TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3067375.2.1
(1829 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816530.1|CN816530 HRO4521_G10_M19ZS5 Lib AA071E1X Aven... 376 e-109
>gb|CN816530.1|CN816530 HRO4521_G10_M19ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4521_G10_M19, mRNA sequence
Length = 749
Score = 376 bits (815), Expect(2) = e-109
Identities = 155/225 (68%), Positives = 185/225 (82%)
Frame = -3 / +1
Query: 1506 KEDHKVPVKCRKHISIIAVDSVKKEDLIQIIRNSVEATRTGTMSGSTGFVLSTSLTIAEL 1327
+E P+ RKHI +I+VDSV KEDL++IIRN++EA T STGFVLSTSLTI+E+
Sbjct: 73 EERSNAPIS*RKHIIVISVDSVNKEDLVRIIRNAIEAAHTENTPASTGFVLSTSLTISEI 252
Query: 1326 QSVIVRTGMLPTDFDAFICNSGSDIYYPLQSSDVPSNSRVTFALDHNYRSHIEYRWGGEG 1147
S++V GM P FDAFICNSGS IYYP S + P++S+VT + N++SHIEYRWGGEG
Sbjct: 253 CSLLVSVGMHPAGFDAFICNSGSSIYYPSYSGNTPTSSKVTHVIHQNHQSHIEYRWGGEG 432
Query: 1146 LRKYLVKWASSVVERRGRTEKQVIFEDSEHSSTYCLAFRVVNPNHLPPLKELQKLMRIQS 967
LRKYLVKWA+SVVER+GR E+Q+IFEDSEHSS Y AF+V NPNHLPPLK+L+KLMRIQS
Sbjct: 433 LRKYLVKWATSVVERKGRIERQMIFEDSEHSSAY*HAFKVDNPNHLPPLKDLRKLMRIQS 612
Query: 966 LRCHALYNHGATRLSVIPMHASRSQALRYLSIRWGIELPNAVVIV 832
LRC+ALYNH ATRLSV +HASRSQA+RYL IRWGIELPN VV+V
Sbjct: 613 LRCNALYNHSATRLSVTTIHASRSQAIRYLFIRWGIELPNIVVLV 747
Score = 291 bits (629), Expect(2) = 2e-082
Identities = 138/221 (62%), Positives = 159/221 (71%)
Frame = +2 / -1
Query: 833 TMTTAFGNSMPQRIDKYLRA*DRDACIGITDSLVAPWLYRA*QRSDWILINFCSSFKGGK 1012
T TT FGNS+PQRI+KYL A*+RDA I +TDSLVA WL A QRSDWILINF SF+GG+
Sbjct: 746 TRTTIFGNSIPQRINKYLIA*ERDA*IVVTDSLVALWL*SALQRSDWILINFLKSFRGGR 567
Query: 1013 *FGLTTLNARQYVEECSESSKMTCFSVLPLLSTTEEAHLTKYFLKPSPPQRYSICDR*LW 1192
*FGL+TLNA Q EECSESS + C S+LP LSTTE AH T+YFL+PSPPQRYSICD *
Sbjct: 566 *FGLSTLNACQ*AEECSESSNIICLSILPFLSTTEVAHFTRYFLRPSPPQRYSICDW*F* 387
Query: 1193 SNANVTRELLGTSLDCNG*YISLPLLHIKASKSVGSMPVRTITDCSSAIVSEVDSTKPVE 1372
VT EL+G + G* + LPLL +KASK G MP T ++ S IVS+VDSTKPVE
Sbjct: 386 CITCVTLELVGVLPEYEG**MLLPLLQMKASKPAGCMPTDTSSEQISDIVSDVDSTKPVE 207
Query: 1373 PDIVPVRVASTEFLII*IKSSFLTESTAMIDICFLHFTGTL 1495
+ V AS FLII +SS LTEST + ICFLH G L
Sbjct: 206 AGVFSV*AASIAFLIIRTRSSLLTESTEITIICFLHDIGAL 84
Score = 200 bits (431), Expect(2) = 3e-056
Identities = 103/217 (47%), Positives = 140/217 (64%)
Frame = -2 / +3
Query: 1480 VQEAYIYHCCRLC*KGRFNSNYQKLC*GYTHWNYVRFYRFCAVYFTDNSRATVCNSTHRH 1301
+++AY + C LC*+GR + + QK *G ++ + F F AV T+N R S RH
Sbjct: 99 MKKAYNCYFCGLC*QGRSSPDNQKCY*GCSYREHAGFNWFRAVNVTNNIRDLFTASICRH 278
Query: 1300 APD*L*CLYMQ*WERYILSITV**CAKQFPCYICVGP*LPITY*VSLGRRRFKEVLS*VG 1121
