TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2418879.2.3
(1694 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816713.1|CN816713 HRO4523_H09_P17ZS5 Lib AA071E1X Aven... 259 2e-070
gb|CN815566.1|CN815566 HRO4513_B02_C03ZS5 Lib AA071E1X Aven... 175 3e-045
gb|CN817098.1|CN817098 HRO4528_B12_D24ZS5 Lib AA071E1X Aven... 162 2e-040
gb|BE439203.1|BE439203 CDO1421_WHE03E02R ITEC CDO Oat cDNA ... 100 2e-037
gb|CN821529.1|CN821529 HRO4426_D02_G04ZS5 Lib AA069E1X Aven... 143 1e-035
>gb|CN816713.1|CN816713 HRO4523_H09_P17ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4523_H09_P17, mRNA sequence
Length = 608
Score = 259 bits (561), Expect(2) = 2e-070
Identities = 108/117 (92%), Positives = 114/117 (97%)
Frame = -2 / +2
Query: 781 KVDRSGAYVARQAAKSIVASGLARRCLVQVSYAIGVPEPLSVFVNSYGTGTIPDKEILKI 602
KVDRSGAY+ARQAAKSIVASGLARR +VQ+SYAIGVPEPLSVFV+SYGTG IPD+EILK+
Sbjct: 17 KVDRSGAYIARQAAKSIVASGLARRVIVQISYAIGVPEPLSVFVDSYGTGKIPDREILKL 196
Query: 601 VKENFDFRPGMISINLDLKKGGNRFIKTAAYGHFGRDDADFTWEVVKPLKFDKASA* 431
VKENFDFRPGMISINLDLKKGGNRFIKTAAYGHFGRDDADFTWEVVKPLKFDK SA*
Sbjct: 197 VKENFDFRPGMISINLDLKKGGNRFIKTAAYGHFGRDDADFTWEVVKPLKFDKTSA* 367
Score = 186 bits (401), Expect = 8e-048
Identities = 83/98 (84%), Positives = 87/98 (88%)
Frame = +3 / -3
Query: 423 SQP*ADALSNLRGFTTSQVKSASSRPKWP*AAVLMNLLPPFFRSRLMLIIPGLKSKFSFT 602
S *AD LSNLRGFTTSQVKSASSRPK P*AAVLMNLLPPFF+SRLMLIIPGLKSKFSFT
Sbjct: 375 SSS*ADVLSNLRGFTTSQVKSASSRPK*P*AAVLMNLLPPFFKSRLMLIIPGLKSKFSFT 196
Query: 603 ILRISLSGIVPVPYELTNTDRGSGTPMAYDTCTRQRRA 716
RISLSGI+PVP E TNTD+GSGTPMAY+ CT RRA
Sbjct: 195 SFRISLSGILPVP*ESTNTDKGSGTPMAYEICTMTRRA 82
Score = 22.6 bits (43), Expect(2) = 2e-070
Identities = 5/5 (100%), Positives = 5/5 (100%)
Frame = -2 / +1
Query: 364 CCCWM 350
CCCWM
Sbjct: 388 CCCWM 402
>gb|CN815566.1|CN815566 HRO4513_B02_C03ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4513_B02_C03, mRNA sequence
Length = 656
Score = 175 bits (377), Expect(2) = 3e-045
Identities = 71/76 (93%), Positives = 74/76 (97%)
Frame = -2 / +1
Query: 658 VFVNSYGTGTIPDKEILKIVKENFDFRPGMISINLDLKKGGNRFIKTAAYGHFGRDDADF 479
VFV+SYGTG IPD+EILK+VKENFDFRPGMISINLDLKKGGNRFIKTAAYGHFGRDDADF
Sbjct: 10 VFVDSYGTGKIPDREILKLVKENFDFRPGMISINLDLKKGGNRFIKTAAYGHFGRDDADF 189
Query: 478 TWEVVKPLKFDKASA* 431
TWEVVKPLKFDK SA*
Sbjct: 190 TWEVVKPLKFDKTSA* 237
Score = 150 bits (322), Expect = 6e-037
