TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2440015.2.2
(605 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN818423.1|CN818423 HRO4453_C09_F17ZS5 Lib AA070E1X Aven... 175 5e-045
gb|CN820760.1|CN820760 HRO4414_D11_G22ZS5 Lib AA069E1X Aven... 175 5e-045
>gb|CN818423.1|CN818423 HRO4453_C09_F17ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4453_C09_F17, mRNA sequence
Length = 619
Score = 175 bits (377), Expect = 5e-045
Identities = 72/81 (88%), Positives = 75/81 (92%)
Frame = -3 / +3
Query: 504 AAVKMIIPVRCFTCGKVIGNKWDLYLDLLQADYSEGDALDALELFRYCCRRMLMTHVDLI 325
AA KMIIPVRCFTCGKVIGNKWD YLDLLQADY+EGDALDAL L RYCCRRMLMTHVDLI
Sbjct: 57 AASKMIIPVRCFTCGKVIGNKWDHYLDLLQADYTEGDALDALGLVRYCCRRMLMTHVDLI 236
Query: 324 EKLLNYNTLEKTETS*ASTSC 262
EKLLNYNTLEKTET+* + SC
Sbjct: 237 EKLLNYNTLEKTETT*LNPSC 299
Score = 101 bits (215), Expect = 1e-022
Identities = 48/60 (80%), Positives = 50/60 (83%)
Frame = +2 / -1
Query: 326 MRSTCVMSIRLQQ*RNNSKASRASPSE*SAWRRSRYRSHLFPITLPQVKQRTGIIIFTAA 505
+RST VMSIR QQ*R KASRASPS *SAWRRSR SHL PITLPQVKQRTG+IIF AA
Sbjct: 235 IRSTWVMSIRRQQ*RTKPKASRASPSV*SAWRRSR*WSHLLPITLPQVKQRTGMIIFDAA 56
Score = 85.8 bits (181), Expect(2) = 6e-021
Identities = 40/69 (57%), Positives = 47/69 (68%)
Frame = +3 / -2
Query: 255 FWSMMCSLNLSRSSLGCCS*ATSQ*GQHVS*AFACSSSGTIPKHPEHPLLSSRPGGGRGI 434
F S M S+ LS S CCS*ATS+*GQH S*A+ SSS P+HPEHPL +RPG +G
Sbjct: 306 FHSRMGSIKLSPFSPVCCS*ATSR*GQHGS*AYDGSSSEPSPRHPEHPLPCNRPGEDQGS 127
Query: 435 DPTCSQ*PC 461
PTC + PC
Sbjct: 126 GPTCYRSPC 100
Score = 29.0 bits (57), Expect(2) = 6e-021
Identities = 11/14 (78%), Positives = 12/14 (85%)
Frame = +1 / -3
Query: 460 AAGEAAHWDNHLHR 501
AAGEAAH D+HL R
Sbjct: 101 AAGEAAHGDDHLRR 60
>gb|CN820760.1|CN820760 HRO4414_D11_G22ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4414_D11_G22, mRNA
sequence
Length = 498
Score = 175 bits (377), Expect = 5e-045
Identities = 72/81 (88%), Positives = 75/81 (92%)
Frame = -3 / +2
Query: 504 AAVKMIIPVRCFTCGKVIGNKWDLYLDLLQADYSEGDALDALELFRYCCRRMLMTHVDLI 325
AA KMIIPVRCFTCGKVIGNKWD YLDLLQADY+EGDALDAL L RYCCRRMLMTHVDLI
Sbjct: 35 AASKMIIPVRCFTCGKVIGNKWDHYLDLLQADYTEGDALDALGLVRYCCRRMLMTHVDLI 214
Query: 324 EKLLNYNTLEKTETS*ASTSC 262
EKLLNYNTLEKTET+* + SC
Sbjct: 215 EKLLNYNTLEKTETT*LNPSC 277
Score = 101 bits (215), Expect = 1e-022
Identities = 48/60 (80%), Positives = 50/60 (83%)
Frame = +2 / -1
Query: 326 MRSTCVMSIRLQQ*RNNSKASRASPSE*SAWRRSRYRSHLFPITLPQVKQRTGIIIFTAA 505
+RST VMSIR QQ*R KASRASPS *SAWRRSR SHL PITLPQVKQRTG+IIF AA
Sbjct: 213 IRSTWVMSIRRQQ*RTKPKASRASPSV*SAWRRSR*WSHLLPITLPQVKQRTGMIIFDAA 34
Score = 85.8 bits (181), Expect(2) = 6e-021
Identities = 40/69 (57%), Positives = 47/69 (68%)
Frame = +3 / -2
Query: 255 FWSMMCSLNLSRSSLGCCS*ATSQ*GQHVS*AFACSSSGTIPKHPEHPLLSSRPGGGRGI 434
F S M S+ LS S CCS*ATS+*GQH S*A+ SSS P+HPEHPL +RPG +G
Sbjct: 284 FHSRMGSIKLSPFSPVCCS*ATSR*GQHGS*AYDGSSSEPSPRHPEHPLPCNRPGEDQGS 105
Query: 435 DPTCSQ*PC 461
PTC + PC
Sbjct: 104 GPTCYRSPC 78
Score = 29.0 bits (57), Expect(2) = 6e-021
Identities = 11/14 (78%), Positives = 12/14 (85%)
Frame = +1 / -3
Query: 460 AAGEAAHWDNHLHR 501
AAGEAAH D+HL R
Sbjct: 79 AAGEAAHGDDHLRR 38
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,148,136
Number of Sequences: 8143
Number of extensions: 101243
Number of successful extensions: 1633
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 194851479
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)