TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2762998.2.1
(1036 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN818607.1|CN818607 HRO4467_B12_C23ZS5 Lib AA070E1X Aven... 210 2e-084
gb|CN817232.1|CN817232 HRO4464_D08_G16ZS5 Lib AA070E1X Aven... 129 6e-031
>gb|CN818607.1|CN818607 HRO4467_B12_C23ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4467_B12_C23, mRNA sequence
Length = 861
Score = 210 bits (454), Expect(2) = 2e-084
Identities = 90/159 (56%), Positives = 115/159 (72%)
Frame = -2 / +2
Query: 948 VXXXXELKXXXXMFPIWATAIIFSAVYAQNSSMFIEQGMVLDKRIGSFNIPPASLSTFDV 769
V ELK MFPIW+ ++F AV AQ S FIEQ M +++ +GSF IP AS + +
Sbjct: 83 VTQVEELKILIRMFPIWSAMVLFCAVQAQMFSTFIEQAMAMERHVGSFEIPAASFQSVNT 262
Query: 768 ISVIMWVPLYDRILVPLARKFTGREKGFSELQRMGIGLVLSILAMVSAALVELKRLEIAR 589
ISVI+ VP+Y+R+L+P+ RKFTGR G + QR+GIGL S+ +MVSAALVE RL+IA+
Sbjct: 263 ISVIILVPVYERVLIPVFRKFTGRANGITTPQRIGIGLFFSMSSMVSAALVESTRLQIAQ 442
Query: 588 SEGLIHEKAAVPMSILWQIPQYFLVGAAEVFTCIGQVEF 472
+EGL+H K AVPMSILWQ PQYFL+G AEVF+ IG F
Sbjct: 443 AEGLVHRKVAVPMSILWQGPQYFLLGIAEVFSNIGLTVF 559
Score = 162 bits (348), Expect(2) = 6e-058
Identities = 78/150 (52%), Positives = 93/150 (62%)
Frame = +2 / -1
Query: 485 PIQVNTSAAPTKKYCGICQRMLIGTAAFS*MRPSDLAISKRFNSTRAADTIARMDRTNPI 664
P+ NTSA P++KY G C RMLIGTA +PS AI R ST AADTI M++ PI
Sbjct: 546 PMLENTSAIPSRKYWGPCHRMLIGTATLRCTKPSACAICNRVLSTNAADTIEDMEKNKPI 367
Query: 665 PIRCSSEKPFSLPVNFLASGTRMRS*SGTHMMTLITSKVERDAGGMLKDPMRLSRTMPCS 844
PIRC PF+LPVNFL +G R S*+GT +MT I +DA G+ KDP LS + CS
Sbjct: 366 PIRCGVVMPFALPVNFLNTGIRTLS*TGTSIMTEIVFTDWKDAAGISKDPTCLSIAIACS 187
Query: 845 MNMEEFWA*TALNMIAVAQMGNIXXXIFNS 934
MN+E A*TA N +GNI IFNS
Sbjct: 186 MNVENICA*TAQNNTIADHIGNILIRIFNS 97
Score = 117 bits (250), Expect(2) = 2e-084
Identities = 44/72 (61%), Positives = 58/72 (80%)
Frame = -2 / +3
Query: 474 FFYDQAPDAMRSLCSALALITVSLGNYISSIILTLVSYITTQGGDPGWIPDNLNEGHLDR 295
FFYD++PDAMRSLC A ++ + S+GNY+SS IL LV T +GG PGWIPDNLNEGHLDR
Sbjct: 555 FFYDESPDAMRSLCVAFSVASDSVGNYLSSFILYLVPVFTARGGSPGWIPDNLNEGHLDR 734
Query: 294 FFWLIAGISFVD 259
+ ++AG+S ++
Sbjct: 735 IYLMMAGLSLLN 770
Score = 96.4 bits (204), Expect = 7e-021
Identities = 50/147 (34%), Positives = 82/147 (55%)
Frame = +1 / -3
Query: 487 NTSKHLSSAHQEILWYLPKNAHWNSSLLMNETFRPGNL*TLQLN*SCRYHRENGQD*SNS 666
N +HL ++ QE+L LP++AH NS +++TF NL* L C +H +G+ *+N
Sbjct: 544 NVGEHLGNS*QEVLGSLPQDAHRNSHFAVHQTFSLCNL*PSTLYQCC*HH*GHGEK*TNP 365
Query: 667 HPL*LRKTLLPSSEFSS*WHQDAVIEWDPHDDADYIKSGERCRRNVERPNALVKNHALLY 846
+PL R + +S+FS ++D++I W+ ++D + I E C R +RP+ + H LLY
Sbjct: 364 YPLRCRNAICSASKFSKYRNKDSLINWN*YNDRNCIYRLEGCSRYFKRPDMSLHCHRLLY 185
Query: 847 EHGRVLGINSTEYDSSSPNGKHXXXXL 927
E + L +N T+ P +H L
Sbjct: 184 EC*KHLCLNCTKQYHCRPYWEHSDKNL 104
Score = 77.6 bits (163), Expect(2) = 6e-058
Identities = 40/71 (56%), Positives = 49/71 (69%)
Frame = +2 / -3
Query: 266 KLIPAINQKNRSRWPSFRLSGIHPGSPP*VVMYDTNVSMMELI*FPSETVISASALHKLL 445
KL PAI + S+ PSF+LSGIHPG PP V T + EL *FP+E++ + +A HKLL
Sbjct: 763 KLNPAIIR*ILSKCPSFKLSGIHPGLPPLAVNTGTR*RINELR*FPTESLATENATHKLL 584
Query: 446 MASGA*S*KNS 478
MASG S*KNS
Sbjct: 583 MASGDSS*KNS 551
>gb|CN817232.1|CN817232 HRO4464_D08_G16ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4464_D08_G16, mRNA sequence
Length = 782
Score = 129 bits (277), Expect = 6e-031
Identities = 49/102 (48%), Positives = 72/102 (70%)
Frame = -2 / +3
Query: 564 AAVPMSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCSALALITVSLGNYISS 385
A +PMS+ W +PQY L+GAA+VFT +G EFFYDQ PDA +SL AL L + +G++ISS
Sbjct: 156 AVIPMSLWWIMPQYLLIGAADVFTMVGMQEFFYDQMPDAFKSLGLALYLSVLGVGSFISS 335
Query: 384 IILTLVSYITTQGGDPGWIPDNLNEGHLDRFFWLIAGISFVD 259
+++++ +T + G W DNLN GHLD F+ LIA ++ ++
Sbjct: 336 FLISVIDGVTRRDGGTSWFADNLNRGHLDYFYLLIAALTALE 461
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 12,130,580
Number of Sequences: 8143
Number of extensions: 189096
Number of successful extensions: 1194
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 362758500
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)