TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2418879.2.1
(1083 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816713.1|CN816713 HRO4523_H09_P17ZS5 Lib AA071E1X Aven... 248 9e-067
gb|CN815566.1|CN815566 HRO4513_B02_C03ZS5 Lib AA071E1X Aven... 164 2e-041
gb|CN817098.1|CN817098 HRO4528_B12_D24ZS5 Lib AA071E1X Aven... 152 8e-038
gb|BE439203.1|BE439203 CDO1421_WHE03E02R ITEC CDO Oat cDNA ... 103 6e-037
gb|CN821529.1|CN821529 HRO4426_D02_G04ZS5 Lib AA069E1X Aven... 130 5e-031
>gb|CN816713.1|CN816713 HRO4523_H09_P17ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4523_H09_P17, mRNA sequence
Length = 608
Score = 248 bits (537), Expect = 9e-067
Identities = 102/110 (92%), Positives = 108/110 (98%)
Frame = +3 / +2
Query: 327 KVDRSGAYVARQAAKSIVASGLARRCLVQVSYAIGVPEPLSVFVDSYGTGTIPDKEILKI 506
KVDRSGAY+ARQAAKSIVASGLARR +VQ+SYAIGVPEPLSVFVDSYGTG IPD+EILK+
Sbjct: 17 KVDRSGAYIARQAAKSIVASGLARRVIVQISYAIGVPEPLSVFVDSYGTGKIPDREILKL 196
Query: 507 VKENFDFRPGMITINLDLKKGGNRFIKTAAYGHFGRDDADFTWEVVKPLK 656
VKENFDFRPGMI+INLDLKKGGNRFIKTAAYGHFGRDDADFTWEVVKPLK
Sbjct: 197 VKENFDFRPGMISINLDLKKGGNRFIKTAAYGHFGRDDADFTWEVVKPLK 346
Score = 161 bits (346), Expect = 2e-040
Identities = 71/87 (81%), Positives = 77/87 (88%)
Frame = -3 / -3
Query: 652 RGLTTSQVKSASSRPKWPYAAVLMNLLPPFLRSRLMVIIPGLKSKFSLTIFRISLSGMVP 473
RG TTSQVKSASSRPK P AAVLMNLLPPF +SRLM+IIPGLKSKFS T FRISLSG++P
Sbjct: 342 RGFTTSQVKSASSRPK*P*AAVLMNLLPPFFKSRLMLIIPGLKSKFSFTSFRISLSGILP 163
Query: 472 VP*ESTNTDRGSGTPMA*DTCTRQRRA 392
VP*ESTNTD+GSGTPMA + CT RRA
Sbjct: 162 VP*ESTNTDKGSGTPMAYEICTMTRRA 82
>gb|CN815566.1|CN815566 HRO4513_B02_C03ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4513_B02_C03, mRNA sequence
Length = 656
Score = 164 bits (353), Expect = 2e-041
Identities = 65/69 (94%), Positives = 68/69 (98%)
Frame = +3 / +1
Query: 450 VFVDSYGTGTIPDKEILKIVKENFDFRPGMITINLDLKKGGNRFIKTAAYGHFGRDDADF 629
VFVDSYGTG IPD+EILK+VKENFDFRPGMI+INLDLKKGGNRFIKTAAYGHFGRDDADF
Sbjct: 10 VFVDSYGTGKIPDREILKLVKENFDFRPGMISINLDLKKGGNRFIKTAAYGHFGRDDADF 189
Query: 630 TWEVVKPLK 656
TWEVVKPLK
Sbjct: 190 TWEVVKPLK 216
Score = 130 bits (278), Expect = 5e-031
Identities = 58/68 (85%), Positives = 62/68 (91%)
Frame = -3 / -1
Query: 652 RGLTTSQVKSASSRPKWPYAAVLMNLLPPFLRSRLMVIIPGLKSKFSLTIFRISLSGMVP 473
RG TTSQVKSASSRPK P AAVLMNLLPPF +SRLM+IIPGLKSKFS T FRISLSG++P
Sbjct: 212 RGFTTSQVKSASSRPK*P*AAVLMNLLPPFFKSRLMLIIPGLKSKFSFTSFRISLSGILP 33
Query: 472 VP*ESTNT 449
VP*ESTNT
Sbjct: 32 VP*ESTNT 9
>gb|CN817098.1|CN817098 HRO4528_B12_D24ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4528_B12_D24, mRNA sequence
Length = 718
Score = 152 bits (327), Expect = 8e-038
Identities = 59/66 (89%), Positives = 64/66 (96%)
Frame = +3 / +3
Query: 459 DSYGTGTIPDKEILKIVKENFDFRPGMITINLDLKKGGNRFIKTAAYGHFGRDDADFTWE 638
