TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1804904.2.1
(690 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN819453.1|CN819453 HRO4406_A10_A20ZS5 Lib AA069E1X Aven... 238 8e-064
>gb|CN819453.1|CN819453 HRO4406_A10_A20ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4406_A10_A20, mRNA
sequence
Length = 567
Score = 238 bits (514), Expect = 8e-064
Identities = 102/104 (98%), Positives = 103/104 (99%)
Frame = +1 / +1
Query: 130 YGRLVVIVDVVDQNRALVDAPDMVRCQMNFKRLSLTDIKIDIKRVPKKTTLIKAMEEADV 309
YGRLVVIVDVVDQNRALVDAPDMVRCQ+NFKRLSLTDIKIDIKRVPKK TLIKAMEEADV
Sbjct: 1 YGRLVVIVDVVDQNRALVDAPDMVRCQINFKRLSLTDIKIDIKRVPKKATLIKAMEEADV 180
Query: 310 KSKWENSSWGKKLIVQKRRASLNDFDRFKVMLAKIKRGGAIRQE 441
KSKWENSSWGKKLIVQKRRASLNDFDRFKVMLAKIKRGGAIRQE
Sbjct: 181 KSKWENSSWGKKLIVQKRRASLNDFDRFKVMLAKIKRGGAIRQE 312
Score = 193 bits (416), Expect = 3e-050
Identities = 86/100 (86%), Positives = 88/100 (88%)
Frame = -2 / -2
Query: 431 MAPPLLIFANMTLNLSKSLSDALLF*TISFLPHELFSHLLFTSASSIALISVVFFGTRLM 252
+APPLLI ANMTLNLSKS SDALLF TISFLPHELFSHLLFTSASSIALI V F GTRLM
Sbjct: 302 IAPPLLILANMTLNLSKSFSDALLFWTISFLPHELFSHLLFTSASSIALIKVAFLGTRLM 123
Query: 251 SILMSVRESRLKFIWQRTMSGASTRALFWSTTSTMTTRRP 132
SILMSVRE RLK IW T+SGAST A FWSTTSTMTTRRP
Sbjct: 122 SILMSVRERRLKLIWHLTISGASTSARFWSTTSTMTTRRP 3
Score = 137 bits (293), Expect = 2e-033
Identities = 59/91 (64%), Positives = 71/91 (78%)
Frame = +3 / +3
Query: 168 EQGSCGCPRHGSLPNELQAAFPD*HQD*H*TCPKEDYTDQGNGGS*CEKQVGE*LMGQEA 347
E G+CGC R+G +P+ LQA+F D*H D H CP+E DQGNGGS*CE+QVGE L+GQEA
Sbjct: 39 EPGTCGCSRYGQVPD*LQASFSD*H*DRHQACPQEGNFDQGNGGS*CEEQVGEQLVGQEA 218
Query: 348 DCSEEESITQ*L*QVQGHVGEDQEGWCHQAR 440
C EE+S+ +*L*QVQGHVG D E C+QAR
Sbjct: 219 YCPEEKSVAE*L*QVQGHVG*D*ERRCYQAR 311
Score = 114 bits (243), Expect = 2e-026
Identities = 58/88 (65%), Positives = 61/88 (69%)
Frame = +2 / +2
Query: 173 GLLWMPPTWFVAK*TSSGFP*LTSRLTLNVSQRRLH*SRQWRKLM*KASGRIAHGARS*L 352
G LWM P W A+ TSS F *LT R T +VS RR *SRQWRKLM*+ASGR A GARS L
Sbjct: 44 GHLWMLPIWSGARLTSSVFL*LTLRSTSSVSPRRQL*SRQWRKLM*RASGRTARGARSLL 223
Query: 353 FRRGEHHSMTLTGSRSCWRRSRGVVPSG 436
RR E MTLTGSRSCW R R V SG
Sbjct: 224 SRREERR*MTLTGSRSCWLRLREAVLSG 307
Score = 76.7 bits (161), Expect(3) = 2e-030
Identities = 31/46 (67%), Positives = 37/46 (80%)
Frame = -1 / -1
Query: 309 HISFLHCLDQCSLLWDTFNVNLDVSQGKPLEVHLATNHVGGIHKSP 172
HISFLHCLDQ LL DT +V+L+VSQ K LEV+LA +H+G IHK P
Sbjct: 180 HISFLHCLDQSCLLGDTLDVDLNVSQRKTLEVNLAPDHIGSIHKCP 43
Score = 49.2 bits (101), Expect(3) = 2e-030
Identities = 22/31 (70%), Positives = 22/31 (70%)
Frame = -3 / -3
Query: 394 *TCQSH*VMLSSSEQSASCPMSYSPTCFSHQ 302
*TCQSH L SS Q ASCP S SPTC SHQ
Sbjct: 265 *TCQSHSATLFSSGQ*ASCPTSCSPTCSSHQ 173
Score = 39.1 bits (79), Expect(3) = 2e-030
Identities = 15/21 (71%), Positives = 17/21 (80%)
Frame = -1 / -1
Query: 435 PDGTTPLDLRQHDLEPVKVIE 373
PD T L+L QHDLEPVKVI+
Sbjct: 306 PDSTASLNLSQHDLEPVKVIQ 244
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,730,359
Number of Sequences: 8143
Number of extensions: 77265
Number of successful extensions: 860
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 229164947
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)