TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2576612.2.1
(845 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN821219.1|CN821219 HRO4428_H01_P02ZS5 Lib AA069E1X Aven... 258 1e-069
gb|CN816185.1|CN816185 HRO4517_H07_O13ZS5 Lib AA071E1X Aven... 161 1e-040
gb|CN819635.1|CN819635 HRO4402_D11_G22ZS5 Lib AA069E1X Aven... 140 3e-034
>gb|CN821219.1|CN821219 HRO4428_H01_P02ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4428_H01_P02, mRNA
sequence
Length = 576
Score = 258 bits (557), Expect = 1e-069
Identities = 105/112 (93%), Positives = 111/112 (99%)
Frame = +3 / +3
Query: 300 FPARARRRFQRGLKRKPMALIKKLRKAKKDAPVGEKPEPVKTHLRNMIIVPEMIGSIVGV 479
FPARARRRF+RGLKRKPMAL+KKLRKAKKDAP GEKPEPV+THLRNMIIVPEMIGS++GV
Sbjct: 12 FPARARRRFKRGLKRKPMALVKKLRKAKKDAPAGEKPEPVRTHLRNMIIVPEMIGSLIGV 191
Query: 480 YNGKTFNQVEIKPEMIGHYLAEFSISYKPVKHGRPGIGATHSSRFIPLK*VV 635
YNGKTFNQVEIKPEMIGHYLAEFSISYKPVKHGRPGIGATHSSRFIPLK*V+
Sbjct: 192 YNGKTFNQVEIKPEMIGHYLAEFSISYKPVKHGRPGIGATHSSRFIPLK*VM 347
Score = 219 bits (473), Expect = 4e-058
Identities = 96/110 (87%), Positives = 98/110 (89%)
Frame = -2 / -3
Query: 631 THFRGINREE*VAPIPGLPCLTGL*EMENSAR*WPIISGLISTWLKVLPL*TPTMLPIIS 452
TH RG+NREE VAPIPGLPC TGL*EMENSAR*WPIISGLISTWLKVLPL*TP LPIIS
Sbjct: 343 THLRGMNREEWVAPIPGLPCFTGL*EMENSAR*WPIISGLISTWLKVLPL*TPIRLPIIS 164
Query: 451 GTMIMLRRWVFTGSGFSPTGASFFALRSFLMSAMGFLFKPLWNLLLARAG 302
GTMIMLRR V TGSGFSP GASFFALRSFL SAMGFL +PL NLL ARAG
Sbjct: 163 GTMIMLRRCVLTGSGFSPAGASFFALRSFLTSAMGFLLRPLLNLLRARAG 14
Score = 190 bits (409), Expect = 3e-049
Identities = 82/112 (73%), Positives = 92/112 (82%)
Frame = -1 / -2
Query: 635 NNSFQRDKPRGVSGTNTGPPVLDRLVGNGELCKIVANHLRLDLNLVEGLAIVDTNNAPNH 456
+NSF+RD+P GV GT+TGP +L RLVG+GEL K++ANHLRLDLNLVEGLAIVDT+ A NH
Sbjct: 347 HNSFERDEPGGVGGTDTGPTMLHRLVGDGELSKVMANHLRLDLNLVEGLAIVDTDKAANH 168
Query: 455 LGDNDHVAEMGLHWLWLLADRSILFRLAQLLDERHGLPLQTPLEPSPGARWE 300
L DNDHVAEM H L LL R ILFRLAQLLDERHGLPL+T LEPS G E
Sbjct: 167 LRDNDHVAEMCPHRLRLLTSRGILFRLAQLLDERHGLPLETSLEPSAGTGGE 12
Score = 147 bits (315), Expect = 3e-036
Identities = 64/111 (57%), Positives = 70/111 (63%)
Frame = +2 / +2
Query: 299 LPSARQEKVPEGFXXXXXXXXXXXXXXXKGCSCRREARASEDPSPQHDHCPRDDWEHCWC 478
+P +KV E KGC C EA A ED SPQHDHCP DDW+ C
Sbjct: 11 VPRPCPQKVQERSQEEAHGARQEAAQGEKGCPCW*EA*ACEDTSPQHDHCP*DDWQPYRC 190
Query: 479 LQWQDLQPG*DQA*DDWPLSCRVLHFLQAGQAREARYWCHSLLAVYPSEMS 631
LQWQDLQPG*DQA*DDWPL C VLH LQA +A ARY CH LL V+PS+MS
Sbjct: 191 LQWQDLQPG*DQA*DDWPLPC*VLHLLQACEAW*ARYRCHPLLPVHPSQMS 343
Score = 127 bits (273), Expect = 2e-030
Identities = 70/107 (65%), Positives = 70/107 (65%)
Frame = +1 / +1
Query: 313 PGEGSRGV*RGSPWRSSRSCARRKRMLLSARSQSQ*RPISAT*SLSPR*LGALLVSTMAR 492
P EGSR V RGSPWRSSRSCARRKRM L RS S * ISAT*SLS R*L AL VSTMAR
Sbjct: 25 PAEGSREVSRGSPWRSSRSCARRKRMPLLVRSLSL*GHISAT*SLSLR*LAALSVSTMAR 204
Query: 493 PXXXXXXXXXXXATILQSSPFPTSRSSTGGPVLVPLTPRGLSL*NEL 633
P A L SSP PTS S GPV VP TP G SL NEL
Sbjct: 205 PSTRLRSSLR*LAITLLSSPSPTSL*SMVGPVSVPPTPPGSSLSNEL 345
>gb|CN816185.1|CN816185 HRO4517_H07_O13ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4517_H07_O13, mRNA sequence
