TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2437739.2.2
(2231 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816985.1|CN816985 HRO4527_A03_B05ZS5 Lib AA071E1X Aven... 153 1e-063
gb|CN820177.1|CN820177 HRO4412_A07_B14ZS5 Lib AA069E1X Aven... 114 3e-062
gb|BE439135.1|BE439135 CDO465_WHE2G0092F ITEC CDO Oat cDNA ... 98 5e-021
>gb|CN816985.1|CN816985 HRO4527_A03_B05ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4527_A03_B05, mRNA sequence
Length = 608
Score = 153 bits (328), Expect(2) = 1e-063
Identities = 64/137 (46%), Positives = 85/137 (62%)
Frame = +3 / +1
Query: 1365 KNGKPGDAPLLPLPKKAARILVAGSHADNLGYQCGGWTIEWQGDXXXXXXXXXXLDAVKA 1544
++GK LPL K A RILVAG+HAD++GYQCGGWTI W GD L+A++
Sbjct: 7 EDGKNLKETFLPLAKNAKRILVAGTHADDIGYQCGGWTIAWHGDSGKITLGTSILEAIQE 186
Query: 1545 AVDPSTEVVFAESPDAEFVRSGGFSYAIVAVGEHPYTETKGDSMNLTIPDPGPSTVQTVC 1724
+V TEVV + P + +GGFSYA+V VGE PY E GD +L+IP G + V
Sbjct: 187 SVGVETEVVHEQCPTDAIIETGGFSYAVVVVGEVPYAEWLGDRTDLSIPFNGSDLITRVA 366
Query: 1725 AAVRCVTVLISGRPVVI 1775
+ V + ++ISGRP+VI
Sbjct: 367 SKVPTLVIVISGRPLVI 417
Score = 106 bits (227), Expect(2) = 1e-063
Identities = 41/65 (63%), Positives = 50/65 (76%)
Frame = +3 / +1
Query: 1767 VVIQPFLGAMDAVVAAWLPGTEGQGVTDVLFGDYGFTGKLPRTWFRSVDQLPMNYGDAHY 1946
V+ Q + +DA+VAAWLPG+EG G+TD +FGD+ F G LP TWFRS DQLP+N GDA Y
Sbjct: 412 VIDQQVMEKIDALVAAWLPGSEGTGITDCIFGDHDFVGTLPVTWFRSSDQLPINIGDAKY 591
Query: 1947 DPLFP 1961
D LFP
Sbjct: 592 DLLFP 606
Score = 84.9 bits (179), Expect(2) = 3e-030
Identities = 53/135 (39%), Positives = 66/135 (48%)
Frame = -2 / -1
Query: 1774 ITTGRPLMSTVTHRTAAHTVCTVLGPGSGIVRFMLSPLVSVYGCSPTATIAYEKPPLLTN 1595
IT G P M+ + T T P GI+R +LSP S G SPT T A E PP+
Sbjct: 416 ITKGLPDMTITSVGTLLATRVIRSEPLKGILRSVLSPNHSA*GTSPTTTTA*ENPPVSMI 237
Query: 1594 SASGLSANTTSVDGSTAAFTAXXXXXXXXXXXXXPCHSIVHPPHWYPRLSAWLPATRILA 1415
++ G + TTSV T + A PC +IVHPPH YP SA +PAT I
Sbjct: 236 ASVGHCSCTTSVSTPTDSCMASSMLVPRVIFPLSPCQAIVHPPH*YPISSACVPATSIRF 57
Query: 1414 AFLGRGSSGASPGLP 1370
AFL G + + LP
Sbjct: 56 AFLANGRNVSFRFLP 12
Score = 63.4 bits (132), Expect(2) = 3e-030
Identities = 29/49 (59%), Positives = 33/49 (67%)
Frame = -2 / -1
Query: 1939 ASP*FIGSWSTDLNHVRGSFPVNP*SPNSTSVTPWPSVPGSQAATTASM 1793
ASP FIG+ S DLNHV G+ P SP SV P PS+PGS AAT AS+
Sbjct: 584 ASPIFIGN*SEDLNHVTGNVPTKSWSPKMQSVIPVPSLPGSHAATRASI 438
>gb|CN820177.1|CN820177 HRO4412_A07_B14ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4412_A07_B14, mRNA
sequence
Length = 675
Score = 114 bits (243), Expect(3) = 3e-062
Identities = 43/70 (61%), Positives = 52/70 (74%)
Frame = +3 / +1
Query: 1767 VVIQPFLGAMDAVVAAWLPGTEGQGVTDVLFGDYGFTGKLPRTWFRSVDQLPMNYGDAHY 1946
