TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2169937.2.1
(891 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF155932.1| Avena sativa 1,3-beta glucanase (Oglc13) mRN... 215 1e-067
gb|AY277385.1| Avena sativa 1,3-beta glucanase (Oglc13) gen... 217 2e-067
gb|CN821029.1|CN821029 HRO4422_G03_M06ZS5 Lib AA069E1X Aven... 171 2e-043
emb|Z15131.1|ASBGLUCAN A.sativa mRNA for beta glucanase 125 7e-036
>gb|AF155932.1| Avena sativa 1,3-beta glucanase (Oglc13) mRNA, partial cds
Length = 1045
Score = 215 bits (465), Expect(3) = 1e-067
Identities = 90/120 (75%), Positives = 100/120 (83%)
Frame = +2 / +1
Query: 338 FQPGTTVRDNGNGLNYNNLFDAMVDAVVAALEKAGAPNVRVVVSEXGWPSAGGFGASVDN 517
F+PGTTVRD+ +GL Y NLFDAMVDAV AALEKAGA +V+VVVSE GWP GG GASVDN
Sbjct: 556 FRPGTTVRDSKSGLTYTNLFDAMVDAVHAALEKAGAASVKVVVSESGWPKEGGTGASVDN 735
Query: 518 ARKYNQGLIDHVGRGTPKRTGPLETFVFAMFNENQKGGDPTEKNFGLFYGNKQPVYPIRF 697
AR YNQGLIDHVG GTPK+ G LET+VFAMF+ENQK G TEK+FGLF +K P YPIRF
Sbjct: 736 ARAYNQGLIDHVGGGTPKKRGALETYVFAMFDENQKTGAGTEKHFGLFNPDKSPAYPIRF 915
Score = 127 bits (272), Expect(3) = 5e-035
Identities = 61/119 (51%), Positives = 65/119 (54%)
Frame = -1 / -3
Query: 693 RIGYTGCLLP*KRPKFFSVGSPPFWFSLNMAKTKVSSGPVLLGVPRPTWSMSPWLYFRAL 514
R+G G L PK FSV +P FWFS NMA T VSS P LGVP PTWS+SPWLY AL
Sbjct: 911 RMG*AGDLSGLNSPKCFSVPAPVFWFSSNMANTYVSSAPRFLGVPPPTWSISPWLYALAL 732
Query: 513 STLAPKPPAEGQPXSDTTTLTLGXXXXXXXXXXXXXXXSNXXXXXXXXXXXLTVVPGWN 337
STLAP PP+ G P SDTTT TL SN TVVPG N
Sbjct: 731 STLAPVPPSFGHPLSDTTTFTLAAPAFSSAACTASTIASNRFV*VSPLLLSRTVVPGRN 555
Score = 52.8 bits (109), Expect(3) = 1e-067
Identities = 22/51 (43%), Positives = 26/51 (50%)
Frame = +1 / +1
Query: 169 FDVVANFVPAXKGSFGKGTCGTSRATWRAXGXRXSPNVYPYFAYRDNPRDI 321
FD VAN P G F + + G N+YPYF+YRDNPRDI
Sbjct: 385 FDAVANSFPPSAGVFAQSYMTDVARLLASTGAPLLANIYPYFSYRDNPRDI 537
Score = 27.6 bits (54), Expect(3) = 5e-035
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = -3 / -1
Query: 319 CRAGCPGTRSKG 284
C AGC GTRS+G
Sbjct: 535 CHAGCRGTRSRG 500
Score = 25.8 bits (50), Expect(3) = 5e-035
Identities = 14/28 (50%), Positives = 15/28 (53%)
Frame = -3 / -3
Query: 286 GTRLAXXGXPVPAK*RATSRMYPSRRTP 203
G LA G PV A+ RATS M TP
Sbjct: 503 G*MLASSGAPVLARSRATSVM*DCANTP 420
Score = 22.1 bits (42), Expect(3) = 1e-067
Identities = 7/9 (77%), Positives = 8/9 (88%)
Frame = +1 / +3
Query: 328 VRHVPAGHH 354
+R VPAGHH
Sbjct: 546 LRDVPAGHH 572
>gb|AY277385.1| Avena sativa 1,3-beta glucanase (Oglc13) gene, complete cds
Length = 3281
Score = 217 bits (469), Expect(3) = 2e-067
Identities = 90/120 (75%), Positives = 99/120 (82%)
Frame = +2 / +2
Query: 338 FQPGTTVRDNGNGLNYNNLFDAMVDAVVAALEKAGAPNVRVVVSEXGWPSAGGFGASVDN 517
F+PGTTVRD+ +GL Y NLFDAMVDAV AALEKAGAP V+VVVSE GWP GG GAS DN
Sbjct: 2801 FRPGTTVRDSKSGLTYTNLFDAMVDAVHAALEKAGAPGVKVVVSESGWPKTGGTGASTDN 2980
Query: 518 ARKYNQGLIDHVGRGTPKRTGPLETFVFAMFNENQKGGDPTEKNFGLFYGNKQPVYPIRF 697
AR YNQGLIDHVG GTPK+ G LET+VFAMF+ENQK G TEK+FGLF +K P YPIRF
Sbjct: 2981 ARAYNQGLIDHVGGGTPKKRGALETYVFAMFDENQKTGAGTEKHFGLFNPDKSPAYPIRF 3160
Score = 128 bits (275), Expect(3) = 6e-035
Identities = 60/119 (50%), Positives = 64/119 (53%)
Frame = -1 / -3
Query: 693 RIGYTGCLLP*KRPKFFSVGSPPFWFSLNMAKTKVSSGPVLLGVPRPTWSMSPWLYFRAL 514
R+GY G L PK FSV +P FWFS N+A T VSS P LGVP PTWS+SPWLY AL
