TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2440958.2.1
(791 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815034.1|CN815034 HRO4505_B06_C11ZS5 Lib AA071E1X Aven... 116 9e-039
gb|CN816101.1|CN816101 HRO4517_A05_A09ZS5 Lib AA071E1X Aven... 106 8e-038
gb|CN816873.1|CN816873 HRO4525_G05_M09ZS5 Lib AA071E1X Aven... 78 2e-030
gb|CN814875.1|CN814875 HRO4503_D10_H19ZS5 Lib AA071E1X Aven... 118 9e-028
gb|CN821053.1|CN821053 HRO4427_A04_B07ZS5 Lib AA069E1X Aven... 77 5e-027
gb|CN815850.1|CN815850 HRO4511_B09_D17ZS5 Lib AA071E1X Aven... 77 9e-027
gb|CN814955.1|CN814955 HRO4504_C09_F18ZS5 Lib AA071E1X Aven... 74 4e-023
>gb|CN815034.1|CN815034 HRO4505_B06_C11ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4505_B06_C11, mRNA sequence
Length = 629
Score = 116 bits (248), Expect(3) = 9e-039
Identities = 45/85 (52%), Positives = 58/85 (68%)
Frame = +3 / +1
Query: 63 DVGGGFGGATQAIAKAFPHLECSVLDLPNVVAGAPADTAVKYVAGDMFESVSSADAVFLK 242
D GGG +AI KA PH++C+VLDLP V+ P+D V YVAGD+F SV + AV LK
Sbjct: 7 DCGGGD*TTARAITKAHPHIKCTVLDLPKVIEKVPSDGIVNYVAGDLFHSVPKSQAVLLK 186
Query: 243 SIIHDWGDADCVKILKNCKKAIPAQ 317
++H W D DCVKIL C+ AIP++
Sbjct: 187 LVLHHWSDDDCVKILSQCRSAIPSR 261
Score = 49.2 bits (101), Expect(3) = 9e-039
Identities = 15/40 (37%), Positives = 27/40 (67%)
Frame = +3 / +1
Query: 414 IMTINGVERDEREWKKIIFEAGFTSYKIIPVLGTRSIIEV 533
++ G +RDE +W+++ +AGF+ YK++ LG R + EV
Sbjct: 358 LVNTRGRQRDENDWRQLFMKAGFSDYKVVKKLGARGVFEV 477
Score = 27.6 bits (54), Expect(3) = 9e-039
Identities = 10/13 (76%), Positives = 13/13 (100%)
Frame = +3 / +1
Query: 315 QGGKVIILDIVVG 353
+GGKVII+DI+VG
Sbjct: 265 EGGKVIIIDILVG 303
>gb|CN816101.1|CN816101 HRO4517_A05_A09ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4517_A05_A09, mRNA sequence
Length = 652
Score = 106 bits (226), Expect(3) = 8e-038
Identities = 39/71 (54%), Positives = 52/71 (73%)
Frame = +3 / +3
Query: 105 KAFPHLECSVLDLPNVVAGAPADTAVKYVAGDMFESVSSADAVFLKSIIHDWGDADCVKI 284
KA+PHL+C+VLDLP V+ AP + YVAGD+F +V + AV LK ++H W D DCVKI
Sbjct: 15 KAYPHLKCTVLDLPKVIEKAPPHDVINYVAGDLFHTVPPSQAVMLKLVLHFWSDEDCVKI 194
Query: 285 LKNCKKAIPAQ 317
L C+KAIP++
Sbjct: 195 LAQCRKAIPSR 227
Score = 59.3 bits (123), Expect(3) = 8e-038
Identities = 22/45 (48%), Positives = 32/45 (71%)
Frame = +3 / +3
Query: 408 LYIMTINGVERDEREWKKIIFEAGFTSYKIIPVLGTRSIIEVCP* 542
L ++ G +RDE+ W+++ +AGFT YKI+ LG RS+IEV P*
Sbjct: 318 LMMVNTRGGQRDEKHWRELFMQAGFTDYKIVKKLGARSVIEVYP* 452
Score = 24.4 bits (47), Expect(3) = 8e-038
Identities = 9/12 (75%), Positives = 12/12 (100%)
Frame = +3 / +3
Query: 315 QGGKVIILDIVV 350
+GGKVII++IVV
Sbjct: 231 EGGKVIIIEIVV 266
>gb|CN816873.1|CN816873 HRO4525_G05_M09ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4525_G05_M09, mRNA sequence
Length = 662
Score = 78.0 bits (164), Expect(4) = 2e-030
Identities = 29/56 (51%), Positives = 38/56 (67%)
Frame = +3 / +2
Query: 150 VVAGAPADTAVKYVAGDMFESVSSADAVFLKSIIHDWGDADCVKILKNCKKAIPAQ 317
V+ P+D V YVAGD+F SV + AV LK ++H W D DCVKIL C+ AIP++
Sbjct: 41 VIEKVPSDGIVNYVAGDLFHSVPKSQAVLLKLVLHHWSDDDCVKILSQCRSAIPSR 208
Score = 49.2 bits (101), Expect(4) = 2e-030
Identities = 15/40 (37%), Positives = 27/40 (67%)
Frame = +3 / +2
Query: 414 IMTINGVERDEREWKKIIFEAGFTSYKIIPVLGTRSIIEV 533
++ G +RDE +W+++ +AGF+ YK++ LG R + EV
Sbjct: 305 LVNTRGRQRDENDWRQLFMKAGFSDYKVVKKLGARGVFEV 424
Score = 27.6 bits (54), Expect(4) = 2e-030
Identities = 10/13 (76%), Positives = 13/13 (100%)
Frame = +3 / +2
Query: 315 QGGKVIILDIVVG 353
+GGKVII+DI+VG
Sbjct: 212 EGGKVIIIDILVG 250
Score = 27.2 bits (53), Expect(4) = 2e-030
