TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3042568.2.1
(1174 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN818432.1|CN818432 HRO4460_F09_K18ZS5 Lib AA070E1X Aven... 152 3e-050
gb|CN821434.1|CN821434 HRO4425_C07_E13ZS5 Lib AA069E1X Aven... 102 4e-046
gb|CN821039.1|CN821039 HRO4422_H02_O04ZS5 Lib AA069E1X Aven... 117 3e-027
>gb|CN818432.1|CN818432 HRO4460_F09_K18ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4460_F09_K18, mRNA sequence
Length = 869
Score = 152 bits (327), Expect(4) = 3e-050
Identities = 59/98 (60%), Positives = 77/98 (78%)
Frame = -1 / +3
Query: 541 LSVRDVFASILAFVPTGWGIISIAMAWKPVVKKLGLWKTVRALARLYDAGMGMVIFIPIA 362
+++RD+F ILAF+PTGWGI+ IA A +PV++ GLW +VRALAR Y+ MG+++F PI
Sbjct: 390 MTIRDIFVCILAFLPTGWGILLIAQACRPVLRVAGLWGSVRALARAYEVIMGVLLFTPIT 569
Query: 361 ICSWFPFISTFQTRLLFNQAFSRGLEISLILAGNNPNA 248
+ SWFPF+S FQTR+LFNQAFSRGL+IS IL G A
Sbjct: 570 VLSWFPFVSEFQTRMLFNQAFSRGLQISRILGGQKERA 683
Score = 98.2 bits (208), Expect = 2e-021
Identities = 49/121 (40%), Positives = 60/121 (49%)
Frame = +3 / -2
Query: 264 PARIRDISKPLLNAWLNRSRVWKVDMNGNHEQMAMGMKMTIPIPASXXXXXXXTVFHKPN 443
P RIR+I PLLNA LN RVW + GNH++ MG+K + P+ S T H P
Sbjct: 667 PPRIREI*SPLLNA*LNSIRVWNSETKGNHDRTVMGVKSSTPMITSYARARARTDPHSPA 488
Query: 444 FFTTGFHAMAIEMIPHPVGTNARMEAKTSLTLSFVNKIARTRAAKTINNATLLTKRRNNW 623
TG A AI IPHPVG NARM+ S + I T A N R+N+
Sbjct: 487 TRRTGLQACAISSIPHPVGKNARMQTNISRIVM*RMSINTTSTAANATNIMNFISRKNS* 308
Query: 624 K 626
K
Sbjct: 307 K 305
Score = 46.4 bits (95), Expect(4) = 3e-050
Identities = 16/33 (48%), Positives = 25/33 (75%)
Frame = -1 / +3
Query: 808 GRILETILSLRFFIFQFGVVYHMNASGGSTALL 710
G + E ILSLRFF++Q+G+VYH+N + + L+
Sbjct: 123 GNLFEIILSLRFFLYQYGLVYHLNITDDKSILV 221
Score = 29.5 bits (58), Expect(4) = 3e-050
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -3 / +2
Query: 884 WYWC*RRRKLGSLVGRR 834
W WC R++LG LVG R
Sbjct: 41 WDWCVTRKELGILVGER 91
Score = 28.6 bits (56), Expect(4) = 3e-050
Identities = 9/15 (60%), Positives = 10/15 (66%)
Frame = -1 / +3
Query: 916 RDWTNWLFYRGGIGV 872
RDW W+ GGIGV
Sbjct: 9 RDWNKWISNSGGIGV 53
>gb|CN821434.1|CN821434 HRO4425_C07_E13ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4425_C07_E13, mRNA
sequence
Length = 435
Score = 102 bits (218), Expect(2) = 4e-046
Identities = 45/84 (53%), Positives = 54/84 (64%)
Frame = -2 / +1
Query: 705 IGFLGLCLVAFSSSSWSLV*TPRPWYISNCSCVWSKV*RY*WFWLLWSWQFCLRNSV*EM 526
+G LG F SS W LV*TP PW+ISN CV SKV *WFWL+ WQ CL++ V +M
