TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2493944.2.1
(2055 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816489.1|CN816489 HRO4521_C12_E23ZS5 Lib AA071E1X Aven... 168 3e-076
emb|X73601.1|PSBFRTSID A.sativa mRNA for invertase (beta-fr... 82 1e-036
gb|CN816688.1|CN816688 HRO4523_F05_L09ZS5 Lib AA071E1X Aven... 130 9e-031
>gb|CN816489.1|CN816489 HRO4521_C12_E23ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4521_C12_E23, mRNA sequence
Length = 874
Score = 168 bits (362), Expect(5) = 3e-076
Identities = 61/109 (55%), Positives = 81/109 (74%)
Frame = -1 / +1
Query: 1053 LRYDYGNFYASKTFYDPAKRRRILWGWANESDTAADDVAKGWAGIQAIPRTVWLDPSGKQ 874
L+ DYGN+YASK+F+D RRI+WGW NESD+++DDVAKGWAGI AIPR +WLD KQ
Sbjct: 133 LKIDYGNYYASKSFFDSKNSRRIIWGWTNESDSSSDDVAKGWAGIYAIPRAIWLDSDAKQ 312
Query: 873 LLQWPIEEVEALRAKSVTLRNRVIKAGHHVEVTGIQTAQADVEVSFEVS 727
LLQWP++E+E+LR + + + G E+ GI T QADVE+ FE++
Sbjct: 313 LLQWPVDEIESLRGNEINHQGLELNKGDLFEIKGIDTTQADVEIDFELT 459
Score = 71.2 bits (149), Expect(4) = 4e-028
Identities = 35/65 (53%), Positives = 41/65 (63%)
Frame = +2 / -3
Query: 845 STSSIGHCSSCFPLGSSHTVLGIAWIPAQPLATSSAAVSDSLAQPHRMRRRLAGS*NVLD 1024
S SS GHCS+CF S+ LGIA +PAQP ATSS +SDSL QPH + S N +
Sbjct: 341 SISSTGHCSNCFASLSNQIALGIA*MPAQPFATSSEELSDSLVQPHMILLLFFESKNDFE 162
Query: 1025 A*KLP 1039
A* LP
Sbjct: 161 A**LP 147
Score = 63.4 bits (132), Expect(5) = 3e-076
Identities = 28/65 (43%), Positives = 38/65 (58%)
Frame = -1 / +1
Query: 747 EVSFEVSPAALAGAETLDPALAYDAERLCGVKRADVRGGVGPFGLWVLASANRKERTAVF 568
+V + + AE DP+ DAE+ C A V GGVGPFGL +LAS + +E T +
Sbjct: 433 DVEIDFELTSTDDAEPFDPSWLLDAEKHCREAGASVHGGVGPFGLVILASDDMEEHTVLH 612
Query: 567 FRVFK 553
FRV+K
Sbjct: 613 FRVYK 627
Score = 59.3 bits (123), Expect(5) = 3e-076
Identities = 23/30 (76%), Positives = 27/30 (90%)
Frame = -1 / +1
Query: 432 KISLRSLIDRSVVESFGAGGKTCILSRVYP 343
KISLR+LIDRS VESFG GG+ CI++RVYP
Sbjct: 745 KISLRTLIDRSAVESFGGGGRLCIIARVYP 834
Score = 52.8 bits (109), Expect(4) = 4e-028
Identities = 23/32 (71%), Positives = 26/32 (81%)
Frame = +2 / -3
Query: 335 ASDG*TLERMQVLPPAPKLSTTDRSIRLLRDI 430
++ G*TL MQ LPP PKLST DRSIR+LRDI
Sbjct: 842 SATG*TLAMMQSLPPPPKLSTADRSIRVLRDI 747
Score = 42.8 bits (87), Expect(5) = 3e-076
Identities = 15/32 (46%), Positives = 22/32 (68%)
Frame = -1 / +1
Query: 537 KPVVLMCTDPTKSSLNPNLYRPTFAGFVDTDI 442
K ++LMC+D +SSL P LY P + GF + D+
Sbjct: 637 KYMILMCSDLRRSSLRPELYTPAYGGFFEFDL 732
Score = 35.9 bits (72), Expect(4) = 4e-028
Identities = 17/30 (56%), Positives = 18/30 (60%)
Frame = +2 / -3
Query: 560 TLKNTAVLSLRLAEASTHSPNGPTPPLTSA 649
TLK V S +EA SPNGPTPP T A
Sbjct: 620 TLKCRTVCSSMSSEARITSPNGPTPP*TDA 531
