TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2437779.2.1
(3727 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN819884.1|CN819884 HRO4407_C09_F17ZS5 Lib AA069E1X Aven... 208 4e-054
gb|CN819594.1|CN819594 HRO4401_H12_O23ZS5 Lib AA069E1X Aven... 184 7e-047
gb|CN815813.1|CN815813 HRO4510_G06_M12ZS5 Lib AA071E1X Aven... 181 6e-046
gb|CN815647.1|CN815647 HRO4514_A01_A02ZS5 Lib AA071E1X Aven... 115 6e-026
>gb|CN819884.1|CN819884 HRO4407_C09_F17ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4407_C09_F17, mRNA
sequence
Length = 676
Score = 208 bits (449), Expect = 4e-054
Identities = 82/99 (82%), Positives = 91/99 (91%)
Frame = -2 / +1
Query: 516 VGVVAGISYAINSGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGKQNRTPTIVVVWAILL 337
VGVVAG SYAINSGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMG+ NRTPTIV+VWAILL
Sbjct: 55 VGVVAGTSYAINSGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRTNRTPTIVIVWAILL 234
Query: 336 ASIFSLMWVRIDPFTTRVTGPDIAKCGINC*DELKIIKE 220
ASIFSL+WVRIDPFTTRV GP+I CGINC*+ ++ ++
Sbjct: 235 ASIFSLLWVRIDPFTTRVAGPNIQTCGINC*ENGRLQRQ 351
Score = 186 bits (401), Expect = 2e-047
Identities = 79/104 (75%), Positives = 89/104 (85%)
Frame = +2 / -3
Query: 248 QLMPHFAISGPVTRVVNGSIRTHIREKIDARRIAQTTTMVGVRFCFPMRPLRKGYRWTIT 427
QL+P + GP TRVVNGSIRTH +EKI+ARRIAQT T+VGVRF P+RPLR GY+ TIT
Sbjct: 323 QLIPQVWMFGPATRVVNGSIRTHSKEKIEARRIAQTMTIVGVRFVLPIRPLRNGYK*TIT 144
Query: 428 QNAKKSFPKRGPHDW*PLLIA*EMPATTPTRLMIKIVVGGIRSV 559
Q AKKSFP GPHDW*PLL+A*E+PATTPT LMIKIVVGG+RSV
Sbjct: 143 QKAKKSFPNSGPHDW*PLLMA*EVPATTPTMLMIKIVVGGMRSV 12
Score = 152 bits (326), Expect = 4e-037
Identities = 63/106 (59%), Positives = 79/106 (74%)
Frame = +1 / -2
Query: 238 LILAVDATFRDIRTSDPGGEWIDTNPHQGKDRRKEDSPDNDNGRRAVLLPHEALEEGVQV 417
+ LAVD T D+R S+PG EWI+TNP QG+DR +EDSPD+DN R AV H+ LEE V V
Sbjct: 333 IFLAVDPTGLDVRASNPGSEWINTNPQQGEDRSEEDSPDDDNRRCAVCPAHKTLEEWV*V 154
Query: 418 DNHPERKEELPEKRSP*LVTAIDCVGNASDHADQVDDQNSGWWDQK 555
+NHPE KEEL E+R P*LVT +D +G AS++ D V+DQN W D+K
Sbjct: 153 NNHPEGKEELSEQRPP*LVTTVDGIGGASNNTDHVNDQNCCWRDEK 16
Score = 142 bits (304), Expect = 4e-034
Identities = 56/106 (52%), Positives = 72/106 (67%)
Frame = -1 / +3
Query: 559 NASDPTNHYFDHQPGRRGRWHFLRNQ*RLPVMGTSFREALLCVLGDCPPVPLPQGPHGEA 380
+ S P+N+ FDH GR WH L +Q LP+MG + R+ALLC+LGDC +P+PQG +G+
Sbjct: 12 DTSHPSNNNFDH*HGRCCCWHLLCHQQWLPIMGAAVRKALLCLLGDCSLIPIPQGSYGQD 191
