TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2448385.2.9
(708 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815814.1|CN815814 HRO4510_G07_M14ZS5 Lib AA071E1X Aven... 227 5e-067
gb|CN815334.1|CN815334 HRO4508_E07_J14ZS5 Lib AA071E1X Aven... 212 4e-056
gb|CN816419.1|CN816419 HRO4520_E07_J14ZS5 Lib AA071E1X Aven... 85 1e-035
>gb|CN815814.1|CN815814 HRO4510_G07_M14ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4510_G07_M14, mRNA sequence
Length = 522
Score = 227 bits (490), Expect(2) = 5e-067
Identities = 95/108 (87%), Positives = 101/108 (93%)
Frame = +1 / +2
Query: 211 PFAGSSHAKGIVLEKIGIEAKQPNSAIRKCARVQLVKNGKKIAAFVPNDGCLNYIEENDE 390
PF GSSHAKGIVLEKIGIEAKQPNSAIRKC RVQL+KNGKKI AFVPNDGCL +IEENDE
Sbjct: 113 PFQGSSHAKGIVLEKIGIEAKQPNSAIRKCVRVQLIKNGKKITAFVPNDGCLLFIEENDE 292
Query: 391 VLIAGFGRKGHAVGDIPGVRFKVVKVSGVSLLALFKEKKEKPRS*ITF 534
VL++GFGR GHAVGDIPGVRFKVVKV+GVSLLAL+ EKKEKPRS*I F
Sbjct: 293 VLVSGFGRSGHAVGDIPGVRFKVVKVAGVSLLALYTEKKEKPRS*ICF 436
Score = 141 bits (302), Expect(2) = 2e-036
Identities = 67/109 (61%), Positives = 74/109 (67%)
Frame = -2 / -1
Query: 533 KVI*DLGFSFFSLKSASSXXXXXXXXXXXXPGMSPTA*PLRPNPAINTSSFSSM*FRQPS 354
K I DLGFSFFS+ AS PG+SPTA*P P P TSSFSSM +QPS
Sbjct: 435 KQIYDLGFSFFSV*RASKETPATLTTLKRTPGISPTA*PDLPKPETKTSSFSSMNNKQPS 256
Query: 353 FGTKAAIFFPFFTS*TRAHLRMAEFGCLASIPIFSRTMPLA*DDPAKGF 207
FGTKA IF PF +*T HLRMAE GCLASIPIFSRT+PLA ++P GF
Sbjct: 255 FGTKAVIFLPFLIN*TLTHLRMAELGCLASIPIFSRTIPLAWEEPWNGF 109
Score = 96.8 bits (205), Expect = 3e-021
Identities = 41/102 (40%), Positives = 69/102 (67%)
Frame = -1 / -3
Query: 534 ESDLRPWXXXXXLEECKQRHTRHLNNLEPDTRNVSHSMTLTTKSSNQHLIILLDVV*ATI 355
+++L W + +Q T +L+NLE D RN+++S+T +T++ NQ+ ++ D AT+
Sbjct: 436 KTNL*SWLFLLFCVKSEQGDTSNLDNLETDARNITNSVTGSTETRNQNFVVFFDEQQATV 257
Query: 354 IRHKGSNLLSILHQLNTGTLTDGRIWLLGLNTNLLQNDALGV 229
I ++ SNLL++L QLN+ TLT+GR+ LL NT+L +ND L +
Sbjct: 256 IWNESSNLLTVLDQLNSDTLTNGRVRLLSFNTDLFKNDTLSM 131
Score = 42.3 bits (86), Expect(2) = 5e-067
Identities = 17/35 (48%), Positives = 23/35 (65%)
Frame = +1 / +2
Query: 103 KTRGMGAGRKLKTHRRNQRWADKAYKKSHLGNEWK 207
K +G+ + RKL+T RR +WADK +KK LG K
Sbjct: 2 KPKGIKSARKLQTIRRASKWADKQWKKKALGTALK 106
Score = 25.8 bits (50), Expect(2) = 2e-036
Identities = 14/32 (43%), Positives = 17/32 (53%)
Frame = -2 / -1
Query: 206 FHSLPKWLFLYALSAHLWFLRWVLSLRPAPIP 111
F ++PK F + LSAHL L V S IP
Sbjct: 105 FKAVPKAFFFHCLSAHLDALLIVWSFLADLIP 10
>gb|CN815334.1|CN815334 HRO4508_E07_J14ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4508_E07_J14, mRNA sequence
Length = 473
Score = 212 bits (458), Expect = 4e-056
Identities = 86/112 (76%), Positives = 98/112 (87%)
Frame = +1 / -1
Query: 190 LGNEWKKPFAGSSHAKGIVLEKIGIEAKQPNSAIRKCARVQLVKNGKKIAAFVPNDGCLN 369
LG+ +K PF G+SHAKGIV EKIG+EAKQPNSAIRKC RV L KNGKKIAAFVP DGCLN
Sbjct: 473 LGSRYKNPFMGASHAKGIVTEKIGVEAKQPNSAIRKCVRVLLKKNGKKIAAFVPRDGCLN 294