D+YGTG IPD+EILK+VKENFDFRPGMI+INLDLKKGGNRFIKTAAYGHFGR+D DFTWE
Sbjct: 12 DTYGTGKIPDREILKLVKENFDFRPGMISINLDLKKGGNRFIKTAAYGHFGREDPDFTWE 191
Query: 639 VVKPLK 656
VVKPLK
Sbjct: 192 VVKPLK 209
Score = 126 bits (270), Expect = 6e-030
Identities = 55/66 (83%), Positives = 59/66 (89%)
Frame = -3 / -1
Query: 652 RGLTTSQVKSASSRPKWPYAAVLMNLLPPFLRSRLMVIIPGLKSKFSLTIFRISLSGMVP 473
RG TTSQVKS SSRPKWP AAVLMNL PPFLRSRLM+IIPGLKSKFS T FRISLSG++P
Sbjct: 205 RGFTTSQVKSGSSRPKWP*AAVLMNLFPPFLRSRLMLIIPGLKSKFSFTSFRISLSGILP 26
Query: 472 VP*EST 455
VP* S+
Sbjct: 25 VP*VSS 8
>gb|BE439203.1|BE439203 CDO1421_WHE03E02R ITEC CDO Oat cDNA Library Avena sativa cDNA clone
CDO1421_WHE03E02, mRNA sequence
Length = 275
Score = 103 bits (220), Expect(2) = 6e-037
Identities = 41/47 (87%), Positives = 44/47 (93%)
Frame = +3 / +3
Query: 435 PEPLSVFVDSYGTGTIPDKEILKIVKENFDFRPGMITINLDLKKGGN 575
PEPLSVFVD+YGTG IPDK+IL IVKENFDFRPGMI INLDLK+GGN
Sbjct: 3 PEPLSVFVDTYGTGRIPDKDILNIVKENFDFRPGMIIINLDLKRGGN 143
Score = 81.7 bits (172), Expect(2) = 4e-023
Identities = 34/46 (73%), Positives = 40/46 (86%)
Frame = -3 / -2
Query: 571 PPFLRSRLMVIIPGLKSKFSLTIFRISLSGMVPVP*ESTNTDRGSG 434
PP RSRLM+I+PGLKSKFSLT+ RISLSG++PVP STNT+ GSG
Sbjct: 139 PPLFRSRLMMIMPGLKSKFSLTMLRISLSGILPVPYVSTNTESGSG 2
Score = 66.1 bits (138), Expect(2) = 6e-037
Identities = 24/30 (80%), Positives = 26/30 (86%)
Frame = +3 / +3
Query: 567 GGNRFIKTAAYGHFGRDDADFTWEVVKPLK 656
G R++KTAAYGHFGRD DFTWEVVKPLK
Sbjct: 138 GNGRYLKTAAYGHFGRDGPDFTWEVVKPLK 227
Score = 41.4 bits (84), Expect(2) = 4e-023
Identities = 18/23 (78%), Positives = 18/23 (78%)
Frame = -3 / -2
Query: 652 RGLTTSQVKSASSRPKWPYAAVL 584
RG TTSQVKS S PK PYAAVL
Sbjct: 223 RGFTTSQVKSGPSLPKCPYAAVL 155
>gb|CN821529.1|CN821529 HRO4426_D02_G04ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4426_D02_G04, mRNA
sequence
Length = 515
Score = 130 bits (278), Expect = 5e-031
Identities = 51/53 (96%), Positives = 53/53 (100%)
Frame = +3 / +1
Query: 498 LKIVKENFDFRPGMITINLDLKKGGNRFIKTAAYGHFGRDDADFTWEVVKPLK 656
LK+VKENFDFRPGMI+INLDLKKGGNRFIKTAAYGHFGRDDADFTWEVVKPLK
Sbjct: 1 LKLVKENFDFRPGMISINLDLKKGGNRFIKTAAYGHFGRDDADFTWEVVKPLK 159
Score = 96.8 bits (205), Expect = 5e-021
Identities = 43/51 (84%), Positives = 45/51 (88%)
Frame = -3 / -1
Query: 652 RGLTTSQVKSASSRPKWPYAAVLMNLLPPFLRSRLMVIIPGLKSKFSLTIF 500
RG TTSQVKSASSRPK P AAVLMNLLPPF +SRLM+IIPGLKSKFS T F
Sbjct: 155 RGFTTSQVKSASSRPK*P*AAVLMNLLPPFFKSRLMLIIPGLKSKFSFTSF 3
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,601,506
Number of Sequences: 8143
Number of extensions: 106598
Number of successful extensions: 1329
Number of sequences better than 1.0e-020: 6
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 382105620
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)