TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2441110.2.2
(1019 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF084005.1|AF084005 Avena fatua ras-like small monomeric... 291 e-104
gb|CN815005.1|CN815005 HRO4504_G12_N24ZS5 Lib AA071E1X Aven... 205 3e-057
>gb|AF084005.1|AF084005 Avena fatua ras-like small monomeric GTP-binding protein (SAR1)
mRNA, complete cds
Length = 801
Score = 291 bits (630), Expect = 1e-079
Identities = 130/197 (65%), Positives = 141/197 (71%)
Frame = -1 / +1
Query: 854 DVPLGLVLRGAGLPRPVAEGGQDPLPWPRQRRQDHAAPHXXXXXXXXXXXXXXXXXXXXQ 675
DVP+GLVLRGAGL RPVAEGG DPLPWPRQRRQDHA PH Q
Sbjct: 163 DVPMGLVLRGAGLARPVAEGG*DPLPWPRQRRQDHAPPHAQGRAARAAPAHAAPNVGGAQ 342
Query: 674 HRQDQVQGVXXXXXXXXXXRVEGLLRKG*CCSIPGRCI**GAIC*IKEGARCSPV**FFG 495
HRQDQVQ + +E LLR G*CCSI GRCI GA+C*I++GARCSP **F G
Sbjct: 343 HRQDQVQSLRPRRPPDRSPSLERLLR*G*CCSILGRCIRQGALC*IEKGARCSPF**FPG 522
Query: 494 QCSISHPWQQD*YPICCL*RGATVSPRP*QLHNREGQGQPWRLQCPST*GLHVQCCSQDG 315
+ ISH WQ D*YPICCL*R A VS RP*QLHN EGQGQP LQCP++*G+HVQCC QDG
Sbjct: 523 KRPISHSWQ*D*YPICCL*RRAAVSLRP*QLHNWEGQGQPL*LQCPAS*GVHVQCCPQDG 702
Query: 314 LR*WFQVGLPVHQVGPS 264
L *W QVGLPVHQ+G S
Sbjct: 703 LW*WVQVGLPVHQLGLS 753
Score = 206 bits (444), Expect(2) = e-103
Identities = 86/114 (75%), Positives = 88/114 (77%)
Frame = +1 / -2
Query: 265 EGPT*CTGRPT*NHHRSPSCEQHCT*RPQVDGHWSRQG*PCPSRL*SC*GLGDTVAPLQR 444
E P *CTGRPT* HH SPSC QHCT* PQ GHWS +G*PCPS+L*SC*GL DT A LQR
Sbjct: 752 ESPN*CTGRPT*THHHSPSCGQHCT*TPQEAGHWSHKG*PCPSQL*SC*GLSDTAALLQR 573
Query: 445 QHMGYQSCCQG*EMEHWPKNHQTGEHRAPSLIQQIAPYHMHLPGILQHQPLRSN 606
QHMGYQS CQ *EM P NHQ GEHRAP IQQ AP MHLP ILQHQP RSN
Sbjct: 572 QHMGYQSYCQE*EMGRLPGNHQKGEHRAPFSIQQSAPCRMHLPSILQHQP*RSN 411
Score = 202 bits (436), Expect(2) = e-104
Identities = 81/81 (100%), Positives = 81/81 (100%)
Frame = -2 / +2
Query: 853 MFLWDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELS 674
MFLWDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELS
Sbjct: 164 MFLWDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELS 343
Query: 673 IGKIKFKAFDLGGHQIARRVW 611
IGKIKFKAFDLGGHQIARRVW
Sbjct: 344 IGKIKFKAFDLGGHQIARRVW 406
Score = 191 bits (411), Expect(2) = e-104
Identities = 82/97 (84%), Positives = 84/97 (86%)
Frame = -2 / +2
Query: 553 ERFAESKKEXXXXXXXXXXANVPFLILGNKIDIPYAASEEELRYHLGLSNFTTGKGKVNL 374
