TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2921718.2.1
(649 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN817629.1|CN817629 HRO4463_B12_C23ZS5 Lib AA070E1X Aven... 260 1e-070
gb|CN816486.1|CN816486 HRO4521_C09_E17ZS5 Lib AA071E1X Aven... 225 5e-060
>gb|CN817629.1|CN817629 HRO4463_B12_C23ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4463_B12_C23, mRNA sequence
Length = 781
Score = 260 bits (563), Expect = 1e-070
Identities = 103/124 (83%), Positives = 117/124 (94%)
Frame = +2 / +2
Query: 5 RHEARLASGAHVLGNVLVHETAVIGDGCLIGPDVAVGPGCVVEAGVRLSRCTVMRAARVK 184
R A+LA+G HV+GNVLVHE+A IG+GCLIGPDVA+GPGCVVE GVRLSRCTVMR AR+K
Sbjct: 233 RSAAKLATGTHVVGNVLVHESAKIGEGCLIGPDVAIGPGCVVEDGVRLSRCTVMRGARIK 412
Query: 185 QHACVSSSIIGWHSTVGKWARVENMTILGEDVHVCDEIYSNGGVVLPHKEIKSSILKPEI 364
+HAC+S+SI+GWHSTVGKWAR+ENMTILGEDVHV DE+YSNGGVVLPHKEIKSSILKPEI
Sbjct: 413 KHACISNSIVGWHSTVGKWARIENMTILGEDVHVGDEVYSNGGVVLPHKEIKSSILKPEI 592
Query: 365 VM*S 376
VM*+
Sbjct: 593 VM*A 604
Score = 207 bits (446), Expect = 2e-054
Identities = 87/121 (71%), Positives = 96/121 (79%)
Frame = -2 / -2
Query: 372 HMTISGLRMLDLISLCGSTTPPLL*ISSQTWTSSPRMVMFSTRAHLPTVECQPMMLLETQ 193
HMTISG RMLDLISLCG TTPPLL* SS T TSSPR+V+FS RAH PTVEC P ML E Q
Sbjct: 600 HMTISGFRMLDLISLCGRTTPPLL*TSSPTCTSSPRIVIFSMRAHFPTVECHPTMLFEMQ 421
Query: 192 ACCLTRAARMTVQRDRRTPASTTQPGPTATSGPMRQPSPMTAVSCTSTLPRTCAPDASLA 13
AC L RA R+TV R+ TP+STT PGP ATSGP+RQPSP+ A+SCT T P TC P ASLA
Sbjct: 420 ACFLIRAPRITVHRESLTPSSTTHPGPIATSGPIRQPSPILALSCTKTFPTTCVPVASLA 241
Query: 12 S 10
+
Sbjct: 240 A 238
>gb|CN816486.1|CN816486 HRO4521_C09_E17ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4521_C09_E17, mRNA sequence
Length = 431
Score = 225 bits (486), Expect = 5e-060
Identities = 88/101 (87%), Positives = 98/101 (97%)
Frame = +2 / +2
Query: 74 IGDGCLIGPDVAVGPGCVVEAGVRLSRCTVMRAARVKQHACVSSSIIGWHSTVGKWARVE 253
IG+GCLIGPDVA+GPGCVVE GVRLSRCTVMR AR+K+HAC+S+SI+GWHSTVGKWAR+E
Sbjct: 2 IGEGCLIGPDVAIGPGCVVEDGVRLSRCTVMRGARIKKHACISNSIVGWHSTVGKWARIE 181
Query: 254 NMTILGEDVHVCDEIYSNGGVVLPHKEIKSSILKPEIVM*S 376
NMTILGEDVHV DE+YSNGGVVLPHKEIKSSILKPEIVM*+
Sbjct: 182 NMTILGEDVHVGDEVYSNGGVVLPHKEIKSSILKPEIVM*A 304
Score = 176 bits (378), Expect = 4e-045
Identities = 74/100 (74%), Positives = 81/100 (81%)
Frame = -2 / -3
Query: 372 HMTISGLRMLDLISLCGSTTPPLL*ISSQTWTSSPRMVMFSTRAHLPTVECQPMMLLETQ 193
HMTISG RMLDLISLCG TTPPLL* SS T TSSPR+V+FS RAH PTVEC P ML E Q
Sbjct: 300 HMTISGFRMLDLISLCGRTTPPLL*TSSPTCTSSPRIVIFSMRAHFPTVECHPTMLFEMQ 121
Query: 192 ACCLTRAARMTVQRDRRTPASTTQPGPTATSGPMRQPSPM 73
AC L RA R+TV R+ TP+STT PGP ATSGP+RQPSP+
Sbjct: 120 ACFLIRAPRITVHRESLTPSSTTHPGPIATSGPIRQPSPI 1
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,319,302
Number of Sequences: 8143
Number of extensions: 90528
Number of successful extensions: 1457
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 212008213
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)