TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2276392.2.1
(644 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815407.1|CN815407 HRO4509_C10_E19ZS5 Lib AA071E1X Aven... 226 3e-060
gb|CN817735.1|CN817735 HRO4461_E02_J03ZS5 Lib AA070E1X Aven... 223 3e-059
gb|CN816832.1|CN816832 HRO4525_C10_E19ZS5 Lib AA071E1X Aven... 216 2e-057
gb|CN821505.1|CN821505 HRO4426_B01_C02ZS5 Lib AA069E1X Aven... 157 2e-039
gb|CN820354.1|CN820354 HRO4418_D08_G16ZS5 Lib AA069E1X Aven... 150 2e-037
gb|CN815372.1|CN815372 HRO4508_H11_P22ZS5 Lib AA071E1X Aven... 136 3e-033
gb|CN814739.1|CN814739 HRO4502_A01_A02ZS5 Lib AA071E1X Aven... 124 7e-032
gb|CN820073.1|CN820073 HRO4410_G08_M16ZS5 Lib AA069E1X Aven... 108 1e-024
gb|CN820934.1|CN820934 HRO4421_F07_K13ZS5 Lib AA069E1X Aven... 96 6e-021
>gb|CN815407.1|CN815407 HRO4509_C10_E19ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4509_C10_E19, mRNA sequence
Length = 573
Score = 226 bits (488), Expect = 3e-060
Identities = 93/107 (86%), Positives = 94/107 (87%)
Frame = -3 / -3
Query: 402 SLVFWIFNTFFTIFCSSTKKARMILSLTAVPDRTPPYGRFTVLWFLANLDLLYSAGLRCG 223
SLVFWIF TFFTIFCSST+ ARMILSLTA PDRTPP GRFTVL FL NLDLLYS GLR G
Sbjct: 328 SLVFWIFRTFFTIFCSSTRNARMILSLTAGPDRTPP*GRFTVLLFLDNLDLLYSVGLR*G 149
Query: 222 IPWIFFPVTGHFGPLARFLVYWYTSFPPGVLTTRVWFDLVA*LCRFR 82
IPWIF PVTGHFGPLA F VYWYTSFPPGVLTT VW DLVA*LCRFR
Sbjct: 148 IPWIFLPVTGHFGPLALFFVYWYTSFPPGVLTTLVWLDLVA*LCRFR 8
Score = 210 bits (453), Expect = 2e-055
Identities = 86/90 (95%), Positives = 88/90 (97%)
Frame = +2 / +3
Query: 77 TYRKRHSYATKSNQTRVVKTPGGKLVYQYTKKRASGPKCPVTGKKIQGIPHLRPAEYKRS 256
TYRKRHSYATKSNQTRVVKTPGGKLVYQYTKKRASGPKCPVTGKKIQGIPHLRP EYKRS
Sbjct: 3 TYRKRHSYATKSNQTRVVKTPGGKLVYQYTKKRASGPKCPVTGKKIQGIPHLRPTEYKRS 182
Query: 257 RLARNQRTVNRPYGGVLSGTAVRERIIRAF 346
RL+RN+RTVNRPYGGVLSG AVRERIIRAF
Sbjct: 183 RLSRNRRTVNRPYGGVLSGPAVRERIIRAF 272
Score = 144 bits (309), Expect = 1e-035
Identities = 60/108 (55%), Positives = 78/108 (72%)
Frame = +1 / +2
Query: 79 LPEAAQLRHQIEPDPRRQDTWWETCVPVHQEARERTEVPSHREEDPRNSTPETC*VQEVQ 258
+PEAAQLRHQ++PD RQD E VPVH+E ER E+P HR+EDP +S+PE *VQ+VQ
Sbjct: 5 VPEAAQLRHQVQPDQGRQDPRREARVPVHEEEGERAEMPGHRQEDPGDSSPEAY*VQKVQ 184
Query: 259 VGKEPKDCESSIWRCSVWYCS*GEDHPRLFG*GTEDSEEGVEDPKDQG 402
+ +E ++ E S+W CS+W *GEDHP + +D EEG EDP+DQG
Sbjct: 185 IIQEQENREPSLWWCSIWPGC*GEDHPSIPCGRAKDREEGSEDPEDQG 328
Score = 116 bits (247), Expect = 5e-027