ERFAESKKE ANVPFLILGNKIDIPYAASEEELRYHLGLS+FTTGKGKV+L
Sbjct: 464 ERFAESKKELDALLSDDSLANVPFLILGNKIDIPYAASEEELRYHLGLSSFTTGKGKVSL 643
Query: 373 GDSNVRPLEVFMCSVVRKMGYGDGFKWVSQYIK*GPR 263
DSNVRPLEVFMCSVVRKMGYGDGFKWVSQYI *G R
Sbjct: 644 CDSNVRPLEVFMCSVVRKMGYGDGFKWVSQYIN*GSR 754
Score = 186 bits (400), Expect(2) = e-103
Identities = 74/79 (93%), Positives = 75/79 (94%)
Frame = +3 / -1
Query: 618 RRAIWWPPRSNALNLILPMLSSSDVGCCVGWCCTNRSSLSMWSSVVLPALSRPRKRILAS 797
RRAIWWPPRS ALNLILPMLSSSDVGCCVGWCCT+RSSLSMW SVVLPALSRPRKRILAS
Sbjct: 399 RRAIWWPPRSKALNLILPMLSSSDVGCCVGWCCTSRSSLSMWRSVVLPALSRPRKRILAS 220
Query: 798 FCHRPREASTP*NQSQRNI 854
FCHRP EASTP*NQS RNI
Sbjct: 219 FCHRPSEASTP*NQSHRNI 163
Score = 185 bits (398), Expect(2) = 1e-064
Identities = 80/119 (67%), Positives = 89/119 (74%)
Frame = +2 / -3
Query: 266 RALLDVLGDPLETITVAHLANNTAHEDLKWTDIGVAKVDLALPGCEVAKA*VIP*LLFRG 445
RAL+DVLGDPLE IT+AHLA+NTAHE LK DIGV K DLALP CE AKA*VIP L FRG
Sbjct: 751 RALIDVLGDPLEPITIAHLADNTAHEHLKRPDIGVTKADLALPSCEAAKA*VIPQLFFRG 572
Query: 446 SIWDINLVAKDEKWNIGQRIIRQEXXXXXXXXXXXXXXXCIYQVYYSINLCVVILPHAA 622
SIWDINL+AK+EKW++ Q IIR+E CIYQVYYSINL VVI P +A
Sbjct: 571 SIWDINLIAKNEKWDVCQGIIRKESIELLFRFSKALLVVCIYQVYYSINLSVVIFPDSA 395
Score = 163 bits (350), Expect(2) = 9e-048
Identities = 79/114 (69%), Positives = 82/114 (71%)
Frame = -3 / +3
Query: 606 ITTQRLML*YTW*MHMIRSDLLNQRRSSMLSCLMILWPMFHFSSLATRLISHMLPLKRSY 427
ITT RLML*YTW*MH RS LLN++RSSMLS LMI W HFS LA RLISHMLPLK+S
Sbjct: 411 ITTLRLML*YTW*MHTTRSALLNRKRSSMLSFLMIPWQTSHFSFLAIRLISHMLPLKKSC 590
Query: 426 GIT*ALATSQPGRARSTLATPMSVHLRSSCAVLFARWATVMVXXXXXXXXSRAL 265
GIT*ALA SQ GRARS TPMS LR SCAVL ARWA VM RAL
Sbjct: 591 GIT*ALAASQLGRARSAFVTPMSGLLRCSCAVLSARWAMVMGSSGSPSTSIRAL 752
Score = 158 bits (340), Expect = 1e-039
Identities = 67/81 (82%), Positives = 70/81 (86%)
Frame = +3 / -1
Query: 261 RRGPYLMYWETHLKPSP*PILRTTLHMKTSSGRTLESPRLTLPFPVVKLLRPR*YRXXXX 440
+R P LMYWETHL PSP*PILRTTLHM TS GRTLES RLTLPFPVVKLLRP+*YR
Sbjct: 756 QREP*LMYWETHLNPSP*PILRTTLHMNTSRGRTLESQRLTLPFPVVKLLRPK*YRSSSS 577
Query: 441 EAAYGISILLPRMRNGTLAKE 503
EAAYGISILLPRMRNGT A+E
Sbjct: 576 EAAYGISILLPRMRNGTFARE 514
Score = 77.1 bits (162), Expect(2) = 1e-064
Identities = 32/36 (88%), Positives = 34/36 (94%)
Frame = +2 / -3
