TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2161284.2.6
(621 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN818151.1|CN818151 HRO4471_D03_G05ZS5 Lib AA070E1X Aven... 177 2e-045
gb|CN815851.1|CN815851 HRO4511_B11_D21ZS5 Lib AA071E1X Aven... 176 4e-045
gb|BE439229.1|BE439229 CDO1436_WHE03E06R ITEC CDO Oat cDNA ... 108 1e-024
>gb|CN818151.1|CN818151 HRO4471_D03_G05ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4471_D03_G05, mRNA sequence
Length = 547
Score = 177 bits (380), Expect = 2e-045
Identities = 74/83 (89%), Positives = 81/83 (97%)
Frame = -3 / +3
Query: 487 VTMSRSGQPPDLKKYMDKKLQIKMNANRVVIGTLRGFDQFMNLVVDNTVEVNGNDKTDIG 308
V MSRSGQPPDLKKYMDKKLQIK+NANRVVIGTLRGFDQFMNLVVDNTVEVNG+++ DIG
Sbjct: 72 VAMSRSGQPPDLKKYMDKKLQIKLNANRVVIGTLRGFDQFMNLVVDNTVEVNGDERNDIG 251
Query: 307 MVVIRGNSVVMIEALEPVARSQ* 239
MVV+RGNSVVMIEALEP+A+SQ*
Sbjct: 252 MVVLRGNSVVMIEALEPIAKSQ* 320
Score = 119 bits (255), Expect = 4e-028
Identities = 46/81 (56%), Positives = 61/81 (75%)
Frame = -2 / +2
Query: 482 HEPLGAASGSQEVHGQEASD*DECKPCCYRHTSWVRPVHESGGGQHCGGQWK*QNRYWNG 303
HEP+GAA+GS+EVHGQ+A+D ECKPCC+ H S VR VHE GQHCGGQW+*+ R+ +G
Sbjct: 77 HEPIGAAAGSEEVHGQKATDQAECKPCCHWHASRVRSVHEFSCGQHCGGQWR*KERHRDG 256
Query: 302 GYQGKQCCNDRGTGASCEVTV 240
G + + C+D +CEV+V
Sbjct: 257 GSERE*RCDD*SP*TNCEVSV 319
Score = 119 bits (254), Expect = 5e-028
Identities = 55/83 (66%), Positives = 58/83 (69%)
Frame = +3 / -1
Query: 240 HCDLATGSSASXXXXXXXXXXXXXXSVLSFPLTSTVLSTTRFMNWSNPRSVPITTRFAFI 419
H D A GS AS S LS PLTSTVLSTT+FMN SNPRSVP+TTRFAF
Sbjct: 319 H*DFAIGSRASIITTLFPLRTTIPMSFLSSPLTSTVLSTTKFMN*SNPRSVPMTTRFAFS 140
Query: 420 LI*SFLSMYFLRSGGCPERLMVT 488
LI SFLSMYF RSGGCP+RLM T
Sbjct: 139 LICSFLSMYFFRSGGCPDRLMAT 71
Score = 102 bits (217), Expect = 6e-023
Identities = 43/83 (51%), Positives = 55/83 (66%)
Frame = +2 / -3
Query: 239 SL*PRNWLQCLDHYNTVSPDNHHSNICFVISIDLHSVVHHQIHELVEPTKCADNNTVCIX 418
SL RNW + +H+N + NHH + ISIDLHSVVH+ IHEL+EP+K A++NTVCI
Sbjct: 320 SLRLRNWFKGFNHHNAIPSQNHHPYVVPFISIDLHSVVHN*IHELIEPSKRANDNTVCIQ 141
Query: 419 XXXXXXXXXXXEIRRLPRAAHGD 487
+IRRLPR+AHGD
Sbjct: 140 LDL*LFVHVLLQIRRLPRSAHGD 72
>gb|CN815851.1|CN815851 HRO4511_B11_D21ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4511_B11_D21, mRNA sequence
Length = 419
Score = 176 bits (378), Expect = 4e-045
Identities = 74/80 (92%), Positives = 79/80 (98%)
Frame = -3 / +3
