TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2591032.2.1
(735 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN819189.1|CN819189 HRO4451_B10_C19ZS5 Lib AA070E1X Aven... 177 2e-045
gb|CN820364.1|CN820364 HRO4418_E06_I12ZS5 Lib AA069E1X Aven... 177 2e-045
gb|AF033096.1|AF033096 Avena sativa nonphototropic hypocoty... 171 2e-043
gb|AF033097.1|AF033097 Avena sativa nonphototropic hypocoty... 171 2e-043
gb|CN820184.1|CN820184 HRO4412_B04_D08ZS5 Lib AA069E1X Aven... 155 1e-038
gb|CN819412.1|CN819412 HRO4405_G02_M03ZS5 Lib AA069E1X Aven... 101 2e-027
gb|CN817518.1|CN817518 HRO4454_B04_D08ZS5 Lib AA070E1X Aven... 95 9e-021
>gb|CN819189.1|CN819189 HRO4451_B10_C19ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4451_B10_C19, mRNA sequence
Length = 791
Score = 177 bits (381), Expect = 2e-045
Identities = 64/125 (51%), Positives = 88/125 (70%)
Frame = -2 / +3
Query: 707 SMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGVFLYELLFGKTPFKGSSNRATLFNVIGQQ 528
S SFVGT EY+APE+I G GH SA+DWW G+ LYE+L+G+TPF+G + + T +N++ +
Sbjct: 30 SNSFVGTEEYIAPEVITGAGHTSAIDWWALGILLYEMLYGRTPFRGKNRKKTFYNILHKD 209
Query: 527 LRFPEYPAVSFSARDLIRGLLVKEPQQRLAYKRGATEIKQHPFFEGVNWALIRCASPPEV 348
L FP VS A+ LI GLL ++P R+ GA +IK+HPFFE + W LIRC SPPE+
Sbjct: 210 LTFPSSIPVSLEAKQLIHGLLQRDPSARIGSNTGANDIKEHPFFEDIYWPLIRCMSPPEL 389
Query: 347 PKPVE 333
P++
Sbjct: 390 DVPLK 404
Score = 102 bits (218), Expect = 6e-023
Identities = 55/123 (44%), Positives = 64/123 (52%)
Frame = +1 / -2
Query: 340 GFGTSGGLAHLMSAQFTPSKKGCCLISVAPRL*ASRCWGSFTSRPLIRSLAEKLTAGYSG 519
G +SGGL +S Q SK GC LIS AP R GS S P I A +LT G
Sbjct: 397 GTSSSGGLMQRISGQ*ISSKNGCSLISFAPVFEPMRAEGSL*SSPWINCFASRLTGMLLG 218
Query: 520 KRSCWPMTLNSVARLLDPLKGVFPNSSSYKNTPKVHQSTALPWPSPLMISGARYSCVPTN 699
L +V L P KGV P + SY P+ HQS AL P+P+M SGA YS VPTN
Sbjct: 217 NVRSLCRML*NVFFLFFPRKGVRPYNISYNRIPRAHQSMALVCPAPVMTSGAMYSSVPTN 38
Query: 700 DID 708
+ D
Sbjct: 37 EFD 29
>gb|CN820364.1|CN820364 HRO4418_E06_I12ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4418_E06_I12, mRNA
sequence
Length = 699
Score = 177 bits (381), Expect = 2e-045
Identities = 64/125 (51%), Positives = 88/125 (70%)
Frame = -2 / +3
Query: 707 SMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGVFLYELLFGKTPFKGSSNRATLFNVIGQQ 528
S SFVGT EY+APE+I G GH SA+DWW G+ LYE+L+G+TPF+G + + T +N++ +
Sbjct: 39 SNSFVGTEEYIAPEVITGAGHTSAIDWWALGILLYEMLYGRTPFRGKNRKKTFYNILHKD 218
Query: 527 LRFPEYPAVSFSARDLIRGLLVKEPQQRLAYKRGATEIKQHPFFEGVNWALIRCASPPEV 348
L FP VS A+ LI GLL ++P R+ GA +IK+HPFFE + W LIRC SPPE+
Sbjct: 219 LTFPSSIPVSLEAKQLIHGLLQRDPSARIGSNTGANDIKEHPFFEDIYWPLIRCMSPPEL 398
Query: 347 PKPVE 333
P++
Sbjct: 399 DVPLK 413
Score = 102 bits (218), Expect = 6e-023
Identities = 55/123 (44%), Positives = 64/123 (52%)
Frame = +1 / -3
Query: 340 GFGTSGGLAHLMSAQFTPSKKGCCLISVAPRL*ASRCWGSFTSRPLIRSLAEKLTAGYSG 519
