TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2448655.2.1
(682 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815304.1|CN815304 HRO4508_B12_D24ZS5 Lib AA071E1X Aven... 176 4e-045
gb|CN820984.1|CN820984 HRO4422_B12_C24ZS5 Lib AA069E1X Aven... 117 1e-027
>gb|CN815304.1|CN815304 HRO4508_B12_D24ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4508_B12_D24, mRNA sequence
Length = 556
Score = 176 bits (378), Expect = 4e-045
Identities = 68/80 (85%), Positives = 72/80 (90%)
Frame = +2 / +1
Query: 197 PTKYIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYLYGTDKGYRFHKTYLAKLKDLGH 376
P KYIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYLYGTD+GYRFHK YLAKLKDL
Sbjct: 10 PDKYIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYLYGTDRGYRFHKAYLAKLKDLAP 189
Query: 377 NDGEKGDGSGPSYVKLD*IA 436
+DGEK D +G SY KLD*+A
Sbjct: 190 SDGEKEDANGFSYTKLD*MA 249
Score = 116 bits (247), Expect = 5e-027
Identities = 49/76 (64%), Positives = 53/76 (69%)
Frame = -3 / -1
Query: 431 SNPVSHSWVRCRLLSRHHYAPDPSALLGMFCGICNLCQCHISNHSK*KQKQNCFGSGLLH 252
SNP S S + LLS H P PS LLG GICNLCQ I NHS *K+KQNC G GL H
Sbjct: 244 SNPTSCS*IHWHLLSHHRLEPGPSTLLGKPYGICNLCQSRIGNHST*KRKQNCSGFGLQH 65
Query: 251 NDDSHGILLLHRMEYT 204
+DD+HGILLLHR EYT
Sbjct: 64 SDDNHGILLLHRTEYT 17
Score = 107 bits (229), Expect = 2e-024
Identities = 47/53 (88%), Positives = 48/53 (90%)
Frame = -2 / -3
Query: 354 ARYVLWNL*PLSVPYK*SQ*VKTEAKLLWLWPPT****SWYSAPP*NGIYFVG 196
AR LWNL*PLSVPY+*SQ VKTEAKLLWLWPPT****SWYSAPP*NGIY G
Sbjct: 167 AR*ALWNL*PLSVPYR*SQYVKTEAKLLWLWPPT****SWYSAPP*NGIYLSG 9
Score = 107 bits (229), Expect = 2e-024
Identities = 52/79 (65%), Positives = 60/79 (75%)
Frame = -1 / -2
Query: 433 NLIQFHIAGSAAVSFLAIIMPQILQLC*VCFVESVTFVSAI*VITVSENRSKIALALASY 254
+LIQ +A S + FL I Q+LQLC*V +ESVT VS +*VITV EN SKIALALAS
Sbjct: 246 HLIQLRVAESIGIFFLTIAWSQVLQLC*VSLMESVTSVSPV*VITVRENGSKIALALASN 67
Query: 253 IMMIVMVFCSSIEWNILCR 197
I+MI+MVFCSSIE NIL R
Sbjct: 66 IVMIIMVFCSSIERNILVR 10
Score = 99.6 bits (211), Expect = 5e-022
Identities = 49/76 (64%), Positives = 55/76 (72%)
Frame = +3 / +2
Query: 204 SIFHSMEEQNTMTIIIM*EARAKAILLLFSLTVITYMALTKVTDSTKHT*QS*RIWGIMM 383
SIF SMEEQNTM II M EA+A+AILL FS TVITY LT+VTDS + T*QS*R W M
Sbjct: 17 SIFRSMEEQNTMIIITMLEAKARAILLPFSRTVITYTGLTEVTDSIRLT*QS*RTWLQAM 196
Query: 384 ARKETAADPAM*NWIR 431
RK+ D A +WIR
Sbjct: 197 VRKKMPMDSATRSWIR 244
>gb|CN820984.1|CN820984 HRO4422_B12_C24ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4422_B12_C24, mRNA
sequence
Length = 551
Score = 117 bits (251), Expect = 1e-027
Identities = 43/57 (75%), Positives = 50/57 (87%)
Frame = +2 / +1
Query: 200 TKYIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYLYGTDKGYRFHKTYLAKLKDL 370
TK IPFYGGAE+HDYHH VG +SQSNF+SVFT+CDYLYGTDKGY+ HK L KLK++
Sbjct: 13 TKLIPFYGGAEHHDYHHRVGQKSQSNFSSVFTFCDYLYGTDKGYKLHKASLTKLKEM 183
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,524,525
Number of Sequences: 8143
Number of extensions: 132672
Number of successful extensions: 1379
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 225488504
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)