V+ L +DA+VAAWLPG+EG G+TD +FGD+ F G LP TWFR DQ P+N GDA Y
Sbjct: 400 VIDPQVLEKIDALVAAWLPGSEGNGITDCIFGDHDFVGTLPVTWFRFSDQFPINIGDAKY 579
Query: 1947 DPLFPLGFGL 1976
DPLFP G+GL
Sbjct: 580 DPLFPFGYGL 609
Score = 87.7 bits (185), Expect(3) = 3e-062
Identities = 37/85 (43%), Positives = 53/85 (62%)
Frame = +3 / +1
Query: 1527 LDAVKAAVDPSTEVVFAESPDAEFVRSGGFSYAIVAVGEHPYTETKGDSMNLTIPDPGPS 1706
L+A++ +V TEVV + P + +GGFSYA+V VGE PY E GD +L+IP G
Sbjct: 157 LEAIQESVGVETEVVHEQCPTDAIIETGGFSYAVVVVGEVPYAEWLGDRTDLSIPFNGSD 336
Query: 1707 TVQTVCAAVRCVTVLISGRPVVIQP 1781
+ V + V + ++ISGRP+VI P
Sbjct: 337 LITRVASKVPTLVIVISGRPLVIDP 411
Score = 72.5 bits (152), Expect(3) = 3e-062
Identities = 26/34 (76%), Positives = 29/34 (85%)
Frame = +3 / +3
Query: 1395 LPLPKKAARILVAGSHADNLGYQCGGWTIEWQGD 1496
LPL K A RILVAG+HAD++GYQCGGWTI W GD
Sbjct: 24 LPLAKNAKRILVAGTHADDIGYQCGGWTIAWHGD 125
Score = 61.6 bits (128), Expect(3) = 1e-027
Identities = 28/49 (57%), Positives = 33/49 (67%)
Frame = -2 / -2
Query: 1939 ASP*FIGSWSTDLNHVRGSFPVNP*SPNSTSVTPWPSVPGSQAATTASM 1793
ASP FIG+ S +LNHV G+ P SP SV P PS+PGS AAT AS+
Sbjct: 572 ASPIFIGN*SENLNHVTGNVPTKSWSPKMQSVIPLPSLPGSHAATRASI 426
Score = 49.2 bits (101), Expect(3) = 1e-027
Identities = 31/78 (39%), Positives = 39/78 (50%)
Frame = -2 / -2
Query: 1780 GCITTGRPLMSTVTHRTAAHTVCTVLGPGSGIVRFMLSPLVSVYGCSPTATIAYEKPPLL 1601
G IT G P M+ + T T P GI+R +LSP S G SPT T A E PP+
Sbjct: 410 GSITKGLPDMTITSVGTLLATRVIRSEPLKGILRSVLSPNHSA*GTSPTTTTA*ENPPVS 231
Query: 1600 TNSASGLSANTTSVDGST 1547
++ G + TTSV T
Sbjct: 230 MIASVGHCSCTTSVSTPT 177
Score = 46.4 bits (95), Expect(3) = 1e-027
Identities = 19/31 (61%), Positives = 21/31 (67%)
Frame = -2 / -3
Query: 1489 CHSIVHPPHWYPRLSAWLPATRILAAFLGRG 1397
C +IVHPPH YP SA +PAT I AFL G
Sbjct: 118 CQAIVHPPH*YPISSACVPATSIRFAFLANG 26
>gb|BE439135.1|BE439135 CDO465_WHE2G0092F ITEC CDO Oat cDNA Library Avena sativa cDNA clone
CDO465_WHE2G0092, mRNA sequence
Length = 284
Score = 98.2 bits (208), Expect = 5e-021
Identities = 38/56 (67%), Positives = 43/56 (76%)
Frame = +3 / -3
Query: 1860 GDYGFTGKLPRTWFRSVDQLPMNYGDAHYDPLFPLGFGLTTQGKMYQN*KKAERGE 2027
GDYGF+GKL RTW +SVDQLPMN GD HYDPLFP GFGLTT+ K *+ + GE
Sbjct: 282 GDYGFSGKLARTWLKSVDQLPMNVGDKHYDPLFPFGFGLTTEAKK*MQ*EIIKMGE 115
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 17,713,296
Number of Sequences: 8143
Number of extensions: 223035
Number of successful extensions: 1270
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 46
effective length of database: 1,192,909
effective search space used: 831457573
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)