G +SGGL +S Q SK GC LIS AP R GS S P I A +LT G
Sbjct: 406 GTSSSGGLMQRISGQ*ISSKNGCSLISFAPVFEPMRAEGSL*SSPWINCFASRLTGMLLG 227
Query: 520 KRSCWPMTLNSVARLLDPLKGVFPNSSSYKNTPKVHQSTALPWPSPLMISGARYSCVPTN 699
L +V L P KGV P + SY P+ HQS AL P+P+M SGA YS VPTN
Sbjct: 226 NVRSLCRML*NVFFLFFPRKGVRPYNISYNRIPRAHQSMALVCPAPVMTSGAMYSSVPTN 47
Query: 700 DID 708
+ D
Sbjct: 46 EFD 38
>gb|AF033096.1|AF033096 Avena sativa nonphototropic hypocotyl 1 (NPH1-1) mRNA, complete cds
Length = 3467
Score = 171 bits (367), Expect = 2e-043
Identities = 65/124 (52%), Positives = 87/124 (70%)
Frame = -2 / +2
Query: 707 SMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGVFLYELLFGKTPFKGSSNRATLFNVIGQQ 528
S SFVGT EY+APEII G GH SAVDWW G+ LYE+L+G TPF+G + + T N++ +
Sbjct: 2735 SNSFVGTEEYIAPEIITGAGHTSAVDWWALGILLYEMLYGYTPFRGKTRQRTFANILHKD 2914
Query: 527 LRFPEYPAVSFSARDLIRGLLVKEPQQRLAYKRGATEIKQHPFFEGVNWALIRCASPPEV 348
+RFP +VS AR LI LL ++P RL G+ EIK+HPFF G+NWAL+R +PP++
Sbjct: 2915 IRFPASISVSLPARQLIYRLLHRDPSNRLGSYEGSNEIKEHPFFRGINWALVRGTAPPKL 3094
Query: 347 PKPV 336
P+
Sbjct: 3095 DAPL 3106
Score = 110 bits (234), Expect = 3e-025
Identities = 56/125 (44%), Positives = 69/125 (55%)
Frame = +1 / -3
Query: 334 STGFGTSGGLAHLMSAQFTPSKKGCCLISVAPRL*ASRCWGSFTSRPLIRSLAEKLTAGY 513
++G + GG AQ P KKGC IS P S GS S I LA +LT
Sbjct: 3108 NSGASSFGGAVPRTRAQLMPRKKGCSFISFDPSYDPSLFEGSLCSSLYISCLAGRLTDIL 2929
Query: 514 SGKRSCWPMTLNSVARLLDPLKGVFPNSSSYKNTPKVHQSTALPWPSPLMISGARYSCVP 693
+G L +V L+ PLKGV P + SY+ P HQSTAL WP+P+MISGA YS VP
Sbjct: 2928 AGNLMSLCRMLANVLCLVLPLKGV*PYNISYRRIPSAHQSTALVWPAPVMISGAMYSSVP 2749
Query: 694 TNDID 708
TN++D
Sbjct: 2748 TNELD 2734
>gb|AF033097.1|AF033097 Avena sativa nonphototropic hypocotyl 1 (NPH1-2) mRNA, complete cds
Length = 3422
Score = 171 bits (367), Expect = 2e-043
Identities = 65/124 (52%), Positives = 87/124 (70%)
Frame = -2 / +2
Query: 707 SMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGVFLYELLFGKTPFKGSSNRATLFNVIGQQ 528
S SFVGT EY+APEII G GH SAVDWW G+ LYE+L+G TPF+G + + T N++ +
Sbjct: 2672 SNSFVGTEEYIAPEIITGAGHTSAVDWWALGILLYEMLYGYTPFRGKTRQRTFANILHKD 2851
Query: 527 LRFPEYPAVSFSARDLIRGLLVKEPQQRLAYKRGATEIKQHPFFEGVNWALIRCASPPEV 348
+RFP +VS AR LI LL ++P RL G+ EIK+HPFF G+NWAL+R +PP++
Sbjct: 2852 IRFPASISVSLPARQLIYRLLHRDPSNRLGSYEGSNEIKEHPFFRGINWALVRGTAPPKL 3031
Query: 347 PKPV 336
P+
Sbjct: 3032 DAPL 3043
Score = 107 bits (229), Expect = 2e-024
Identities = 55/125 (44%), Positives = 68/125 (54%)
Frame = +1 / -3
Query: 334 STGFGTSGGLAHLMSAQFTPSKKGCCLISVAPRL*ASRCWGSFTSRPLIRSLAEKLTAGY 513
++G + GG AQ P K GC IS P S GS S I LA +LT
Sbjct: 3045 NSGASSLGGAVPRTRAQLMPRKNGCSFISFDPSYDPSLFEGSLCSSLYISCLAGRLTDIL 2866
Query: 514 SGKRSCWPMTLNSVARLLDPLKGVFPNSSSYKNTPKVHQSTALPWPSPLMISGARYSCVP 693
+G L +V L+ PLKGV P + SY+ P HQSTAL WP+P+MISGA YS VP
Sbjct: 2865 AGNLMSLCRMLANVLCLVLPLKGV*PYNISYRRIPSAHQSTALVWPAPVMISGAMYSSVP 2686