Query: 716 HQPLVLEHVEQRGLAGVVEAKEEDLGLLLPQAEGGQ 823
H+PLVLEHVE+RGLAGVVEAKEEDL LLLPQAE GQ
Sbjct: 301 HEPLVLEHVEERGLAGVVEAKEEDLSLLLPQAERGQ 194
Score = 45.5 bits (93), Expect(2) = 9e-048
Identities = 19/20 (95%), Positives = 19/20 (95%)
Frame = -3 / +3
Query: 810 ACGRRRPRSSSLASTTPARP 751
ACGRRR RSSSLASTTPARP
Sbjct: 207 ACGRRRLRSSSLASTTPARP 266
>gb|CN815005.1|CN815005 HRO4504_G12_N24ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4504_G12_N24, mRNA sequence
Length = 633
Score = 205 bits (443), Expect(2) = 3e-057
Identities = 86/113 (76%), Positives = 88/113 (77%)
Frame = +1 / -1
Query: 265 EGPT*CTGRPT*NHHRSPSCEQHCT*RPQVDGHWSRQG*PCPSRL*SC*GLGDTVAPLQR 444
+ PT*CTGRPT* HH SPSC QHCT* PQ GHWS QG*PCPS+L*SC*GL DT A LQR
Sbjct: 360 KSPT*CTGRPT*THHHSPSCGQHCT*TPQEAGHWSHQG*PCPSQL*SC*GLSDTAALLQR 181
Query: 445 QHMGYQSCCQG*EMEHWPKNHQTGEHRAPSLIQQIAPYHMHLPGILQHQPLRS 603
QHMGYQS CQ *EM P NHQ GEHRAP IQQ AP MHLP ILQHQP RS
Sbjct: 180 QHMGYQSYCQE*EMGRLPGNHQKGEHRAPFSIQQSAPCRMHLPSILQHQP*RS 22
Score = 201 bits (434), Expect(2) = 6e-055
Identities = 86/112 (76%), Positives = 94/112 (83%)
Frame = -1 / +2
Query: 605 LLRKG*CCSIPGRCI**GAIC*IKEGARCSPV**FFGQCSISHPWQQD*YPICCL*RGAT 426
+LR G*CCSI GRCI GA+C*I++GARCSP **F G+ ISH WQ D*YPICCL*R A
Sbjct: 20 ILR*G*CCSILGRCIRQGALC*IEKGARCSPF**FPGKRPISHSWQ*D*YPICCL*RRAA 199
Query: 425 VSPRP*QLHNREGQGQPWRLQCPST*GLHVQCCSQDGLR*WFQVGLPVHQVG 270
VS RP*QLHN EGQGQPW LQCP++*G+HVQCC QDGL *W QVGLPVHQVG
Sbjct: 200 VSLRP*QLHNWEGQGQPW*LQCPAS*GVHVQCCPQDGLW*WVQVGLPVHQVG 355
Score = 198 bits (426), Expect(2) = 4e-053
Identities = 84/95 (88%), Positives = 85/95 (89%)
Frame = -2 / +3
Query: 553 ERFAESKKEXXXXXXXXXXANVPFLILGNKIDIPYAASEEELRYHLGLSNFTTGKGKVNL 374
ERFAESKKE ANVPFLILGNKIDIPYAASEEELRYHLGLSNFTTGKGKV+L
Sbjct: 72 ERFAESKKELDALLSDDSLANVPFLILGNKIDIPYAASEEELRYHLGLSNFTTGKGKVSL 251
Query: 373 GDSNVRPLEVFMCSVVRKMGYGDGFKWVSQYIK*G 269
GDSNVRPLEVFMCSVVRKMGYGDGFKWVSQYIK*G
Sbjct: 252 GDSNVRPLEVFMCSVVRKMGYGDGFKWVSQYIK*G 356
Score = 181 bits (389), Expect(2) = 3e-047
Identities = 79/113 (69%), Positives = 86/113 (76%)
Frame = +2 / -2
Query: 266 RALLDVLGDPLETITVAHLANNTAHEDLKWTDIGVAKVDLALPGCEVAKA*VIP*LLFRG 445
RALLDVLGDPLE IT+AHLA+NTAHE LK DIGV K DLALP CEVAKA*VIP L FRG
Sbjct: 359 RALLDVLGDPLEPITIAHLADNTAHEHLKRPDIGVTKADLALPSCEVAKA*VIPQLFFRG 180
Query: 446 SIWDINLVAKDEKWNIGQRIIRQEXXXXXXXXXXXXXXXCIYQVYYSINLCVV 604