Identities = 55/87 (63%), Positives = 59/87 (67%)
Frame = -2 / -2
Query: 340 ADDPLPNCSTRQNTAIWTIHSPLVPCQPGPLVLSRSQVWNSLDLLPGDWALRSARALLGV 161
+DDPLPN R NT I T+H VP GP VLSR QV N LDLL GD A R AR LL V
Sbjct: 266 SDDPLPNSRAR*NTTIRTVHGSPVPG*SGPFVLSRPQVRNPLDLLAGDRAFRPARPLLRV 87
Query: 160 LVHKFPTRCLDDAGLVRFGGVAVPLPV 80
LVH+ P+ LDD GLV GGVAVPLPV
Sbjct: 86 LVHELPSWGLDDPGLVGLGGVAVPLPV 6
Score = 109 bits (233), Expect = 4e-025
Identities = 53/86 (61%), Positives = 60/86 (69%)
Frame = +3 / +1
Query: 81 TGSGTATPPNRTRPASSRHLVGNLCTSTPRSARADRSAQSPGRRSKEFHT*DLLSTRGPG 260
TGSGTATPP+ TRP SSR G+ CTST R RA R+A+SP RRS+ F T* LLST+GP
Sbjct: 7 TGSGTATPPSPTRPGSSRPQEGSSCTSTRRRGRAGRNARSPARRSRGFLT*GLLSTKGPD 186
Query: 261 WQGTKGL*IVHMAVFCLVLQLGRGSS 338
+ GT * V M VF L LGRGSS
Sbjct: 187 YPGTGEP*TVLMVVFYLARLLGRGSS 264
>gb|CN817735.1|CN817735 HRO4461_E02_J03ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4461_E02_J03, mRNA sequence
Length = 683
Score = 223 bits (481), Expect = 3e-059
Identities = 92/96 (95%), Positives = 94/96 (97%)
Frame = +2 / +2
Query: 59 KMVQRLTYRKRHSYATKSNQTRVVKTPGGKLVYQYTKKRASGPKCPVTGKKIQGIPHLRP 238
KMVQRLTYRKRHSYATKSNQTRVVKTPGGKLVYQYTKKRASGPKCPVTGKKIQGIPHLRP
Sbjct: 44 KMVQRLTYRKRHSYATKSNQTRVVKTPGGKLVYQYTKKRASGPKCPVTGKKIQGIPHLRP 223
Query: 239 AEYKRSRLARNQRTVNRPYGGVLSGTAVRERIIRAF 346
EYKRSRL+RN+RTVNRPYGGVLSG AVRERIIRAF
Sbjct: 224 TEYKRSRLSRNRRTVNRPYGGVLSGPAVRERIIRAF 331
Score = 221 bits (476), Expect = 1e-058
Identities = 96/115 (83%), Positives = 98/115 (85%)
Frame = -3 / -3
Query: 405 LSLVFWIFNTFFTIFCSSTKKARMILSLTAVPDRTPPYGRFTVLWFLANLDLLYSAGLRC 226
LSLVFWIF TF TIFCSSTKKAR+ILSLTA P+ TPP GRFTVL FL NLDLLYS GLR
Sbjct: 390 LSLVFWIFRTFLTIFCSSTKKARIILSLTAGPESTPP*GRFTVLRFLDNLDLLYSVGLR* 211
Query: 225 GIPWIFFPVTGHFGPLARFLVYWYTSFPPGVLTTRVWFDLVA*LCRFR*VSRCTI 61
GIPWIFFPVTGH GPLA F VYW TSFPPGVLTTRV FD VA*L RFR*V RCTI
Sbjct: 210 GIPWIFFPVTGHLGPLALFFVYW*TSFPPGVLTTRV*FDFVA*L*RFR*VRRCTI 46
Score = 146 bits (314), Expect = 3e-036
Identities = 61/113 (53%), Positives = 80/113 (70%)
Frame = +1 / +1
Query: 61 DGAAAHLPEAAQLRHQIEPDPRRQDTWWETCVPVHQEARERTEVPSHREEDPRNSTPETC 240
DGAA+ LPEA+QLR ++E DP RQD W+ C+PVH+E R++VP + EEDP NS+PE
Sbjct: 46 DGAASDLPEASQLRDKVESDPGRQDPRWKACLPVHKEEG*RSQVPRYWEEDPGNSSPEAY 225
Query: 241 *VQEVQVGKEPKDCESSIWRCSVWYCS*GEDHPRLFG*GTEDSEEGVEDPKDQ 399
*VQEV+V EP+DCE S+W + W C *GED+ G ED EG E+P++Q