Identities = 69/79 (87%), Positives = 71/79 (89%)
Frame = +3 / -1
Query: 423 SQP*ADALSNLRGFTTSQVKSASSRPKWP*AAVLMNLLPPFFRSRLMLIIPGLKSKFSFT 602
S *AD LSNLRGFTTSQVKSASSRPK P*AAVLMNLLPPFF+SRLMLIIPGLKSKFSFT
Sbjct: 245 SSS*ADVLSNLRGFTTSQVKSASSRPK*P*AAVLMNLLPPFFKSRLMLIIPGLKSKFSFT 66
Query: 603 ILRISLSGIVPVPYELTNT 659
RISLSGI+PVP E TNT
Sbjct: 65 SFRISLSGILPVP*ESTNT 9
Score = 22.6 bits (43), Expect(2) = 3e-045
Identities = 5/5 (100%), Positives = 5/5 (100%)
Frame = -2 / +3
Query: 364 CCCWM 350
CCCWM
Sbjct: 258 CCCWM 272
>gb|CN817098.1|CN817098 HRO4528_B12_D24ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4528_B12_D24, mRNA sequence
Length = 718
Score = 162 bits (348), Expect = 2e-040
Identities = 63/72 (87%), Positives = 70/72 (97%)
Frame = -2 / +3
Query: 649 NSYGTGTIPDKEILKIVKENFDFRPGMISINLDLKKGGNRFIKTAAYGHFGRDDADFTWE 470
++YGTG IPD+EILK+VKENFDFRPGMISINLDLKKGGNRFIKTAAYGHFGR+D DFTWE
Sbjct: 12 DTYGTGKIPDREILKLVKENFDFRPGMISINLDLKKGGNRFIKTAAYGHFGREDPDFTWE 191
Query: 469 VVKPLKFDKASA 434
VVKPLKFDKA++
Sbjct: 192 VVKPLKFDKAAS 227
Score = 140 bits (301), Expect = 5e-034
Identities = 61/67 (91%), Positives = 62/67 (92%)
Frame = +3 / -1
Query: 441 ALSNLRGFTTSQVKSASSRPKWP*AAVLMNLLPPFFRSRLMLIIPGLKSKFSFTILRISL 620
ALSNLRGFTTSQVKS SSRPKWP*AAVLMNL PPF RSRLMLIIPGLKSKFSFT RISL
Sbjct: 220 ALSNLRGFTTSQVKSGSSRPKWP*AAVLMNLFPPFLRSRLMLIIPGLKSKFSFTSFRISL 41
Query: 621 SGIVPVP 641
SGI+PVP
Sbjct: 40 SGILPVP 20
Score = 102 bits (217), Expect = 2e-022
Identities = 40/47 (85%), Positives = 45/47 (95%)
Frame = +2 / -3
Query: 440 CLVELEGLHHLPGEVGVVTAEMAVSGGLDEPVAALLQVEVDADHPGP 580
CLV+LEGLHHLPGEVGV+TAE+AV GGLDEPV ALL+V+VDADHPGP
Sbjct: 221 CLVKLEGLHHLPGEVGVLTAEVAVGGGLDEPVPALLEVKVDADHPGP 81
Score = 93.2 bits (197), Expect(2) = 5e-028
Identities = 35/42 (83%), Positives = 39/42 (92%)
Frame = -1 / +2
Query: 644 VRHRHDPRQGDPQDREGELRLQARDDQHQPRPEEGRQQVHQD 519
+RHR DPRQGDP+ REGELRLQARDDQHQP P+EGR+QVHQD
Sbjct: 17 LRHRQDPRQGDPEAREGELRLQARDDQHQP*PQEGREQVHQD 142
Score = 47.3 bits (97), Expect(2) = 5e-028
Identities = 20/23 (86%), Positives = 20/23 (86%)
Frame = -3 / +1
Query: 510 TAISAVTTPTSPGRW*SPSSSTR 442
TA SAV TPTSPGRW*SPSS TR
Sbjct: 151 TATSAVRTPTSPGRW*SPSSLTR 219
>gb|BE439203.1|BE439203 CDO1421_WHE03E02R ITEC CDO Oat cDNA Library Avena sativa cDNA clone
CDO1421_WHE03E02, mRNA sequence
Length = 275
