TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2306429.2.1
(1175 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN819880.1|CN819880 HRO4407_C04_F07ZS5 Lib AA069E1X Aven... 61 5e-030
>gb|CN819880.1|CN819880 HRO4407_C04_F07ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4407_C04_F07, mRNA
sequence
Length = 741
Score = 60.6 bits (126), Expect(4) = 5e-030
Identities = 26/57 (45%), Positives = 37/57 (64%)
Frame = +3 / +1
Query: 309 YNLGTGRGTSVLEIVKAFEKASGKKIPLIFGERRPGDAEILFSETTKAERELNWKAK 479
YN+GTG+G SV E V+A + A+G I + + RRPGD ++S +K ELNW A+
Sbjct: 400 YNVGTGQGRSVKEFVEACKAATGATIKVEYLTRRPGDYAEVYSNPSKIRDELNWTAQ 570
Score = 56.5 bits (117), Expect(4) = 5e-030
Identities = 24/42 (57%), Positives = 26/42 (61%)
Frame = +3 / +1
Query: 150 AVGRRPALTVLGNDYATRDGTGVRDYIHVVDLADGHIAALQK 275
A G P L V G DY T DGT +RDYI V DL D H+ AL K
Sbjct: 250 ASGTIPGLKVRGTDYPTADGTCIRDYIDVTDLVDAHVKALGK 375
Score = 38.6 bits (78), Expect(4) = 5e-030
Identities = 13/23 (56%), Positives = 18/23 (78%)
Frame = +3 / +1
Query: 42 IILLRYFNPVGAHPSGYLGEDPR 110
+++LRYFN +G+ P G LGE PR
Sbjct: 133 VMILRYFNVIGSDPEGLLGEAPR 201
Score = 25.8 bits (50), Expect(4) = 5e-030
Identities = 8/21 (38%), Positives = 10/21 (47%)
Frame = +3 / +1
Query: 486 IEEMCRDQWNWASKNPYGYGS 548
+ E W W +P GYGS
Sbjct: 580 LRESLATAWKWQKTHPGGYGS 642
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 14,192,663
Number of Sequences: 8143
Number of extensions: 218246
Number of successful extensions: 1052
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 419590665
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)