSVKEFVEACKAATGATIKVEYLTRRP 504
Query: 413 GDYAEVYSDPTKINKELNWTAHYTDLKESLSVAWRWQKSHPRGYG 279
GDYAEVYS+P+KI ELNWTA TDL+ESL+ AW+WQK+HP GYG
Sbjct: 505 GDYAEVYSNPSKIRDELNWTAQRTDLRESLATAWKWQKTHPGGYG 639
Score = 258 bits (557), Expect(2) = 4e-080
Identities = 121/165 (73%), Positives = 132/165 (80%)
Frame = +1 / -2
Query: 280 P*PRGCDFCHLHATDRLSLRSV**AVQLSSLFIFVGSLYTSA*SPGLLLRYSILMLTPVA 459
P P GC FCH HA RLS RSV AVQ SS I G L TSA*SPGL +RYS LM+ PVA
Sbjct: 638 PYPPGCVFCHFHAVARLSRRSVRWAVQFSSSRILDGLL*TSA*SPGLRVRYSTLMVAPVA 459
Query: 460 FLQASTNSLTERPLPVPTL*MPTFLGSALLSAFT*ASTKSVTSI*SLMQVPSAVG*SVPL 639
L ASTNS T+ P PVPTL*+PT LGSAL A T*AST+SVTS+*SL+QVPSAVG*SVP
Sbjct: 458 ALHASTNSFTDLPCPVPTL*IPTLLGSALPRALT*ASTRSVTSM*SLIQVPSAVG*SVPR 279
Query: 640 TFNPGMTPNAASKQAPDIRPCSRSSGLGASPNLPSGSDPITLKYL 774
TF+PGM P+AASKQAP+I PCSR+SGLGASP PSGSDPITLKYL
Sbjct: 278 TFSPGMVPDAASKQAPEILPCSRNSGLGASPRSPSGSDPITLKYL 144
Score = 150 bits (322), Expect(3) = 6e-046
Identities = 73/167 (43%), Positives = 101/167 (60%)
Frame = -3 / +3
Query: 771 IFQCYWI*P*GKIR*GS*ARTSRAWSDIWGLL*CSIRSHSRIKG*RNRLSHS*WNLHKRL 592
+ QC WI P G R S AR +RA D W L CS+R+HSR +G RN L + *WNL+ L
Sbjct: 147 VLQCDWIRPGGTPRGSSKARVARARKDFWCLFRCSVRNHSRAEGSRNGLPYC*WNLY*GL 326
Query: 591 Y*CHRFS*CSCESTQQGRA*KSRHLQCWNRKRSLS**VC*CLQEGNWGQHQN*VPQQKAR 412
+ CHR CSC+S+ A*+S +LQCW+ R +S VC +Q G+ HQ +P + R
Sbjct: 327 HRCHRSRRCSCQSSW*S*A*QSWNLQCWHGAR*VSKGVCRSVQGGDRSYHQGRIPYPETR 506
Query: 411 RLCRSIQRPNKDKQGAQLDSSLYRSQREPISRMEVAKVASAWLRAKL 271
RLC S+Q+P +D + A+LDS + S R+P ME+A+ +R L
Sbjct: 507 RLC*SLQQPVQDPRRAELDSPTHGSSRKPRDGMEMAEDTPRGIRIGL 647
Score = 103 bits (219), Expect(2) = 4e-031
Identities = 57/157 (36%), Positives = 80/157 (50%)
Frame = +3 / -1
Query: 300 LLPPPCD**ALFEIGIMSCPVELLVYLCWVAVYFCIISWPSVEVLNFDVDPSCLLAGINK 479
LLP PC A +I + CPV+L+ L VAV F I+SW +V + D LA K
Sbjct: 618 LLPFPCRREAFAKIRALGCPVQLVADLGRVAVNFSIVSWSPGKVFDLDGSSCRRLARFYK 439
Query: 480 LIN*ATSSCSNIVNADFSRLCLVECFHMSXXXXXXXXXXSYAGSISCGIICSFNL*SWND 659
L+ * T +NIV+++F+RL + F MS SIS ++ S NL WN
Sbjct: 438 LLY*PTLPRANIVDSNFARLSFTKSFDMSIDEICDIYVVPNTSSISSRVVRSSNLQPWNG 259
Query: 660 S*CCIKASPRYPTMLSKFWPXXXXXXXXXXXXNNIEI 770
