TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1804904.2.3
(801 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN819453.1|CN819453 HRO4406_A10_A20ZS5 Lib AA069E1X Aven... 266 3e-072
>gb|CN819453.1|CN819453 HRO4406_A10_A20ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4406_A10_A20, mRNA
sequence
Length = 567
Score = 266 bits (576), Expect = 3e-072
Identities = 121/155 (78%), Positives = 130/155 (83%)
Frame = +2 / +1
Query: 155 YGRLVVIVDVVDQNRALVDAPDMVRCQINFKRLSLTDIKIDIKRVPKKSTLIKAMEEADV 334
YGRLVVIVDVVDQNRALVDAPDMVRCQINFKRLSLTDIKIDIKRVPKK+TLIKAMEEADV
Sbjct: 1 YGRLVVIVDVVDQNRALVDAPDMVRCQINFKRLSLTDIKIDIKRVPKKATLIKAMEEADV 180
Query: 335 KTKWENSSWGKKLIVQKRRAALSDFDRFKVMLARIKRGGAIRQELSKLKKASTA*VPFGG 514
K+KWENSSWGKKLIVQKRRA+L+DFDRFKVMLA+IKRGGAIRQEL+KLKK A* FGG
Sbjct: 181 KSKWENSSWGKKLIVQKRRASLNDFDRFKVMLAKIKRGGAIRQELAKLKKEVAA*NIFGG 360
Query: 515 *HVSFWFHVGSAICFECCQSQN**YVSCVPVSNFI 619
*HV F+ F C S N* Y P+S +
Sbjct: 361 *HVEFFVPSWICTMF*CP*SHN*YYCFQEPISYIV 465
Score = 204 bits (439), Expect = 2e-053
Identities = 96/152 (63%), Positives = 104/152 (68%)
Frame = -1 / -2
Query: 612 FETGTQETYY*FWL*QHSKQIADPT*NQKLTCHPPKGT*AVDAFFSLESSCLIAPPLLIL 433
+E G+ + Y* WL H + + TCHPPK *A +F SL SSCLIAPPLLIL
Sbjct: 458 YEIGS*KQ*Y*LWLQGH*NIVQIQLGTKNSTCHPPKIF*AATSFLSLASSCLIAPPLLIL 279
Query: 432 ASMTXXXXXXXXXXXLFWTISFLPHELFSHLVFTSASSIALIKVDFLGTRLMSILMSVSE 253
A+MT LFWTISFLPHELFSHL+FTSASSIALIKV FLGTRLMSILMSV E
Sbjct: 278 ANMTLNLSKSFSDALLFWTISFLPHELFSHLLFTSASSIALIKVAFLGTRLMSILMSVRE 99
Query: 252 SRLKLIWHLTISGASTSALFWXXXXXXXXXRP 157
RLKLIWHLTISGASTSA FW RP
Sbjct: 98 RRLKLIWHLTISGASTSARFWSTTSTMTTRRP 3
Score = 171 bits (367), Expect = 2e-043
Identities = 81/136 (59%), Positives = 90/136 (66%)
Frame = +1 / +3
Query: 202 TCGRP*YGQVPD*LQTALTYRHQD*HQTCPQEVNLDQGYGGS*CED*VGEQLMGQEADCP 381
TCG YGQVPD*LQ + + H D HQ CPQE N DQG GGS*CE+ VGEQL+GQEA CP
Sbjct: 48 TCGCSRYGQVPD*LQASFSD*H*DRHQACPQEGNFDQGNGGS*CEEQVGEQLVGQEAYCP 227
Query: 382 EEEGCTQ*L*QVQGHAGKDQEGWCYQARALQAEEGVHCLSPFWWMTC*FLVSCWISNLF* 561
EE+ +*L*QVQGH G D E CYQARA QA+EG CL WWMTC* S +
Sbjct: 228 EEKSVAE*L*QVQGHVG*D*ERRCYQARACQAQEGGCCLEYLWWMTC*VFCSKLDLHYVL 407
Query: 562 VLSKPELVICFLCTSF 609
V KP LV+ F T F
Sbjct: 408 VSLKP*LVLLFSGTYF 455
Score = 152 bits (326), Expect = 8e-038
Identities = 70/117 (59%), Positives = 81/117 (69%)
Frame = -3 / -1
Query: 529 KTNMSSTKRDLSSGRLLQLGELLPDSTTPLDPCQHDLEPVKVTECSPPLLDNQXXXXXXX 350
K NMSSTK LSS LL+LG+LLPDST L+ QHDLEPVKV + LLDN+
Sbjct: 375 KLNMSSTKDILSSNLLLELGKLLPDSTASLNLSQHDLEPVKVIQRRSSLLDNKLLAPRAV 196
Query: 349 XXXXLHISFLHSLDQG*LLGDTFDVNLDVGK*EPFEVNLAPDHIRGVHKCPVLVDHI 179
LHISFLH LDQ LLGDT DV+L+V + + EVNLAPDHI +HKCPVLVD +
Sbjct: 195 LPLALHISFLHCLDQSCLLGDTLDVDLNVSQRKTLEVNLAPDHIGSIHKCPVLVDDV 25
Score = 138 bits (295), Expect = 1e-033
Identities = 74/138 (53%), Positives = 84/138 (60%)
Frame = -2 / -3
Query: 611 LKLVHRKHITNSGFDNTQNKLLIQHETKN*HVIHQKGLKQWTPSSAWRALA**HHPS*SL 432
+K V TN GF +T+ E K HVIHQ+ KQ PS AW+ALA**H S*S
Sbjct: 457 MK*VPENSSTNYGFKDTRT*CRSNLEQKTQHVIHQRYSKQQPPS*AWQALA**HRLS*S* 278
Query: 431 PA*P*TCQSH*VQPSSSGQXXXXXXXXXXXXXSHQLPP*P*SRLTSWGHV*CQS*CR*VR 252
P *P*TCQSH SSGQ SHQLPP P*S+L SWGH *C+S*C+ +
Sbjct: 277 PT*P*TCQSHSATLFSSGQ*ASCPTSCSPTCSSHQLPPLP*SKLPSWGHA*CRS*CQSEK 98
Query: 251 AV*S*SGT*PYQGRPQVP 198
*S*SGT*PY+ PQVP
Sbjct: 97 DA*S*SGT*PYREHPQVP 44
Score = 125 bits (268), Expect = 8e-030
Identities = 68/129 (52%), Positives = 74/129 (57%)
Frame = +3 / +2
Query: 198 GHLWTPLIWSGARLTSNGSHLPTSRLTSNVSPRSQP*SRLWRKLM*RLSGRTAHGARS*L 377
GHLW IWSGARLTS+ T R TS+VSPR Q *SR WRKLM*R SGRTA GARS L
Sbjct: 44 GHLWMLPIWSGARLTSSVFL*LTLRSTSSVSPRRQL*SRQWRKLM*RASGRTARGARSLL 223
Query: 378 SRRGGLHSVTLTGSRSCWQGSRGVVXXXXXXXXXXXXXXXXXXXVDDMLVFGFMLDQQFV 557
SRR +TLTGSRSCW R V VDDML F F +
Sbjct: 224 SRREERR*MTLTGSRSCWLRLREAVLSGKSLPSSRRRLLLRISLVDDMLSFLFQVGSALC 403
Query: 558 LSVVKARIS 584
SV++A IS
Sbjct: 404 SSVLEAIIS 430
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,756,959
Number of Sequences: 8143
Number of extensions: 123901
Number of successful extensions: 724
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 271466374
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)