TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1805320.2.1
(955 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815277.1|CN815277 HRO4507_H06_P11ZS5 Lib AA071E1X Aven... 142 1e-040
>gb|CN815277.1|CN815277 HRO4507_H06_P11ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4507_H06_P11, mRNA sequence
Length = 458
Score = 142 bits (305), Expect(2) = 1e-040
Identities = 59/60 (98%), Positives = 60/60 (100%)
Frame = -2 / +1
Query: 579 EDLKNWDADFVKVDQATLFDLILAANYLNIKGLLDLTCQTVADMIKGKTPEEIRKTFNIK 400
EDLKNWDA+FVKVDQATLFDLILAANYLNIKGLLDLTCQTVADMIKGKTPEEIRKTFNIK
Sbjct: 40 EDLKNWDAEFVKVDQATLFDLILAANYLNIKGLLDLTCQTVADMIKGKTPEEIRKTFNIK 219
Score = 110 bits (235), Expect(2) = 2e-029
Identities = 42/60 (70%), Positives = 48/60 (80%)
Frame = -1 / +3
Query: 577 GPQELGCGLRQGRPGHAFRPHLGCKLSEHQGSARPDLSDCC*HDQGQDTRRDSQDLQHQE 398
GPQELG +RQGRPGH RPH GC+L +HQG+A P + DCC*HDQGQD R D QDLQHQ+
Sbjct: 42 GPQELGR*VRQGRPGHPIRPHPGCQLPQHQGTAGPYVPDCC*HDQGQDPRGDPQDLQHQD 221
Score = 103 bits (220), Expect(2) = 8e-032
Identities = 49/58 (84%), Positives = 50/58 (86%)
Frame = +2 / -1
Query: 401 LMLKVLRISSGVLPLIMSATV*QVRSSRPLMFR*FAAKMRSKSVAWSTLTKSASQFLR 574
LMLKVLRISSGVLPLIMSATV VRSS PLM R* AA+MRS VAWSTLT SASQFLR
Sbjct: 218 LMLKVLRISSGVLPLIMSATVWHVRSSSPLMLR*LAARMRSNRVAWSTLTNSASQFLR 45
Score = 91.3 bits (193), Expect(2) = 1e-026
Identities = 39/55 (70%), Positives = 47/55 (85%)
Frame = +1 / -3
Query: 400 LDVEGLANLFWCLALDHVSNSLTGQVEQTLDVQIVCSQDEVEKRGLVDLDEVRIP 564
LDVEGLA+L W LALDHVSNSL +V+Q+LDV++V SQDEVE GLVDLDE+ +P
Sbjct: 219 LDVEGLADLLWGLALDHVSNSLARKVQQSLDVEVVGSQDEVE*GGLVDLDELSVP 55
Score = 48.7 bits (100), Expect(2) = 8e-032
Identities = 19/22 (86%), Positives = 21/22 (95%)
Frame = +2 / -3
Query: 338 YSKAHWFSLRISSSSSGVKSFL 403
YSKAHWFS+RISSSSS VKS+L
Sbjct: 282 YSKAHWFSMRISSSSSAVKSYL 217
Score = 43.7 bits (89), Expect(2) = 1e-026
Identities = 17/20 (85%), Positives = 18/20 (90%)
Frame = +3 / -1
Query: 336 LTRRPTGSPCGSPLPPQG*S 395
+TRR TGSPCGSPLPPQ *S
Sbjct: 284 ITRRHTGSPCGSPLPPQP*S 225
Score = 39.1 bits (79), Expect(2) = 1e-040
Identities = 16/19 (84%), Positives = 16/19 (84%)
Frame = -1 / +1
Query: 394 LHP*GGRGDPQGEPVGLRV 338
LH *GGRGDP GEPV LRV
Sbjct: 226 LHG*GGRGDPHGEPVCLRV 282
Score = 34.1 bits (68), Expect(2) = 2e-029
Identities = 16/19 (84%), Positives = 16/19 (84%)
Frame = -3 / +3
Query: 395 TSPLRRKRRSAGRTSGPSS 339
TS LRRKRRSA RTS PSS
Sbjct: 225 TSRLRRKRRSAWRTSVPSS 281
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,520,768
Number of Sequences: 8143
Number of extensions: 106509
Number of successful extensions: 632
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 333550612
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)