A * ++Q*WE ++LS * A QF Y C PITY*VSLGRRR K+V S +G
Sbjct: 279 ASCWF*RFHLQ*WE*HLLSFIFR*YAHQFQGYTCNTSKSPITY*VSLGRRRSKKVSSEMG 458
Query: 1120 FFSSRKKGEN*KASHL*RFGTLFNILSCI*SG*SKSFASFEGAAKVDENPVTALSCSV*P 941
+FS RKKG+N*KA+ +*RF T F +L+CI*SG SKS S + +VDE+PV L C++*P
Sbjct: 459 YFSGRKKGKN*KANDI*RF*TFFCLLTCI*SGQSKSSTSSKRLEEVDEDPVAPL*CAL*P 638
Query: 940 WRHQAICNSNACIAISGSKVFVYSLGHRVAKCCGHCW 830
+Q +CN + +++SG+KV VY+LG+RVAK C W
Sbjct: 639 QCYQTVCNYYSRVSLSGNKVLVYTLGNRVAKYCSPRW 749
Score = 84.5 bits (178), Expect(4) = 3e-041
Identities = 45/86 (52%), Positives = 51/86 (59%)
Frame = +3 / -2
Query: 849 LATLCPSE*TNTLEPEIAMHALELQIAWWRHGYTEHDNAVTGFSSTFAAPSKEANDLD*P 1028
LATL PS *T+TL PE LQ W GY H N TG SST ++ +E +DLD P
Sbjct: 730 LATLFPSV*TSTLLPESETRE**LQTVW*HCGYKAHYNGATGSSSTSSSLLEEVDDLDCP 551
Query: 1029 L*MQDNMLKSVPNLQR*LAFQFSPFF 1106
L*M N K+V NLQ AFQF PFF
Sbjct: 550 L*MHVNRQKNVQNLQISFAFQFFPFF 473
Score = 72.5 bits (152), Expect(4) = 1e-030
Identities = 44/81 (54%), Positives = 50/81 (61%)
Frame = -1 / +2
Query: 1073 LKIRNTLQHIVLHLEWLIQIICLL*RSCKS**ESSHCVVMLCITMAPPGYL*FQCMHRDL 894
LKI N L I +HL+W IQII LL*++ S** SS VVM IT P L* L
Sbjct: 506 LKILNILLPIDMHLKWTIQIIYLL*KT*GS**GSSRSVVMRFITTVLPDCL*LLFTRLAL 685
Query: 893 RL*GICLFAGA*SCQMLWSLL 831
R *G CL+AG *SCQ+L S L
Sbjct: 686 RQ*GTCLYAGE*SCQIL*SSL 748
Score = 63.4 bits (132), Expect(4) = 3e-041
Identities = 31/58 (53%), Positives = 39/58 (67%)
Frame = +1 / -3
Query: 1129 N*VLP*TFSSPTILNM*SVVMVQRKCNTGIAWHITRL*WIIYIAPTIAYKGIKVSREH 1302
+*+L *TFSSPTILNM* V+++ CN G IT +* II PTIA + +K SR H
Sbjct: 450 H*ILS*TFSSPTILNM*LVILMYYMCNLGTGGRIT*I*RIINATPTIADESVKTSRMH 277
Score = 61.6 bits (128), Expect(5) = 2e-027
Identities = 24/50 (48%), Positives = 37/50 (74%)
Frame = +1 / -3
Query: 832 NNDHSIWQLYAPANRQIP*SLRSRCMHWNYR*PGGAMVIQSMTTQ*LDSH 981
N D++IWQLY+PA +Q+P LR+R ++ +YR G +VI+ +TT+ LD H
Sbjct: 747 NEDYNIWQLYSPAYKQVPYCLRARRVNSSYRQSGSTVVIKRITTERLDPH 598
Score = 53.8 bits (111), Expect(4) = 1e-030
Identities = 30/60 (50%), Positives = 37/60 (61%)
Frame = -1 / +2
Query: 1316 *YAQACSRLTLMPLYAIVGAIYIIHYSLVMCQAIPVLHLRWTITTDHILSIVGEEKV*GS 1137
*Y AC L L AIVG +II + V+ +P LH+ + T+HILSIVGEEKV* S
Sbjct: 263 *YL*ACILLVLTLSSAIVGVAFIILHIQVIRPPVPRLHM*YIKITNHILSIVGEEKV*ES 442
Score = 41.8 bits (85), Expect(4) = 3e-041
Identities = 21/50 (42%), Positives = 30/50 (60%)
Frame = +1 / -3
Query: 1354 QHKTCRT*HSSSACSLNRVSDNLN*IFLFNRVYSNDRYMLPALHRHLVVF 1503
QH+T + SLN +SD +*IFL NRV+ N+ YML + +R + F
Sbjct: 225 QHETS*SRRVLCMSSLNSISDYPD*IFLVNRVHRNNNYMLSS*YRCITPF 76
