TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2192958.2.1
(1629 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN180781.1|CN180781 As03_isu1961_C ISU cDNA from oat Ave... 360 e-159
>gb|CN180781.1|CN180781 As03_isu1961_C ISU cDNA from oat Avena sativa cDNA clone
As03_isu1961, mRNA sequence
Length = 1004
Score = 360 bits (780), Expect(2) = e-159
Identities = 144/169 (85%), Positives = 156/169 (92%)
Frame = -1 / -1
Query: 870 EADEAPDTTTGDIIFVLQQKEHPKFKRKGDDLFYEHTLTLTESLCGFQFVLTHLDNRQLL 691
EADEAPDT TGDIIFVLQQKEHPKFKRKGDDLFYEHTLTLTE+LCGFQ+VLTHLD RQLL
Sbjct: 674 EADEAPDTVTGDIIFVLQQKEHPKFKRKGDDLFYEHTLTLTEALCGFQYVLTHLDGRQLL 495
Query: 690 IKSNPGEVVKPDSFKAINDEGMPMYQRPFMKGKLYIHFSVEFPDSLSPEQCKALEVVLPP 511
IKSNPGEVVKPDSFKAINDEGMPMYQRPFMKGKLYIHF+V+FPDSL+P+QCKALE VLPP
Sbjct: 494 IKSNPGEVVKPDSFKAINDEGMPMYQRPFMKGKLYIHFTVDFPDSLNPDQCKALETVLPP 315
Query: 510 KPVSQYTDMELDECEETMTYDVNIEEEMRRRQQHHQEAYDEDDDVPGGG 364
KPVSQYTDMELDECEETM YD++IEEEMRRRQQ + ++D+ GG
Sbjct: 314 KPVSQYTDMELDECEETMAYDIDIEEEMRRRQQQQAQEAYDEDEDMPGG 168
Score = 218 bits (470), Expect(2) = e-159
Identities = 88/115 (76%), Positives = 97/115 (84%)
Frame = -1 / -1
Query: 1233 DLYNGTSKKLSLSRSVLCSKCNGKGSKSGASSRCAGCQGSGFKVQIRQLGPGMIQQMQHP 1054
+LYNGTSKKLSLSR+VLCSKCNGKGSKSGAS +CAGCQG+G+KVQIRQLGPGMIQQMQ P
Sbjct: 1004 ELYNGTSKKLSLSRNVLCSKCNGKGSKSGASMKCAGCQGAGYKVQIRQLGPGMIQQMQQP 825
Query: 1053 CNECKGSGETISDKDRCPQCKGDXXXXXXXXXXXXXXKGMQNGQKITFPGEADEA 889
CNEC+G+GETISDKDRC QCKG+ KGMQ+ QKITFPGEADEA
Sbjct: 824 CNECRGTGETISDKDRCGQCKGEKVVQEKKVLEVVVEKGMQHSQKITFPGEADEA 660
Score = 208 bits (448), Expect(3) = e-109
Identities = 92/144 (63%), Positives = 96/144 (66%)
Frame = +2 / +1
Query: 410 CCCRLRISSSMFTXXXXXXXXXXXXXXXYCDTGLGGRTTSRALHCSGLNESGNSTEK*MY 589
CCCRL ISSSM YCDTGLGGRT SRALH SG +ESG ST K M
Sbjct: 214 CCCRLLISSSMSMSYAIVSSHSSSSMSVYCDTGLGGRTVSRALHWSGFSESGKSTVKWM* 393
Query: 590 SLPFMKGL*YMGMPSSLIALKESGFTTSPGFDLISSCLLSK*VRTNWKPHRDXXXXXXXX 769
SLPFM GL YMGMPSSLIAL ESG TTSPG DL+SSCL SK*VRT WKPHR
Sbjct: 394 SLPFMNGLWYMGMPSSLIALNESGLTTSPGLDLMSSCLPSK*VRTYWKPHRASVRVRVCS 573
Query: 770 XXXXXPFLLNLGCSFCWRTKMISP 841
PF LNLGCSFC RTK++SP
Sbjct: 574 *KRSSPFRLNLGCSFCCRTKIMSP 645
Score = 182 bits (393), Expect(3) = 3e-091
Identities = 82/134 (61%), Positives = 95/134 (70%)
Frame = -3 / -3
Query: 853 *YYHWRYHLCSPAEGASQVQEKGR*PLLRAHTDLD*ISVWLPVCSDSLG*QAAADQIEPW 674
*Y +W ++L AEGASQVQ +GR PLL AH+D * VWLP+CS S G QAAA Q++ W
Sbjct: 657 *YRYWGHNLRPTAEGASQVQAEGRRPLL*AHSDPH*SPVWLPICSYSFGRQAAAHQVQSW 478
Query: 673 *SCEA*FFQGNQR*RHAHVSEALHEGQAVHSLFGGVPGLVEPGAVQGPGGCSPAQASITV 494
SC A* QG+QR HAHV EA+HEGQA+H L GG+PGL EPG VQGP C AQA + V
Sbjct: 477 GSC*A*LVQGDQRRGHAHVPEAVHEGQALHPLHGGLPGLAEPGPVQGPRDCPAAQARVAV 298
Query: 493 H*HGARRVRGDNDV 452
H HGAR V GD V
Sbjct: 297 HGHGAR*V*GDYGV 256