Query: 694 TNDID 708
TN++D
Sbjct: 2685 TNELD 2671
>gb|CN820184.1|CN820184 HRO4412_B04_D08ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4412_B04_D08, mRNA
sequence
Length = 610
Score = 155 bits (332), Expect = 1e-038
Identities = 59/109 (54%), Positives = 76/109 (69%)
Frame = -2 / +2
Query: 707 SMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGVFLYELLFGKTPFKGSSNRATLFNVIGQQ 528
S SFVGT EY+APEII G GH SAVDWW G+ LYE+L+G TPF+G + + T N++ +
Sbjct: 176 SNSFVGTEEYIAPEIITGAGHTSAVDWWALGILLYEMLYGYTPFRGKTRQRTFANILHKD 355
Query: 527 LRFPEYPAVSFSARDLIRGLLVKEPQQRLAYKRGATEIKQHPFFEGVNW 381
+RFP +VS AR LI LL ++P RL G+ EIK+HPFF G+NW
Sbjct: 356 IRFPASISVSLPARQLIYRLLHRDPSNRLGSHEGSNEIKEHPFFRGINW 502
Score = 105 bits (225), Expect = 6e-024
Identities = 53/114 (46%), Positives = 63/114 (55%)
Frame = +1 / -2
Query: 367 HLMSAQFTPSKKGCCLISVAPRL*ASRCWGSFTSRPLIRSLAEKLTAGYSGKRSCWPMTL 546
H Q P KKGC IS P S GS S I LA +LT +G L
Sbjct: 516 HAREPQLMPRKKGCSFISFDPSCDPSLFEGSLCSSLYISCLAGRLTDILAGNLMSLCRML 337
Query: 547 NSVARLLDPLKGVFPNSSSYKNTPKVHQSTALPWPSPLMISGARYSCVPTNDID 708
+V L+ PLKGV P + SY+ P HQSTAL WP+P+MISGA YS VPTN++D
Sbjct: 336 ANVLCLVLPLKGV*PYNISYRRIPSAHQSTALVWPAPVMISGAMYSSVPTNELD 175
>gb|CN819412.1|CN819412 HRO4405_G02_M03ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4405_G02_M03, mRNA
sequence
Length = 544
Score = 101 bits (216), Expect(3) = 2e-027
Identities = 38/42 (90%), Positives = 41/42 (97%)
Frame = -2 / +3
Query: 446 RLAYKRGATEIKQHPFFEGVNWALIRCASPPEVPKPVEIERP 321
RLAYKRGATEIKQHPFFEGVNWALIRCA+PP++PKPVEI RP
Sbjct: 66 RLAYKRGATEIKQHPFFEGVNWALIRCATPPDIPKPVEIPRP 191
Score = 26.7 bits (52), Expect(3) = 2e-027
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -2 / +2
Query: 509 PAVSFSARDLIRGLLVK 459
P F RDLIRGLL++
Sbjct: 5 PCRGFCCRDLIRGLLIR 55
Score = 26.3 bits (51), Expect(3) = 2e-027
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = -2 / +3
Query: 260 QKGVDGYLEFGFF* 219
+KG D YLEF FF*
Sbjct: 228 EKGPDNYLEFDFF* 269
>gb|CN817518.1|CN817518 HRO4454_B04_D08ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4454_B04_D08, mRNA sequence
Length = 473
Score = 95.5 bits (202), Expect = 9e-021
Identities = 35/81 (43%), Positives = 52/81 (64%)
Frame = -2 / +3
Query: 575 KGSSNRATLFNVIGQQLRFPEYPAVSFSARDLIRGLLVKEPQQRLAYKRGATEIKQHPFF 396
+G + + T +N++ + L FP VS A+ LI GLL ++P R+ GA +IK+HPFF
Sbjct: 6 RGKNRKKTFYNILHKDLTFPSSIPVSLEAKQLIHGLLQRDPSARIGSNTGANDIKEHPFF 185
Query: 395 EGVNWALIRCASPPEVPKPVE 333
E + W LIRC SPPE+ P++
Sbjct: 186 EDIYWPLIRCMSPPELDVPLK 248
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,841,692
Number of Sequences: 8143
Number of extensions: 132821
Number of successful extensions: 1432
Number of sequences better than 1.0e-020: 8
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 244684938
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)