Query: 370 YIEENDEVLIAGFGRKGHAVGDIPGVRFKVVKVSGVSLLALFKEKKEKPRS* 525
++ ENDEVL+AGFGRKGH+VGDIPGVRFKV+ V G SLLAL+ KKEKP++*
Sbjct: 293 FLSENDEVLVAGFGRKGHSVGDIPGVRFKVISVKGFSLLALYTGKKEKPKN* 138
Score = 125 bits (267), Expect = 9e-030
Identities = 59/108 (54%), Positives = 66/108 (61%)
Frame = -2 / +1
Query: 515 GFSFFSLKSASSXXXXXXXXXXXXPGMSPTA*PLRPNPAINTSSFSSM*FRQPSFGTKAA 336
GFSF + AS+ PG+SPT *P P PA TSSFS F+QPS GTKAA
Sbjct: 148 GFSFLPVYKASNEKPLTEITLNLTPGISPTE*PFLPKPATKTSSFSDKKFKQPSLGTKAA 327
Query: 335 IFFPFFTS*TRAHLRMAEFGCLASIPIFSRTMPLA*DDPAKGFFHSLP 192
IF PFF T H MAE GCLAS PIFS T+PLA*+ P KGF + P
Sbjct: 328 IFLPFFLRRTLTHFLMAELGCLASTPIFSVTIPLA*EAPMKGFLYLEP 471
>gb|CN816419.1|CN816419 HRO4520_E07_J14ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4520_E07_J14, mRNA sequence
Length = 482
Score = 85.4 bits (180), Expect(3) = 1e-035
Identities = 30/39 (76%), Positives = 35/39 (89%)
Frame = +3 / +1
Query: 417 GSCCGRHSWCQVQGC*GVWCVAACTLQGEEGEAKVLDHF 533
G+ CGR+SWCQV GC G+WCVAACTLQGEEGEA++LD F
Sbjct: 154 GARCGRYSWCQVLGCQGIWCVAACTLQGEEGEAQILDCF 270
Score = 71.2 bits (149), Expect(3) = 1e-035
Identities = 30/41 (73%), Positives = 32/41 (78%)
Frame = +1 / +1
Query: 310 QLVKNGKKIAAFVPNDGCLNYIEENDEVLIAGFGRKGHAVG 432
QL KNGK IAAFVP+DGCLN+IEENDEVLIAG G G
Sbjct: 46 QLEKNGKNIAAFVPHDGCLNFIEENDEVLIAGILASGARCG 168
Score = 69.3 bits (145), Expect(2) = 3e-026
Identities = 30/48 (62%), Positives = 36/48 (75%)
Frame = -1 / -2
Query: 555 FTPFLTTESDLRPWXXXXXLEECKQRHTRHLNNLEPDTRNVSHSMTLT 412
F P L +S+LR LEECKQRHTR+L+NLEPDTRN+SHS+ LT
Sbjct: 292 FHPSL*KKSNLRSGLLLLLLEECKQRHTRYLDNLEPDTRNISHSVPLT 149
Score = 66.1 bits (138), Expect(2) = 1e-023
Identities = 31/40 (77%), Positives = 32/40 (80%)
Frame = -3 / -1
Query: 535 RK*SKTLASPSSP*RVQAATHQTP*QP*T*HQECLPQHDP 416
+K SK ASPSSP*RVQAATHQ P*QP T*HQE LPQ P
Sbjct: 272 KKQSKIWASPSSP*RVQAATHQIP*QPRT*HQEYLPQRAP 153
Score = 63.8 bits (133), Expect(2) = 3e-026
Identities = 28/38 (73%), Positives = 33/38 (86%)
Frame = -2 / -1
Query: 404 PAINTSSFSSM*FRQPSFGTKAAIFFPFFTS*TRAHLR 291
PAI+TSSFSSM RQPS+GTKAA+FFPFF+S* + LR
Sbjct: 140 PAISTSSFSSMKLRQPSWGTKAAMFFPFFSS*HTSTLR 27
Score = 57.9 bits (120), Expect(2) = 1e-023
Identities = 23/34 (67%), Positives = 26/34 (76%)
Frame = -3 / -2
Query: 409 QIQQSTPHHSPRCSLGNHHSAQRQQSSFHSSPAE 308
+ QQS PH SP+ S GNHH AQRQQ S HSSPA+
Sbjct: 145 ECQQSAPHRSPQ*S*GNHHGAQRQQCSSHSSPAD 44
Score = 25.8 bits (50), Expect(3) = 1e-035
Identities = 10/14 (71%), Positives = 12/14 (85%)
Frame = +1 / +3
Query: 268 AKQPNSAIRKCARV 309
A+ PNSAI +CARV
Sbjct: 3 ARGPNSAIPQCARV 44
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,149,826
Number of Sequences: 8143
Number of extensions: 125645
Number of successful extensions: 1448
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 233728896
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)