S* CI+ S R P++L++ WP N+IE+
Sbjct: 258 S*RCIETSTRNPSVLAQLWPWSFPEESLRV*SNHIEV 148
Score = 65.7 bits (137), Expect(2) = e-111
Identities = 25/40 (62%), Positives = 32/40 (80%)
Frame = -1 / +1
Query: 926 MPITEATP*FPINPYGKAKKMAEDIIIDFSNSKGVDMAVM 807
MPITE TP PINPYGKAKKM+EDII+DFS + + + ++
Sbjct: 25 MPITETTPQVPINPYGKAKKMSEDIILDFSKNSNMAVMIL 144
Score = 56.1 bits (116), Expect(2) = 4e-080
Identities = 25/31 (80%), Positives = 26/31 (83%)
Frame = +1 / -2
Query: 835 FEKSIMISSAIFLAFPYGLIGN*GVASVIGI 927
FEKS M+SS IF AFPYGLIG *GV SVIGI
Sbjct: 116 FEKSRMMSSDIFFAFPYGLIGT*GVVSVIGI 24
Score = 46.9 bits (96), Expect(2) = 4e-031
Identities = 18/31 (58%), Positives = 22/31 (70%)
Frame = +3 / -1
Query: 834 V*EIYYDILCHFLSFSIWIDRELRCGFCNRH 926
+*+I D+L H F IWIDR LRC FC+RH
Sbjct: 117 L*KI*NDVL*HLFCFPIWIDRNLRCCFCDRH 25
Score = 43.7 bits (89), Expect(3) = 6e-046
Identities = 16/31 (51%), Positives = 25/31 (80%)
Frame = -3 / +3
Query: 927 DAYYRSHTLVPYQSIWKS*ENGRGYHNRFLK 835
+AY+R++T YQSIW+S ++ RG+H+RF K
Sbjct: 24 NAYHRNNTSGSYQSIWESKKDVRGHHSRFFK 116
Score = 24.0 bits (46), Expect(3) = 6e-046
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = -3 / +3
Query: 819 HGCHDFKV 796
HGCHD KV
Sbjct: 126 HGCHDLKV 149
>gb|CN814897.1|CN814897 HRO4503_F11_L20ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4503_F11_L20, mRNA sequence
Length = 610
Score = 175 bits (376), Expect(2) = 1e-046
Identities = 69/79 (87%), Positives = 75/79 (94%)
Frame = -1 / +1
Query: 497 GRSVNEFVDACKKATGVNIKIEYLNRRPGDYAEVYSDPTKINKELNWTAHYTDLKESLSV 318
GRSV EFVDACKKATGV+IK+EYL+RRPGDYAEV+SDPTKIN ELNWTA +TDLKESLSV
Sbjct: 70 GRSVKEFVDACKKATGVDIKVEYLSRRPGDYAEVFSDPTKINSELNWTAQHTDLKESLSV 249
Query: 317 AWRWQKSHPRGYGPN*I*P 261
AWRWQKSHPRGYG +*I*P
Sbjct: 250 AWRWQKSHPRGYGTD*I*P 306
Score = 156 bits (336), Expect(2) = 1e-039
Identities = 72/81 (88%), Positives = 73/81 (90%)
Frame = +1 / -3
Query: 256 SAGYI*FGP*PRGCDFCHLHATDRLSLRSV**AVQLSSLFIFVGSLYTSA*SPGLLLRYS 435
+ GYI* P*PRGCDFCHLHATDRLSLRSV *AVQLSSL IFVGSL TSA*SPGLLLRYS
Sbjct: 311 AVGYI*SVP*PRGCDFCHLHATDRLSLRSVC*AVQLSSLLIFVGSLNTSA*SPGLLLRYS 132
Query: 436 ILMLTPVAFLQASTNSLTERP 498