Query: 379 EPHADHCRCLGYPPCVDLFPDVGSYRSIHHPGHWS*YREMWHQLLG 242
+PH D C LGYPP DL VGSY SIH+PG W + +W QLLG
Sbjct: 192 KPHTDDCHRLGYPPRFDLLLAVGSY*SIHYPGCWPEHPNLWDQLLG 329
>gb|CN819594.1|CN819594 HRO4401_H12_O23ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4401_H12_O23, mRNA
sequence
Length = 620
Score = 184 bits (397), Expect = 7e-047
Identities = 71/87 (81%), Positives = 80/87 (91%)
Frame = -2 / +2
Query: 480 SGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGKQNRTPTIVVVWAILLASIFSLMWVRID 301
SGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMG+ NRTPTIV+VWAILLASIFSL+WVRID
Sbjct: 2 SGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRTNRTPTIVIVWAILLASIFSLLWVRID 181
Query: 300 PFTTRVTGPDIAKCGINC*DELKIIKE 220
PFTTRV GP+I CGINC*+ ++ ++
Sbjct: 182 PFTTRVAGPNIQTCGINC*ENGRLQRQ 262
Score = 141 bits (302), Expect = 8e-034
Identities = 58/78 (74%), Positives = 65/78 (83%)
Frame = +2 / -3
Query: 248 QLMPHFAISGPVTRVVNGSIRTHIREKIDARRIAQTTTMVGVRFCFPMRPLRKGYRWTIT 427
QL+P + GP TRVVNGSIRTH +EKI+ARRIAQT T+VGVRF P+RPLR GY+ TIT
Sbjct: 234 QLIPQVWMFGPATRVVNGSIRTHSKEKIEARRIAQTMTIVGVRFVLPIRPLRNGYK*TIT 55
Query: 428 QNAKKSFPKRGPHDW*PL 481
Q AKKSFPK GPHDW*PL
Sbjct: 54 QKAKKSFPKSGPHDW*PL 1
Score = 122 bits (261), Expect = 4e-028
Identities = 52/80 (65%), Positives = 61/80 (76%)
Frame = +1 / -2
Query: 238 LILAVDATFRDIRTSDPGGEWIDTNPHQGKDRRKEDSPDNDNGRRAVLLPHEALEEGVQV 417
+ LAVD T D+R SDPG EWIDTNP QG+DR +EDSPD+D+ R AV H+ LEE V V
Sbjct: 244 IFLAVDPTGLDVRASDPGSEWIDTNPQQGEDRSEEDSPDDDDRRCAVCPAHKTLEERV*V 65
Query: 418 DNHPERKEELPEKRSP*LVT 477
+NHPE KEEL E+R P*LVT
Sbjct: 64 NNHPEGKEELSEERPP*LVT 5
Score = 118 bits (252), Expect = 7e-027
Identities = 44/78 (56%), Positives = 56/78 (71%)
Frame = -1 / +1
Query: 475 LPVMGTSFREALLCVLGDCPPVPLPQGPHGEAEPHADHCRCLGYPPCVDLFPDVGSYRSI 296
LP+MG + R+ALLC+LGDC +P+PQG +G+ +PH D LGYPP DL VGSYRSI
Sbjct: 7 LPIMGAALRKALLCLLGDCSLIPVPQGSYGQDKPHTDDRHRLGYPPRFDLLLAVGSYRSI 186
Query: 295 HHPGHWS*YREMWHQLLG 242
H+PG W + +W QLLG
Sbjct: 187 HYPGRWPEHPNLWDQLLG 240
>gb|CN815813.1|CN815813 HRO4510_G06_M12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4510_G06_M12, mRNA sequence
Length = 521
Score = 181 bits (390), Expect = 6e-046
Identities = 68/91 (74%), Positives = 81/91 (89%)
Frame = -2 / +2
Query: 516 VGVVAGISYAINSGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGKQNRTPTIVVVWAILL 337
VG+VAG+S A+N+GY SWGPLFGKLFF+FWVIVHLYPFLKGLMG+QNRTPTIVV+W++LL