Sbjct: 181 LGILGCAWRCFLSS*WYLV*TPGPWFISNYCCVLSKVLPC*WFWLV*LWQLCLQDLVLQM 360
Query: 525 FLLPF*HLCQLGGESFRLPWHGNL 454
+LL F H+ L GE FR W G+L
Sbjct: 361 YLLSFWHMFLLDGEFFRSLWRGSL 432
Score = 102 bits (217), Expect(2) = 3e-044
Identities = 40/74 (54%), Positives = 49/74 (66%)
Frame = -3 / +2
Query: 674 FHPPLGLWFKPQGHGTFPIVPAFGQKCSVIDGFGCSGPGNFVYETQCERCFCFHSSICAN 495
F+PP G+ FKPQGHG+FP A QK ++GFG GN VY+T RC CFHS IC+
Sbjct: 212 FYPPNGI*FKPQGHGSFPTTAASCQKYCPVNGFGWFNCGNCVYKT*FYRCTCFHSGICSY 391
Query: 494 WVGNHFDCHGMETC 453
W+GN FD G+E C
Sbjct: 392 WMGNSFDRCGVEAC 433
Score = 101 bits (216), Expect(2) = 1e-042
Identities = 44/65 (67%), Positives = 51/65 (78%)
Frame = +3 / -3
Query: 453 TGFHAMAIEMIPHPVGTNARMEAKTSLTLSFVNKIARTRAAKTINNATLLTKRRNNWKCT 632
TGFHA AIE IPHPVGT ARM+A TS+ LS V+ IA + AKTIN A LLT+R ++WK T
Sbjct: 433 TGFHATAIERIPHPVGTYARMKASTSVKLSLVDTIATIKPAKTINRAILLTRRSSSWK*T 254
Query: 633 MALGF 647
MALGF
Sbjct: 253 MALGF 239
Score = 97.7 bits (207), Expect(2) = 4e-046
Identities = 43/65 (66%), Positives = 48/65 (73%)
Frame = -1 / +2
Query: 883 GIGVKGXXXXXXXXXXELQHIYSIRGRILETILSLRFFIFQFGVVYHMNASGGSTALLVY 704
G VKG EL HI + RGRILETILSLRFFIFQ+GVVYHM+AS ST+LLVY
Sbjct: 2 GTRVKGDASWEAWWDEELGHIQTFRGRILETILSLRFFIFQYGVVYHMDASEPSTSLLVY 181
Query: 703 WISWA 689
W+SWA
Sbjct: 182 WVSWA 196
Score = 92.3 bits (195), Expect(2) = 3e-044
Identities = 39/64 (60%), Positives = 45/64 (70%)
Frame = -3 / +1
Query: 872 *RRRKLGSLVGRRTATYLQHSREDTGNHIKPEVFHFPIRSCLPHEC*RRKYSFIGVLDFL 693
*RR KLGSLVG RT TY EDTG+H ++FHFP+ SCLP+ C R KY IG+L L
Sbjct: 13 *RRCKLGSLVG*RTGTYPNLPWEDTGDHT*FKIFHFPVWSCLPYGCQRTKYIIIGILGIL 192
Query: 692 GCAW 681
GCAW
Sbjct: 193 GCAW 204
Score = 87.2 bits (184), Expect(2) = 1e-042
Identities = 38/67 (56%), Positives = 44/67 (65%)
Frame = +3 / -1
Query: 684 STAQEIQYTNKAVLPPLAFMW*TTPNWKMKNLRLNMVSSILPRML*ICCXXXXXXXXXXX 863
STAQ+ QYTN VL LA +W*TTP WKMKNL+L+MVSSILPR + IC
Sbjct: 201 STAQDTQYTNNDVLGSLASIW*TTPYWKMKNLKLSMVSSILPRKVWICPSSSSHQASQLA 22
Query: 864 XPLTPIP 884
PLT +P
Sbjct: 21 SPLTLVP 1
Score = 84.5 bits (178), Expect(2) = 3e-032
Identities = 41/74 (55%), Positives = 47/74 (63%)
Frame = +1 / -1
Query: 454 QVSMPWQSK*FPTQLAQMLEWKQKHLSH*VS*TKLPGPEQPKPSITLHF*PNAGTIGNVP 633
Q S P +SK FP Q M E KQ HL +*V *T+LP QPKP +F* +A +GN P
Sbjct: 432 QASTPQRSKEFPIQ*EHMPE*KQVHL*N*VL*TQLPQLNQPKPLTGQYF*QDAAVVGNEP 253
Query: 634 WPWGLNQRPRGG*K 675
WPWGLN P GG*K
Sbjct: 252 WPWGLN*IPLGG*K 211
Score = 80.8 bits (170), Expect(2) = 1e-026
Identities = 40/83 (48%), Positives = 51/83 (61%)
