TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2521865.2.1
(638 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN817629.1|CN817629 HRO4463_B12_C23ZS5 Lib AA070E1X Aven... 277 1e-075
gb|CN816486.1|CN816486 HRO4521_C09_E17ZS5 Lib AA071E1X Aven... 238 5e-064
>gb|CN817629.1|CN817629 HRO4463_B12_C23ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4463_B12_C23, mRNA sequence
Length = 781
Score = 277 bits (600), Expect = 1e-075
Identities = 115/122 (94%), Positives = 117/122 (95%)
Frame = +2 / +2
Query: 2 LATGAHVVGNVLVHESAKIGEGCLIGPDVAIGPGCVVEDGVRLSRCTVMRGVRIKKHACI 181
LATG HVVGNVLVHESAKIGEGCLIGPDVAIGPGCVVEDGVRLSRCTVMRG RIKKHACI
Sbjct: 248 LATGTHVVGNVLVHESAKIGEGCLIGPDVAIGPGCVVEDGVRLSRCTVMRGARIKKHACI 427
Query: 182 SNSIIGWHSTVGQWARIENMTILGEDVHVCDEVYSNGGVVLPHKEIKSSILKPEIVM*AL 361
SNSI+GWHSTVG+WARIENMTILGEDVHV DEVYSNGGVVLPHKEIKSSILKPEIVM*A
Sbjct: 428 SNSIVGWHSTVGKWARIENMTILGEDVHVGDEVYSNGGVVLPHKEIKSSILKPEIVM*AH 607
Query: 362 QF 367
F
Sbjct: 608 HF 613
Score = 192 bits (413), Expect = 6e-050
Identities = 79/117 (67%), Positives = 95/117 (81%)
Frame = -2 / -1
Query: 358 SSHDDLRLQNA*LDLFMWENNTAIAVHLITHMNILPQDSHILYPCPLTNS*VPADNAV*D 179
SSHDDLRLQ+A*LDLF+W NNT IAV LIT+M IL QDSHI Y CP ++S VP++NAV*D
Sbjct: 604 SSHDDLRLQDA*LDLFVW*NNTTIAVDLITYMYILSQDSHIFYACPFSDSRVPSNNAV*D 425
Query: 178 ASMLLDTHAAHDSAAGKPHAVLHNTPRSNGDIRTNQTTFSNLGALMHQHIANNMCSS 8
ASMLL+T H +GKPHA+LHNT RS+ +IRTNQTT SN G L+HQ + +N+CSS
Sbjct: 424 ASMLLNTRTTHHRTSGKPHAILHNTSRSDSNIRTNQTTLSNFGTLVHQDVPDNVCSS 254
Score = 177 bits (380), Expect(2) = 1e-054
Identities = 72/83 (86%), Positives = 75/83 (90%)
Frame = -3 / -2
Query: 249 RIVIFSIRAH*PTVECQPIMLFEMQACFLIRTPRMTVQRESLTPSSTTHPGPMATSGPIR 70
RIVIFS+RAH PTVEC P MLFEMQACFLIR PR+TV RESLTPSSTTHPGP+ATSGPIR
Sbjct: 495 RIVIFSMRAHFPTVECHPTMLFEMQACFLIRAPRITVHRESLTPSSTTHPGPIATSGPIR 316
Query: 69 QPSPILALSCTSTLPTTCAPVAS 1
QPSPILALSCT T PTTC PVAS
Sbjct: 315 QPSPILALSCTKTFPTTCVPVAS 247
Score = 149 bits (319), Expect = 6e-037
Identities = 71/107 (66%), Positives = 76/107 (71%)
Frame = +3 / +3
Query: 9 LEHMLLAMCWCMRAPRLEKVV*LVLMSPLDLGVLWRTA*GFPAALSCAACVSRSMLASQT 188
LEH L WC R P+LE+VV*LVLM DL VLWR A*GFP C V RSMLASQT
Sbjct: 255 LEHTLSGTSWCTRVPKLERVV*LVLMLLSDLDVLWRMA*GFPDVR*CVVRVLRSMLASQT 434
Query: 189 ALSAGTQLLVNGHG*RI*LSWGRMFMCVMRCTAMAVLFSHIKRSSQA 329
AL GT+L NGH *+I*LSW RM+M VMR TAM VLF H KRSSQA
Sbjct: 435 ALLDGTRLSENGHA*KI*LSWERMYM*VMRSTAMVVLFYHTKRSSQA 575
Score = 128 bits (275), Expect(2) = 8e-044
Identities = 51/83 (61%), Positives = 62/83 (74%)
