TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2419536.2.1
(1366 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815034.1|CN815034 HRO4505_B06_C11ZS5 Lib AA071E1X Aven... 147 2e-046
gb|CN816101.1|CN816101 HRO4517_A05_A09ZS5 Lib AA071E1X Aven... 135 1e-044
gb|CN814875.1|CN814875 HRO4503_D10_H19ZS5 Lib AA071E1X Aven... 156 6e-039
gb|CN816873.1|CN816873 HRO4525_G05_M09ZS5 Lib AA071E1X Aven... 105 2e-036
gb|CN821053.1|CN821053 HRO4427_A04_B07ZS5 Lib AA069E1X Aven... 101 7e-033
gb|CN815850.1|CN815850 HRO4511_B09_D17ZS5 Lib AA071E1X Aven... 98 2e-031
gb|CN814789.1|CN814789 HRO4502_E05_I10ZS5 Lib AA071E1X Aven... 82 5e-027
gb|CN814955.1|CN814955 HRO4504_C09_F18ZS5 Lib AA071E1X Aven... 101 2e-022
>gb|CN815034.1|CN815034 HRO4505_B06_C11ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4505_B06_C11, mRNA sequence
Length = 629
Score = 147 bits (315), Expect(2) = 2e-046
Identities = 59/99 (59%), Positives = 74/99 (74%)
Frame = +2 / +1
Query: 659 DVGGGIGTAAQAISKAFPHVKCSVLDLAHVVAKAPTHTDVQFIAGDMFESIPPADAVLLK 838
D GGG T A+AI+KA PH+KC+VLDL V+ K P+ V ++AGD+F S+P + AVLLK
Sbjct: 7 DCGGGD*TTARAITKAHPHIKCTVLDLPKVIEKVPSDGIVNYVAGDLFHSVPKSQAVLLK 186
Query: 839 SVLHDWDHDDCVKILKNCKKAIPPREAGGKVIIINMVVG 955
VLH W DDCVKIL C+ AIP RE GGKVIII+++VG
Sbjct: 187 LVLHHWSDDDCVKILSQCRSAIPSREEGGKVIIIDILVG 303
Score = 92.7 bits (196), Expect(2) = 5e-021
Identities = 48/99 (48%), Positives = 58/99 (58%)
Frame = -2 / -1
Query: 954 PTTMFIIITFPPASLGGIAFLQFFSIFTQSSWSQSCKTDFNSTASAGGMLSNMSPAINCT 775
PT + ++ITFPP+S G+A L IFTQSS Q C T ++TA G N SPA T
Sbjct: 302 PTRISMMITFPPSSREGMALLH*DKIFTQSSSLQWCSTSLSNTA*DFGTEWNKSPAT*LT 123
Query: 774 SV*VGAFATTWARSSTLHLTCGNAFEMAWAAVPMPPPTS 658
G F+ T+ RSST+HL CG A MA A V PPP S
Sbjct: 122 IPSDGTFSITFGRSSTVHLMCGWALVMALAVVQSPPPQS 6
Score = 54.7 bits (113), Expect(2) = 2e-046
Identities = 17/36 (47%), Positives = 27/36 (75%)
Frame = +2 / +1
Query: 1025 GMERDEQEWSKIFSEAGYSDYRIIPVLGVRSIIEVY 1132
G +RDE +W ++F +AG+SDY+++ LG R + EVY
Sbjct: 373 GRQRDENDWRQLFMKAGFSDYKVVKKLGARGVFEVY 480
Score = 24.0 bits (46), Expect(2) = 5e-021
Identities = 18/38 (47%), Positives = 19/38 (50%)
Frame = -2 / -1
Query: 1131 *TSIIDRTPKTGIIL*SLYPASEKILLHSCSSRSMPLM 1018
*TS R P L*SL PA K S SSR PL+
Sbjct: 479 *TSKTPRAPNFLTTL*SLNPAFMKS*RQSFSSR*RPLV 366
>gb|CN816101.1|CN816101 HRO4517_A05_A09ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4517_A05_A09, mRNA sequence
Length = 652
Score = 135 bits (290), Expect(2) = 1e-044
Identities = 51/84 (60%), Positives = 66/84 (78%)
Frame = +2 / +3
Query: 701 KAFPHVKCSVLDLAHVVAKAPTHTDVQFIAGDMFESIPPADAVLLKSVLHDWDHDDCVKI 880
KA+PH+KC+VLDL V+ KAP H + ++AGD+F ++PP+ AV+LK VLH W +DCVKI
Sbjct: 15 KAYPHLKCTVLDLPKVIEKAPPHDVINYVAGDLFHTVPPSQAVMLKLVLHFWSDEDCVKI 194
Query: 881 LKNCKKAIPPREAGGKVIIINMVV 952
L C+KAIP RE GGKVIII +VV
Sbjct: 195 LAQCRKAIPSREEGGKVIIIEIVV 266
Score = 82.6 bits (174), Expect(2) = 3e-021
Identities = 41/81 (50%), Positives = 52/81 (64%)