Length = 416
Score = 161 bits (347), Expect = 1e-040
Identities = 64/71 (90%), Positives = 69/71 (97%)
Frame = +3 / +2
Query: 417 VKTHLRNMIIVPEMIGSIVGVYNGKTFNQVEIKPEMIGHYLAEFSISYKPVKHGRPGIGA 596
V+THLRNMII+PEMIGS+VG+YNGK FN VEIKPEMIGHYLAEFS+SYKPVKHGRPGIGA
Sbjct: 11 VRTHLRNMIIMPEMIGSVVGIYNGKAFNSVEIKPEMIGHYLAEFSLSYKPVKHGRPGIGA 190
Query: 597 THSSRFIPLK* 629
THSSRFIPLK*
Sbjct: 191 THSSRFIPLK* 223
Score = 134 bits (287), Expect = 2e-032
Identities = 59/72 (81%), Positives = 61/72 (84%)
Frame = -2 / -3
Query: 631 THFRGINREE*VAPIPGLPCLTGL*EMENSAR*WPIISGLISTWLKVLPL*TPTMLPIIS 452
+H G+N EE VAPIPGLPCLTGL*E ENSAR*WPIISGLIST LK LPL* PT LPIIS
Sbjct: 225 SHLSGMNLEEWVAPIPGLPCLTGL*ERENSAR*WPIISGLISTELKALPL*IPTTLPIIS 46
Query: 451 GTMIMLRRWVFT 416
G MIMLRRWV T
Sbjct: 45 GMMIMLRRWVLT 10
Score = 112 bits (240), Expect = 6e-026
Identities = 47/71 (66%), Positives = 58/71 (81%)
Frame = -1 / -2
Query: 629 SFQRDKPRGVSGTNTGPPVLDRLVGNGELCKIVANHLRLDLNLVEGLAIVDTNNAPNHLG 450
S + ++P GV GTNT P VLD LVG GELC++VANHLRLDL+ VEGLA+VDT++A NHL
Sbjct: 223 SLEWNEP*GVGGTNTRPSVLDWLVGEGELCQVVANHLRLDLDGVEGLAVVDTDDASNHLR 44
Query: 449 DNDHVAEMGLH 417
+DHVAE+G H
Sbjct: 43 HDDHVAEVGPH 11
Score = 97.3 bits (206), Expect = 3e-021
Identities = 43/75 (57%), Positives = 49/75 (65%)
Frame = +2 / +1
Query: 419 EDPSPQHDHCPRDDWEHCWCLQWQDLQPG*DQA*DDWPLSCRVLHFLQAGQAREARYWCH 598
EDP PQHDH DDW+ LQ Q LQ DQA*DDWPL RVL LQA QAR+A YWCH
Sbjct: 13 EDPPPQHDHHA*DDWKRRRYLQRQGLQLRRDQA*DDWPLPGRVLPLLQASQARKAWYWCH 192
Query: 599 SLLAVYPSEMSCYPD 643
LL V+ +++ D
Sbjct: 193 PLLKVHSTQVRVVRD 237
>gb|CN819635.1|CN819635 HRO4402_D11_G22ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4402_D11_G22, mRNA
sequence
Length = 354
Score = 140 bits (300), Expect = 3e-034
Identities = 55/61 (90%), Positives = 59/61 (96%)
Frame = +3 / +3
Query: 447 VPEMIGSIVGVYNGKTFNQVEIKPEMIGHYLAEFSISYKPVKHGRPGIGATHSSRFIPLK 626
+PEMIGS+VG+YNGK FN VEIKPEMIGHYLAEFS+SYKPVKHGRPGIGATHSSRFIPLK
Sbjct: 6 MPEMIGSVVGIYNGKAFNSVEIKPEMIGHYLAEFSLSYKPVKHGRPGIGATHSSRFIPLK 185
Query: 627 * 629
*
Sbjct: 186 * 188
Score = 112 bits (240), Expect = 6e-026
Identities = 50/61 (81%), Positives = 52/61 (85%)
Frame = -2 / -3
Query: 631 THFRGINREE*VAPIPGLPCLTGL*EMENSAR*WPIISGLISTWLKVLPL*TPTMLPIIS 452
+H G+N EE VAPIPGLPCLTGL*E ENSAR*WPIISGLIST LK LPL* PT LPIIS
Sbjct: 190 SHLSGMNLEEWVAPIPGLPCLTGL*ERENSAR*WPIISGLISTELKALPL*IPTTLPIIS 11
Query: 451 G 449
G
Sbjct: 10 G 8
Score = 95.9 bits (203), Expect = 8e-021
Identities = 40/59 (67%), Positives = 49/59 (83%)
Frame = -1 / -2
Query: 629 SFQRDKPRGVSGTNTGPPVLDRLVGNGELCKIVANHLRLDLNLVEGLAIVDTNNAPNHL 453
S + ++P GV GTNT P VLD LVG GELC++VANHLRLDL+ VEGLA+VDT++A NHL
Sbjct: 188 SLEWNEP*GVGGTNTRPSVLDWLVGEGELCQVVANHLRLDLDGVEGLAVVDTDDASNHL 12
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,955,317
Number of Sequences: 8143
Number of extensions: 122191
Number of successful extensions: 895
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 289726444
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)