Query: 481 MSRSGQPPDLKKYMDKKLQIKMNANRVVIGTLRGFDQFMNLVVDNTVEVNGNDKTDIGMV 302
MSRSGQPPDLKKYMDKKLQIKMNANRVVIGTLRGFDQFM LVVDNTVEVNG++KTDIGMV
Sbjct: 36 MSRSGQPPDLKKYMDKKLQIKMNANRVVIGTLRGFDQFMILVVDNTVEVNGDEKTDIGMV 215
Query: 301 VIRGNSVVMIEALEPVARSQ 242
V+RGNSVVMIEALEP+A+SQ
Sbjct: 216 VVRGNSVVMIEALEPIAKSQ 275
Score = 142 bits (305), Expect = 5e-035
Identities = 53/81 (65%), Positives = 64/81 (79%)
Frame = -2 / +2
Query: 488 RHHEPLGAASGSQEVHGQEASD*DECKPCCYRHTSWVRPVHESGGGQHCGGQWK*QNRYW 309
R HEP+ ASGSQEVHGQEA+D DECKPCCY H + + V++SGGGQH GGQW *++ YW
Sbjct: 29 RRHEPIWPASGSQEVHGQEATDQDECKPCCYWHPTRI*SVYDSGGGQHRGGQWG*ED*YW 208
Query: 308 NGGYQGKQCCNDRGTGASCEV 246
NGG QGKQ C+D GTG C++
Sbjct: 209 NGGCQGKQRCHD*GTGTDCQI 271
Score = 119 bits (255), Expect(2) = 3e-029
Identities = 56/80 (70%), Positives = 58/80 (72%)
Frame = +3 / -2
Query: 243 CDLATGSSASXXXXXXXXXXXXXXSVLSFPLTSTVLSTTRFMNWSNPRSVPITTRFAFIL 422
CDLA GSSAS SV S PLTSTVLSTTR +N SNPR VPITTRFAFIL
Sbjct: 274 CDLAIGSSASIMTTLFPLTTTIPISVFSSPLTSTVLSTTRIIN*SNPRRVPITTRFAFIL 95
Query: 423 I*SFLSMYFLRSGGCPERLM 482
I SFLSMYFLRSGG P+RLM
Sbjct: 94 ICSFLSMYFLRSGGWPDRLM 35
Score = 101 bits (215), Expect = 1e-022
Identities = 43/76 (56%), Positives = 51/76 (67%)
Frame = +2 / -1
Query: 254 NWLQCLDHYNTVSPDNHHSNICFVISIDLHSVVHHQIHELVEPTKCADNNTVCIXXXXXX 433
N QCL+H N VSPDNHHSNI +I IDLH VVHHQ H+L++ + A+NNTVCI
Sbjct: 263 NRFQCLNHDNAVSPDNHHSNISLLIPIDLHGVVHHQNHKLIKSS*GANNNTVCIHLDL*L 84
Query: 434 XXXXXXEIRRLPRAAH 481
EIRRL R+AH
Sbjct: 83 LVHVLLEIRRLARSAH 36
Score = 23.1 bits (44), Expect(2) = 3e-029
Identities = 9/17 (52%), Positives = 10/17 (58%)
Frame = +3 / -2
Query: 159 KALVKYNRVH*YLLHQF 209
K LVK H Y +HQF
Sbjct: 379 KTLVKIQHEHEYHIHQF 329
>gb|BE439229.1|BE439229 CDO1436_WHE03E06R ITEC CDO Oat cDNA Library Avena sativa cDNA clone
CDO1436_WHE03E06, mRNA sequence
Length = 234
Score = 108 bits (230), Expect = 1e-024
Identities = 44/49 (89%), Positives = 48/49 (97%)
Frame = -3 / +1
Query: 454 LKKYMDKKLQIKMNANRVVIGTLRGFDQFMNLVVDNTVEVNGNDKTDIG 308
LKKYMDKKLQIK+NANRVVIGTLRGFDQFMNLVVDNTVEVNG+++ DIG
Sbjct: 88 LKKYMDKKLQIKLNANRVVIGTLRGFDQFMNLVVDNTVEVNGDERNDIG 234
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,755,182
Number of Sequences: 8143
Number of extensions: 97147
Number of successful extensions: 1147
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 200978884
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)