Frame = -2 / -1
Query: 951 TTMFIIITFPPASLGGIAFLQFFSIFTQSSWSQSCKTDFNSTASAGGMLSNMSPAINCTS 772
TT+ +IITFPP+S G+AFL + IFTQSS Q T + TA GG + SPA +
Sbjct: 265 TTISMIITFPPSSREGMAFLHWARIFTQSSSLQK*STSLSITA*DGGTVWKRSPAT*LMT 86
Query: 771 V*VGAFATTWARSSTLHLTCG 709
* GAF+ T+ RSST+HL CG
Sbjct: 85 S*GGAFSITFGRSSTVHLRCG 23
Score = 60.2 bits (125), Expect(2) = 1e-044
Identities = 21/38 (55%), Positives = 31/38 (81%)
Frame = +2 / +3
Query: 1025 GMERDEQEWSKIFSEAGYSDYRIIPVLGVRSIIEVYP* 1138
G +RDE+ W ++F +AG++DY+I+ LG RS+IEVYP*
Sbjct: 339 GGQRDEKHWRELFMQAGFTDYKIVKKLGARSVIEVYP* 452
Score = 34.5 bits (69), Expect(2) = 3e-021
Identities = 22/52 (42%), Positives = 25/52 (48%)
Frame = -2 / -1
Query: 1173 HATIYEPSYTNGYG*TSIIDRTPKTGIIL*SLYPASEKILLHSCSSRSMPLM 1018
H P T YG*TS+ DR P IL*S+ PA SSR PL+
Sbjct: 487 HFLYLYP*KTPSYG*TSMTDRAPSFFTIL*SVNPACINSSRQCFSSR*PPLV 332
>gb|CN814875.1|CN814875 HRO4503_D10_H19ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4503_D10_H19, mRNA sequence
Length = 846
Score = 156 bits (336), Expect = 6e-039
Identities = 60/92 (65%), Positives = 80/92 (86%)
Frame = +2 / +1
Query: 863 DDCVKILKNCKKAIPPREAGGKVIIINMVVGAGPSDMKHKEMQAIFDVYIMFINGMERDE 1042
+DCVKILK CK+AIP R+AGGKVII++MVVG+GP+D+ +E Q FD++IM++ G+ER+E
Sbjct: 16 EDCVKILKKCKEAIPSRDAGGKVIIVDMVVGSGPNDIVTRETQVFFDLFIMYLEGIEREE 195
Query: 1043 QEWSKIFSEAGYSDYRIIPVLGVRSIIEVYP* 1138
EW KIF EAG+S+Y+II VLGVRS+IE+YP*
Sbjct: 196 FEWKKIFMEAGFSEYKIISVLGVRSVIELYP* 291
Score = 112 bits (239), Expect = 1e-025
Identities = 56/91 (61%), Positives = 61/91 (67%)
Frame = -2 / -2
Query: 1134 G*TSIIDRTPKTGIIL*SLYPASEKILLHSCSSRSMPLMNMI*TSNMACISLCFMSDGPA 955
G*+SI D TP T +IL SL PAS I HS SSRS+P MI S C+S MS GP
Sbjct: 287 G*SSITDLTPSTDMILYSLNPASMNIFFHSNSSRSIPSRYMIKRSKKTCVSRVTMSFGPD 108
Query: 954 PTTMFIIITFPPASLGGIAFLQFFSIFTQSS 862
PTT+ IITFPPASL GIA L FFSI TQSS
Sbjct: 107 PTTISTIITFPPASLEGIASLHFFSILTQSS 15
>gb|CN816873.1|CN816873 HRO4525_G05_M09ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4525_G05_M09, mRNA sequence
Length = 662
Score = 105 bits (223), Expect(3) = 2e-036
Identities = 41/70 (58%), Positives = 52/70 (74%)
Frame = +2 / +2
Query: 746 VVAKAPTHTDVQFIAGDMFESIPPADAVLLKSVLHDWDHDDCVKILKNCKKAIPPREAGG 925
V+ K P+ V ++AGD+F S+P + AVLLK VLH W DDCVKIL C+ AIP RE GG
Sbjct: 41 VIEKVPSDGIVNYVAGDLFHSVPKSQAVLLKLVLHHWSDDDCVKILSQCRSAIPSREEGG 220
Query: 926 KVIIINMVVG 955
KVIII+++VG
Sbjct: 221 KVIIIDILVG 250
Score = 54.7 bits (113), Expect(3) = 2e-036
Identities = 17/36 (47%), Positives = 27/36 (75%)
Frame = +2 / +2
Query: 1025 GMERDEQEWSKIFSEAGYSDYRIIPVLGVRSIIEVY 1132
G +RDE +W ++F +AG+SDY+++ LG R + EVY
Sbjct: 320 GRQRDENDWRQLFMKAGFSDYKVVKKLGARGVFEVY 427
Score = 26.3 bits (51), Expect(3) = 2e-036
Identities = 8/10 (80%), Positives = 10/10 (100%)
Frame = +2 / +3
Query: 710 PHVKCSVLDL 739
PH+KC+VLDL
Sbjct: 6 PHIKCTVLDL 35
>gb|CN821053.1|CN821053 HRO4427_A04_B07ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4427_A04_B07, mRNA