Sbjct: 3156 RMGYAGDLSGLNSPKCFSVPAPVFWFSSNIANTYVSSAPRFLGVPPPTWSISPWLYALAL 2977
Query: 513 STLAPKPPAEGQPXSDTTTLTLGXXXXXXXXXXXXXXXSNXXXXXXXXXXXLTVVPGWN 337
S LAP PP G P SDTTT T G SN TVVPG N
Sbjct: 2976 SVLAPVPPVLGHPLSDTTTFTPGAPAFSSAACTASTIASNRFVYVSPLLLSRTVVPGRN 2800
Score = 50.6 bits (104), Expect(3) = 2e-067
Identities = 21/51 (41%), Positives = 26/51 (50%)
Frame = +1 / +2
Query: 169 FDVVANFVPAXKGSFGKGTCGTSRATWRAXGXRXSPNVYPYFAYRDNPRDI 321
FD VA+ P G F + + G N+YPYF+YRDNPRDI
Sbjct: 2630 FDAVASSFPPSAGVFAQSYMTDVARLLASTGAPLLANIYPYFSYRDNPRDI 2782
Score = 26.7 bits (52), Expect(3) = 6e-035
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = -3 / -1
Query: 319 CRAGCPGTRSKG 284
C AGC GTRS+G
Sbjct: 2780 CPAGCRGTRSRG 2745
Score = 24.9 bits (48), Expect(3) = 6e-035
Identities = 14/28 (50%), Positives = 14/28 (50%)
Frame = -3 / -3
Query: 286 GTRLAXXGXPVPAK*RATSRMYPSRRTP 203
G LA G PV A RATS M TP
Sbjct: 2748 G*MLASSGAPVLASSRATSVM*DCANTP 2665
Score = 22.1 bits (42), Expect(3) = 2e-067
Identities = 7/9 (77%), Positives = 8/9 (88%)
Frame = +1 / +1
Query: 328 VRHVPAGHH 354
+R VPAGHH
Sbjct: 2791 LRDVPAGHH 2817
>gb|CN821029.1|CN821029 HRO4422_G03_M06ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4422_G03_M06, mRNA
sequence
Length = 508
Score = 171 bits (367), Expect = 2e-043
Identities = 68/105 (64%), Positives = 82/105 (78%)
Frame = +2 / +3
Query: 383 YNNLFDAMVDAVVAALEKAGAPNVRVVVSEXGWPSAGGFGASVDNARKYNQGLIDHVGRG 562
Y NLFDA+VD +AL AGA NV++VVSE GWPSAGG AS NA+ YNQ LI+HVG+G
Sbjct: 39 YQNLFDALVDTFYSALGSAGAGNVKIVVSESGWPSAGGTAASAANAQTYNQNLINHVGKG 218
Query: 563 TPKRTGPLETFVFAMFNENQKGGDPTEKNFGLFYGNKQPVYPIRF 697
TPKR G +ET++FAMFNEN+K GD TEK+FGLF +K P Y I+F
Sbjct: 219 TPKRPGAIETYIFAMFNENKKTGDETEKHFGLFNTDKSPAYSIKF 353
Score = 96.8 bits (205), Expect = 4e-021
Identities = 45/70 (64%), Positives = 46/70 (65%)
Frame = -1 / -1
Query: 660 KRPKFFSVGSPPFWFSLNMAKTKVSSGPVLLGVPRPTWSMSPWLYFRALSTLAPKPPAEG 481
K PK FSV SP F FSLNMA VS P LLGVP PTW M WL AL+ LA PPAEG
Sbjct: 316 KSPKCFSVSSPVFLFSLNMANMYVSMAPGLLGVPFPTWLMRFWL*VCALAALAAVPPAEG 137
Query: 480 QPXSDTTTLT 451
P SDTT LT
Sbjct: 136 HPLSDTTILT 107
>emb|Z15131.1|ASBGLUCAN A.sativa mRNA for beta glucanase
Length = 1448
Score = 125 bits (267), Expect(3) = 7e-036
Identities = 52/81 (64%), Positives = 60/81 (74%)
Frame = +2 / +1
Query: 383 YNNLFDAMVDAVVAALEKAGAPNVRVVVSEXGWPSAGGFGASVDNARKYNQGLIDHVGRG 562
Y NLFD VDA A+ K G V++VVSE GWPSAGG A+ NAR YNQ LI+HVGRG
Sbjct: 718 YQNLFDTTVDAFYTAMGKHGGAGVKLVVSESGWPSAGGEAATPANARIYNQYLINHVGRG 897
Query: 563 TPKRTGPLETFVFAMFNENQK 625
TP+ G +ET+VFAMFNENQK
Sbjct: 898 TPRHPGGIETYVFAMFNENQK 960
Score = 34.5 bits (69), Expect(3) = 7e-036
Identities = 13/19 (68%), Positives = 15/19 (78%)
Frame = +2 / +1
Query: 641 EKNFGLFYGNKQPVYPIRF 697
E+N+GLFY N Q VYPI F
Sbjct: 973 EQNWGLFYPNMQHVYPISF 1029
Score = 24.0 bits (46), Expect(3) = 7e-036
Identities = 8/18 (44%), Positives = 10/18 (55%)
Frame = +1 / +1
Query: 259 GXRXSPNVYPYFAYRDNP 312
G N+YPY A+ NP
Sbjct: 595 GSPLMANIYPYLAWAYNP 648
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,161,209
Number of Sequences: 8143
Number of extensions: 82985
Number of successful extensions: 599
Number of sequences better than 1.0e-020: 5
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 307986514
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)