Identities = 8/11 (72%), Positives = 11/11 (100%)
Frame = +3 / +3
Query: 114 PHLECSVLDLP 146
PH++C+VLDLP
Sbjct: 6 PHIKCTVLDLP 38
>gb|CN814875.1|CN814875 HRO4503_D10_H19ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4503_D10_H19, mRNA sequence
Length = 846
Score = 118 bits (253), Expect = 9e-028
Identities = 51/94 (54%), Positives = 65/94 (69%)
Frame = +3 / +1
Query: 261 GDADCVKILKNCKKAIPAQGGKVIILDIVVGAGSSSCDRKNVETQCLFDLYIMTINGVER 440
GD DCVKILK CK+AIP++ ++ + + GS D ETQ FDL+IM + G+ER
Sbjct: 10 GDEDCVKILKKCKEAIPSRDAGGKVIIVDMVVGSGPNDIVTRETQVFFDLFIMYLEGIER 189
Query: 441 DEREWKKIIFEAGFTSYKIIPVLGTRSIIEVCP* 542
+E EWKKI EAGF+ YKII VLG RS+IE+ P*
Sbjct: 190 EEFEWKKIFMEAGFSEYKIISVLGVRSVIELYP* 291
>gb|CN821053.1|CN821053 HRO4427_A04_B07ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4427_A04_B07, mRNA
sequence
Length = 644
Score = 76.7 bits (161), Expect(3) = 5e-027
Identities = 28/51 (54%), Positives = 36/51 (70%)
Frame = +3 / +3
Query: 165 PADTAVKYVAGDMFESVSSADAVFLKSIIHDWGDADCVKILKNCKKAIPAQ 317
P+D V YVAGD+F SV + AV LK ++H W D DCVKIL C+ AIP++
Sbjct: 24 PSDGIVNYVAGDLFHSVPKSQAVLLKLVLHHWSDEDCVKILSQCRNAIPSR 176
Score = 49.6 bits (102), Expect(3) = 5e-027
Identities = 16/40 (40%), Positives = 27/40 (67%)
Frame = +3 / +3
Query: 414 IMTINGVERDEREWKKIIFEAGFTSYKIIPVLGTRSIIEV 533
++ G +RDE +W+++ +AGF+ YKI+ LG R + EV
Sbjct: 273 LVNTRGRQRDENDWRQLFMKAGFSDYKIVKKLGARGVFEV 392
Score = 27.2 bits (53), Expect(3) = 5e-027
Identities = 10/13 (76%), Positives = 13/13 (100%)
Frame = +3 / +3
Query: 315 QGGKVIILDIVVG 353
+GGKVII+DI+VG
Sbjct: 180 KGGKVIIIDILVG 218
>gb|CN815850.1|CN815850 HRO4511_B09_D17ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4511_B09_D17, mRNA sequence
Length = 621
Score = 76.7 bits (161), Expect(3) = 9e-027
Identities = 29/51 (56%), Positives = 36/51 (70%)
Frame = +3 / +2
Query: 165 PADTAVKYVAGDMFESVSSADAVFLKSIIHDWGDADCVKILKNCKKAIPAQ 317
P+D V YVAGD+F SV + AV LK ++H W D DCVKIL CK AIP++
Sbjct: 2 PSDGIVYYVAGDLFHSVPKSQAVMLKLVLHRWSDEDCVKILSQCKSAIPSR 154
Score = 48.3 bits (99), Expect(3) = 9e-027
Identities = 15/40 (37%), Positives = 27/40 (67%)
Frame = +3 / +2
Query: 414 IMTINGVERDEREWKKIIFEAGFTSYKIIPVLGTRSIIEV 533
++ G +RDE +W+++ +AGF+ YKI+ +G R + EV
Sbjct: 251 LVNTRGRQRDENDWRQLFMKAGFSDYKIVKKVGARGVFEV 370
Score = 27.6 bits (54), Expect(3) = 9e-027
Identities = 10/13 (76%), Positives = 13/13 (100%)
Frame = +3 / +2
Query: 315 QGGKVIILDIVVG 353
+GGKVII+DI+VG
Sbjct: 158 EGGKVIIIDILVG 196
>gb|CN814955.1|CN814955 HRO4504_C09_F18ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4504_C09_F18, mRNA sequence
Length = 648
Score = 73.9 bits (155), Expect(2) = 4e-023
Identities = 31/63 (49%), Positives = 41/63 (65%)
Frame = +3 / +1
Query: 180 VKYVAGDMFESVSSADAVFLKSIIHDWGDADCVKILKNCKKAIPAQGGKVIILDIVVGAG 359
V YVAGDMF+ V A AV +K ++H W D DCVKIL CKKA+P++ ++ I +
Sbjct: 16 VNYVAGDMFKLVPPAQAVLVKLVLHHWSDEDCVKILAQCKKAVPSREEGGKVIVIDIVVD 195
Query: 360 SSS 368
SSS
Sbjct: 196 SSS 204
Score = 48.7 bits (100), Expect(2) = 4e-023
Identities = 19/39 (48%), Positives = 25/39 (64%)
Frame = +3 / +1
Query: 426 NGVERDEREWKKIIFEAGFTSYKIIPVLGTRSIIEVCP* 542
NG +RDE +W I +AGF+ Y I+ LG R + EV P*
Sbjct: 259 NGRQRDESDWGDIFTKAGFSGYNIVKKLGARGVFEVYP* 375
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,070,930
Number of Sequences: 8143
Number of extensions: 105732
Number of successful extensions: 933
Number of sequences better than 1.0e-020: 8
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 267814360
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)