sequence
Length = 644
Score = 101 bits (215), Expect(2) = 7e-033
Identities = 40/72 (55%), Positives = 52/72 (72%)
Frame = +2 / +3
Query: 740 AHVVAKAPTHTDVQFIAGDMFESIPPADAVLLKSVLHDWDHDDCVKILKNCKKAIPPREA 919
A + K P+ V ++AGD+F S+P + AVLLK VLH W +DCVKIL C+ AIP RE
Sbjct: 3 ARGIDKVPSDGIVNYVAGDLFHSVPKSQAVLLKLVLHHWSDEDCVKILSQCRNAIPSREK 182
Query: 920 GGKVIIINMVVG 955
GGKVIII+++VG
Sbjct: 183 GGKVIIIDILVG 218
Score = 55.1 bits (114), Expect(2) = 7e-033
Identities = 18/36 (50%), Positives = 27/36 (75%)
Frame = +2 / +3
Query: 1025 GMERDEQEWSKIFSEAGYSDYRIIPVLGVRSIIEVY 1132
G +RDE +W ++F +AG+SDY+I+ LG R + EVY
Sbjct: 288 GRQRDENDWRQLFMKAGFSDYKIVKKLGARGVFEVY 395
>gb|CN815850.1|CN815850 HRO4511_B09_D17ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4511_B09_D17, mRNA sequence
Length = 621
Score = 97.7 bits (207), Expect(2) = 2e-031
Identities = 38/65 (58%), Positives = 49/65 (75%)
Frame = +2 / +2
Query: 761 PTHTDVQFIAGDMFESIPPADAVLLKSVLHDWDHDDCVKILKNCKKAIPPREAGGKVIII 940
P+ V ++AGD+F S+P + AV+LK VLH W +DCVKIL CK AIP RE GGKVIII
Sbjct: 2 PSDGIVYYVAGDLFHSVPKSQAVMLKLVLHRWSDEDCVKILSQCKSAIPSREEGGKVIII 181
Query: 941 NMVVG 955
+++VG
Sbjct: 182 DILVG 196
Score = 53.8 bits (111), Expect(2) = 2e-031
Identities = 17/36 (47%), Positives = 27/36 (75%)
Frame = +2 / +2
Query: 1025 GMERDEQEWSKIFSEAGYSDYRIIPVLGVRSIIEVY 1132
G +RDE +W ++F +AG+SDY+I+ +G R + EVY
Sbjct: 266 GRQRDENDWRQLFMKAGFSDYKIVKKVGARGVFEVY 373
>gb|CN814789.1|CN814789 HRO4502_E05_I10ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4502_E05_I10, mRNA sequence
Length = 596
Score = 81.7 bits (172), Expect(2) = 5e-027
Identities = 32/50 (64%), Positives = 39/50 (78%)
Frame = +2 / -3
Query: 806 SIPPADAVLLKSVLHDWDHDDCVKILKNCKKAIPPREAGGKVIIINMVVG 955
S+P + AVLLK VLH W +DCVKIL C+ AIP RE GGKVIII+++VG
Sbjct: 591 SVPKSQAVLLKLVLHHWSDEDCVKILSQCRNAIPSREKGGKVIIIDILVG 442
Score = 55.1 bits (114), Expect(2) = 5e-027
Identities = 18/36 (50%), Positives = 27/36 (75%)
Frame = +2 / -3
Query: 1025 GMERDEQEWSKIFSEAGYSDYRIIPVLGVRSIIEVY 1132
G +RDE +W ++F +AG+SDY+I+ LG R + EVY
Sbjct: 372 GRQRDENDWRQLFMKAGFSDYKIVKKLGARGVFEVY 265
>gb|CN814955.1|CN814955 HRO4504_C09_F18ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4504_C09_F18, mRNA sequence
Length = 648
Score = 101 bits (216), Expect = 2e-022
Identities = 39/71 (54%), Positives = 51/71 (71%)
Frame = +2 / +1
Query: 776 VQFIAGDMFESIPPADAVLLKSVLHDWDHDDCVKILKNCKKAIPPREAGGKVIIINMVVG 955
V ++AGDMF+ +PPA AVL+K VLH W +DCVKIL CKKA+P RE GGKVI+I++VV
Sbjct: 16 VNYVAGDMFKLVPPAQAVLVKLVLHHWSDEDCVKILAQCKKAVPSREEGGKVIVIDIVVD 195
Query: 956 AGPSDMKHKEM 988
+ E+
Sbjct: 196 SSSGPTHEAEL 228
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 16,711,026
Number of Sequences: 8143
Number of extensions: 268216
Number of successful extensions: 1949
Number of sequences better than 1.0e-020: 9
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 495769950
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)