Sbjct: 62 VGIVAGVSDAVNNGYGSWGPLFGKLFFSFWVIVHLYPFLKGLMGRQNRTPTIVVLWSVLL 241
Query: 336 ASIFSLMWVRIDPFTTRVTGPDIAKCGINC* 244
ASIFSL+WVRIDPF + GP + CG+ C*
Sbjct: 242 ASIFSLVWVRIDPFIAKSKGPILKPCGVQC* 334
Score = 150 bits (323), Expect = 1e-036
Identities = 66/103 (64%), Positives = 73/103 (70%)
Frame = +2 / -3
Query: 257 PHFAISGPVTRVVNGSIRTHIREKIDARRIAQTTTMVGVRFCFPMRPLRKGYRWTITQNA 436
PH GP+ + GSI T REK++A R Q+TTMVGVRFC PM PLR G RWT+TQ
Sbjct: 321 PHGLRMGPLDLAMKGSILTQTREKMEASRTDQSTTMVGVRFCLPMSPLRNG*RWTMTQKE 142
Query: 437 KKSFPKRGPHDW*PLLIA*EMPATTPTRLMIKIVVGGIRSVVH 565
KKSFPKRGPHD *PLL A E PAT PT LM+ VVGGI VVH
Sbjct: 141 KKSFPKRGPHDP*PLLTASETPATMPTMLMMMSVVGGISRVVH 13
Score = 126 bits (270), Expect = 2e-029
Identities = 51/94 (54%), Positives = 68/94 (72%)
Frame = +1 / -2
Query: 289 GGEWIDTNPHQGKDRRKEDSPDNDNGRRAVLLPHEALEEGVQVDNHPERKEELPEKRSP* 468
G E I+ +P +G+D ++D P++D+G RAVL PHE LEE V+VD+ PE KEELPE+ +P*
Sbjct: 289 GDEGINPDPDEGEDGGEQDRPEHDDGGRAVLPPHEPLEEWVEVDDDPEGKEELPEEGAP* 110
Query: 469 LVTAIDCVGNASDHADQVDDQNSGWWDQKRCPLE 570
V +DCVGN S++AD VDD WWDQ PL+
Sbjct: 109 PVAIVDCVGNTSNNADHVDDDECSWWDQ*GGPLK 8
Score = 105 bits (225), Expect = 3e-023
Identities = 47/91 (51%), Positives = 58/91 (63%)
Frame = -1 / +1
Query: 565 VDNASDPTNHYFDHQPGRRGRWHFLRNQ*RLPVMGTSFREALLCVLGDCPPVPLPQGPHG 386
VD+ +DPTN+ HQ GR W F R+Q L VMG REALL +LG PP+P+PQG HG
Sbjct: 13 VDHPTDPTNYTHHHQHGRHCCWCFRRSQQWLRVMGPPLREALLFLLGHRPPLPIPQGAHG 192
Query: 385 EAEPHADHCRCLGYPPCVDLFPDVGSYRSIH 293
EA+PHA H R L P + L P +G S+H
Sbjct: 193 EAKPHAHHRRALVCPARLHLLPRLGQD*SLH 285
>gb|CN815647.1|CN815647 HRO4514_A01_A02ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4514_A01_A02, mRNA sequence
Length = 474
Score = 115 bits (245), Expect = 6e-026
Identities = 41/60 (68%), Positives = 53/60 (88%)
Frame = -2 / +3
Query: 426 VIVHLYPFLKGLMGKQNRTPTIVVVWAILLASIFSLMWVRIDPFTTRVTGPDIAKCGINC 247
VIVHLYPFLKGL+G+QNRTPTIV+VW+ILLASI SL+WVR++PF + GP + +CG++C
Sbjct: 12 VIVHLYPFLKGLLGRQNRTPTIVIVWSILLASIISLLWVRVNPFLAKTDGPTLEECGLDC 191
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 43,987,572
Number of Sequences: 8143
Number of extensions: 714702
Number of successful extensions: 4156
Number of sequences better than 1.0e-020: 4
length of database: 1,567,487
effective HSP length: 47
effective length of database: 1,184,766
effective search space used: 1414610604
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)