TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2188214.2.1
(880 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816242.1|CN816242 HRO4518_E06_I12ZS5 Lib AA071E1X Aven... 149 4e-037
gb|CN816396.1|CN816396 HRO4520_C05_F10ZS5 Lib AA071E1X Aven... 149 4e-037
>gb|CN816242.1|CN816242 HRO4518_E06_I12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4518_E06_I12, mRNA sequence
Length = 723
Score = 149 bits (321), Expect = 4e-037
Identities = 60/99 (60%), Positives = 74/99 (74%)
Frame = -1 / +3
Query: 871 DXXNXVXXXPCXHTIHVKCLKXXXXXCQFACPLCSKSVCDMSKAWERLDMELATLSDSCD 692
D + V C HTIH CLK + CPLCSKSVCDMS+AWERLD E+A L DS D
Sbjct: 81 DSIDGVSVLRCGHTIHENCLKEMEKHRKLICPLCSKSVCDMSRAWERLDREIARLPDSYD 260
Query: 691 HKMVRILCNDCGAISDVQFHLIAHKCQNCKSYNTRQI*S 575
+MVRILCN+C AI++V+F+LIA KCQ+C+SYNTR+I*+
Sbjct: 261 GRMVRILCNECEAIAEVRFNLIALKCQSCQSYNTRRI*N 377
Score = 111 bits (238), Expect = 1e-025
Identities = 53/84 (63%), Positives = 59/84 (70%)
Frame = +3 / -3
Query: 582 IWRVLYDLQFWHLCAIKWNCTSDIAPQSLHNMRTIL*SQESDRVASSMSSLSHAFDMSQT 761
I RVLYD Q WHL AIK TS IA SLHNMRTIL S ES +A S+SSLSHA DMS T
Sbjct: 370 IRRVLYDWQLWHLSAIKLKRTSAIASHSLHNMRTILPS*ESGSLAISLSSLSHALDMSHT 191
Query: 762 DLEQRGHANWQXXXIXFRHFT*MV 833
DLEQ GH N + I F+ F+*+V
Sbjct: 190 DLEQSGHINLRCFSISFKQFS*IV 119
Score = 109 bits (232), Expect = 8e-025
Identities = 48/100 (48%), Positives = 54/100 (54%)
Frame = +1 / -1
Query: 574 YFKSGGCCMTCNSGTCAQSNGTAHLILPRSRCIICGPFCDHRSQTELPAPCRAFPMPSTC 753
YF+S CCMT NSGT AQS+ A L+LP CIIC PF HRS L PC PMP TC
Sbjct: 378 YFRSAECCMTGNSGT*AQSS*NAPLLLPHIHCIICEPFYHHRSLAVLLFPCPVSPMPLTC 199
Query: 754 HKPTWSKGGMQTGSXXXXLSGTLHEWYXXXAXXXXHXXXR 873
H TWS+ G+ T LS + HE Y A R
Sbjct: 198 HILTWSRAGILTCDVSPFLSSSFHELYDHTAKQTHRLSSR 79
Score = 98.7 bits (209), Expect = 1e-021
Identities = 51/100 (51%), Positives = 59/100 (59%)
Frame = -3 / +2
Query: 872 RXXKXCXXXAXXXYHSCKVPEXNXXXLPVCMPPLLQVGL*HVEGMGKARHGAGNSV*LL* 693
R + C A Y+S K+ E N V MP LLQV +*HV+GMG+ G + LL*
Sbjct: 80 RLDRRCVCFAVWSYNS*KLLERNGETSQVNMPALLQVSM*HVKGMGETGQGNSKTARLL* 259
Query: 692 SQNGPHIMQRLRGNIRCAVPFDCAQVPELQVIQHPPDLK* 573
NG HIMQ + GN R A DCAQVPEL VIQH DLK*
Sbjct: 260 W*NGSHIMQ*M*GNSRGAFQLDCAQVPELPVIQHSADLK* 379
>gb|CN816396.1|CN816396 HRO4520_C05_F10ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4520_C05_F10, mRNA sequence
Length = 851
Score = 149 bits (321), Expect = 4e-037
Identities = 60/99 (60%), Positives = 74/99 (74%)
Frame = -1 / +2
Query: 871 DXXNXVXXXPCXHTIHVKCLKXXXXXCQFACPLCSKSVCDMSKAWERLDMELATLSDSCD 692
D + V C HTIH CLK + CPLCSKSVCDMS+AWERLD E+A L DS D
Sbjct: 524 DSIDGVSVLRCGHTIHENCLKEMEKHRKLICPLCSKSVCDMSRAWERLDREIARLPDSYD 703
Query: 691 HKMVRILCNDCGAISDVQFHLIAHKCQNCKSYNTRQI*S 575
+MVRILCN+C AI++V+F+LIA KCQ+C+SYNTR+I*+
Sbjct: 704 GRMVRILCNECEAIAEVRFNLIALKCQSCQSYNTRRI*N 820
Score = 111 bits (238), Expect = 1e-025
Identities = 53/84 (63%), Positives = 59/84 (70%)
Frame = +3 / -3
Query: 582 IWRVLYDLQFWHLCAIKWNCTSDIAPQSLHNMRTIL*SQESDRVASSMSSLSHAFDMSQT 761
I RVLYD Q WHL AIK TS IA SLHNMRTIL S ES +A S+SSLSHA DMS T
Sbjct: 813 IRRVLYDWQLWHLSAIKLKRTSAIASHSLHNMRTILPS*ESGSLAISLSSLSHALDMSHT 634
Query: 762 DLEQRGHANWQXXXIXFRHFT*MV 833
DLEQ GH N + I F+ F+*+V
Sbjct: 633 DLEQSGHINLRCFSISFKQFS*IV 562
Score = 109 bits (232), Expect = 8e-025
Identities = 48/100 (48%), Positives = 54/100 (54%)
Frame = +1 / -1
Query: 574 YFKSGGCCMTCNSGTCAQSNGTAHLILPRSRCIICGPFCDHRSQTELPAPCRAFPMPSTC 753
YF+S CCMT NSGT AQS+ A L+LP CIIC PF HRS L PC PMP TC
Sbjct: 821 YFRSAECCMTGNSGT*AQSS*NAPLLLPHIHCIICEPFYHHRSLAVLLFPCPVSPMPLTC 642
Query: 754 HKPTWSKGGMQTGSXXXXLSGTLHEWYXXXAXXXXHXXXR 873
H TWS+ G+ T LS + HE Y A R
Sbjct: 641 HILTWSRAGILTCDVSPFLSSSFHELYDHTAKQTHRLSSR 522
Score = 98.7 bits (209), Expect = 1e-021
Identities = 51/100 (51%), Positives = 59/100 (59%)
Frame = -3 / +1
Query: 872 RXXKXCXXXAXXXYHSCKVPEXNXXXLPVCMPPLLQVGL*HVEGMGKARHGAGNSV*LL* 693
R + C A Y+S K+ E N V MP LLQV +*HV+GMG+ G + LL*
Sbjct: 523 RLDRRCVCFAVWSYNS*KLLERNGETSQVNMPALLQVSM*HVKGMGETGQGNSKTARLL* 702
Query: 692 SQNGPHIMQRLRGNIRCAVPFDCAQVPELQVIQHPPDLK* 573
NG HIMQ + GN R A DCAQVPEL VIQH DLK*
Sbjct: 703 W*NGSHIMQ*M*GNSRGAFQLDCAQVPELPVIQHSADLK* 822
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 10,431,302
Number of Sequences: 8143
Number of extensions: 172906
Number of successful extensions: 1157
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 303117162
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)