TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1738959.2.3
(725 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815139.1|CN815139 HRO4506_C09_E18ZS5 Lib AA071E1X Aven... 181 2e-054
>gb|CN815139.1|CN815139 HRO4506_C09_E18ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4506_C09_E18, mRNA sequence
Length = 476
Score = 181 bits (390), Expect(3) = 2e-054
Identities = 73/80 (91%), Positives = 78/80 (97%)
Frame = +1 / +3
Query: 187 INLGNPGEFTMLELAENVKELINPDVTVTMTENTPDDPRQRKPDITKAKEVLGWEPKIVL 366
IN+GNPGEFTMLELAENVKELINP+VTVTMTENTPDDPRQRKPDI+KAKE+L WEPK+VL
Sbjct: 3 INIGNPGEFTMLELAENVKELINPEVTVTMTENTPDDPRQRKPDISKAKELLDWEPKVVL 182
Query: 367 RDGLVLMEDDFRERLTVPKK 426
RDGLVLMEDDFRERL VPKK
Sbjct: 183 RDGLVLMEDDFRERLAVPKK 242
Score = 159 bits (341), Expect(3) = 8e-046
Identities = 64/82 (78%), Positives = 67/82 (81%)
Frame = -3 / -1
Query: 432 LGFLGHGQALPEIILHEHQAVPQDDLGLPSQNFLRLCDVRLPLAGVIRSVLGHRHCHIWV 253
LG LGH QAL EI+ HEHQAV QDDLGLP Q L L DV LPLA VIRSVL HRHCH+WV
Sbjct: 248 LGLLGHCQALSEIVFHEHQAVAQDDLGLPVQKLLGLADVGLPLARVIRSVLSHRHCHLWV 69
Query: 252 DQLLHILSKFQHGEFTWVPQVN 187
DQLLHILS+FQHGE TWVP VN
Sbjct: 68 DQLLHILSEFQHGELTWVPNVN 3
Score = 144 bits (310), Expect(2) = 2e-038
Identities = 63/82 (76%), Positives = 67/82 (81%)
Frame = +2 / +1
Query: 188 LTWGTQVNSPCWNLLRM*RS*STQM*Q*R*PRTLLMTPARGSRTSQRRRKFWDGSPRSSC 367
LT GTQV+SPCWN LRM*RS*STQ *Q*R* RTLLMT ARGS TS R R FW GSPRSSC
Sbjct: 4 LTLGTQVSSPCWNSLRM*RS*STQR*Q*R*LRTLLMTLARGSPTSARPRSFWTGSPRSSC 183
Query: 368 GTAWCSWRMISGSA*PCPRKPR 433
TAWCSW+ IS SA CPR+P+
Sbjct: 184 ATAWCSWKTISESAWQCPRRPK 249
Score = 131 bits (280), Expect = 2e-031
Identities = 56/76 (73%), Positives = 61/76 (80%)
Frame = +3 / +2
Query: 198 EPR*IHHAGTC*ECEGVDQPRCDSDDDREHS**PPPEEAGHHKGEGSSGMGAQDRPAGRL 377
EPR*+HHAGT *ECEG DQPR DSDDD EHS**P PEEA H +G+G+SG+GAQ RPA RL
Sbjct: 14 EPR*VHHAGTR*ECEGADQPRGDSDDD*EHS**PSPEEARHQQGQGASGLGAQGRPARRL 193
Query: 378 GAHGG*FPGAPDRAQE 425
GAHG F AP AQE
Sbjct: 194 GAHGRRFQRAPGSAQE 241
Score = 116 bits (248), Expect(2) = 2e-029
Identities = 53/80 (66%), Positives = 57/80 (71%)
Frame = -1 / -2
Query: 425 FLGTVXXXXXXXXXXXXXXXXTILGSHPRTSFAFVMSGFLWRGSSGVFSVIVTVTSGLIN 246
FLGT T LGS R+S A +MSGFLWRGSSGVFSVIVTVTSGLI+
Sbjct: 241 FLGTARRSLKSSSMSTKPSRRTTLGSQSRSSLALLMSGFLWRGSSGVFSVIVTVTSGLIS 62
Query: 245 SFTFSASSSMVNSPGFPKLI 186
SFTFSASSSMVNSPGFP L+
Sbjct: 61 SFTFSASSSMVNSPGFPMLM 2
Score = 40.5 bits (82), Expect(3) = 2e-054
Identities = 16/22 (72%), Positives = 17/22 (77%)
Frame = +2 / +1
Query: 560 SFTLKDCIIRIFIQGWPFESLP 625
SF LKDCIIR FI+GW F LP
Sbjct: 379 SFNLKDCIIRHFIRGWLFVVLP 444
Score = 35.9 bits (72), Expect(3) = 8e-046
Identities = 14/23 (60%), Positives = 18/23 (78%)
Frame = -3 / -1
Query: 624 GRDSNGQPCMKILIIQSFKVNDK 556
G +N QP MK L+IQSFK+ND+
Sbjct: 443 GSTTNSQPRMKCLMIQSFKLNDE 375
Score = 29.5 bits (58), Expect(2) = 2e-038
Identities = 12/21 (57%), Positives = 15/21 (71%)
Frame = +1 / +3
Query: 550 YSFVVHLEGLYY*NFHTRLAV 612
+ F+V LE LY+* HT LAV
Sbjct: 369 FVFIVQLERLYH*TLHTGLAV 431
Score = 27.2 bits (53), Expect(2) = 2e-029
Identities = 14/42 (33%), Positives = 22/42 (52%)
Frame = -2 / -3
Query: 649 TQVPQDLRRQRLERPALYENSNNTILQGERQRNIKFIGTQIP 524
+QVPQ+ + + P N ++ ++R FIGTQIP
Sbjct: 462 SQVPQERKYHKQPAPYEVSNDTIFQVER*KRRYNWFIGTQIP 337
Score = 24.4 bits (47), Expect(3) = 2e-054
Identities = 10/14 (71%), Positives = 12/14 (85%)
Frame = +1 / +3
Query: 520 ITEFEFQ*T*YSFV 561
++EFEFQ*T Y FV
Sbjct: 333 LSEFEFQ*TNYIFV 374
Score = 22.1 bits (42), Expect(3) = 8e-046
Identities = 7/7 (100%), Positives = 7/7 (100%)
Frame = -1 / -2
Query: 545 VYWNSNS 525
VYWNSNS
Sbjct: 358 VYWNSNS 338
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,118,233
Number of Sequences: 8143
Number of extensions: 143009
Number of successful extensions: 1511
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 241032924
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)