TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2419471.2.6
(1648 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816713.1|CN816713 HRO4523_H09_P17ZS5 Lib AA071E1X Aven... 179 2e-062
gb|BE439203.1|BE439203 CDO1421_WHE03E02R ITEC CDO Oat cDNA ... 185 1e-047
gb|CN815566.1|CN815566 HRO4513_B02_C03ZS5 Lib AA071E1X Aven... 94 6e-037
gb|CN817098.1|CN817098 HRO4528_B12_D24ZS5 Lib AA071E1X Aven... 89 4e-035
gb|CN821529.1|CN821529 HRO4426_D02_G04ZS5 Lib AA069E1X Aven... 77 4e-027
>gb|CN816713.1|CN816713 HRO4523_H09_P17ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4523_H09_P17, mRNA sequence
Length = 608
Score = 179 bits (385), Expect(2) = 2e-062
Identities = 75/83 (90%), Positives = 80/83 (96%)
Frame = +3 / +2
Query: 1014 KVDRSGAYVARQAAKSIVASGLARRAIVQVSYAIGVPEPLSVFVDTYGTGAIPDKEILKI 1193
KVDRSGAY+ARQAAKSIVASGLARR IVQ+SYAIGVPEPLSVFVD+YGTG IPD+EILK+
Sbjct: 17 KVDRSGAYIARQAAKSIVASGLARRVIVQISYAIGVPEPLSVFVDSYGTGKIPDREILKL 196
Query: 1194 VKENFDFRPGMIIINLDLKKGGN 1262
VKENFDFRPGMI INLDLKKGGN
Sbjct: 197 VKENFDFRPGMISINLDLKKGGN 265
Score = 84.5 bits (178), Expect(3) = 2e-030
Identities = 37/52 (71%), Positives = 42/52 (80%)
Frame = -1 / -3
Query: 1234 MMIMPGLKSKFSLTILRISLSGIAPVPYVSTNTERGSGTPMA*DTWTMARRA 1079
M+I+PGLKSKFS T RISLSGI PVP STNT++GSGTPMA + TM RRA
Sbjct: 237 MLIIPGLKSKFSFTSFRISLSGILPVP*ESTNTDKGSGTPMAYEICTMTRRA 82
Score = 76.7 bits (161), Expect(2) = 2e-062
Identities = 30/39 (76%), Positives = 33/39 (84%)
Frame = +3 / +2
Query: 1251 KGGNGRYLKTAAYGHFGRDDPDFTWEVVKPLKSEKPSA* 1367
K G R++KTAAYGHFGRDD DFTWEVVKPLK +K SA*
Sbjct: 251 KKGGNRFIKTAAYGHFGRDDADFTWEVVKPLKFDKTSA* 367
Score = 44.1 bits (90), Expect(3) = 2e-030
Identities = 18/35 (51%), Positives = 24/35 (68%)
Frame = -3 / -2
Query: 1376 KAALGRRFLRLEWLHHLPGEVRVVPSKVAVGRRLE 1272
K L RR ++LE LH+LPGEV ++ KV VG L+
Sbjct: 376 KQLLSRRLVKLERLHNLPGEVSIITPKVTVGSGLD 272
Score = 37.7 bits (76), Expect(3) = 2e-030
Identities = 12/17 (70%), Positives = 13/17 (76%)
Frame = -2 / -1
Query: 1062 CSWQPALRHRLRCGQPW 1012
CSWQPA + R CGQPW
Sbjct: 65 CSWQPAWQCRHHCGQPW 15
>gb|BE439203.1|BE439203 CDO1421_WHE03E02R ITEC CDO Oat cDNA Library Avena sativa cDNA clone
CDO1421_WHE03E02, mRNA sequence
Length = 275
Score = 185 bits (399), Expect = 1e-047
Identities = 75/82 (91%), Positives = 77/82 (93%)
Frame = +3 / +3
Query: 1122 PEPLSVFVDTYGTGAIPDKEILKIVKENFDFRPGMIIINLDLKKGGNGRYLKTAAYGHFG 1301
PEPLSVFVDTYGTG IPDK+IL IVKENFDFRPGMIIINLDLK+GGNGRYLKTAAYGHFG
Sbjct: 3 PEPLSVFVDTYGTGRIPDKDILNIVKENFDFRPGMIIINLDLKRGGNGRYLKTAAYGHFG 182
Query: 1302 RDDPDFTWEVVKPLKSEKPSA* 1367
RD PDFTWEVVKPLK E PSA*
Sbjct: 183 RDGPDFTWEVVKPLKWENPSA* 248
Score = 111 bits (236), Expect = 5e-025
Identities = 46/69 (66%), Positives = 51/69 (73%)
Frame = +2 / +2
Query: 1166 DPRQGDPQDCQGELRFQAWHDHHQP*PQERRQRALPQDGGLRPLWKGRP*LHLGGGEATQ 1345
DP QG PQ CQGEL QA HDHHQP P+E + A+PQDGG+ LWKG LHLGGGEA Q
Sbjct: 47 DP*QGYPQHCQGEL*LQARHDHHQPRPEEGWEWAVPQDGGVWALWKGWARLHLGGGEAPQ 226
Query: 1346 VGETFCLRR 1372
VGE FCL +
Sbjct: 227 VGEPFCLSK 253
Score = 82.2 bits (173), Expect(2) = 5e-032
Identities = 35/38 (92%), Positives = 36/38 (94%)
Frame = -1 / -2
Query: 1234 MMIMPGLKSKFSLTILRISLSGIAPVPYVSTNTERGSG 1121
MMIMPGLKSKFSLT+LRISLSGI PVPYVSTNTE GSG
Sbjct: 115 MMIMPGLKSKFSLTMLRISLSGILPVPYVSTNTESGSG 2
Score = 71.6 bits (150), Expect(2) = 5e-032
