TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2419417.2.5
(733 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN820277.1|CN820277 HRO4417_E10_I19ZS5 Lib AA069E1X Aven... 183 2e-047
gb|CN820708.1|CN820708 HRO4416_F04_L08ZS5 Lib AA069E1X Aven... 97 1e-037
gb|CN818518.1|CN818518 HRO4459_F04_K07ZS5 Lib AA070E1X Aven... 97 1e-037
gb|CN820950.1|CN820950 HRO4421_G11_M21ZS5 Lib AA069E1X Aven... 68 6e-022
gb|CN815884.1|CN815884 HRO4511_E10_J19ZS5 Lib AA071E1X Aven... 68 6e-022
gb|CN821160.1|CN821160 HRO4428_B09_D18ZS5 Lib AA069E1X Aven... 68 6e-022
gb|CN821349.1|CN821349 HRO4424_C10_F20ZS5 Lib AA069E1X Aven... 68 6e-022
emb|X78433.1|ASLBDG A.sativa L. mRNA for beta-D-glucosidase 82 6e-021
>gb|CN820277.1|CN820277 HRO4417_E10_I19ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4417_E10_I19, mRNA
sequence
Length = 789
Score = 183 bits (395), Expect = 2e-047
Identities = 64/112 (57%), Positives = 91/112 (81%)
Frame = +2 / +1
Query: 8 KLMNYVKERYNSPTVYITENGMDDGNSPFTSIQDALKDSKRIKYHNGYLNNVAASIKEDG 187
KLM +VKE+Y +P V+ITENGMDD N+ F+ ++ L+D KRI+YHN Y++++ +I+++G
Sbjct: 211 KLMKHVKEKYGNPPVFITENGMDDANNRFSRLETVLQDDKRIQYHNDYMSSLLDAIRKEG 390
Query: 188 CDVRGYFAWSLLDNWEWAAGYTSRFGLYFVDYKDNLKRYPKNSVQWFKTLLS 343
C+V+GYF WSLLDNWEW +GYT RFGLY++DY +NL R PK SV+WF +L+
Sbjct: 391 CNVQGYFVWSLLDNWEWNSGYTVRFGLYYIDYNNNLTRIPKASVEWFSQVLA 546
>gb|CN820708.1|CN820708 HRO4416_F04_L08ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4416_F04_L08, mRNA
sequence
Length = 780
Score = 97.3 bits (206), Expect(2) = 1e-037
Identities = 34/55 (61%), Positives = 42/55 (76%)
Frame = +2 / +2
Query: 179 EDGCDVRGYFAWSLLDNWEWAAGYTSRFGLYFVDYKDNLKRYPKNSVQWFKTLLS 343
+DG DVRGYFAWS LDN+EWA G+T RFG+ +VDYK+ L R+PK S WF L+
Sbjct: 197 KDGADVRGYFAWSFLDNFEWAMGFTKRFGIVYVDYKNGLSRHPKASAMWFSRFLN 361
Score = 74.4 bits (156), Expect(2) = 1e-037
Identities = 26/59 (44%), Positives = 42/59 (71%)
Frame = +2 / +2
Query: 5 RKLMNYVKERYNSPTVYITENGMDDGNSPFTSIQDALKDSKRIKYHNGYLNNVAASIKE 181
RK++N+V ++Y +P +YITENGMDD + P ++ L D+KR+ + GY+ VA +IK+
Sbjct: 26 RKMINHVAKKYGNPIIYITENGMDDEDDPSATLDQVLNDTKRVGFFKGYVGAVAEAIKD 202
>gb|CN818518.1|CN818518 HRO4459_F04_K07ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4459_F04_K07, mRNA sequence
Length = 763
Score = 97.3 bits (206), Expect(2) = 1e-037
Identities = 34/55 (61%), Positives = 42/55 (76%)
Frame = +2 / +1
Query: 179 EDGCDVRGYFAWSLLDNWEWAAGYTSRFGLYFVDYKDNLKRYPKNSVQWFKTLLS 343
+DG DVRGYFAWS LDN+EWA G+T RFG+ +VDYK+ L R+PK S WF L+
Sbjct: 202 KDGADVRGYFAWSFLDNFEWAMGFTKRFGIVYVDYKNGLSRHPKASAMWFSRFLN 366
Score = 74.4 bits (156), Expect(2) = 1e-037
Identities = 26/59 (44%), Positives = 42/59 (71%)
Frame = +2 / +1
Query: 5 RKLMNYVKERYNSPTVYITENGMDDGNSPFTSIQDALKDSKRIKYHNGYLNNVAASIKE 181
RK++N+V ++Y +P +YITENGMDD + P ++ L D+KR+ + GY+ VA +IK+
Sbjct: 31 RKMINHVAKKYGNPIIYITENGMDDEDDPSATLDQVLNDTKRVGFFKGYVGAVAEAIKD 207
>gb|CN820950.1|CN820950 HRO4421_G11_M21ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4421_G11_M21, mRNA
sequence
Length = 840
Score = 67.5 bits (141), Expect(3) = 6e-022
Identities = 22/35 (62%), Positives = 32/35 (91%)
Frame = +2 / +3
Query: 179 EDGCDVRGYFAWSLLDNWEWAAGYTSRFGLYFVDY 283
++G +V GYFAWSLLDN+EW++GYTS+FG+ +VD+
Sbjct: 564 DNGANVVGYFAWSLLDNFEWSSGYTSKFGIVYVDF 668
Score = 41.8 bits (85), Expect(3) = 6e-022
Identities = 13/21 (61%), Positives = 19/21 (90%)
Frame = +2 / +3
Query: 14 MNYVKERYNSPTVYITENGMD 76
+NY+K++Y +P V+ITENGMD
Sbjct: 408 VNYIKQKYGNPAVFITENGMD 470
Score = 26.7 bits (52), Expect(3) = 6e-022