Score = 179 bits (385), Expect(3) = e-109
Identities = 76/113 (67%), Positives = 77/113 (68%)
Frame = +2 / +1
Query: 890 ASSASPGNVIFCPFCIPXXXXXXXXXXXXXXLSPLHCGHLSLSLMVSPEPLHSLQGXXXX 1069
ASSASPG VIF CIP SPLHC HLSLSLMVSP PLHSLQG
Sbjct: 661 ASSASPGKVIFWLCCIPFSTTTSRTFFSCTTFSPLHCPHLSLSLMVSPVPLHSLQGCCIC 840
Query: 1070 XXXPGPNCRIWTLKPEPWQPAHLDEAPDFEPLPLHLEQRTLRDRESFFDVPLY 1228
PGPN RIWTL P PWQPAH EAPDFEPLPLH EQ TLR+RESF DVPLY
Sbjct: 841 *IIPGPNWRIWTL*PAPWQPAHFIEAPDFEPLPLHFEQSTLRERESFLDVPLY 999
Score = 150 bits (322), Expect(5) = 4e-076
Identities = 60/92 (65%), Positives = 80/92 (86%)
Frame = +1 / +3
Query: 511 GRENNLQGLALLRAQRVRELHRKVNVQLALHEGPLIHGHAFIVDCLERIRLHNFTRVRFD 690
GR+++L GLAL+R QRVRE+HR+V+V+LALHE PL+HGHA +VD LER+RL+NF R+ D
Sbjct: 315 GRQDSL*GLALVRVQRVREVHREVDVELALHERPLVHGHALVVDRLERVRLNNFPRIGLD 494
Query: 691 QQLPVIQVSQNKLEATQRFSQGQCVLVEEVIA 786
+QLP +Q+S+N LEATQ FS+GQ VL+EEV+A
Sbjct: 495 EQLPAVQMSKNILEATQGFSEGQSVLIEEVVA 590
Score = 140 bits (300), Expect(3) = 3e-091
Identities = 65/109 (59%), Positives = 71/109 (65%)
Frame = -3 / -3
Query: 1228 VQWHIKEALSVPQCSLLQVQW*GFKVWSFIEVCWLPRFWL*GPNPAVGAWNDSANAASLQ 1049
VQWHI EA S QC+LL+VQW G KVW F EVC LP L*GPN VG NDSANAA+LQ
Sbjct: 999 VQWHI*EAFSFTQCALLKVQWQGLKVWGFDEVCRLPGSRL*GPNTPVGTRNDSANAATLQ 820
Query: 1048 *VQGFWRDHQR*G*MPTMQR**SCAXXXXXXXXXXERYAERAEDHIPWR 902
*VQG W DHQR G*M +Q CA E YA + EDH+PWR
Sbjct: 819 *VQGNW*DHQRQG*MWAVQGREGCAREEGSGGSGREGYAAQPEDHLPWR 673
Score = 127 bits (272), Expect(5) = 4e-076
Identities = 52/79 (65%), Positives = 57/79 (72%)
Frame = +3 / +2
Query: 993 CIVGIYPYR*WSLQNPCTHCKDAAFAESFQAPTAGFGP*SQNLGNQHTSMKLQTLNPYHC 1172
C IYP R*WS Q PCTHCK AAFAESF PT FGP ++ GN+HTS K QTL+P HC
Sbjct: 764 CTAHIYPCR*WSHQFPCTHCKVAAFAESFLVPTGVFGPYNRLPGNRHTSSKPQTLSPCHC 943
Query: 1173 TWSREHCGTERASLMCHCT 1229
T SR HC E+AS MCHCT
Sbjct: 944 TLSRAHCVKEKAS*MCHCT 1000
Score = 112 bits (240), Expect(3) = 1e-051
Identities = 57/96 (59%), Positives = 63/96 (65%)
Frame = +3 / +2
Query: 585 CTACPS*RASDTWACLHR*LP*KNQASQLHQGSI*SAAACYPSESEQTGSHTEIQSRSVC 764
C ACPS* AS TWAC R* P* +QA QL Q * AAAC P+E E GSHT +Q S C
Sbjct: 389 CRACPS*TASGTWACPRR*SP*TSQA*QLPQDWT**AAACRPNE*EHIGSHTGLQ*GSEC 568
Query: 765 ARRRGHRPFS*TWDAPSAGEQR*YLQW*YQVLHQLR 872
A RRG RP +*TWDAPSA +R Q YQ LH+ R
Sbjct: 569 AHRRGRRPSA*TWDAPSAVGRRLCPQ*RYQALHRPR 676
Score = 97.3 bits (206), Expect(3) = 1e-051
Identities = 44/72 (61%), Positives = 53/72 (73%)
Frame = +1 / +3
Query: 892 FISFARECDLLPVLHTFLNYHFKNFLLRHNFITFALWASILIADGLSRTLALIARMLHLL 1071
FI ARE DLL VLHT L+++ +N LL HN + AL SIL+ADGL+ + ALIAR+LHLL
Sbjct: 663 FIGLAREGDLLAVLHTLLDHYLQNLLLLHNLLALALPTSILVADGLTSSPALIARLLHLL 842
Query: 1072 NHSRPQLPDLDL 1107
NHS QL LDL
Sbjct: 843 NHSWSQLAYLDL 878
Score = 92.7 bits (196), Expect(4) = 3e-041
