TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2591248.2.1
(1222 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN821037.1|CN821037 HRO4422_G12_M24ZS5 Lib AA069E1X Aven... 80 2e-029
gb|CN821305.1|CN821305 HRO4423_G12_N23ZS5 Lib AA069E1X Aven... 80 3e-029
gb|CN820388.1|CN820388 HRO4418_G08_M16ZS5 Lib AA069E1X Aven... 80 3e-024
gb|CN821108.1|CN821108 HRO4427_F02_L03ZS5 Lib AA069E1X Aven... 81 2e-022
gb|CN821143.1|CN821143 HRO4428_A02_B04ZS5 Lib AA069E1X Aven... 77 3e-021
>gb|CN821037.1|CN821037 HRO4422_G12_M24ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4422_G12_M24, mRNA
sequence
Length = 784
Score = 80.3 bits (169), Expect(4) = 2e-029
Identities = 27/55 (49%), Positives = 40/55 (72%)
Frame = -1 / +2
Query: 679 VSCGSSSASPVQLRRVAKRQVDYILGDNPLRMSYMVGYGARFPRRIHHRASSLPS 515
V C SP +R A Q +YILGDNP+++SY+VGYG+ +P+++HHR +S+PS
Sbjct: 182 VECSGKYFSPTDIRNFAISQANYILGDNPMKLSYLVGYGSNYPQQVHHRGASIPS 346
Score = 35.9 bits (72), Expect(4) = 2e-029
Identities = 13/23 (56%), Positives = 17/23 (73%)
Frame = -1 / +2
Query: 478 GAAYYTSPAPNPNLLVGAVVGGP 410
G Y S +PNPN+ +GA+VGGP
Sbjct: 368 GFDYLHSTSPNPNIAMGALVGGP 436
Score = 34.5 bits (69), Expect(4) = 2e-029
Identities = 12/29 (41%), Positives = 21/29 (72%)
Frame = -1 / +2
Query: 784 GGLLFKVGSSNMQHVTQLSFLLLAYSNYL 698
GGL++ G +++QH T +FL + YS+Y+
Sbjct: 74 GGLVWISGWNSLQHATNAAFLAVVYSDYM 160
Score = 28.6 bits (56), Expect(4) = 2e-029
Identities = 13/29 (44%), Positives = 19/29 (65%)
Frame = -1 / +2
Query: 400 SDAFPDARAVFQQSEPTTYINAPLMGLLA 314
+D+F D R Q+E +TY + L+GLLA
Sbjct: 443 NDSFVDLRDNAVQTESSTYNSGTLVGLLA 529
>gb|CN821305.1|CN821305 HRO4423_G12_N23ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4423_G12_N23, mRNA
sequence
Length = 815
Score = 79.9 bits (168), Expect(4) = 3e-029
Identities = 27/55 (49%), Positives = 40/55 (72%)
Frame = -1 / +1
Query: 679 VSCGSSSASPVQLRRVAKRQVDYILGDNPLRMSYMVGYGARFPRRIHHRASSLPS 515
V C SP +R A Q +YILGDNP+++SY+VGYG+ +P+++HHR +S+PS
Sbjct: 157 VECSGKYFSPTYIRNFAISQANYILGDNPMKLSYLVGYGSNYPQQVHHRGASIPS 321
Score = 35.9 bits (72), Expect(4) = 3e-029
Identities = 13/23 (56%), Positives = 17/23 (73%)
Frame = -1 / +1
Query: 478 GAAYYTSPAPNPNLLVGAVVGGP 410
G Y S +PNPN+ +GA+VGGP
Sbjct: 343 GFDYLHSTSPNPNIAMGALVGGP 411
Score = 34.5 bits (69), Expect(4) = 3e-029
Identities = 12/29 (41%), Positives = 21/29 (72%)
Frame = -1 / +1
Query: 784 GGLLFKVGSSNMQHVTQLSFLLLAYSNYL 698
GGL++ G +++QH T +FL + YS+Y+
Sbjct: 49 GGLVWISGWNSLQHATNAAFLAVVYSDYM 135
Score = 28.6 bits (56), Expect(4) = 3e-029
Identities = 13/29 (44%), Positives = 19/29 (65%)
Frame = -1 / +1
Query: 400 SDAFPDARAVFQQSEPTTYINAPLMGLLA 314
