TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2521334.2.1
(1277 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN820263.1|CN820263 HRO4417_D05_G09ZS5 Lib AA069E1X Aven... 292 1e-079
gb|CN820072.1|CN820072 HRO4410_G07_M14ZS5 Lib AA069E1X Aven... 110 7e-037
gb|CN818277.1|CN818277 HRO4470_C12_F24ZS5 Lib AA070E1X Aven... 110 5e-025
>gb|CN820263.1|CN820263 HRO4417_D05_G09ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4417_D05_G09, mRNA
sequence
Length = 592
Score = 292 bits (631), Expect = 1e-079
Identities = 124/163 (76%), Positives = 138/163 (84%)
Frame = +1 / +1
Query: 550 YRYDGPEVNTRLAHSEAKLLHEKIHHKAYSDDEIIRILTTRSKPQLIATFNHYNDAFGHR 729
YRY PEVNT +AHSEAKLLH KI KAY D+EIIRILTTRSK QL+ATFN YND F H
Sbjct: 7 YRYFRPEVNTSVAHSEAKLLHGKIQEKAYGDEEIIRILTTRSKAQLLATFNSYNDQFSHP 186
Query: 730 INKDLKADPQDEYLRTLRAIIRCFSCPDRYFEKVARQAIAGLGTDENSLTRVITTRAEVD 909
I KDLK DP+DE+L TLRAIIRCF+CPDRYFEK+ R A+ G+GTDENSLTR+ITTRAEVD
Sbjct: 187 ITKDLKEDPKDEFLATLRAIIRCFACPDRYFEKIIRLALGGVGTDENSLTRIITTRAEVD 366
Query: 910 LKLIKEAYQKRNSVPLERAVAGDTSGDYESMLLALLGQE**AV 1038
LKLIKEAY KRNSVPLE+AV+ DT+ DYE M+LALLG E * V
Sbjct: 367 LKLIKEAYLKRNSVPLEKAVSKDTTRDYEDMMLALLGAEY*GV 495
Score = 172 bits (370), Expect = 1e-043
Identities = 79/163 (48%), Positives = 105/163 (64%)
Frame = -3 / -2
Query: 1038 YGSSLLPQEREEHALVVAGGVPRDGPLQRHAVPLLVRLLNQFEVHLSAGGDDPGQGVLIC 859
Y S L ++ + H +V+G V + LQR V L V LL+Q +VHL A G DP +GVL+C
Sbjct: 495 YASVLCSEKCKHHVFIVSGRVLGNSFLQRDTVSLQVGLLDQLQVHLGASGYDPCKGVLVC 316
Query: 858 A*PRYGLPSHLLKVPVRATEAPDDRPQRSEVLVLGVSLQILVDSVAKCIIVMIECSNQLR 679
A P LLKV VRA EAP DRPQ + L+LG+ LQ+L D + + IIV +EC L
Sbjct: 315 ADTSKSQPDDLLKVSVRAGEAPYDRPQGRQELILGILLQVLGDGMTELIIVTVECCK*LS 136
Query: 678 FAPSGEDSDDLIITVSLVMDLLMK*FGF*VCQPCVDLWSIIAV 550
FAP E+ D+ ++T+SL++DL M+*FG VC CV LW+ IAV
Sbjct: 135 FAPCSENPDNFLVTISLLLDLTME*FGLRVCH*CVHLWAEIAV 7
Score = 155 bits (333), Expect(2) = 7e-041
Identities = 89/140 (63%), Positives = 96/140 (68%)
Frame = -1 / -3
Query: 1079 QTPRRRSERHQRTSTAHHSCPRSARSMLS*SPEVSPATARSSGTLFLFWYASLISLRSTS 900
QT R + + T +S PRSA M S*S VS TA SSGTLFLF ASLIS RSTS
Sbjct: 536 QTRRATT*GSRSDHTPQYSAPRSASIMSS*SLVVSLETAFSSGTLFLFR*ASLISFRSTS 357
Query: 899 ARVVMTLVREFSSVPSPAMACLATFSKYLSGQLKHRMIARSVLRYSSWGSAFRSLLIRWP 720
ARVVM LVREFSSVP+P A FSKYLSGQ KH MIA V R SS GS+FRSL++
Sbjct: 356 ARVVMILVREFSSVPTPPRANRMIFSKYLSGQAKHLMIALRVARNSSLGSSFRSLVMG*L 177
Query: 719 NASL**LNVAIS*GLLLVVR 660
N SL* LNVA S* LLLVVR
Sbjct: 176 N*SL*LLNVASS*ALLLVVR 117
Score = 115 bits (245), Expect = 2e-026
Identities = 59/129 (45%), Positives = 76/129 (58%)
Frame = +3 / +3
Query: 549 LPL*WTRGQHKVGTLRSQTTS*EDPSQGLQ***DHQNPHH*EQTSADCYIQSLQ*CIWPP 728