Score = 41.4 bits (84), Expect(5) = 2e-027
Identities = 19/43 (44%), Positives = 26/43 (60%)
Frame = +3 / -2
Query: 1164 DTQYVIGSYGPTQM*HGNCLAHH*TVMDNIYRSHYCI*RHQSQ 1292
DTQYVIG + +* N A++ + DN SHYC *+ Q+Q
Sbjct: 415 DTQYVIGDFDVLHV*PWNWWAYYLNMKDNKCYSHYCR*KRQNQ 287
Score = 37.3 bits (75), Expect(2) = e-109
Identities = 16/22 (72%), Positives = 19/22 (86%)
Frame = -1 / +2
Query: 1565 ARITCQGYQVLVQDILLSHAKK 1500
ARITCQGY +LVQD+LL AK+
Sbjct: 11 ARITCQGY*LLVQDLLL*VAKR 76
Score = 35.0 bits (70), Expect(5) = 2e-027
Identities = 14/21 (66%), Positives = 16/21 (76%)
Frame = +1 / -3
Query: 1012 MIWINHSKCKTIC*RVFRIFK 1074
MIWI H KC +I R+FRIFK
Sbjct: 567 MIWIVHFKCMSIGRRMFRIFK 505
Score = 35.0 bits (70), Expect(2) = 3e-056
Identities = 14/21 (66%), Positives = 15/21 (71%)
Frame = -2 / +3
Query: 1564 QELLVKDIKSWSKTSCFRMQR 1502
QE LVKD SW K SC+R QR
Sbjct: 12 QESLVKDTDSWCKISCYR*QR 74
Score = 32.2 bits (64), Expect(4) = 1e-030
Identities = 13/22 (59%), Positives = 16/22 (72%)
Frame = -3 / +1
Query: 1563 KNYLSRISSLGPRHPAFACKED 1498
KN+LSRI +LG R PA KE+
Sbjct: 13 KNHLSRILTLGARSPAIGSKEE 78
Score = 31.3 bits (62), Expect(2) = 2e-082
Identities = 15/22 (68%), Positives = 17/22 (77%)
Frame = +1 / -3
Query: 1504 FACESRMSWTKT*YP*QVILAM 1569
FA SR S TK+ YP*QVILA+
Sbjct: 72 FATYSRRSCTKSQYP*QVILAL 7
Score = 30.9 bits (61), Expect(4) = 3e-041
Identities = 16/24 (66%), Positives = 17/24 (70%)
Frame = +2 / -1
Query: 1493 LWSSLHAKAGCLGPRLDILDK*FL 1564
L SSL AG L PR+ ILDK*FL
Sbjct: 83 LLSSLLPIAGDLAPRVSILDK*FL 12
Score = 29.5 bits (58), Expect(5) = 2e-027
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = +3 / -2
Query: 1503 LCMRKQDVLDQDLISLTSNSC 1565
LC Q++L Q+ +SLTS+SC
Sbjct: 73 LCYL*QEILHQESVSLTSDSC 11
Score = 25.8 bits (50), Expect(4) = 1e-030
Identities = 17/26 (65%), Positives = 17/26 (65%)
Frame = -1 / +2
Query: 1472 SIYLSLL*TLLKRKI*FKLSETLLRL 1395
SI L L TLL RKI* SE LLRL
Sbjct: 107 SI*LLFLWTLLTRKI*SG*SEMLLRL 184
Score = 22.6 bits (43), Expect(5) = 2e-027
Identities = 14/25 (56%), Positives = 17/25 (68%)
Frame = +3 / -2
Query: 1398 PQQSF**FELNLPF*QSLQQ**IYA 1472
PQ F* L+LP *QS Q+* +YA
Sbjct: 181 PQ*HF*LSGLDLPC*QSPQK*QLYA 107
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 22,672,057
Number of Sequences: 8143
Number of extensions: 350027
Number of successful extensions: 1410
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 45
effective length of database: 1,201,052
effective search space used: 677393328
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)