TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3071761.2.1
(638 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN817366.1|CN817366 HRO4465_D03_H05ZS5 Lib AA070E1X Aven... 224 1e-059
gb|CN816052.1|CN816052 HRO4516_D03_H06ZS5 Lib AA071E1X Aven... 148 8e-037
>gb|CN817366.1|CN817366 HRO4465_D03_H05ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4465_D03_H05, mRNA sequence
Length = 633
Score = 224 bits (484), Expect = 1e-059
Identities = 93/111 (83%), Positives = 105/111 (94%)
Frame = +2 / +3
Query: 2 FIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMNDDLAGEIPVAFIVRTEGSQ 181
FIVDRLKEIIKYKGFQV PAELEALLITHP+IKDAAVV + DDL GE+PVAFI R EGS+
Sbjct: 42 FIVDRLKEIIKYKGFQVAPAELEALLITHPDIKDAAVVGVKDDLCGEVPVAFIKRIEGSE 221
Query: 182 VTEDEIKQFVAKEVVFYKKIHKVFFTESIPKNPSGKILRKDLRARLAAGVH 334
+TE+EIKQFV+KEVVFYK+I+KV+FT+SIPKNPSGKILRKDLRARLAAG+H
Sbjct: 222 ITENEIKQFVSKEVVFYKRINKVYFTDSIPKNPSGKILRKDLRARLAAGIH 374
Score = 197 bits (424), Expect = 2e-051
Identities = 90/109 (82%), Positives = 95/109 (87%)
Frame = -3 / -3
Query: 327 PAASLALKSFLRILPDGFLGMDSVKKTLWIFL*KTTSLATNCLISSSVT*EPSVRTMKAT 148
PAASLALKSFLRILP+GFLGM+SVK TL I L*KTTSL TNCLIS SV +PS+R MKAT
Sbjct: 367 PAASLALKSFLRILPEGFLGMESVK*TLLILL*KTTSLETNCLISFSVISDPSIRLMKAT 188
Query: 147 GISPARSSFIDTTAASLISGCVMRSASSSAGGTWKPLYLMISLSLSTMK 1
G SP RSSF TTAASL+SGCVMR ASSSAG TWKPLYLMISLSLSTMK
Sbjct: 187 GTSPQRSSFTPTTAASLMSGCVMRRASSSAGATWKPLYLMISLSLSTMK 41
Score = 118 bits (253), Expect(2) = 8e-034
Identities = 54/77 (70%), Positives = 59/77 (76%)
Frame = +1 / +2
Query: 97 QGRRRRINER*PCW*NPGRLHRADRRFSSHRG*DQAIRRQGGGFLQEDPQGLLHRIHPQE 276
QGRRRR +E * + GRLH+ADRR HR DQAIR QGG FLQED QGLLHR+H QE
Sbjct: 137 QGRRRRRSEG*SLRRSAGRLHQADRRV*DHRERDQAIRLQGGCFLQEDQQGLLHRLHSQE 316
Query: 277 PVGQDPEEGLESQARRR 327
P QDP+EGLESQARRR
Sbjct: 317 PFRQDPKEGLESQARRR 367
Score = 100 bits (213), Expect(2) = 1e-024
Identities = 46/78 (58%), Positives = 53/78 (67%)
Frame = -2 / -2
Query: 334 VNTGGEPGSQVLPQDLARRVLGDGFGEEDLVDLLVENHXXXXXXXXXXXXXLRTFGPHDE 155
V+ GGEPGSQVLP DLA RVLG+G GE DLVD LVEN+ LR F P DE
Sbjct: 374 VDAGGEPGSQVLP*DLAGRVLGNGVGEVDLVDPLVENNLLGDELLDLVLGDLRPFDPLDE 195
Query: 154 GDRDFTSKVIVH*YDGGV 101
GDR F +++I+H YDGGV
Sbjct: 194 GDRHFAAEIILHSYDGGV 141
Score = 39.6 bits (80), Expect(2) = 8e-034