Score = 100 bits (214), Expect(2) = 2e-037
Identities = 40/47 (85%), Positives = 44/47 (93%)
Frame = -2 / +3
Query: 673 PEPLSVFVNSYGTGTIPDKEILKIVKENFDFRPGMISINLDLKKGGN 533
PEPLSVFV++YGTG IPDK+IL IVKENFDFRPGMI INLDLK+GGN
Sbjct: 3 PEPLSVFVDTYGTGRIPDKDILNIVKENFDFRPGMIIINLDLKRGGN 143
Score = 88.6 bits (187), Expect(2) = 3e-026
Identities = 36/46 (78%), Positives = 41/46 (89%)
Frame = +3 / -2
Query: 537 PPFFRSRLMLIIPGLKSKFSFTILRISLSGIVPVPYELTNTDRGSG 674
PP FRSRLM+I+PGLKSKFS T+LRISLSGI+PVPY TNT+ GSG
Sbjct: 139 PPLFRSRLMMIMPGLKSKFSLTMLRISLSGILPVPYVSTNTESGSG 2
Score = 71.2 bits (149), Expect(2) = 2e-037
Identities = 27/37 (72%), Positives = 31/37 (83%)
Frame = -2 / +3
Query: 541 GGNRFIKTAAYGHFGRDDADFTWEVVKPLKFDKASA* 431
G R++KTAAYGHFGRD DFTWEVVKPLK++ SA*
Sbjct: 138 GNGRYLKTAAYGHFGRDGPDFTWEVVKPLKWENPSA* 248
Score = 46.0 bits (94), Expect(2) = 3e-026
Identities = 21/30 (70%), Positives = 23/30 (76%)
Frame = +3 / -2
Query: 435 ADALSNLRGFTTSQVKSASSRPKWP*AAVL 524
A+ S+LRGFTTSQVKS S PK P AAVL
Sbjct: 244 AEGFSHLRGFTTSQVKSGPSLPKCPYAAVL 155
>gb|CN821529.1|CN821529 HRO4426_D02_G04ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4426_D02_G04, mRNA
sequence
Length = 515
Score = 143 bits (307), Expect(2) = 1e-035
Identities = 58/60 (96%), Positives = 59/60 (98%)
Frame = -2 / +1
Query: 610 LKIVKENFDFRPGMISINLDLKKGGNRFIKTAAYGHFGRDDADFTWEVVKPLKFDKASA* 431
LK+VKENFDFRPGMISINLDLKKGGNRFIKTAAYGHFGRDDADFTWEVVKPLKFDK SA*
Sbjct: 1 LKLVKENFDFRPGMISINLDLKKGGNRFIKTAAYGHFGRDDADFTWEVVKPLKFDKTSA* 180
Score = 121 bits (259), Expect = 3e-028
Identities = 55/60 (91%), Positives = 56/60 (93%)
Frame = +3 / -1
Query: 423 SQP*ADALSNLRGFTTSQVKSASSRPKWP*AAVLMNLLPPFFRSRLMLIIPGLKSKFSFT 602
S *AD LSNLRGFTTSQVKSASSRPK P*AAVLMNLLPPFF+SRLMLIIPGLKSKFSFT
Sbjct: 188 SSS*ADVLSNLRGFTTSQVKSASSRPK*P*AAVLMNLLPPFFKSRLMLIIPGLKSKFSFT 9
Score = 22.6 bits (43), Expect(2) = 1e-035
Identities = 5/5 (100%), Positives = 5/5 (100%)
Frame = -2 / +3
Query: 364 CCCWM 350
CCCWM
Sbjct: 201 CCCWM 215
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 14,233,274
Number of Sequences: 8143
Number of extensions: 198408
Number of successful extensions: 1967
Number of sequences better than 1.0e-020: 6
length of database: 1,567,487
effective HSP length: 45
effective length of database: 1,201,052
effective search space used: 623345988
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)