Frame = +1 / +1
Query: 1 ASYWSTCCWQCAGA*ERQDWRRLSDWS*CRHWTWVCCGGRREAFPLHCHARRAYQEACLH 180
A +W+T C + GA E Q+WR LSDWS*C + TW+CCGG REAFP++ A AY EACLH
Sbjct: 247 AGHWNTRCRERLGARECQNWRGLSDWS*CCYRTWMCCGGWREAFPMYGDAWCAY*EACLH 426
Query: 181 LKQHYRLALNCWSMGTDREYDYP 249
LKQH +AL+C MGT R+YDYP
Sbjct: 427 LKQHCWMALDCRKMGTHRKYDYP 495
Score = 113 bits (242), Expect(2) = 2e-033
Identities = 51/80 (63%), Positives = 56/80 (70%)
Frame = -1 / -3
Query: 248 G*SYSLSVPIDQQLSASR*CCLRCKHAS*YARRA*QCSGKASRRPPQHTQVQWRHQDQSD 69
G*SY L VPI +Q SA + CCLRCKHAS*YA A GKASR PPQH QV+ +HQDQSD
Sbjct: 494 G*SYFLCVPIFRQSSAIQQCCLRCKHAS*YAHHASPYIGKASRHPPQHIQVR*QHQDQSD 315
Query: 68 NLLQSWRSHAPAHCQQHVLQ 9
N LQ W S AP +Q V Q
Sbjct: 314 NPLQFWHSRAPRRSRQRVFQ 255
Score = 62.9 bits (131), Expect(2) = 8e-044
Identities = 27/37 (72%), Positives = 28/37 (75%)
Frame = +1 / +1
Query: 283 QQWRCCSPT*RDQVKHSEA*DRHVSSPVLATVHS*LL 393
QQW CCS T RDQVKH EA*DRHVSSP+L V LL
Sbjct: 529 QQWWCCSTTQRDQVKHPEA*DRHVSSPLLVMVDQCLL 639
Score = 51.0 bits (105), Expect(2) = 1e-054
Identities = 20/21 (95%), Positives = 20/21 (95%)
Frame = -3 / -2
Query: 363 WRAHMTISGFRMLDLISLCGR 301
W AHMTISGFRMLDLISLCGR
Sbjct: 609 W*AHMTISGFRMLDLISLCGR 547
Score = 43.2 bits (88), Expect(2) = 2e-033
Identities = 20/27 (74%), Positives = 23/27 (85%)
Frame = -1 / -3
Query: 377 TVAKTGELT*RSQASECLT*SLYVGEQ 297
T+ ++GELT*RSQAS CLT*SL V EQ
Sbjct: 623 TITRSGELT*RSQASGCLT*SLCVVEQ 543
>gb|CN816486.1|CN816486 HRO4521_C09_E17ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4521_C09_E17, mRNA sequence
Length = 431
Score = 238 bits (515), Expect = 5e-064
Identities = 98/104 (94%), Positives = 100/104 (96%)
Frame = +2 / +2
Query: 56 IGEGCLIGPDVAIGPGCVVEDGVRLSRCTVMRGVRIKKHACISNSIIGWHSTVGQWARIE 235
IGEGCLIGPDVAIGPGCVVEDGVRLSRCTVMRG RIKKHACISNSI+GWHSTVG+WARIE
Sbjct: 2 IGEGCLIGPDVAIGPGCVVEDGVRLSRCTVMRGARIKKHACISNSIVGWHSTVGKWARIE 181
Query: 236 NMTILGEDVHVCDEVYSNGGVVLPHKEIKSSILKPEIVM*ALQF 367
NMTILGEDVHV DEVYSNGGVVLPHKEIKSSILKPEIVM*A F
Sbjct: 182 NMTILGEDVHVGDEVYSNGGVVLPHKEIKSSILKPEIVM*AHHF 313
Score = 168 bits (361), Expect = 9e-043
Identities = 71/101 (70%), Positives = 82/101 (81%)
Frame = -2 / -2
Query: 358 SSHDDLRLQNA*LDLFMWENNTAIAVHLITHMNILPQDSHILYPCPLTNS*VPADNAV*D 179
SSHDDL LQ+A*LDLF+W NNT IAV LIT+M IL QDSHI Y CP +NS VP++NAV*D
Sbjct: 304 SSHDDLGLQDA*LDLFVW*NNTTIAVDLITYMYILSQDSHIFYACPFSNSRVPSNNAV*D 125
Query: 178 ASMLLDTHAAHDSAAGKPHAVLHNTPRSNGDIRTNQTTFSN 56
ASMLL+T A H +GKPH +LHNT RS+ +IRTNQTT SN
Sbjct: 124 ASMLLNTRATHHRTSGKPHTILHNTSRSDSNIRTNQTTLSN 2