+D+F D R Q+E +TY + L+GLLA
Sbjct: 418 NDSFVDLRDNAVQTESSTYNSGTLVGLLA 504
>gb|CN820388.1|CN820388 HRO4418_G08_M16ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4418_G08_M16, mRNA
sequence
Length = 679
Score = 80.3 bits (169), Expect(3) = 3e-024
Identities = 27/55 (49%), Positives = 40/55 (72%)
Frame = -1 / +3
Query: 679 VSCGSSSASPVQLRRVAKRQVDYILGDNPLRMSYMVGYGARFPRRIHHRASSLPS 515
V C SP +R A Q +YILGDNP+++SY+VGYG+ +P+++HHR +S+PS
Sbjct: 90 VECSGKYFSPTDIRNFAISQANYILGDNPMKLSYLVGYGSNYPQQVHHRGASIPS 254
Score = 35.9 bits (72), Expect(3) = 3e-024
Identities = 13/23 (56%), Positives = 17/23 (73%)
Frame = -1 / +3
Query: 478 GAAYYTSPAPNPNLLVGAVVGGP 410
G Y S +PNPN+ +GA+VGGP
Sbjct: 276 GFDYLHSTSPNPNIAMGALVGGP 344
Score = 28.6 bits (56), Expect(3) = 3e-024
Identities = 13/29 (44%), Positives = 19/29 (65%)
Frame = -1 / +3
Query: 400 SDAFPDARAVFQQSEPTTYINAPLMGLLA 314
+D+F D R Q+E +TY + L+GLLA
Sbjct: 351 NDSFVDLRDNAVQTESSTYNSGTLVGLLA 437
>gb|CN821108.1|CN821108 HRO4427_F02_L03ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4427_F02_L03, mRNA
sequence
Length = 714
Score = 80.8 bits (170), Expect(2) = 2e-022
Identities = 29/57 (50%), Positives = 40/57 (70%)
Frame = -1 / +2
Query: 673 CGSSSASPVQLRRVAKRQVDYILGDNPLRMSYMVGYGARFPRRIHHRASSLPSVAAH 503
CG + LR+ AK Q+DYILG NP +MSY+VGYG ++P+R+HHR +S+P H
Sbjct: 221 CGPNFYKTDVLRKFAKSQIDYILGKNPQKMSYVVGYGKKYPKRVHHRGASIPHNGVH 391
Score = 40.5 bits (82), Expect(2) = 2e-022
Identities = 16/36 (44%), Positives = 21/36 (58%)
Frame = -1 / +2
Query: 517 SVAAHPARIGCKAGAAYYTSPAPNPNLLVGAVVGGP 410
S+ + GCK G + S NPN+LVGA+V GP
Sbjct: 368 SIPHNGVHYGCKGGFKWRESKKANPNILVGAMVAGP 475
>gb|CN821143.1|CN821143 HRO4428_A02_B04ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4428_A02_B04, mRNA
sequence
Length = 636
Score = 76.7 bits (161), Expect(2) = 3e-021
Identities = 26/46 (56%), Positives = 36/46 (78%)
Frame = -1 / +2
Query: 640 RRVAKRQVDYILGDNPLRMSYMVGYGARFPRRIHHRASSLPSVAAH 503
R+ AK Q+DYILG NP +MSY+VGYG ++P+R+HHR +S+P H
Sbjct: 11 RKFAKSQIDYILGKNPQKMSYVVGYGKKYPKRVHHRGASIPHNGVH 148
Score = 40.5 bits (82), Expect(2) = 3e-021
Identities = 16/36 (44%), Positives = 21/36 (58%)
Frame = -1 / +2
Query: 517 SVAAHPARIGCKAGAAYYTSPAPNPNLLVGAVVGGP 410
S+ + GCK G + S NPN+LVGA+V GP
Sbjct: 125 SIPHNGVHYGCKGGFKWRESKKANPNILVGAMVAGP 232
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,869,516
Number of Sequences: 8143
Number of extensions: 135437
Number of successful extensions: 951
Number of sequences better than 1.0e-020: 5
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 437728590
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)