TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2418861.2.2
(1617 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN814661.1|CN814661 HRO4501_B06_C11ZS5 Lib AA071E1X Aven... 551 e-165
>gb|CN814661.1|CN814661 HRO4501_B06_C11ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4501_B06_C11, mRNA sequence
Length = 842
Score = 551 bits (1198), Expect(2) = e-165
Identities = 216/226 (95%), Positives = 221/226 (97%)
Frame = -3 / +2
Query: 1288 ARKSNYTDMVNKYYDLATSFYEYGWGESFHFAHRWNGESLRESIKRHEHFLALQLGLKPG 1109
ARK NYTDMVNKYYDL+TSFYEYGWGESFHFAHRWNGESLRESIKRHEHFLALQL LKPG
Sbjct: 50 ARKFNYTDMVNKYYDLSTSFYEYGWGESFHFAHRWNGESLRESIKRHEHFLALQLELKPG 229
Query: 1108 MKVLDVGCGIGGPLREIARFSSTSVTGLNNNEYQITRGKELNRLAGISGTCDFVKADFMK 929
MKVLDVGCGIGGPLREIARFSSTSVTGLNNN+YQITRGKELNRLAG+SGTCDFVKADFM
Sbjct: 230 MKVLDVGCGIGGPLREIARFSSTSVTGLNNNDYQITRGKELNRLAGLSGTCDFVKADFMN 409
Query: 928 MPFDDNTFDAVYAIEATCHAPDPVGCYKEIYRVLKPGQCFAVYEWCITDHYDPNNATHKR 749
MPF DNTFDAVYAIEATCHAPDPVGCYKEIYRVLKPGQCFAVYEWCITDHYDPNNATHKR
Sbjct: 410 MPFSDNTFDAVYAIEATCHAPDPVGCYKEIYRVLKPGQCFAVYEWCITDHYDPNNATHKR 589
Query: 748 IKDEIELGNGLPDIRSTRQCLQAVKDAGFEVVWDKDLAEDSPLPWY 611
IKDEIELGNGLPDIRST+QCLQAVKDAGFEV+WDKDLA DSPLPWY
Sbjct: 590 IKDEIELGNGLPDIRSTQQCLQAVKDAGFEVIWDKDLAVDSPLPWY 727
Score = 400 bits (867), Expect(2) = e-123
Identities = 172/241 (71%), Positives = 197/241 (81%)
Frame = +2 / -2
Query: 599 IQGQVPRQGRIFSKILIPNNLKPGVFYCLETLPSASDIWQAIAKLNFILDPFVGCIVRII 778
IQGQVPRQ RI+SKILIPNNLKP +F L+TL SASDIW I KLNFIL+P VGCIV II
Sbjct: 739 IQGQVPRQWRIYSKILIPNNLKPCIFNSLKTLLSASDIW*TITKLNFILNPLVGCIVWII 560
Query: 779 VIRNAPLVHGKALARLQHTIYLLVATNWIRCMTCCLNGVNSIKSVVIERHLHEVRLDKIT 958
VI NAPL++ K L R +T+ LL+A N IRCMTCCLNG+NSIKSV+ ERH+HEV LDK+T
Sbjct: 559 VISNAPLIYSKTLPRF*YTVDLLIAANRIRCMTCCLNGINSIKSVIGERHIHEVCLDKVT 380
Query: 959 CSTNSC*TVELLSPGYLVFVVIQSGN*S*AKSCNFSQWSTYSTAHI*NLHSWFQTKLQGK 1138
ST SC T+ELLSP YL+ VV+QSGN* *AKS N S WSTYSTAH+ NLH WFQ KL+G+
Sbjct: 379 SSTKSCQTIELLSPSYLIVVVVQSGN*G*AKSGNLS*WSTYSTAHVQNLHPWFQLKLKGQ 200
Query: 1139 KMLMSLDAFT*GFSIPSVSKVEGFTPTILIEASGKIIVFINHISIVGLPCFLLPSIVSMI 1318
KML+S DAF * F IP VSKV+ FTP +LIEAS KI+VFINHIS+V LPCFLLPS+VS
Sbjct: 199 KMLVSFDAFP*RFPIPPVSKVKRFTPAVLIEASRKIVVFINHISVVELPCFLLPSVVSRN 20
Query: 1319 F 1321
F
Sbjct: 19 F 17
Score = 364 bits (790), Expect = e-101
Identities = 167/239 (69%), Positives = 179/239 (74%)
Frame = +1 / -1
Query: 595 LDPRASTKARENLQQDPYPKQPQTRRLLLPGDIAECF*YLAGHCQAQFHP*SFCGLHC*D 774
LDPRASTKA ENLQQDPYPK PQT L DIAECF*YL H QAQFHP*S CGLHC D
Sbjct: 743 LDPRASTKAMENLQQDPYPK*PQTLHL*QLEDIAECF*YLVDHYQAQFHP*SSCGLHCLD 564
