TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2419355.2.1
(1174 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN819995.1|CN819995 HRO4409_F10_K19ZS5 Lib AA069E1X Aven... 86 1e-023
>gb|CN819995.1|CN819995 HRO4409_F10_K19ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4409_F10_K19, mRNA
sequence
Length = 797
Score = 86.3 bits (182), Expect(3) = 1e-023
Identities = 35/91 (38%), Positives = 59/91 (64%)
Frame = +3 / +2
Query: 333 TKTNDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEGEARQAMFEELLPATTSKLEIPY 512
+K N + +L ATN P++LD A++RRL +RI V LP+ + R+ + + LL + + +
Sbjct: 137 SKENQRILILGATNRPFDLDDAVIRRLPRRIYVGLPDAQNRKKILKILLAKENLEADFKF 316
Query: 513 DILVEKTEGYSGSDIRLVCKEAAMQPLRRLM 605
D L TEGYSGSD++ +C +A +P++ L+
Sbjct: 317 DELANATEGYSGSDLKNLCIASAYRPVQELL 409
Score = 28.6 bits (56), Expect(3) = 1e-023
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = +3 / +2
Query: 210 APSTIFLDEIDAIISQRGEA 269
AP IF+DE+D+++ RG A
Sbjct: 14 APVIIFVDEVDSLLGARGGA 73
Score = 27.6 bits (54), Expect(3) = 1e-023
Identities = 9/19 (47%), Positives = 14/19 (73%)
Frame = +3 / +2
Query: 276 EHEASRRLKTELLIQMDGL 332
EHEA+R+++ E + DGL
Sbjct: 77 EHEATRKMRNEFMAAWDGL 133
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 14,465,670
Number of Sequences: 8143
Number of extensions: 211057
Number of successful extensions: 1043
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 418381470
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)