Identities = 15/15 (100%), Positives = 15/15 (100%)
Frame = +1 / +2
Query: 1 LHRRQAQGDHQVQGL 45
LHRRQAQGDHQVQGL
Sbjct: 41 LHRRQAQGDHQVQGL 85
Score = 26.7 bits (52), Expect(2) = 1e-024
Identities = 12/12 (100%), Positives = 12/12 (100%)
Frame = -1 / -1
Query: 38 CT**SP*ACRR* 3
CT**SP*ACRR*
Sbjct: 78 CT**SP*ACRR* 43
>gb|CN816052.1|CN816052 HRO4516_D03_H06ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4516_D03_H06, mRNA sequence
Length = 547
Score = 148 bits (318), Expect = 8e-037
Identities = 61/77 (79%), Positives = 72/77 (93%)
Frame = +2 / +2
Query: 104 AAVVSMNDDLAGEIPVAFIVRTEGSQVTEDEIKQFVAKEVVFYKKIHKVFFTESIPKNPS 283
AAVV + DDL GE+PVAFI R EGS++TE+EIKQFV+KEVVFYK+I+KV+FT+SIPKNPS
Sbjct: 11 AAVV*VKDDLCGEVPVAFIKRIEGSEITENEIKQFVSKEVVFYKRINKVYFTDSIPKNPS 190
Query: 284 GKILRKDLRARLAAGVH 334
GKILRKDLRARLAAG+H
Sbjct: 191 GKILRKDLRARLAAGIH 241
Score = 126 bits (270), Expect = 3e-030
Identities = 59/75 (78%), Positives = 63/75 (84%)
Frame = -3 / -2
Query: 327 PAASLALKSFLRILPDGFLGMDSVKKTLWIFL*KTTSLATNCLISSSVT*EPSVRTMKAT 148
PAASLALKSFLRILP+GFLGM+SVK TL I L*KTTSL TNCLIS SV +PS+R MKAT
Sbjct: 234 PAASLALKSFLRILPEGFLGMESVK*TLLILL*KTTSLETNCLISFSVISDPSIRLMKAT 55
Query: 147 GISPARSSFIDTTAA 103
G SP RSSF TTAA
Sbjct: 54 GTSPQRSSFTHTTAA 10
Score = 117 bits (250), Expect = 2e-027
Identities = 52/74 (70%), Positives = 58/74 (78%)
Frame = +1 / +1
Query: 106 RRRINER*PCW*NPGRLHRADRRFSSHRG*DQAIRRQGGGFLQEDPQGLLHRIHPQEPVG 285
RRR++E * +PGRLH+ADRR HR DQAIR QGG FLQED QGLLHR+H QEP
Sbjct: 13 RRRMSEG*SLRRSPGRLHQADRRI*DHRERDQAIRLQGGCFLQEDQQGLLHRLHSQEPFR 192
Query: 286 QDPEEGLESQARRR 327
QDP+EGLESQARRR
Sbjct: 193 QDPKEGLESQARRR 234
Score = 102 bits (218), Expect = 5e-023
Identities = 46/77 (59%), Positives = 54/77 (70%)
Frame = -2 / -1
Query: 334 VNTGGEPGSQVLPQDLARRVLGDGFGEEDLVDLLVENHXXXXXXXXXXXXXLRTFGPHDE 155
V+ GGEPGSQVLP DLA RVLG+G GE DLVD LVEN+ LR+F P DE
Sbjct: 241 VDAGGEPGSQVLP*DLAGRVLGNGVGEVDLVDPLVENNLLGDELLDLVLGDLRSFDPLDE 62
Query: 154 GDRDFTSKVIVH*YDGG 104
GDRDF +++I+H YDGG
Sbjct: 61 GDRDFAAEIILHSYDGG 11
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,655,057
Number of Sequences: 8143
Number of extensions: 97307
Number of successful extensions: 2377
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 208331770
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)