SIWDINL+AK+EKW++ Q IIR+E CIYQVYYSINL VV
Sbjct: 179 SIWDINLIAKNEKWDVCQGIIRKESIELLFRFSKALLVVCIYQVYYSINLSVV 21
Score = 166 bits (356), Expect(2) = 2e-043
Identities = 81/113 (71%), Positives = 84/113 (74%)
Frame = -3 / +1
Query: 603 TTQRLML*YTW*MHMIRSDLLNQRRSSMLSCLMILWPMFHFSSLATRLISHMLPLKRSYG 424
TT RLML*YTW*MH RS LLN++RSSMLS LMI W HFS LA RLISHMLPLK+S G
Sbjct: 22 TTLRLML*YTW*MHTTRSALLNRKRSSMLSFLMIPWQTSHFSFLAIRLISHMLPLKKSCG 201
Query: 423 IT*ALATSQPGRARSTLATPMSVHLRSSCAVLFARWATVMVXXXXXXXXSRAL 265
IT*ALATSQ GRARS L TPMS LR SCAVL ARWA VM SRAL
Sbjct: 202 IT*ALATSQLGRARSALVTPMSGLLRCSCAVLSARWAMVMGSSGSPSTSSRAL 360
Score = 165 bits (355), Expect = 1e-041
Identities = 68/78 (87%), Positives = 70/78 (89%)
Frame = +3 / -3
Query: 270 PYLMYWETHLKPSP*PILRTTLHMKTSSGRTLESPRLTLPFPVVKLLRPR*YRXXXXEAA 449
PYLMYWETHL PSP*PILRTTLHM TS GRTLESPRLTLPFPVVKLLRP+*YR EAA
Sbjct: 355 PYLMYWETHLNPSP*PILRTTLHMNTSRGRTLESPRLTLPFPVVKLLRPK*YRSSSSEAA 176
Query: 450 YGISILLPRMRNGTLAKE 503
YGISILLPRMRNGT A+E
Sbjct: 175 YGISILLPRMRNGTFARE 122
Score = 31.8 bits (63), Expect(2) = 3e-057
Identities = 13/16 (81%), Positives = 13/16 (81%)
Frame = +2 / -3
Query: 122 QHVQQC*ALFFLFIPS 169
QH QC*ALFF FIPS
Sbjct: 505 QHGTQC*ALFFSFIPS 458
Score = 28.1 bits (55), Expect(2) = 6e-055
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = -3 / +3
Query: 177 SIMEGMKRKNSA*HC*TC*V 118
++ EGMK KN+A*H C*+
Sbjct: 450 TVSEGMKEKNNA*HWVPC*I 509
Score = 25.8 bits (50), Expect(2) = 4e-053
Identities = 12/18 (66%), Positives = 13/18 (72%)
Frame = -1 / +1
Query: 167 RV*RGKTVLNIVEHVESL 114
RV*+ KT LNI H ESL
Sbjct: 460 RV*KRKTTLNIGYHAESL 513
Score = 25.4 bits (49), Expect(2) = 2e-043
Identities = 11/16 (68%), Positives = 12/16 (75%)
Frame = -2 / +2
Query: 166 GYEEEKQCLTLLNMLS 119
GYE EKQ LTL ML+
Sbjct: 461 GYEREKQRLTLGTMLN 508
Score = 23.1 bits (44), Expect(2) = 3e-047
Identities = 10/18 (55%), Positives = 11/18 (61%)
Frame = +1 / -2
Query: 115 KDSTCSTMLSTVFPLHTL 168
+DS MLS VF HTL
Sbjct: 512 RDSAWYPMLSVVFLFHTL 459
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,396,258
Number of Sequences: 8143
Number of extensions: 123967
Number of successful extensions: 845
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 356712525
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)