TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3054736.2.1
(725 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815040.1|CN815040 HRO4505_B12_C23ZS5 Lib AA071E1X Aven... 245 7e-066
>gb|CN815040.1|CN815040 HRO4505_B12_C23ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4505_B12_C23, mRNA sequence
Length = 774
Score = 245 bits (529), Expect = 7e-066
Identities = 98/118 (83%), Positives = 107/118 (90%)
Frame = +3 / +2
Query: 3 VVNDPNLKWYESSWELLGNEYFMHASGSLYALSSEVVEALATAKSDSLRMFDYEDVTIGA 182
VV+DPN+KWYESS LLGNEYF HASG+LYALSSEVV LATAK+DSLRMFD+EDVTIG+
Sbjct: 59 VVSDPNMKWYESSCGLLGNEYFTHASGALYALSSEVVGTLATAKNDSLRMFDHEDVTIGS 238
Query: 183 WMLAMNVKHEDNRAMCDSVCTPTSIAVWDSKKCSGTCNVADKIKQLHNTTVCSKSPTL 356
WMLAMNV HEDNRAMCDS CTPTSIAVWDSK CSG+CN +KIK LHNTT+CSKSPTL
Sbjct: 239 WMLAMNVNHEDNRAMCDSTCTPTSIAVWDSKPCSGSCNPIEKIKGLHNTTLCSKSPTL 412
Score = 226 bits (488), Expect = 3e-060
Identities = 101/128 (78%), Positives = 105/128 (82%)
Frame = -2 / -1
Query: 385 FYXXXXXSGGNVGLFEHTVVLWSCFILSATLQVPEHFLLSHTAMEVGVHTESHIARLSSC 206
FY SGGNVGLFEH VVL S FI S LQ PEH LLSHTAMEVGVH ESHIARLSS
Sbjct: 441 FYSSSSTSGGNVGLFEHKVVLCSPFIFSMGLQEPEHGLLSHTAMEVGVHVESHIARLSS* 262
Query: 205 FTFIASIHAPIVTSS*SNILKLSLFAVARASTTSEERAYSEPDACMKYSLPNSSQELSYH 26
FTF+ASIH P+VTSS*SNILKLS FAVAR TTSEERAYS PDAC+KYSLPN+ ELSYH
Sbjct: 261 FTFMASIHEPMVTSS*SNILKLSFFAVARVPTTSEERAYSAPDACVKYSLPNNPHELSYH 82
Query: 25 FKFGSLTT 2
F FGSLTT
Sbjct: 81 FIFGSLTT 58
Score = 156 bits (336), Expect = 3e-039
Identities = 74/128 (57%), Positives = 87/128 (67%)
Frame = -3 / -2
Query: 384 SILPPPPQVATLGSLSTQWCYGAALFCQRRYKYLSISYCPIQRWR*GYILNHTLLGCLHA 205
S LPPP QV LG ST+ A LF Q Y+ L+++ CPIQ+WR + NHTL GCLH
Sbjct: 440 STLPPPHQVVMLGFSSTKLYCAAPLFSQWGYRNLNMACCPIQQWRWECMWNHTLHGCLHD 261
Query: 204 LHS*QASMRRL*HLHNQTSLSYHSLQ*LELQPPQKKEHIVNQMHA*STRYPTVPKNFRTI 25
LHS +ASM + *H ++TSL+YHSLQ*LE P KKEH V QM A*ST Y + NF TI
Sbjct: 260 LHSWRASMSQW*HPRDRTSLNYHSLQ*LEFPPLPKKEHTVRQMRA*STHYLIIHMNFHTI 81
Query: 24 SNLDH*QL 1
S LDH*QL
Sbjct: 80 SYLDH*QL 57
Score = 155 bits (332), Expect = 1e-038
Identities = 67/119 (56%), Positives = 84/119 (70%)
Frame = -1 / -3
Query: 359 WQRWAL*AHSGVMELLYFVSDVTST*AFLTVPYSDGGRGTY*ITHCSVVFMLYIHSKHPC 180
W WA A S +++ LYF++ VT T* +L VPYS+GG ITHC+VVFM+YIH +HP
Sbjct: 415 W*CWAFRAQSCIVQPLYFLNGVTGT*TWLAVPYSNGGGSACGITHCTVVFMIYIHGEHP* 236
Query: 179 ADCNIFIIKHP*AITLCSS*SFNHLRRKSI**TRCMHEVLVTQQFPRTFVPFQIWIIDN 3
A+ NI +I+HP* I LCSS*S +H RRKSI RC+ EVL+T TF+PF IWI DN
Sbjct: 235 ANGNILVIEHP*TIILCSS*SSHHFRRKSIQCARCVREVLIT**ST*TFIPFHIWITDN 59
Score = 140 bits (301), Expect = 2e-034
Identities = 67/128 (52%), Positives = 84/128 (65%)
Frame = +2 / +1
Query: 2 SCQ*SKFEMVRKFLGTVG*RVLHACIWFTICXXXXXXXXXXYCKE**LKDV*L*RCYNRR 181
SCQ*SK+EMV KF+ + * VLHA IW T+C YCKE** KDV CY+
Sbjct: 58 SCQ*SKYEMV*KFMWIIR**VLHARIWRTVCSFFGSGGNSSYCKE**FKDVRSRGCYHWL 237
Query: 182 MDACYECKA*RQPSNV*FSMYPYLHRCMGQ*EMLRYL*RR*QNKAAP*HHCVLKEPNVAT 361
MDA +ECK+*RQP NV*F M+ +LH CMGQ M R+L* *+NK A ++ VL++PN+ T
Sbjct: 238 MDARHECKS*RQPCNV*FHMHSHLHCCMGQQAMFRFL*PH*ENKGAAQYNFVLEKPNITT 417
Query: 362 XXXXXRIE 385
R+E
Sbjct: 418 *CGGGRVE 441
Score = 67.5 bits (141), Expect(3) = 1e-022
Identities = 31/52 (59%), Positives = 35/52 (67%)
Frame = +1 / +3
Query: 130 QRVIA*GCLIMKMLQSAHGCLL*M*SMKTTEQCVIQYVPLPPSLYGTVRNAQ 285
QR+I *GC I +ML AHGC *M* MKTT QCVI + PP LYGT + Q
Sbjct: 186 QRMIV*GCSITRMLPLAHGCSP*M*IMKTTVQCVIPHALPPPLLYGTASHVQ 341
Score = 35.9 bits (72), Expect(3) = 1e-022
Identities = 19/31 (61%), Positives = 21/31 (67%)
Frame = +1 / +3
Query: 1 QLSMIQI*NGTKVLGNCWVTSTSCMHLVHYM 93
QLS+IQI*NG KV + V STS HL H M
Sbjct: 57 QLSVIQI*NGMKVHVDY*VMSTSRTHLAHCM 149
Score = 35.0 bits (70), Expect(3) = 1e-022
Identities = 12/18 (66%), Positives = 15/18 (83%)
Frame = +1 / +3
Query: 331 LCAQRAQRCHLRWRRKNR 384
LCA++AQ HL WRRK+R
Sbjct: 387 LCARKAQHYHLMWRRKSR 440
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,365,538
Number of Sequences: 8143
Number of extensions: 136465
Number of successful extensions: 1394
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 241032924
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)