Identities = 48/80 (60%), Positives = 53/80 (66%)
Frame = -2 / -2
Query: 1229 CTMAHQRSSLCPAVFSAPSAMVRVQSLELHRGVLVAKVLALRSKSGSWGLE*FSKCSILA 1050
CTMAH RS L A+ SA SAM R QSL L V VA+ +RSK SW E*FSKCS LA
Sbjct: 1000 CTMAHLRSFLFHAMCSAQSAMARAQSLGLR*SVPVAREPVIRSKYASWDQE*FSKCSNLA 821
Query: 1049 MSARVLERPSAIRIDAHNAK 990
MSA L RPSA RID +A+
Sbjct: 820 MSAGELVRPSATRIDVGSAR 761
Score = 81.7 bits (172), Expect(4) = 3e-041
Identities = 41/67 (61%), Positives = 46/67 (68%)
Frame = -2 / -2
Query: 785 AMTSSTSTH*P*LNLCVASSLF*LTWITGSC*SNRTLVKL*SLILSRQSTMKACPCIRGP 606
A TSS ST *P L CVAS++F L W GSC S+ L KL SL SR+ST +ACPC RG
Sbjct: 589 ATTSSMSTL*PSLKPCVASNMFLLIWTAGSCSSSPILGKLLSLTRSRRSTTRACPCTRGR 410
Query: 605 S*RASCT 585
S*RAS T
Sbjct: 409 S*RASST 389
Score = 47.8 bits (98), Expect(3) = 3e-091
Identities = 18/25 (72%), Positives = 21/25 (84%)
Frame = -1 / -1
Query: 438 EEEMRRRQQHHQEAYDEDDDVPGGG 364
EE RR+QQ QEAYDED+D+PGGG
Sbjct: 239 EEMRRRQQQQAQEAYDEDEDMPGGG 165
Score = 45.1 bits (92), Expect(3) = e-109
Identities = 14/33 (42%), Positives = 17/33 (51%)
Frame = +2 / +1
Query: 332 AAYCWAHCTLCPPPGTXXXXXXXXWWCCCRLRI 430
++YCWAHCT PPP W CC R+
Sbjct: 130 SSYCWAHCTRWPPPPGMSSSSS*ASWACCCCRL 228
Score = 29.5 bits (58), Expect(5) = 4e-076
Identities = 10/25 (40%), Positives = 11/25 (44%)
Frame = +2 / +1
Query: 365 PPPGTXXXXXXXXWWCCCRLRISSS 439
PPPG W CCC + SS
Sbjct: 166 PPPGMSSSSS*ASWACCCCRLLISS 240
Score = 28.6 bits (56), Expect(4) = 3e-041
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = -1 / -1
Query: 450 DVNIEEEMRRRQQHHQEAYDEDDDVPGGGQRV 355
D+ E R++QQ + +++D GGGQRV
Sbjct: 248 DIEEEMRRRQQQQAQEAYDEDEDMPGGGGQRV 153
Score = 27.6 bits (54), Expect(3) = 1e-051
Identities = 14/21 (66%), Positives = 17/21 (80%)
Frame = +1 / +3
Query: 1168 IALGAENTAGQRELL*CAIVQ 1230
IAL AE+ A +R+LL CAIVQ
Sbjct: 939 IAL*AEHIA*KRKLLRCAIVQ 1001
Score = 26.7 bits (52), Expect(5) = 4e-076
Identities = 9/15 (60%), Positives = 9/15 (60%)
Frame = +3 / +2
Query: 480 APCQCTVILAWAGEQ 524
APC CT AWA Q
Sbjct: 284 APCPCTATRAWAAGQ 328
Score = 26.3 bits (51), Expect(4) = 3e-041
Identities = 11/17 (64%), Positives = 13/17 (76%)
Frame = -2 / -2
Query: 938 VCRTGRRSHSLAKLMKQ 888
VC T RRS SLA+ MK+
Sbjct: 709 VCSTARRSPSLARPMKR 659
Score = 22.6 bits (43), Expect(5) = 4e-076
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = +3 / +2
Query: 891 LHQLRQGM*SSARSAY 938
LH+ RQG *SS +AY
Sbjct: 662 LHRPRQGR*SSGCAAY 709
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 16,937,466
Number of Sequences: 8143
Number of extensions: 232625
Number of successful extensions: 1067
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 45
effective length of database: 1,201,052
effective search space used: 596922844
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)