TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2404863.2.1
(672 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816486.1|CN816486 HRO4521_C09_E17ZS5 Lib AA071E1X Aven... 201 1e-052
gb|CN817629.1|CN817629 HRO4463_B12_C23ZS5 Lib AA070E1X Aven... 201 1e-052
>gb|CN816486.1|CN816486 HRO4521_C09_E17ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4521_C09_E17, mRNA sequence
Length = 431
Score = 201 bits (433), Expect = 1e-052
Identities = 82/87 (94%), Positives = 84/87 (96%)
Frame = +1 / +2
Query: 1 GPGCVVEDGVRLSRCTVMRGVRIKKHACISNSIIGWHSTVGQWARIENMTILGEDVHGGD 180
GPGCVVEDGVRLSRCTVMRG RIKKHACISNSI+GWHSTVG+WARIENMTILGEDVH GD
Sbjct: 41 GPGCVVEDGVRLSRCTVMRGARIKKHACISNSIVGWHSTVGKWARIENMTILGEDVHVGD 220
Query: 181 EVYSNGGVVLPQKEIKSSILKPEIVM* 261
EVYSNGGVVLP KEIKSSILKPEIVM*
Sbjct: 221 EVYSNGGVVLPHKEIKSSILKPEIVM* 301
Score = 155 bits (334), Expect = 5e-039
Identities = 59/86 (68%), Positives = 69/86 (80%)
Frame = +3 / +1
Query: 3 TWMCRGGWREAFPLHCHAWCAYQEACLHLEQHYRVALNCWTMGTDREYDHPGRGCSWG*R 182
TWMC GGW EAFP++ AW AY EACLHL+QH +AL+CW MGT R+YD+PGRGC+ *
Sbjct: 43 TWMCCGGWCEAFPMYGDAWRAY*EACLHLKQHCWMALDCWKMGTHRKYDYPGRGCTCR** 222
Query: 183 GIQQWRCCSPTERDQVKHFEA*DRHV 260
G+QQW CCS T+RDQVKH EA DRHV
Sbjct: 223 GLQQWWCCSTTQRDQVKHPEARDRHV 300
Score = 137 bits (294), Expect = 2e-033
Identities = 59/87 (67%), Positives = 67/87 (77%)
Frame = -1 / -2
Query: 261 SHDDLRLQNA*LDLFLWENNTAIAVYLVTPMNILAQDGHILYPCPLSNS*VPPDNAVRDA 82
SHDDL LQ+A*LDLF+W NNT IAV L+T M IL+QD HI Y CP SNS VP +NAV DA
Sbjct: 301 SHDDLGLQDA*LDLFVW*NNTTIAVDLITYMYILSQDSHIFYACPFSNSRVPSNNAV*DA 122
Query: 81 GMLLDTHTTHDSAAGKPHAILHDTSRS 1
MLL+T TH +GKPH ILH+TSRS
Sbjct: 121 SMLLNTRATHHRTSGKPHTILHNTSRS 41
Score = 122 bits (261), Expect = 6e-029
Identities = 59/87 (67%), Positives = 64/87 (73%)
Frame = +2 / +3
Query: 2 DLDVSWRMA*GFPAALSCVVCVSRSMPASRTALSGGTQLLDNGHG*RI*PSWARMFMGVT 181
DLDV WRM *GFP CV V RSM AS+TAL GT+LL+NGH *+I* SW RM+M V
Sbjct: 42 DLDVLWRMV*GFPDVR*CVARVLRSMLASQTALLDGTRLLENGHA*KI*LSWERMYM*VM 221
Query: 182 RYTAMAVLFSHRKRSSQAF*SLRSSCE 262
R TAM VLF H KRSSQA *S RSSCE
Sbjct: 222 RSTAMVVLFYHTKRSSQAS*SPRSSCE 302
Score = 111 bits (237), Expect(2) = 1e-032
Identities = 44/51 (86%), Positives = 47/51 (92%)
Frame = -2 / -3
Query: 155 RMVIFSIRAHCPTVECHPIMLFEMQACFLIRTPRMTVQRESLTPSSTTHPG 3
R+VIFS+RAH PTVECHP MLFEMQACFLIR PR+TV RESLTPSSTTHPG
Sbjct: 195 RIVIFSMRAHFPTVECHPTMLFEMQACFLIRAPRITVHRESLTPSSTTHPG 43
Score = 43.2 bits (88), Expect(2) = 1e-032
Identities = 16/19 (84%), Positives = 18/19 (94%)
Frame = -2 / -3
Query: 263 SHMTISGFKMLDLISFCGR 207
+HMTISGF+MLDLIS CGR
Sbjct: 303 AHMTISGFRMLDLISLCGR 247
>gb|CN817629.1|CN817629 HRO4463_B12_C23ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4463_B12_C23, mRNA sequence
Length = 781
Score = 201 bits (433), Expect = 1e-052
Identities = 82/87 (94%), Positives = 84/87 (96%)
Frame = +1 / +2