Sbjct: 226 *VQEVKVV*EPQDCEPSLWWSAFWTCC*GEDYSCFLGRRAEDC*EGPENPENQ 384
Score = 121 bits (259), Expect = 1e-028
Identities = 56/98 (57%), Positives = 70/98 (71%)
Frame = -2 / -2
Query: 352 NQKGADDPLPNCSTRQNTAIWTIHSPLVPCQPGPLVLSRSQVWNSLDLLPGDWALRSARA 173
+Q+ ++PLPN +R+++ I T+HSP VP QP PLVLSR QV NSLDLLP + AL +A
Sbjct: 337 DQESTNNPLPNSRSRKHSTIRTVHSPAVPRQP*PLVLSRPQVRNSLDLLPSNGALGTASP 158
Query: 172 LLGVLVHKFPTRCLDDAGLVRFGGVAVPLPVGEPLHHL 59
LL VLV+K T LDD GL+R VAV LPV + LHHL
Sbjct: 157 LLCVLVNKLSTWGLDDPGLIRLCRVAVTLPVSQTLHHL 44
>gb|CN816832.1|CN816832 HRO4525_C10_E19ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4525_C10_E19, mRNA sequence
Length = 544
Score = 216 bits (467), Expect = 2e-057
Identities = 91/108 (84%), Positives = 93/108 (86%)
Frame = -3 / -2
Query: 405 LSLVFWIFNTFFTIFCSSTKKARMILSLTAVPDRTPPYGRFTVLWFLANLDLLYSAGLRC 226
LSLVF IF TFFTIFCSST+KARMILSLTA P+ TPP GR TVL FL NLDLLYS GLR
Sbjct: 339 LSLVFCIFKTFFTIFCSSTRKARMILSLTAGPESTPP*GRLTVLRFLDNLDLLYSVGLRW 160
Query: 225 GIPWIFFPVTGHFGPLARFLVYWYTSFPPGVLTTRVWFDLVA*LCRFR 82
GIPWIFFPVTGH GPLA FLVYWYTS PPGVLTTRVW DLVA*LC FR
Sbjct: 159 GIPWIFFPVTGHLGPLALFLVYWYTSLPPGVLTTRVWLDLVA*LCLFR 16
Score = 204 bits (441), Expect = 8e-054
Identities = 84/88 (95%), Positives = 86/88 (97%)
Frame = +2 / +2
Query: 83 RKRHSYATKSNQTRVVKTPGGKLVYQYTKKRASGPKCPVTGKKIQGIPHLRPAEYKRSRL 262
RKRHSYATKSNQTRVVKTPGGKLVYQYTKKRASGPKCPVTGKKIQGIPHLRP EYKRSRL
Sbjct: 17 RKRHSYATKSNQTRVVKTPGGKLVYQYTKKRASGPKCPVTGKKIQGIPHLRPTEYKRSRL 196
Query: 263 ARNQRTVNRPYGGVLSGTAVRERIIRAF 346
+RN+RTVNRPYGGVLSG AVRERIIRAF
Sbjct: 197 SRNRRTVNRPYGGVLSGPAVRERIIRAF 280
Score = 126 bits (269), Expect = 4e-030
Identities = 53/107 (49%), Positives = 71/107 (66%)
Frame = +1 / +1
Query: 82 PEAAQLRHQIEPDPRRQDTWWETCVPVHQEARERTEVPSHREEDPRNSTPETC*VQEVQV 261
PE AQL HQ++PD RQD W VPVHQE R ++P REEDP +S PE VQ+++V
Sbjct: 16 PEEAQLCHQVQPDAGRQDPRW*ARVPVHQEEG*RPQMPCDREEDPGDSPPEAYRVQKIKV 195
Query: 262 GKEPKDCESSIWRCSVWYCS*GEDHPRLFG*GTEDSEEGVEDPKDQG 402
EP+DC+ S+W ++W CS* +DH G G ED EE +E+ ++QG
Sbjct: 196 V*EPQDCQPSLWWSALWTCS*RKDHSCFPGRGAEDCEESLENTENQG 336
Score = 106 bits (227), Expect = 3e-024
Identities = 48/90 (53%), Positives = 59/90 (65%)
Frame = -2 / -1
Query: 352 NQKGADDPLPNCSTRQNTAIWTIHSPLVPCQPGPLVLSRSQVWNSLDLLPGDWALRSARA 173
+Q+ +DP NC +R+++ I T+ SP VP QP VL R QV N LDLLPG A +A
Sbjct: 286 DQESTNDPFSNCRSREHSTIGTVDSPAVPRQP*SFVLGRPQVGNPLDLLPGHRAFGAASP 107
Query: 172 LLGVLVHKFPTRCLDDAGLVRFGGVAVPLP 83