Identities = 9/17 (52%), Positives = 13/17 (76%)
Frame = +2 / +3
Query: 290 NLKRYPKNSVQWFKTLL 340
+L+R+PK S WF+ LL
Sbjct: 678 SLERHPKASAYWFRDLL 728
>gb|CN815884.1|CN815884 HRO4511_E10_J19ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4511_E10_J19, mRNA sequence
Length = 707
Score = 67.5 bits (141), Expect(3) = 6e-022
Identities = 22/35 (62%), Positives = 32/35 (91%)
Frame = +2 / +2
Query: 179 EDGCDVRGYFAWSLLDNWEWAAGYTSRFGLYFVDY 283
++G +V GYFAWSLLDN+EW++GYTS+FG+ +VD+
Sbjct: 479 DNGANVVGYFAWSLLDNFEWSSGYTSKFGIVYVDF 583
Score = 41.8 bits (85), Expect(3) = 6e-022
Identities = 13/21 (61%), Positives = 19/21 (90%)
Frame = +2 / +2
Query: 14 MNYVKERYNSPTVYITENGMD 76
+NY+K++Y +P V+ITENGMD
Sbjct: 323 VNYIKQKYGNPAVFITENGMD 385
Score = 26.7 bits (52), Expect(3) = 6e-022
Identities = 9/17 (52%), Positives = 13/17 (76%)
Frame = +2 / +2
Query: 290 NLKRYPKNSVQWFKTLL 340
+L+R+PK S WF+ LL
Sbjct: 593 SLERHPKASAYWFRDLL 643
>gb|CN821160.1|CN821160 HRO4428_B09_D18ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4428_B09_D18, mRNA
sequence
Length = 678
Score = 67.5 bits (141), Expect(3) = 6e-022
Identities = 22/35 (62%), Positives = 32/35 (91%)
Frame = +2 / +1
Query: 179 EDGCDVRGYFAWSLLDNWEWAAGYTSRFGLYFVDY 283
++G +V GYFAWSLLDN+EW++GYTS+FG+ +VD+
Sbjct: 361 DNGANVVGYFAWSLLDNFEWSSGYTSKFGIVYVDF 465
Score = 41.8 bits (85), Expect(3) = 6e-022
Identities = 13/21 (61%), Positives = 19/21 (90%)
Frame = +2 / +1
Query: 14 MNYVKERYNSPTVYITENGMD 76
+NY+K++Y +P V+ITENGMD
Sbjct: 205 VNYIKQKYGNPAVFITENGMD 267
Score = 26.7 bits (52), Expect(3) = 6e-022
Identities = 9/17 (52%), Positives = 13/17 (76%)
Frame = +2 / +1
Query: 290 NLKRYPKNSVQWFKTLL 340
+L+R+PK S WF+ LL
Sbjct: 475 SLERHPKASAYWFRDLL 525
>gb|CN821349.1|CN821349 HRO4424_C10_F20ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4424_C10_F20, mRNA
sequence
Length = 628
Score = 67.5 bits (141), Expect(3) = 6e-022
Identities = 22/35 (62%), Positives = 32/35 (91%)
Frame = +2 / +2
Query: 179 EDGCDVRGYFAWSLLDNWEWAAGYTSRFGLYFVDY 283
++G +V GYFAWSLLDN+EW++GYTS+FG+ +VD+
Sbjct: 305 DNGANVVGYFAWSLLDNFEWSSGYTSKFGIVYVDF 409
Score = 41.8 bits (85), Expect(3) = 6e-022
Identities = 13/21 (61%), Positives = 19/21 (90%)
Frame = +2 / +2
Query: 14 MNYVKERYNSPTVYITENGMD 76
+NY+K++Y +P V+ITENGMD
Sbjct: 149 VNYIKQKYGNPAVFITENGMD 211
Score = 26.7 bits (52), Expect(3) = 6e-022
Identities = 9/17 (52%), Positives = 13/17 (76%)
Frame = +2 / +2
Query: 290 NLKRYPKNSVQWFKTLL 340
+L+R+PK S WF+ LL
Sbjct: 419 SLERHPKASAYWFRDLL 469
>emb|X78433.1|ASLBDG A.sativa L. mRNA for beta-D-glucosidase
Length = 1898
Score = 81.7 bits (172), Expect(2) = 6e-021
Identities = 31/75 (41%), Positives = 45/75 (60%)
Frame = +2 / +1
Query: 107 DALKDSKRIKYHNGYLNNVAASIKEDGCDVRGYFAWSLLDNWEWAAGYTSRFGLYFVDYK 286
D L D RI+Y ++ + +I +RG+F WSL+DN+EW+ GY SRFG+ ++D
Sbjct: 1411 DPLDDPLRIEYLQQHMTAIKEAIDLGRRTLRGHFTWSLIDNFEWSLGYLSRFGIVYIDRN 1590
Query: 287 DNLKRYPKNSVQWFK 331
D KR K S +W K
Sbjct: 1591 DGCKRIMKKSAKWLK 1635
Score = 33.6 bits (67), Expect(2) = 6e-021
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = +2 / +1
Query: 5 RKLMNYVKERYNSPTVYITENGMDD 79
+ ++ +KE+Y +P +YITENG D
Sbjct: 1306 KNILLRMKEKYGNPPIYITENGTAD 1380
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,351,969
Number of Sequences: 8143
Number of extensions: 68775
Number of successful extensions: 875
Number of sequences better than 1.0e-020: 8
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 243467600
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)