TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1804944.2.2
(4065 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815813.1|CN815813 HRO4510_G06_M12ZS5 Lib AA071E1X Aven... 197 1e-050
gb|CN819884.1|CN819884 HRO4407_C09_F17ZS5 Lib AA069E1X Aven... 187 1e-047
gb|CN819594.1|CN819594 HRO4401_H12_O23ZS5 Lib AA069E1X Aven... 164 8e-041
gb|CN815647.1|CN815647 HRO4514_A01_A02ZS5 Lib AA071E1X Aven... 135 4e-032
>gb|CN815813.1|CN815813 HRO4510_G06_M12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4510_G06_M12, mRNA sequence
Length = 521
Score = 197 bits (425), Expect = 1e-050
Identities = 73/107 (68%), Positives = 89/107 (83%)
Frame = -2 / +2
Query: 986 KWXXXXXXXXXXXXLNFIGVVAGVSNAINNGYESWGPLFGKLFFAFWVIVHLYPFLKGLV 807
+W +N +G+VAGVS+A+NNGY SWGPLFGKLFF+FWVIVHLYPFLKGL+
Sbjct: 11 QWTTLLIPPTTLIIINMVGIVAGVSDAVNNGYGSWGPLFGKLFFSFWVIVHLYPFLKGLM 190
Query: 806 GRQNRTPTIVIVWSILLASIFSLLWVRIDPFLAKDDGPLLEECGLDC 666
GRQNRTPTIV++WS+LLASIFSL+WVRIDPF+AK GP+L+ CG+ C
Sbjct: 191 GRQNRTPTIVVLWSVLLASIFSLVWVRIDPFIAKSKGPILKPCGVQC 331
Score = 141 bits (303), Expect = 7e-034
Identities = 61/92 (66%), Positives = 67/92 (72%)
Frame = +1 / -3
Query: 709 ARKGSIRTQRSEKIEASRMDQTMTIVGVLFCLPTKPLRKG*RWTITQNAKNSFPKRGPHD 888
A KGSI TQ EK+EASR DQ+ T+VGV FCLP PLR G*RWT+TQ K SFPKRGPHD
Sbjct: 288 AMKGSILTQTREKMEASRTDQSTTMVGVRFCLPMSPLRNG*RWTMTQKEKKSFPKRGPHD 109
Query: 889 SYPLLIAFETPATTPMKFNRSKVVGGINKVVH 984
PLL A ETPAT P VVGGI++VVH
Sbjct: 108 P*PLLTASETPATMPTMLMMMSVVGGISRVVH 13
>gb|CN819884.1|CN819884 HRO4407_C09_F17ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4407_C09_F17, mRNA
sequence
Length = 676
Score = 187 bits (402), Expect = 1e-047
Identities = 72/92 (78%), Positives = 82/92 (89%)
Frame = -2 / +1
Query: 941 NFIGVVAGVSNAINNGYESWGPLFGKLFFAFWVIVHLYPFLKGLVGRQNRTPTIVIVWSI 762
N +GVVAG S AIN+GY+SWGPLFGKLFFAFWVIVHLYPFLKGL+GR NRTPTIVIVW+I
Sbjct: 49 NMVGVVAGTSYAINSGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRTNRTPTIVIVWAI 228
Query: 761 LLASIFSLLWVRIDPFLAKDDGPLLEECGLDC 666
LLASIFSLLWVRIDPF + GP ++ CG++C
Sbjct: 229 LLASIFSLLWVRIDPFTTRVAGPNIQTCGINC 324
Score = 115 bits (246), Expect = 5e-026
Identities = 54/87 (62%), Positives = 59/87 (67%)
Frame = +1 / -3
Query: 718 GSIRTQRSEKIEASRMDQTMTIVGVLFCLPTKPLRKG*RWTITQNAKNSFPKRGPHDSYP 897
GSIRT EKIEA R+ QTMTIVGV F LP +PLR G + TITQ AK SFP GPHD P
Sbjct: 272 GSIRTHSKEKIEARRIAQTMTIVGVRFVLPIRPLRNGYK*TITQKAKKSFPNSGPHDW*P 93
Query: 898 LLIAFETPATTPMKFNRSKVVGGINKV 978
LL+A E PATTP VVGG+ V
Sbjct: 92 LLMA*EVPATTPTMLMIKIVVGGMRSV 12
>gb|CN819594.1|CN819594 HRO4401_H12_O23ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4401_H12_O23, mRNA
sequence
Length = 620
Score = 164 bits (353), Expect = 8e-041
Identities = 62/78 (79%), Positives = 71/78 (91%)
Frame = -2 / +2
Query: 899 NGYESWGPLFGKLFFAFWVIVHLYPFLKGLVGRQNRTPTIVIVWSILLASIFSLLWVRID 720
+GY+SWGPLFGKLFFAFWVIVHLYPFLKGL+GR NRTPTIVIVW+ILLASIFSLLWVRID
Sbjct: 2 SGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRTNRTPTIVIVWAILLASIFSLLWVRID 181
Query: 719 PFLAKDDGPLLEECGLDC 666
PF + GP ++ CG++C
Sbjct: 182 PFTTRVAGPNIQTCGINC 235
>gb|CN815647.1|CN815647 HRO4514_A01_A02ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4514_A01_A02, mRNA sequence
Length = 474
Score = 135 bits (290), Expect = 4e-032
Identities = 54/60 (90%), Positives = 57/60 (95%)
Frame = -2 / +3
Query: 845 VIVHLYPFLKGLVGRQNRTPTIVIVWSILLASIFSLLWVRIDPFLAKDDGPLLEECGLDC 666
VIVHLYPFLKGL+GRQNRTPTIVIVWSILLASI SLLWVR++PFLAK DGP LEECGLDC
Sbjct: 12 VIVHLYPFLKGLLGRQNRTPTIVIVWSILLASIISLLWVRVNPFLAKTDGPTLEECGLDC 191
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 47,445,745
Number of Sequences: 8143
Number of extensions: 741881
Number of successful extensions: 3408
Number of sequences better than 1.0e-020: 4
length of database: 1,567,487
effective HSP length: 47
effective length of database: 1,184,766
effective search space used: 1548489162
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)