Query: 775 HSDP*CTTRTRQSTGQASTHDISPCSNQLDQVHDMLPQWRKQHQKCCHRTASS*SPP*QN 954
HSD *CTT +Q+T Q H SP S+Q DQVHDMLPQW KQHQKC R A S*S P*Q+
Sbjct: 563 HSDQ*CTTHIQQNTAQVLIHGRSPYSSQPDQVHDMLPQWHKQHQKCYRRKAYS*SLP*QS 384
Query: 955 HMFH*FLLNG*APFPWLSGIRCYSIR*LKLS*ILQFLSVVHLFHSPHLKPSFLVSNQVAG 1134
H FH* L + *APFP LS RC SIR*L+LS*I Q L +VHLFHSP KPS LVS Q G
Sbjct: 383 HKFH*VLPDD*APFP*LSDSRCCSIR*LRLS*IWQSLLMVHLFHSPRPKPSSLVSTQAEG 204
Query: 1135 QENAHVA*CFHVRILHSICEQSGRIHPNHTHRS*WQDHSIY*PYQYSWTSLLPPSLHSI 1311
ENA V *CF ++I HS CEQS +IHP+ THRS* +D SIY*PYQ S TSLLPPSL SI
Sbjct: 203 PENARVV*CFPLKIPHSTCEQSEKIHPSRTHRS**KDRSIY*PYQCS*TSLLPPSLRSI 27
Score = 331 bits (718), Expect(3) = e-111
Identities = 154/171 (90%), Positives = 157/171 (91%)
Frame = +3 / -3
Query: 777 **SVMHHSYTAKHWPGFNTRYISL*QPTGSGA*HVASMA*TASKVLSSNGIFMKSALTKS 956
**SVMHHSYTAKH PGFNTR ISL*QPTGSGA*HVASMA*TASKVLS GIFMKSALTKS
Sbjct: 561 **SVMHHSYTAKHCPGFNTR*ISL*QPTGSGA*HVASMA*TASKVLSEKGIFMKSALTKS 382
Query: 957 HVPLIPAKRLSSFPLVIWYSLLFNPVTEVELNLAISLSGPPIPQPTSKTFIPGFKPSCRA 1136
VPL PA+RLSSFPLVI SLLFNPVTEVELNLAISL+GPPIPQPTSKTFIPGF S RA
Sbjct: 381 QVPLSPARRLSSFPLVI**SLLFNPVTEVELNLAISLNGPPIPQPTSKTFIPGFNSS*RA 202
Query: 1137 RKCSCRLMLSRKDSPFHL*AKWKDSPQPYS*KLVARS*YLLTISV*LDFLA 1289
RKCSCRLMLS KDSPFHL*AK KDSPQPYS*KLV RS*YLLTISV*L+FLA
Sbjct: 201 RKCSCRLMLSLKDSPFHL*AK*KDSPQPYS*KLVERS*YLLTISV*LNFLA 49
Score = 305 bits (661), Expect(3) = 6e-098
Identities = 136/200 (68%), Positives = 150/200 (75%)
Frame = -2 / +1
Query: 1196 CSQMEWRILT*KHQAT*AFSCPATWFETRNEGFRCGLWNRWTTERNCKI*LNFSYRIE*Q 1017
CSQ+EW I KHQ T AFS P+ ETR+EGF GLWNRWT +R+C+I*LN SYRIE Q
Sbjct: 142 CSQVEWGIFKGKHQTTRAFSGPSA*VETRDEGFGRGLWNRWTIKRDCQI*LNLSYRIEQQ 321
Query: 1016 RIPDNQGKGAQPFSRN*WNM*FCQGGLHEDAVR*QHF*CCLRH*GNMSCT*SSWLLQGDI 837
R+ DN GKGAQ R *WN+* CQG LHE A HF*CCL H*GNMSCT*S WLL GD+
Sbjct: 322 RLSDN*GKGAQSSGRT*WNL*LCQGRLHEYAFLR*HF*CCLCH*GNMSCT*SGWLL*GDL 501
Query: 836 SCVEAWPVLCRVRVVHYGSL*S*QCNPQKDQG*N*AWQWPARYQKHSAMSPGSKRRRV*G 657
C++ W V C + VVHY SL*S QCNPQ+D G*N*AW W RYQKHSAMS R RV*G
Sbjct: 502 PCIKTWAVFCCI*VVHY*SL*SKQCNPQED*G*N*AW*WSTRYQKHSAMSSSC*RCRV*G 681
Query: 656 CLG*GSC*RFSLALVLALGS 597
LG*GSC RFS+ALVLALGS
Sbjct: 682 YLG*GSCCRFSIALVLALGS 741
Score = 155 bits (332), Expect(3) = 2e-082
Identities = 73/104 (70%), Positives = 79/104 (75%)
Frame = -1 / +3
Query: 903 MLFTPLRQHVMHLIQLVATRRYIVC*SLASALPCTSGALRITMILTMQPTKGSRMKLSLA 724
MLF PLRQHVMHLI+L A RR V *+L S L SGAL ITMI TMQPT+G RMKLSL
Sbjct: 435 MLFMPLRQHVMHLIRLAAIRRSTVY*NLGSVLLYMSGALLITMIQTMQPTRGLRMKLSLV 614
Query: 723 MACQISEALGNVSRQ*KTPGLRLFGIRILLKILPCLGTCPWIQA 592