LLGVLVH+ T LDD LV GG+AVPLP
Sbjct: 106 LLGVLVHELTTWGLDDPRLVGLGGIAVPLP 17
>gb|CN821505.1|CN821505 HRO4426_B01_C02ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4426_B01_C02, mRNA
sequence
Length = 459
Score = 157 bits (337), Expect = 2e-039
Identities = 66/79 (83%), Positives = 68/79 (86%)
Frame = -3 / -1
Query: 405 LSLVFWIFNTFFTIFCSSTKKARMILSLTAVPDRTPPYGRFTVLWFLANLDLLYSAGLRC 226
LSLVF IF TFFTIFCSST+KARMILSLTA P+ TPPYGR TVL FL NLDLLYS GLR
Sbjct: 246 LSLVFCIFKTFFTIFCSSTRKARMILSLTAGPESTPPYGRLTVLRFLDNLDLLYSVGLRW 67
Query: 225 GIPWIFFPVTGHFGPLARF 169
GIPWIFFPVTGH GPLA F
Sbjct: 66 GIPWIFFPVTGHLGPLALF 10
Score = 135 bits (289), Expect = 8e-033
Identities = 55/59 (93%), Positives = 57/59 (96%)
Frame = +2 / +2
Query: 170 KRASGPKCPVTGKKIQGIPHLRPAEYKRSRLARNQRTVNRPYGGVLSGTAVRERIIRAF 346
KRASGPKCPVTGKKIQGIPHLRP EYKRSRL+RN+RTVNRPYGGVLSG AVRERIIRAF
Sbjct: 11 KRASGPKCPVTGKKIQGIPHLRPTEYKRSRLSRNRRTVNRPYGGVLSGPAVRERIIRAF 187
>gb|CN820354.1|CN820354 HRO4418_D08_G16ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4418_D08_G16, mRNA
sequence
Length = 546
Score = 150 bits (322), Expect = 2e-037
Identities = 64/77 (83%), Positives = 65/77 (84%)
Frame = -3 / -2
Query: 405 LSLVFWIFNTFFTIFCSSTKKARMILSLTAVPDRTPPYGRFTVLWFLANLDLLYSAGLRC 226
LSLVFWIF TF TIFCSSTKKARMILSLTA P+ TPP GRFTVL FL NLDLLYS GLR
Sbjct: 233 LSLVFWIFRTFLTIFCSSTKKARMILSLTAGPESTPP*GRFTVLRFLDNLDLLYSVGLR* 54
Query: 225 GIPWIFFPVTGHFGPLA 175
GIPWIFFPVT H GP A
Sbjct: 53 GIPWIFFPVTEHLGPSA 3
Score = 125 bits (267), Expect = 8e-030
Identities = 51/57 (89%), Positives = 53/57 (92%)
Frame = +2 / +1
Query: 176 ASGPKCPVTGKKIQGIPHLRPAEYKRSRLARNQRTVNRPYGGVLSGTAVRERIIRAF 346
A GPKC VTGKKIQGIPHLRP EYKRSRL+RN+RTVNRPYGGVLSG AVRERIIRAF
Sbjct: 4 AEGPKCSVTGKKIQGIPHLRPTEYKRSRLSRNRRTVNRPYGGVLSGPAVRERIIRAF 174
>gb|CN815372.1|CN815372 HRO4508_H11_P22ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4508_H11_P22, mRNA sequence
Length = 720
Score = 136 bits (292), Expect = 3e-033
Identities = 58/68 (85%), Positives = 59/68 (86%)
Frame = -3 / -2
Query: 405 LSLVFWIFNTFFTIFCSSTKKARMILSLTAVPDRTPPYGRFTVLWFLANLDLLYSAGLRC 226
LSLVFWIF TF TIFCSSTKKARMILSLTA P+ TPP GRFTVL FL NLDLLYS GLR
Sbjct: 440 LSLVFWIFRTFLTIFCSSTKKARMILSLTAGPESTPP*GRFTVLRFLDNLDLLYSVGLR* 261
Query: 225 GIPWIFFP 202
GIPWIFFP
Sbjct: 260 GIPWIFFP 237
Score = 108 bits (230), Expect = 1e-024
Identities = 44/50 (88%), Positives = 48/50 (96%)
