TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1805016.2.1
(1645 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN180781.1|CN180781 As03_isu1961_C ISU cDNA from oat Ave... 358 e-113
>gb|CN180781.1|CN180781 As03_isu1961_C ISU cDNA from oat Avena sativa cDNA clone
As03_isu1961, mRNA sequence
Length = 1004
Score = 358 bits (775), Expect(2) = e-113
Identities = 144/214 (67%), Positives = 169/214 (78%)
Frame = -3 / -1
Query: 1211 DLYNGTTKKLSLSRSALXXXXXXXXXXXXXXGTCHGCRGAGMRTITRQIGLGMIQQMNTV 1032
+LYNGT+KKLSLSR+ L C GC+GAG + RQ+G GMIQQM
Sbjct: 1004 ELYNGTSKKLSLSRNVLCSKCNGKGSKSGASMKCAGCQGAGYKVQIRQLGPGMIQQMQQP 825
Query: 1031 CPECKGSGEIISDKDKCPSCKGNKVVQEKKVLEVHVEKGMQHNQKIVFQGQADEAPDTVT 852
C EC+G+GE ISDKD+C CKG KVVQEKKVLEV VEKGMQH+QKI F G+ADEAPDTVT
Sbjct: 824 CNECRGTGETISDKDRCGQCKGEKVVQEKKVLEVVVEKGMQHSQKITFPGEADEAPDTVT 645
Query: 851 GDIVFVLQLKDHPKFKRMYDDLYVEHTISLTEALCGFQFVLTHLDGRQLLIKSDPGEVIK 672
GDI+FVLQ K+HPKFKR DDL+ EHT++LTEALCGFQ+VLTHLDGRQLLIKS+PGEV+K
Sbjct: 644 GDIIFVLQQKEHPKFKRKGDDLFYEHTLTLTEALCGFQYVLTHLDGRQLLIKSNPGEVVK 465
Query: 671 PGQHKAINDEGMPQHGRPFMKGRLFVEFNVEFPE 570
P KAINDEGMP + RPFMKG+L++ F V+FP+
Sbjct: 464 PDSFKAINDEGMPMYQRPFMKGKLYIHFTVDFPD 363
Score = 245 bits (530), Expect(3) = 5e-068
Identities = 116/212 (54%), Positives = 131/212 (61%)
Frame = +1 / +1
Query: 571 SGNSTLNSTNRRPFMKGRPC*GIPSSLMALC*PGLITSPGSDLIRSCRPSR*VRTNWKPH 750
SG ST+ PFM G G+PSSL+AL GL TSPG DL+ SC PS+*VRT WKPH
Sbjct: 364 SGKSTVKWM*SLPFMNGLWYMGMPSSLIALNESGLTTSPGLDLMSSCLPSK*VRTYWKPH 543
Query: 751 NASVREIVCSTYKSSYILLNFGWSLSCKTKTMSPVTVSGASSA*PWNTIFWLCCIPXXXX 930
ASVR VCS +SS LN G S C+TK MSPVTVSGASSA P IFWLCCIP
Sbjct: 544 RASVRVRVCS*KRSSPFRLNLGCSFCCRTKIMSPVTVSGASSASPGKVIFWLCCIPFSTT 723
Query: 931 XXXXXXXXXXLFPLQLGHLSLSLMISPDPLHSGQTVFICWIMPRPICLVIVLIPAPRQPW 1110
PL HLSLSLM+SP PLHS Q IC I+P P + L PAP QP
Sbjct: 724 TSRTFFSCTTFSPLHCPHLSLSLMVSPVPLHSLQGCCIC*IIPGPNWRIWTL*PAPWQPA 903
Query: 1111 HVPDAPLLDPFPLHLEHKALRDKDSFLVVPLY 1206
H +AP +P PLH E LR+++SFL VPLY
Sbjct: 904 HFIEAPDFEPLPLHFEQSTLRERESFLDVPLY 999
Score = 68.9 bits (144), Expect(2) = e-113
Identities = 25/42 (59%), Positives = 34/42 (80%)
Frame = -3 / -1
Query: 569 PGVLSPAQCRSLEKILPPKPGSQLSDMELDQCEETTLHDVNI 444
P L+P QC++LE +LPPKP SQ +DMELD+CEET +D++I
Sbjct: 368 PDSLNPDQCKALETVLPPKPVSQYTDMELDECEETMAYDIDI 243
Score = 27.2 bits (53), Expect(3) = 5e-068
Identities = 10/20 (50%), Positives = 11/20 (55%)
Frame = +1 / +1
Query: 511 GFGGRIFSNERHWAGESTPG 570
G GGR S HW+G S G
Sbjct: 310 GLGGRTVSRALHWSGFSESG 369
Score = 27.2 bits (53), Expect(3) = 5e-068
Identities = 7/7 (100%), Positives = 7/7 (100%)
Frame = +1 / +1
Query: 343 YCWAHCT 363
YCWAHCT
Sbjct: 136 YCWAHCT 156
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 19,514,333
Number of Sequences: 8143
Number of extensions: 293133
Number of successful extensions: 1364
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 45
effective length of database: 1,201,052
effective search space used: 602928104
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)