Query: 1 GPGCVVEDGVRLSRCTVMRGVRIKKHACISNSIIGWHSTVGQWARIENMTILGEDVHGGD 180
GPGCVVEDGVRLSRCTVMRG RIKKHACISNSI+GWHSTVG+WARIENMTILGEDVH GD
Sbjct: 341 GPGCVVEDGVRLSRCTVMRGARIKKHACISNSIVGWHSTVGKWARIENMTILGEDVHVGD 520
Query: 181 EVYSNGGVVLPQKEIKSSILKPEIVM* 261
EVYSNGGVVLP KEIKSSILKPEIVM*
Sbjct: 521 EVYSNGGVVLPHKEIKSSILKPEIVM* 601
Score = 160 bits (345), Expect = 2e-040
Identities = 61/86 (70%), Positives = 71/86 (82%)
Frame = +3 / +1
Query: 3 TWMCRGGWREAFPLHCHAWCAYQEACLHLEQHYRVALNCWTMGTDREYDHPGRGCSWG*R 182
TWMC GGWREAFP++ AWCAY EACLHL+QH +AL+C MGT R+YD+PGRGC+ *
Sbjct: 343 TWMCCGGWREAFPMYGDAWCAY*EACLHLKQHCWMALDCRKMGTHRKYDYPGRGCTCR** 522
Query: 183 GIQQWRCCSPTERDQVKHFEA*DRHV 260
G+QQW CCS T+RDQVKH EA*DRHV
Sbjct: 523 GLQQWWCCSTTQRDQVKHPEA*DRHV 600
Score = 142 bits (305), Expect = 5e-035
Identities = 61/87 (70%), Positives = 70/87 (80%)
Frame = -1 / -1
Query: 261 SHDDLRLQNA*LDLFLWENNTAIAVYLVTPMNILAQDGHILYPCPLSNS*VPPDNAVRDA 82
SHDDLRLQ+A*LDLF+W NNT IAV L+T M IL+QD HI Y CP S+S VP +NAV DA
Sbjct: 601 SHDDLRLQDA*LDLFVW*NNTTIAVDLITYMYILSQDSHIFYACPFSDSRVPSNNAV*DA 422
Query: 81 GMLLDTHTTHDSAAGKPHAILHDTSRS 1
MLL+T TTH +GKPHAILH+TSRS
Sbjct: 421 SMLLNTRTTHHRTSGKPHAILHNTSRS 341
Score = 126 bits (270), Expect = 3e-030
Identities = 61/87 (70%), Positives = 66/87 (75%)
Frame = +2 / +3
Query: 2 DLDVSWRMA*GFPAALSCVVCVSRSMPASRTALSGGTQLLDNGHG*RI*PSWARMFMGVT 181
DLDV WRMA*GFP CVV V RSM AS+TAL GT+L +NGH *+I* SW RM+M V
Sbjct: 342 DLDVLWRMA*GFPDVR*CVVRVLRSMLASQTALLDGTRLSENGHA*KI*LSWERMYM*VM 521
Query: 182 RYTAMAVLFSHRKRSSQAF*SLRSSCE 262
R TAM VLF H KRSSQA *SLRSSCE
Sbjct: 522 RSTAMVVLFYHTKRSSQAS*SLRSSCE 602
Score = 111 bits (237), Expect(2) = 1e-032
Identities = 44/51 (86%), Positives = 47/51 (92%)
Frame = -2 / -2
Query: 155 RMVIFSIRAHCPTVECHPIMLFEMQACFLIRTPRMTVQRESLTPSSTTHPG 3
R+VIFS+RAH PTVECHP MLFEMQACFLIR PR+TV RESLTPSSTTHPG
Sbjct: 495 RIVIFSMRAHFPTVECHPTMLFEMQACFLIRAPRITVHRESLTPSSTTHPG 343
Score = 80.8 bits (170), Expect(2) = 2e-022
Identities = 35/49 (71%), Positives = 36/49 (73%)
Frame = -3 / -3
Query: 262 LT*RSQASKCLT*SLSVGEQHRHCCIPRHPHEHPRPGWSYSLSVPIVQQ 116
LT*RSQAS CLT*SL V EQH HCC P H H HP PG SY L VPI +Q
Sbjct: 602 LT*RSQASGCLT*SLCVVEQHHHCCRPHHLHVHPLPG*SYFLCVPIFRQ 456
Score = 43.2 bits (88), Expect(2) = 1e-032
Identities = 16/19 (84%), Positives = 18/19 (94%)
Frame = -2 / -2
Query: 263 SHMTISGFKMLDLISFCGR 207
+HMTISGF+MLDLIS CGR
Sbjct: 603 AHMTISGFRMLDLISLCGR 547
Score = 39.1 bits (79), Expect(2) = 2e-022
Identities = 15/20 (75%), Positives = 16/20 (80%)
Frame = -3 / -3
Query: 61 HHA*QCSGKASRHPPRHIQV 2
HHA GKASRHPP+HIQV
Sbjct: 401 HHASPYIGKASRHPPQHIQV 342
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,500,866
Number of Sequences: 8143
Number of extensions: 118060
Number of successful extensions: 1380
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 221812061
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)