TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2430946.2.2
(3838 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815813.1|CN815813 HRO4510_G06_M12ZS5 Lib AA071E1X Aven... 198 7e-051
gb|CN819884.1|CN819884 HRO4407_C09_F17ZS5 Lib AA069E1X Aven... 195 5e-050
gb|CN819594.1|CN819594 HRO4401_H12_O23ZS5 Lib AA069E1X Aven... 145 4e-035
gb|CN815647.1|CN815647 HRO4514_A01_A02ZS5 Lib AA071E1X Aven... 100 1e-021
>gb|CN815813.1|CN815813 HRO4510_G06_M12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4510_G06_M12, mRNA sequence
Length = 521
Score = 198 bits (426), Expect = 7e-051
Identities = 73/96 (76%), Positives = 92/96 (95%)
Frame = -3 / +2
Query: 755 KWTSLLIPPTTVLVINLVGMVAGISYAINSGYQSWGPLFGKLFFSIWVILHLYPFLKGLM 576
+WT+LLIPPTT+++IN+VG+VAG+S A+N+GY SWGPLFGKLFFS WVI+HLYPFLKGLM
Sbjct: 11 QWTTLLIPPTTLIIINMVGIVAGVSDAVNNGYGSWGPLFGKLFFSFWVIVHLYPFLKGLM 190
Query: 575 GRQNRTPTIVIVWSILLASIFSLLWVKIDPFISPTQ 468
GRQNRTPTIV++WS+LLASIFSL+WV+IDPFI+ ++
Sbjct: 191 GRQNRTPTIVVLWSVLLASIFSLVWVRIDPFIAKSK 298
Score = 143 bits (307), Expect = 2e-034
Identities = 60/91 (65%), Positives = 72/91 (79%)
Frame = +1 / -3
Query: 481 MKGSIFTHSKEKIDARRMDQTMTIVGVRFCLPMRPLRKG*RWRITQIEKNSFPKSGPQDW 660
MKGSI T ++EK++A R DQ+ T+VGVRFCLPM PLR G*RW +TQ EK SFPK GP D
Sbjct: 285 MKGSILTQTREKMEASRTDQSTTMVGVRFCLPMSPLRNG*RWTMTQKEKKSFPKRGPHDP 106
Query: 661 *PLLMAYEIPATIPTRLMTRTVVGGMSKLVH 753
*PLL A E PAT+PT LM +VVGG+S++VH
Sbjct: 105 *PLLTASETPATMPTMLMMMSVVGGISRVVH 13
>gb|CN819884.1|CN819884 HRO4407_C09_F17ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4407_C09_F17, mRNA
sequence
Length = 676
Score = 195 bits (420), Expect = 5e-050
Identities = 76/88 (86%), Positives = 85/88 (96%)
Frame = -3 / +1
Query: 746 SLLIPPTTVLVINLVGMVAGISYAINSGYQSWGPLFGKLFFSIWVILHLYPFLKGLMGRQ 567
+LLIPPTT+L+IN+VG+VAG SYAINSGYQSWGPLFGKLFF+ WVI+HLYPFLKGLMGR
Sbjct: 13 TLLIPPTTILIINMVGVVAGTSYAINSGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRT 192
Query: 566 NRTPTIVIVWSILLASIFSLLWVKIDPF 483
NRTPTIVIVW+ILLASIFSLLWV+IDPF
Sbjct: 193 NRTPTIVIVWAILLASIFSLLWVRIDPF 276
Score = 139 bits (298), Expect = 3e-033
Identities = 61/86 (70%), Positives = 68/86 (79%)
Frame = +1 / -3
Query: 481 MKGSIFTHSKEKIDARRMDQTMTIVGVRFCLPMRPLRKG*RWRITQIEKNSFPKSGPQDW 660
+ GSI THSKEKI+ARR+ QTMTIVGVRF LP+RPLR G + ITQ K SFP SGP DW
Sbjct: 278 VNGSIRTHSKEKIEARRIAQTMTIVGVRFVLPIRPLRNGYK*TITQKAKKSFPNSGPHDW 99
Query: 661 *PLLMAYEIPATIPTRLMTRTVVGGM 738
*PLLMA E+PAT PT LM + VVGGM
Sbjct: 98 *PLLMA*EVPATTPTMLMIKIVVGGM 21
Score = 108 bits (230), Expect = 7e-024
Identities = 44/89 (49%), Positives = 67/89 (75%)
Frame = +3 / -2
Query: 477 GDERIDLHPQQGEDRCKKDGPDNDDCWCAVLPSHETLEEGVEMEDHPDREEQLSKERTPG 656
G E I+ +PQQGEDR ++D PD+D+ CAV P+H+TLEE V + +HP+ +E+LS++R P
Sbjct: 282 GSEWINTNPQQGEDRSEEDSPDDDNRRCAVCPAHKTLEEWV*VNNHPEGKEELSEQRPP* 103
Query: 657 LVATVNGIRNSCHHSDQVNDKNSGRRNEQ 743
LV TV+GI + +++D VND+N R+E+
Sbjct: 102 LVTTVDGIGGASNNTDHVNDQNCCWRDEK 16
>gb|CN819594.1|CN819594 HRO4401_H12_O23ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4401_H12_O23, mRNA
sequence
Length = 620
Score = 145 bits (312), Expect = 4e-035
Identities = 56/62 (90%), Positives = 60/62 (96%)
Frame = -3 / +2
Query: 668 SGYQSWGPLFGKLFFSIWVILHLYPFLKGLMGRQNRTPTIVIVWSILLASIFSLLWVKID 489
SGYQSWGPLFGKLFF+ WVI+HLYPFLKGLMGR NRTPTIVIVW+ILLASIFSLLWV+ID
Sbjct: 2 SGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRTNRTPTIVIVWAILLASIFSLLWVRID 181
Query: 488 PF 483
PF
Sbjct: 182 PF 187
Score = 106 bits (226), Expect = 3e-023
Identities = 46/63 (73%), Positives = 51/63 (80%)
Frame = +1 / -3
Query: 481 MKGSIFTHSKEKIDARRMDQTMTIVGVRFCLPMRPLRKG*RWRITQIEKNSFPKSGPQDW 660
+ GSI THSKEKI+ARR+ QTMTIVGVRF LP+RPLR G + ITQ K SFPKSGP DW
Sbjct: 189 VNGSIRTHSKEKIEARRIAQTMTIVGVRFVLPIRPLRNGYK*TITQKAKKSFPKSGPHDW 10
Query: 661 *PL 669
*PL
Sbjct: 9 *PL 1
>gb|CN815647.1|CN815647 HRO4514_A01_A02ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4514_A01_A02, mRNA sequence
Length = 474
Score = 100 bits (214), Expect = 1e-021
Identities = 39/48 (81%), Positives = 46/48 (95%)
Frame = -3 / +3
Query: 614 VILHLYPFLKGLMGRQNRTPTIVIVWSILLASIFSLLWVKIDPFISPT 471
VI+HLYPFLKGL+GRQNRTPTIVIVWSILLASI SLLWV+++PF++ T
Sbjct: 12 VIVHLYPFLKGLLGRQNRTPTIVIVWSILLASIISLLWVRVNPFLAKT 155
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 46,489,106
Number of Sequences: 8143
Number of extensions: 711216
Number of successful extensions: 3096
Number of sequences better than 1.0e-020: 4
length of database: 1,567,487
effective HSP length: 47
effective length of database: 1,184,766
effective search space used: 1458446946
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)