Frame = +2 / +1
Query: 197 VTGKKIQGIPHLRPAEYKRSRLARNQRTVNRPYGGVLSGTAVRERIIRAF 346
++GKKIQGIPHLRP EYKRSRL+RN+RTVNRPYGGVLSG AVRERIIRAF
Sbjct: 232 LSGKKIQGIPHLRPTEYKRSRLSRNRRTVNRPYGGVLSGPAVRERIIRAF 381
>gb|CN814739.1|CN814739 HRO4502_A01_A02ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4502_A01_A02, mRNA sequence
Length = 444
Score = 124 bits (266), Expect(2) = 7e-032
Identities = 53/64 (82%), Positives = 55/64 (85%)
Frame = -3 / -1
Query: 405 LSLVFWIFNTFFTIFCSSTKKARMILSLTAVPDRTPPYGRFTVLWFLANLDLLYSAGLRC 226
LSLVF IF TFFTIFCSST+KARMILSLTA P+ TPPYGR TVL FL NLDLLYS GLR
Sbjct: 219 LSLVFCIFKTFFTIFCSSTRKARMILSLTAGPESTPPYGRLTVLRFLDNLDLLYSVGLRW 40
Query: 225 GIPW 214
GIPW
Sbjct: 39 GIPW 28
Score = 99.6 bits (211), Expect = 4e-022
Identities = 41/53 (77%), Positives = 46/53 (86%)
Frame = +2 / +2
Query: 188 KCPVTGKKIQGIPHLRPAEYKRSRLARNQRTVNRPYGGVLSGTAVRERIIRAF 346
+ P ++ QGIPHLRP EYKRSRL+RN+RTVNRPYGGVLSG AVRERIIRAF
Sbjct: 2 RVPCDWEEDQGIPHLRPTEYKRSRLSRNRRTVNRPYGGVLSGPAVRERIIRAF 160
Score = 27.2 bits (53), Expect(2) = 7e-032
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = -3 / -3
Query: 225 GIPWIFFPVTGH 190
G P +FFPVTGH
Sbjct: 40 GNPLVFFPVTGH 5
>gb|CN820073.1|CN820073 HRO4410_G08_M16ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4410_G08_M16, mRNA
sequence
Length = 384
Score = 108 bits (230), Expect = 1e-024
Identities = 48/58 (82%), Positives = 50/58 (86%)
Frame = -3 / -3
Query: 405 LSLVFWIFNTFFTIFCSSTKKARMILSLTAVPDRTPPYGRFTVLWFLANLDLLYSAGL 232
LSLVF IF TFFTIFCSST+KARMILSLTA P+ TPP GRFTVL FL NLDLLYS GL
Sbjct: 175 LSLVFCIFKTFFTIFCSSTRKARMILSLTAGPESTPP*GRFTVLRFLDNLDLLYSVGL 2
>gb|CN820934.1|CN820934 HRO4421_F07_K13ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4421_F07_K13, mRNA
sequence
Length = 395
Score = 95.9 bits (203), Expect = 6e-021
Identities = 43/53 (81%), Positives = 45/53 (84%)
Frame = -3 / -3
Query: 405 LSLVFWIFNTFFTIFCSSTKKARMILSLTAVPDRTPPYGRFTVLWFLANLDLL 247
LSLVF IF TFFTIFCSST+KARMILSLTA P+ TPP GR TVL FL NLDLL
Sbjct: 171 LSLVFCIFKTFFTIFCSSTRKARMILSLTAGPESTPP*GRLTVLRFLDNLDLL 13
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,304,723
Number of Sequences: 8143
Number of extensions: 102597
Number of successful extensions: 1468
Number of sequences better than 1.0e-020: 9
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 210782732
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)