Score = 25.4 bits (49), Expect(4) = 4e-028
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = +2 / -3
Query: 731 TSKLTSTSACAVCIPVTST*CP 796
+SK STSAC V +P+ S P
Sbjct: 455 SSKSISTSACVVSMPLISKRSP 390
Score = 23.1 bits (44), Expect(5) = 3e-076
Identities = 8/29 (27%), Positives = 15/29 (51%)
Frame = -1 / +1
Query: 1149 LDLRRYDYYTVGTYHPRAERYVPDDPAGD 1063
+ L + D Y +G Y + + ++PD D
Sbjct: 34 VSLDQCDKYMIGVYDLKRDAFLPDTVLDD 120
>emb|X73601.1|PSBFRTSID A.sativa mRNA for invertase (beta-fructofuranosidase) (partial)
Length = 511
Score = 82.2 bits (173), Expect(4) = 1e-036
Identities = 31/54 (57%), Positives = 38/54 (70%)
Frame = -1 / +2
Query: 1731 GNIVWAHSVSRDLINWVALEPALRPSIPGDRYGCWSGSATVLPDGGGPVIMYTG 1570
G+IVW H+VS DL+NW+ LEPA+ PGD GCW+GSAT+LP G VI G
Sbjct: 71 GDIVWGHAVSTDLVNWIILEPAIERDSPGDINGCWTGSATILPGGQPIVIYTGG 232
Score = 51.0 bits (105), Expect(4) = 1e-036
Identities = 18/30 (60%), Positives = 23/30 (76%)
Frame = -1 / +2
Query: 1551 NYQVRNVAYPKNVSDPLLREWVKPSHNPVI 1462
N+QV+N+ PKN SDP LREW K +NPV+
Sbjct: 242 NHQVQNIMLPKNRSDPYLREWTKAGNNPVL 331
Score = 36.8 bits (74), Expect(4) = 1e-036
Identities = 13/19 (68%), Positives = 13/19 (68%)
Frame = -1 / +1
Query: 1794 PMYYKGWYHFFYQYNPKGA 1738
P Y G YH FYQYNP GA
Sbjct: 10 PCTYNGIYHSFYQYNPHGA 66
Score = 35.4 bits (71), Expect(4) = 1e-036
Identities = 12/17 (70%), Positives = 13/17 (76%)
Frame = -1 / +2
Query: 1449 GINVTQFRDPTTAWRGP 1399
G N +QFRDPTT W GP
Sbjct: 347 GFNSSQFRDPTTGWIGP 397
>gb|CN816688.1|CN816688 HRO4523_F05_L09ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4523_F05_L09, mRNA sequence
Length = 632
Score = 130 bits (278), Expect = 9e-031
Identities = 53/62 (85%), Positives = 58/62 (93%)
Frame = -1 / +1
Query: 411 IDRSVVESFGAGGKTCILSRVYPSLAIGKDARLYVFNNGRAHVKVSRLTAWEMKKPVMNG 232
IDRSVVESFGAGGKTCILSRVYPS+A+GKDA L+VFNNG +KVSRLTAWEMKKP+MNG
Sbjct: 4 IDRSVVESFGAGGKTCILSRVYPSMALGKDAHLHVFNNGATDIKVSRLTAWEMKKPLMNG 183
Query: 231 A* 226
A*
Sbjct: 184 A* 189
Score = 109 bits (232), Expect = 2e-024
Identities = 48/61 (78%), Positives = 50/61 (81%)
Frame = +2 / -1
Query: 230 APFMTGFFISHAVRRDTLTCALPLLNT*RRASLPMASDG*TLERMQVLPPAPKLSTTDRS 409
APF+ GFFISHAVRRDTL PLLNT*R AS P A DG TL+RMQV PPAPKLSTTDRS
Sbjct: 185 APFINGFFISHAVRRDTLISVAPLLNT*RCASFPNAMDGYTLDRMQVFPPAPKLSTTDRS 6
Query: 410 I 412
I
Sbjct: 5 I 3
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 18,772,841
Number of Sequences: 8143
Number of extensions: 260411
Number of successful extensions: 1620
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 45
effective length of database: 1,201,052
effective search space used: 767472228
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)