TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 2404820.2.1
         (830 letters)

Database: Avena_nucl_with_EST.fasta 
           8143 sequences; 4,702,463 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CN818432.1|CN818432  HRO4460_F09_K18ZS5 Lib AA070E1X Aven...   189   2e-076
gb|CN821039.1|CN821039  HRO4422_H02_O04ZS5 Lib AA069E1X Aven...   122   8e-029

>gb|CN818432.1|CN818432 HRO4460_F09_K18ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
           Avena sativa cDNA clone HRO4460_F09_K18, mRNA sequence
          Length = 869

 Score =  189 bits (407), Expect(3) = 2e-076
 Identities = 76/101 (75%), Positives = 87/101 (86%)
 Frame = -2 / +3

                                                                       
Query: 430 HMTVQDIFVCILAFMPTGWGLLLIAQTMRSAISHMGLWGSVKALARGYEIIMGLLLFTPI 251
           HMT++DIFVCILAF+PTGWG+LLIAQ  R  +   GLWGSV+ALAR YE+IMG+LLFTPI
Sbjct: 387 HMTIRDIFVCILAFLPTGWGILLIAQACRPVLRVAGLWGSVRALARAYEVIMGVLLFTPI 566

                                                    
Query: 250 AFLAWFPFVSEFQPRMLFNQAFSRGLQISRILGGHKKDRAA 128
             L+WFPFVSEFQ RMLFNQAFSRGLQISRILGG K+  A+
Sbjct: 567 TVLSWFPFVSEFQTRMLFNQAFSRGLQISRILGGQKERAAS 689




 Score =  153 bits (328), Expect(2) = 6e-051
 Identities = 78/158 (49%), Positives = 89/158 (56%)
 Frame = +3 / -2

                                                                       
Query: 150 PPRIREICRPLLKAWLNSILGWNSETNGNQARNAIGVNNSKPMMIS*PRASALTDPHSPI 329
           PPRIREI  PLL A LNSI  WNSET GN  R  +GV +S PM+ S  RA A TDPHSP 
Sbjct: 667 PPRIREI*SPLLNA*LNSIRVWNSETKGNHDRTVMGVKSSTPMITSYARARARTDPHSPA 488

                                                                       
Query: 330 CEIADLIVCAIRSKPHPVGMNARMQTKMS*TVMCGIAIKIMIIEINVMNISPLISRNTSW 509
                L  CAI S PHPVG NARMQT +S  VM  ++I       N  NI   ISR  S 
Sbjct: 487 TRRTGLQACAISSIPHPVGKNARMQTNISRIVM*RMSINTTSTAANATNIMNFISRKNS* 308

                                                 
Query: 510 NSALNFLLPTDTVFITSKMTKIMTHEMX*TRTLFV*VV 623
             A N   PTD  FI +   +I +HE   T+ L   V+
Sbjct: 307 KLAPNLRRPTDITFIINNAPRITSHENKYTKILLSSVI 194




 Score =  103 bits (220), Expect = 3e-023
 Identities = 54/156 (34%), Positives = 89/156 (57%)
 Frame = +2 / -1

                                                                       
Query: 116 LLVPCSPVFLVSA*DTRNLQTSTEGLVEQHPWLELRNKREPSEECDRGEQQQAHDDLIAS 295
           L+   S   L++  D  +L++S E L+EQH  LEL ++ +P +  D  ++Q  HD L+  
Sbjct: 701 LVFGASGPLLLATKDPGDLKSSAERLIEQHSRLELGDEGKP*QNRDGCKEQHPHDYLVRP 522

                                                                       
Query: 296 GKRLDRSP*PHM*NS*PHSLCYQKQTPSSWHERKDANKDVLNCHVWDCYQDYDNRDKRYE 475
            +   RSP*P    + P SLC Q+ +PS   ER+DA+K +   HV + YQ   +  +  +
Sbjct: 521 CQGTHRSP*PCYPKNRPASLCDQQYSPSGGQERQDADKYISYSHVKNEYQHNQHSSECNK 342

                                               
Query: 476 YQPLDQPEHKLELCTEFPPAN*YSLHHEQNDKDYDP 583
           Y  L Q E  L+  T+ P ++ Y+LH++Q  ++Y+P
Sbjct: 341 YHELYQSEE*LKTRTKSPASDRYNLHNKQRPQNYEP 234




 Score = 92.3 bits (195), Expect(3) = 2e-076
 Identities = 35/47 (74%), Positives = 39/47 (82%)
 Frame = -2 / +3

                                                          
Query: 760 EKXXXSWWEKEQEXLRHSGKRGTVLEIVLSLRFFIYQYGLVYHLNIT 620
           EK   SWWEKEQE L+HSG  G + EI+LSLRFF+YQYGLVYHLNIT
Sbjct: 60  EKSWESWWEKEQEHLKHSGALGNLFEIILSLRFFLYQYGLVYHLNIT 200




 Score = 62.9 bits (131), Expect(2) = 6e-051
 Identities = 28/47 (59%), Positives = 31/47 (65%)
 Frame = +3 / -2

                                                          
Query: 621 VMFK**TRPYW*IKKRNESTISRTVPRFPECLRXSCSFSHHDXXLFS 761
           V+FK *TRPY * K R++  IS   P  PEC R SCSFSH D  LFS
Sbjct: 199 VIFKW*TRPY**RKNRSDKIISNKFPSAPECFRCSCSFSHQDSQLFS 59




 Score = 38.6 bits (78), Expect(3) = 2e-076
 Identities = 15/21 (71%), Positives = 17/21 (80%)
 Frame = -2 / +3

                                
Query: 553 MKTVSVGRRKFSAEFQLVFRL 491
           MK +SVGRR+F A FQL FRL
Sbjct: 264 MKVISVGRRRFGASFQLFFRL 326



>gb|CN821039.1|CN821039 HRO4422_H02_O04ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
           leaf Avena sativa cDNA clone HRO4422_H02_O04, mRNA
           sequence
          Length = 700

 Score =  122 bits (261), Expect = 8e-029
 Identities = 47/96 (48%), Positives = 64/96 (66%)
 Frame = -2 / +2

                                                                       
Query: 427 MTVQDIFVCILAFMPTGWGLLLIAQTMRSAISHMGLWGSVKALARGYEIIMGLLLFTPIA 248
           +++ D+ +C LAF+PTGWGLLLI Q +R  I +  +W  ++ +A  Y+  MG LLF PIA
Sbjct: 89  LSIMDLIICCLAFIPTGWGLLLIVQVLRPKIEYYAIWEPIQVIAHAYDYGMGSLLFFPIA 268

                                               
Query: 247 FLAWFPFVSEFQPRMLFNQAFSRGLQISRILGGHKK 140
            LAW P +S  Q R+LFN+AFSR LQI   + G  K
Sbjct: 269 ILAWMPIISAIQTRVLFNRAFSRQLQIQPFIVGKTK 376



  Database: Avena_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:15 PM
  Number of letters in database: 4,702,463
  Number of sequences in database:  8143
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 8,848,510
Number of Sequences: 8143
Number of extensions: 118137
Number of successful extensions: 903
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 283639754
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)