+PL RG+H GTL Q T DP +GL * ++Q+ H+ EQ+SA C IQ LQ* +
Sbjct: 6 IPLFPPRGEHISGTL*GQITPW*DPGEGLW*RGNYQDSHYKEQSSATCNIQQLQ*SVQSS 185
Query: 729 NQQGSEG*PPGRVPQNAAGDHPVLQLP*QVL*EXXXXXXXXXXXXXELPDQGHHHPR*GG 908
+ QG EG G VP + GDH VL+LP*Q+L*E ELP + H+H R GG
Sbjct: 186 HHQGPEGGSQG*VPGDPEGDHKVLRLP*QIL*EDHPVGSWRCRHRRELPYKDHNHSRRGG 365
Query: 909 PQTD*GGVP 935
P+ D GG+P
Sbjct: 366 PEADQGGLP 392
Score = 27.6 bits (54), Expect(2) = 7e-041
Identities = 13/19 (68%), Positives = 14/19 (73%)
Frame = -1 / -3
Query: 608 SSLASECANLVLTSGPS*R 552
S+LASECA V TSG *R
Sbjct: 65 SNLASECATDVFTSGRK*R 9
>gb|CN820072.1|CN820072 HRO4410_G07_M14ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4410_G07_M14, mRNA
sequence
Length = 798
Score = 110 bits (235), Expect(2) = 7e-037
Identities = 49/126 (38%), Positives = 74/126 (58%)
Frame = +1 / +2
Query: 652 IRILTTRSKPQLIATFNHYNDAFGHRINKDLKADPQDEYLRTLRAIIRCFSCPDRYFEKV 831
IRI T RS + A + Y+ F + K +K++ + L I+RC P +YF KV
Sbjct: 218 IRIFTERSWAHMAAVASAYHHMFDRSLVKVVKSETSGNFEVALVTILRCAENPAKYFAKV 397
Query: 832 ARQAIAGLGTDENSLTRVITTRAEVDLKLIKEAYQKRNSVPLERAVAGDTSGDYESMLLA 1011
R+++ GLGTD+ +L RV+ TR E+D++ IK Y K+ PL A+ +TSG Y + LL+
Sbjct: 398 LRKSMKGLGTDDKTLVRVVVTRTEIDMQYIKAEYYKKYKKPLADAIHSETSGGYRTFLLS 577
Query: 1012 LLGQE* 1029
L+G *
Sbjct: 578 LVGSH* 595
Score = 59.3 bits (123), Expect(2) = 7e-037
Identities = 25/70 (35%), Positives = 42/70 (60%)
Frame = +1 / +2
Query: 439 FATRQAYHERFKRSLEEDIAAHVTGDFRKLLVPLVSTYRYDGPEVNTRLAHSEAKLLHEK 618
F TRQ Y+ +F LE DI+ +GD +K+L+ + RY+GPEV+ + +AK L++
Sbjct: 2 FGTRQTYYSKFGTYLEHDISYQTSGDHQKILLAYLGIPRYEGPEVDPTIVTHDAKDLYKA 181
Query: 619 IHHKAYSDDE 648
K +D++
Sbjct: 182 GEKKFGTDEK 211
>gb|CN818277.1|CN818277 HRO4470_C12_F24ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4470_C12_F24, mRNA sequence
Length = 620
Score = 110 bits (235), Expect = 5e-025
Identities = 49/126 (38%), Positives = 74/126 (58%)
Frame = +1 / +1
Query: 652 IRILTTRSKPQLIATFNHYNDAFGHRINKDLKADPQDEYLRTLRAIIRCFSCPDRYFEKV 831
IRI T RS + A + Y+ F + K +K++ + L I+RC P +YF KV
Sbjct: 112 IRIFTERSWAHMAAVASAYHHMFDRSLVKVVKSETSGNFEVALVTILRCAENPAKYFAKV 291
Query: 832 ARQAIAGLGTDENSLTRVITTRAEVDLKLIKEAYQKRNSVPLERAVAGDTSGDYESMLLA 1011
R+++ GLGTD+ +L RV+ TR E+D++ IK Y K+ PL A+ +TSG Y + LL+
Sbjct: 292 LRKSMKGLGTDDKTLVRVVVTRTEIDMQYIKAEYYKKYKKPLADAIHSETSGGYRTFLLS 471
Query: 1012 LLGQE* 1029
L+G *
Sbjct: 472 LVGSH* 489
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 12,973,532
Number of Sequences: 8143
Number of extensions: 175432
Number of successful extensions: 1075
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 460703295
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)