LM TPVAFLQASTNSLTERP
Sbjct: 131 TLMSTPVAFLQASTNSLTERP 69
Score = 112 bits (239), Expect = 1e-025
Identities = 50/78 (64%), Positives = 57/78 (73%)
Frame = +3 / -2
Query: 264 LYLVWPVATRMRLLPPPCD**ALFEIGIMSCPVELLVYLCWVAVYFCIISWPSVEVLNFD 443
LYLV PVA MRLLP P D AL EIG++S PVEL V LC V YF I+SWP EVLNFD
Sbjct: 303 LYLVCPVAPWMRLLPSPRDGQALLEIGMLSSPVELTVDLCRVTEYFSIVSWPPAEVLNFD 124
Query: 444 VDPSCLLAGINKLIN*AT 497
V+P CL AGI+KL++ T
Sbjct: 123 VNPGCLFAGIHKLLDGTT 70
Score = 110 bits (234), Expect(2) = 2e-026
Identities = 50/73 (68%), Positives = 59/73 (80%)
Frame = -3 / +3
Query: 480 VC*CLQEGNWGQHQN*VPQQKARRLCRSIQRPNKDKQGAQLDSSLYRSQREPISRMEVAK 301
VC CLQ+GN G HQ+*VPQQ+ARRLC SIQ P KD+Q AQLD S YRSQ EP+ R+E+A+
Sbjct: 87 VCGCLQKGNRG*HQS*VPQQEARRLC*SIQ*PYKDQQ*AQLDCSTYRSQGEPVRRVEMAE 266
Query: 300 VASAWLRAKLNIA 262
VAS LR +LNIA
Sbjct: 267 VASTGLRDRLNIA 305
Score = 100 bits (212), Expect = 5e-022
Identities = 52/79 (65%), Positives = 56/79 (70%)
Frame = +2 / -1
Query: 263 AIFSLARSHADATFATSMRLIGSL*DRYNELSS*APCLSLLGRCILLHNLLAFC*GTQF* 442
AIFSL+RS DAT A S R GS *DRY E SS*A C SL G IL H+LLA C*GTQ *
Sbjct: 304 AIFSLSRSPVDATSAISTRRTGSP*DRYVEQSS*AHC*SL*GH*ILQHSLLASC*GTQL* 125
Query: 443 C*PQLPSCRHQQTH*LSDL 499
C P+LP CRH QT * +DL
Sbjct: 124 CQPRLPFCRHPQTP*RNDL 68
Score = 27.2 bits (53), Expect(2) = 1e-046
Identities = 13/20 (65%), Positives = 15/20 (75%)
Frame = -3 / +3
Query: 234 NTY*FFS*RYKILLRYSSKS 175
NTY*F S* Y+IL+ YS S
Sbjct: 336 NTY*FCS*WYRILVLYSLPS 395
Score = 24.4 bits (47), Expect(2) = 2e-026
Identities = 11/17 (64%), Positives = 14/17 (82%)
Frame = -2 / +2
Query: 235 QYILVL*LEIQDLAAIL 185
+YIL+L*L + DL AIL
Sbjct: 335 KYILIL*LVV*DLGAIL 385
Score = 22.1 bits (42), Expect(2) = 1e-039
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = +3 / -2
Query: 186 SIAARSCISN*RTNMY 233
SIA RS +N R NMY
Sbjct: 384 SIAPRSYTTNYRINMY 337
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 10,771,627
Number of Sequences: 8143
Number of extensions: 145941
Number of successful extensions: 731
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 355503330
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)