Score = 140 bits (300), Expect(2) = 1e-043
Identities = 57/65 (87%), Positives = 60/65 (92%)
Frame = -3 / -3
Query: 249 RIVIFSIRAH*PTVECQPIMLFEMQACFLIRTPRMTVQRESLTPSSTTHPGPMATSGPIR 70
RIVIFS+RAH PTVEC P MLFEMQACFLIR PR+TV RESLTPSSTTHPGP+ATSGPIR
Sbjct: 195 RIVIFSMRAHFPTVECHPTMLFEMQACFLIRAPRITVHRESLTPSSTTHPGPIATSGPIR 16
Query: 69 QPSPI 55
QPSPI
Sbjct: 15 QPSPI 1
Score = 129 bits (276), Expect = 5e-031
Identities = 64/91 (70%), Positives = 68/91 (74%)
Frame = +3 / +3
Query: 57 LEKVV*LVLMSPLDLGVLWRTA*GFPAALSCAACVSRSMLASQTALSAGTQLLVNGHG*R 236
LE+VV*LVLM DL VLWR *GFP C A V RSMLASQTAL GT+LL NGH *+
Sbjct: 3 LERVV*LVLMLLSDLDVLWRMV*GFPDVR*CVARVLRSMLASQTALLDGTRLLENGHA*K 182
Query: 237 I*LSWGRMFMCVMRCTAMAVLFSHIKRSSQA 329
I*LSW RM+M VMR TAM VLF H KRSSQA
Sbjct: 183 I*LSWERMYM*VMRSTAMVVLFYHTKRSSQA 275
Score = 118 bits (252), Expect(2) = 6e-040
Identities = 44/65 (67%), Positives = 52/65 (80%)
Frame = +1 / +1
Query: 55 DWRRLSDWS*CRHWTWVCCGGRREAFPLHCHARRAYQEACLHLKQHYRLALNCWSMGTDR 234
+WR LSDWS*C + TW+CCGG EAFP++ A RAY EACLHLKQH +AL+CW MGT R
Sbjct: 1 NWRGLSDWS*CCYRTWMCCGGWCEAFPMYGDAWRAY*EACLHLKQHCWMALDCWKMGTHR 180
Query: 235 EYDYP 249
+YDYP
Sbjct: 181 KYDYP 195
Score = 98.7 bits (209), Expect(2) = 2e-028
Identities = 45/64 (70%), Positives = 48/64 (75%)
Frame = -1 / -1
Query: 248 G*SYSLSVPIDQQLSASR*CCLRCKHAS*YARRA*QCSGKASRRPPQHTQVQWRHQDQSD 69
G*SY L VPI QQ SA + CCLRCKHAS*YAR A GKAS PPQH QV+ +HQDQSD
Sbjct: 194 G*SYFLCVPIFQQSSAIQQCCLRCKHAS*YARHASPYIGKASHHPPQHIQVR*QHQDQSD 15
Query: 68 NLLQ 57
N LQ
Sbjct: 14 NPLQ 3
Score = 60.6 bits (126), Expect(2) = 6e-040
Identities = 26/37 (70%), Positives = 27/37 (72%)
Frame = +1 / +1
Query: 283 QQWRCCSPT*RDQVKHSEA*DRHVSSPVLATVHS*LL 393
QQW CCS T RDQVKH EA DRHVSSP+L V LL
Sbjct: 229 QQWWCCSTTQRDQVKHPEARDRHVSSPLLVMVDQCLL 339
Score = 51.0 bits (105), Expect(2) = 1e-043
Identities = 20/21 (95%), Positives = 20/21 (95%)
Frame = -3 / -3
Query: 363 WRAHMTISGFRMLDLISLCGR 301
W AHMTISGFRMLDLISLCGR
Sbjct: 309 W*AHMTISGFRMLDLISLCGR 247
Score = 41.4 bits (84), Expect(2) = 2e-028
Identities = 19/27 (70%), Positives = 23/27 (85%)
Frame = -1 / -1
Query: 377 TVAKTGELT*RSQASECLT*SLYVGEQ 297
T+ ++GELT*RS+AS CLT*SL V EQ
Sbjct: 323 TITRSGELT*RSRASGCLT*SLCVVEQ 243
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,660,375
Number of Sequences: 8143
Number of extensions: 113256
Number of successful extensions: 2284
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 208331770
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)