M QISEAL NV + K GLRLFGIRILL IL CLGTCPWI++
Sbjct: 615 MVYQISEALSNVFKLLKMQGLRLFGIRILL*ILHCLGTCPWIKS 746
Score = 146 bits (313), Expect(3) = 2e-082
Identities = 78/120 (65%), Positives = 83/120 (69%)
Frame = -1 / +3
Query: 1314 MDTMEGRRKQGSPTILIWLINTMILPLASMSMVGVNPSTLLTDGMENPYVKASSDMSIFL 1135
+DT EGRRKQGS T LIWLINT I LASMS GVN TLLT GM N KAS+D SIF
Sbjct: 24 LDTTEGRRKQGSSTTLIWLINTTIFLLASMSTAGVNLFTLLTGGMGNL*GKASNDTSIFW 203
Query: 1134 PCNLV*NQE*RF*MWAVE*VDH*EKXXXXXXXXLPD*ITTNTR*PGERSSTV*QELVEHV 955
P +L *NQ *RF WAVE*VDH*E+ LPD* TT R* GERSS V Q+LVE V
Sbjct: 204 PFSLS*NQG*RFWTWAVE*VDH*ERLPDLAQPQLPD*TTTTIR*LGERSSIVWQDLVELV 383
Score = 65.2 bits (136), Expect(3) = e-111
Identities = 27/32 (84%), Positives = 28/32 (87%)
Frame = +3 / -3
Query: 600 SKGKYQGKGESSARSLSQTTSNPASFTAWRHC 695
SKGKYQG GES+ARSLSQ TSNPAS TA RHC
Sbjct: 738 SKGKYQGNGESTARSLSQITSNPASLTA*RHC 643
Score = 57.4 bits (119), Expect(2) = e-123
Identities = 25/32 (78%), Positives = 26/32 (81%)
Frame = +3 / -1
Query: 501 GARPTYSRALTIVRVIMRPTEVKRKLLRENRL 596
GARP YS ALTI+RV RPT VKRKLL ENRL
Sbjct: 836 GARPMYSNALTILRVTSRPTVVKRKLLNENRL 741
Score = 46.9 bits (96), Expect(2) = e-165
Identities = 17/23 (73%), Positives = 22/23 (95%)
Frame = -3 / +1
Query: 565 TSVGRMITRTMVKALEYVGLAPQ 497
T+VGR++TR MVKALEY+GLAP+
Sbjct: 772 TTVGRLVTRNMVKALEYIGLAPE 840
Score = 45.1 bits (92), Expect(3) = 6e-098
Identities = 22/27 (81%), Positives = 24/27 (88%)
Frame = -2 / +1
Query: 1313 WILWREGGSKEVQLY*YG**IL*SCH* 1233
WIL REGGSKEVQL+*YG**IL S +*
Sbjct: 25 WILRREGGSKEVQLH*YG**ILRSFY* 105
Score = 42.8 bits (87), Expect(3) = 6e-098
Identities = 18/32 (56%), Positives = 19/32 (59%)
Frame = -2 / +3
Query: 596 KPILPE*LPFDLCGTHDYPHNGQGPGVRWSCS 501
K I E* PFD GT Y GQG G+ WSCS
Sbjct: 741 KSIFIE*FPFDYRGTTCYSQYGQGIGIHWSCS 836
Score = 41.4 bits (84), Expect(3) = e-111
Identities = 19/32 (59%), Positives = 22/32 (68%)
Frame = +1 / -2
Query: 502 EQDQRTPGP*PLCG*SCVPQRSNGSYSGRIGL 597
EQDQ P P*P C * VP+ SNG+YS +I L
Sbjct: 835 EQDQCIPMP*PYCE*QVVPR*SNGNYSMKIDL 740
Score = 40.0 bits (81), Expect(3) = 2e-082
Identities = 21/35 (60%), Positives = 22/35 (62%)
Frame = -1 / +2
Query: 606 PWIQADSP*VASV*PLWDA*LPAQWSRPWSTLVLL 502
P Q D *V SV* WD L A WSR W+TLVLL
Sbjct: 731 PLDQVDFH*VVSV*LPWDDLLLAIWSRHWNTLVLL 835
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 18,699,333
Number of Sequences: 8143
Number of extensions: 291983
Number of successful extensions: 1292
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 45
effective length of database: 1,201,052
effective search space used: 592118636
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)