Frame = +2 / -2
Query: 455 RFPCHGNRNDSPPSWHKC*NGSKNISHTEFRKQNCQDQSSQNHQ*RYTFDQTQEQLEMYH 634
R P H +R +SP S + C N SK I T+ + NC + +SQNH TFD+TQ+ LEM H
Sbjct: 431 RLPRHSDRKNSPSSRNICQNESKYICKTKSCRHNCHN*TSQNH*QGNTFDKTQQ*LEMNH 252
Query: 635 GLGV*TKDQEEDEKATKHSPRNP 703
G GV*TK ED+K + PR P
Sbjct: 251 GPGV*TKYH*EDKKHLQAQPRIP 183
Score = 69.8 bits (146), Expect(2) = 3e-032
Identities = 30/54 (55%), Positives = 37/54 (68%)
Frame = +2 / -3
Query: 671 EKATKHSPRNPIHQ*SCTSSASIHVVNNSELENEKPQA*YGFQYPPSNAVNMLQ 832
+ +KHSP PI+Q* CT A IH+VNNS LENEK YG QYPP+ ++M Q
Sbjct: 214 KSTSKHSPGYPIYQ**CTWFAGIHMVNNSILENEKS*TKYGLQYPPTEGLDMSQ 53
Score = 65.2 bits (136), Expect(3) = 5e-028
Identities = 28/51 (54%), Positives = 33/51 (64%)
Frame = -2 / +3
Query: 834 NCNIFTAFEGGYWKPY*A*GFSFSNSELFTT*MLAEEVQLYWCIGFLGLCL 682
N +I GGYW+PY * FSFS+ ELFT M A +V YW IG+ GLCL
Sbjct: 51 NWDISKPSVGGYWRPYLV*DFSFSSMELFTIWMPANQVHHYWYIGYPGLCL 203
Score = 62.0 bits (129), Expect(3) = 5e-028
Identities = 24/32 (75%), Positives = 28/32 (87%)
Frame = -1 / +3
Query: 547 TKLSVRDVFASILAFVPTGWGIISIAMAWKPV 452
T+LS DV A ILA+VPTGWGI+SIA+AWKPV
Sbjct: 339 TRLSFTDVLAFILAYVPTGWGILSIAVAWKPV 434
Score = 54.2 bits (112), Expect(2) = 1e-026
Identities = 26/51 (50%), Positives = 30/51 (58%)
Frame = +1 / -2
Query: 682 QAQPKKSNTPIKLYFLR*HSCGKQLRIGK*KTSGLIWFPVSSLECCKYVAV 834
QAQP+ N PI +Y +R H GKQL GK*K +W PVSS YV V
Sbjct: 203 QAQPRIPNIPIMMYLVRWHPYGKQLHTGK*KILN*VWSPVSSHGRFGYVPV 51
Score = 30.4 bits (60), Expect(3) = 5e-028
Identities = 11/13 (84%), Positives = 12/13 (92%)
Frame = -1 / +3
Query: 646 NPKAMVHFQLFLR 608
NP+AMVHFQL LR
Sbjct: 240 NPRAMVHFQLLLR 278
>gb|CN821039.1|CN821039 HRO4422_H02_O04ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4422_H02_O04, mRNA
sequence
Length = 700
Score = 117 bits (251), Expect = 3e-027
Identities = 43/94 (45%), Positives = 63/94 (67%)
Frame = -1 / +2
Query: 544 KLSVRDVFASILAFVPTGWGIISIAMAWKPVVKKLGLWKTVRALARLYDAGMGMVIFIPI 365
KLS+ D+ LAF+PTGWG++ I +P ++ +W+ ++ +A YD GMG ++F PI
Sbjct: 86 KLSIMDLIICCLAFIPTGWGLLLIVQVLRPKIEYYAIWEPIQVIAHAYDYGMGSLLFFPI 265
Query: 364 AICSWFPFISTFQTRLLFNQAFSRGLEISLILAG 263
AI +W P IS QTR+LFN+AFSR L+I + G
Sbjct: 266 AILAWMPIISAIQTRVLFNRAFSRQLQIQPFIVG 367
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 14,700,770
Number of Sequences: 8143
Number of extensions: 232707
Number of successful extensions: 1162
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 418381470
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)