Identities = 36/53 (67%), Positives = 40/53 (75%)
Frame = -2 / -3
Query: 1368 LRQKVSPT*VASPPPR*SQGRPFQSGRRPPS*GSARCRLS*GQG***SCQA*N 1210
LRQK SPT* ASPPPR*S PFQS PPS*G+A S G+G***SC+A*+
Sbjct: 249 LRQKGSPT*GASPPPR*SLAHPFQSAHTPPS*GTAHSHPSSGRG***SCRA*S 91
>gb|CN815566.1|CN815566 HRO4513_B02_C03ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4513_B02_C03, mRNA sequence
Length = 656
Score = 93.6 bits (198), Expect(2) = 6e-037
Identities = 37/42 (88%), Positives = 40/42 (95%)
Frame = +3 / +1
Query: 1137 VFVDTYGTGAIPDKEILKIVKENFDFRPGMIIINLDLKKGGN 1262
VFVD+YGTG IPD+EILK+VKENFDFRPGMI INLDLKKGGN
Sbjct: 10 VFVDSYGTGKIPDREILKLVKENFDFRPGMISINLDLKKGGN 135
Score = 76.7 bits (161), Expect(2) = 6e-037
Identities = 30/39 (76%), Positives = 33/39 (84%)
Frame = +3 / +1
Query: 1251 KGGNGRYLKTAAYGHFGRDDPDFTWEVVKPLKSEKPSA* 1367
K G R++KTAAYGHFGRDD DFTWEVVKPLK +K SA*
Sbjct: 121 KKGGNRFIKTAAYGHFGRDDADFTWEVVKPLKFDKTSA* 237
Score = 57.0 bits (118), Expect(3) = 5e-021
Identities = 25/33 (75%), Positives = 27/33 (81%)
Frame = -1 / -1
Query: 1234 MMIMPGLKSKFSLTILRISLSGIAPVPYVSTNT 1136
M+I+PGLKSKFS T RISLSGI PVP STNT
Sbjct: 107 MLIIPGLKSKFSFTSFRISLSGILPVP*ESTNT 9
Score = 44.1 bits (90), Expect(3) = 5e-021
Identities = 18/35 (51%), Positives = 24/35 (68%)
Frame = -3 / -3
Query: 1376 KAALGRRFLRLEWLHHLPGEVRVVPSKVAVGRRLE 1272
K L RR ++LE LH+LPGEV ++ KV VG L+
Sbjct: 246 KQLLSRRLVKLERLHNLPGEVSIITPKVTVGSGLD 142
Score = 33.1 bits (66), Expect(3) = 5e-021
Identities = 11/19 (57%), Positives = 15/19 (78%)
Frame = -3 / -3
Query: 1265 PVAAFLEVKVDDDHARPEI 1209
P+A L+VKVD DH RP++
Sbjct: 138 PIATLLQVKVDADHPRPKV 82
>gb|CN817098.1|CN817098 HRO4528_B12_D24ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4528_B12_D24, mRNA sequence
Length = 718
Score = 89.0 bits (188), Expect(2) = 4e-035
Identities = 35/39 (89%), Positives = 37/39 (94%)
Frame = +3 / +3
Query: 1146 DTYGTGAIPDKEILKIVKENFDFRPGMIIINLDLKKGGN 1262
DTYGTG IPD+EILK+VKENFDFRPGMI INLDLKKGGN
Sbjct: 12 DTYGTGKIPDREILKLVKENFDFRPGMISINLDLKKGGN 128
Score = 75.3 bits (158), Expect(2) = 4e-035
Identities = 27/38 (71%), Positives = 33/38 (86%)
Frame = +3 / +3
Query: 1251 KGGNGRYLKTAAYGHFGRDDPDFTWEVVKPLKSEKPSA 1364
K G R++KTAAYGHFGR+DPDFTWEVVKPLK +K ++
Sbjct: 114 KKGGNRFIKTAAYGHFGREDPDFTWEVVKPLKFDKAAS 227
>gb|CN821529.1|CN821529 HRO4426_D02_G04ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4426_D02_G04, mRNA
sequence
Length = 515
Score = 76.7 bits (161), Expect(2) = 4e-027
Identities = 30/39 (76%), Positives = 33/39 (84%)
Frame = +3 / +1
Query: 1251 KGGNGRYLKTAAYGHFGRDDPDFTWEVVKPLKSEKPSA* 1367
K G R++KTAAYGHFGRDD DFTWEVVKPLK +K SA*
Sbjct: 64 KKGGNRFIKTAAYGHFGRDDADFTWEVVKPLKFDKTSA* 180
Score = 60.6 bits (126), Expect(2) = 4e-027
Identities = 24/26 (92%), Positives = 25/26 (96%)
Frame = +3 / +1
Query: 1185 LKIVKENFDFRPGMIIINLDLKKGGN 1262
LK+VKENFDFRPGMI INLDLKKGGN
Sbjct: 1 LKLVKENFDFRPGMISINLDLKKGGN 78
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 18,821,360
Number of Sequences: 8143
Number of extensions: 311136
Number of successful extensions: 2413
Number of sequences better than 1.0e-020: 6
length of database: 1,567,487
effective HSP length: 45
effective length of database: 1,201,052
effective search space used: 604129156
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)