TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1738959.2.2
(1454 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815139.1|CN815139 HRO4506_C09_E18ZS5 Lib AA071E1X Aven... 181 3e-054
>gb|CN815139.1|CN815139 HRO4506_C09_E18ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4506_C09_E18, mRNA sequence
Length = 476
Score = 181 bits (390), Expect(3) = 3e-054
Identities = 72/80 (90%), Positives = 79/80 (98%)
Frame = -2 / +3
Query: 550 INLGNPGEFTMLELAENVKELINPDITVTMTENTPDDPRQRKPDITKAKEVLGWEPKIVL 371
IN+GNPGEFTMLELAENVKELINP++TVTMTENTPDDPRQRKPDI+KAKE+L WEPK+VL
Sbjct: 3 INIGNPGEFTMLELAENVKELINPEVTVTMTENTPDDPRQRKPDISKAKELLDWEPKVVL 182
Query: 370 KDGLVLMEDDFRERLAVPKK 311
+DGLVLMEDDFRERLAVPKK
Sbjct: 183 RDGLVLMEDDFRERLAVPKK 242
Score = 149 bits (319), Expect(3) = 2e-042
Identities = 60/82 (73%), Positives = 66/82 (80%)
Frame = +2 / -1
Query: 305 LGFLGHGQALPEIILHEHQAVLQDDLGLPP*NFLCLGDVRLPLAGIIRSVLGHRHCYIWV 484
LG LGH QAL EI+ HEHQAV QDDLGLP L L DV LPLA +IRSVL HRHC++WV
Sbjct: 248 LGLLGHCQALSEIVFHEHQAVAQDDLGLPVQKLLGLADVGLPLARVIRSVLSHRHCHLWV 69
Query: 485 NQLLHILSKFQHGEFTWVPQVN 550
+QLLHILS+FQHGE TWVP VN
Sbjct: 68 DQLLHILSEFQHGELTWVPNVN 3
Score = 117 bits (251), Expect(3) = 1e-029
Identities = 56/75 (74%), Positives = 58/75 (77%)
Frame = -3 / +1
Query: 549 LTWGTQVNSPCWNLLRM*RS*LTQI*Q*R*PRTLLMIPARGSRTSPRQRKF*GGSPRSS* 370
LT GTQV+SPCWN LRM*RS* TQ *Q*R* RTLLM ARGS TS R R F GSPRSS
Sbjct: 4 LTLGTQVSSPCWNSLRM*RS*STQR*Q*R*LRTLLMTLARGSPTSARPRSFWTGSPRSSC 183
Query: 369 RTAWCSWRMISGSAW 325
TAWCSW+ IS SAW
Sbjct: 184 ATAWCSWKTISESAW 228
Score = 117 bits (250), Expect(3) = 3e-032
Identities = 53/80 (66%), Positives = 57/80 (71%)
Frame = +3 / -2
Query: 312 FLGTAXXXXXXXXXXXXXXXXXILGSHPRTSFALVMSGFLWRGSSGVFSVIVTVISGLIN 491
FLGTA LGS R+S AL+MSGFLWRGSSGVFSVIVTV SGLI+
Sbjct: 241 FLGTARRSLKSSSMSTKPSRRTTLGSQSRSSLALLMSGFLWRGSSGVFSVIVTVTSGLIS 62
Query: 492 SFTFSASSSMVNSPGFPKLI 551
SFTFSASSSMVNSPGFP L+
Sbjct: 61 SFTFSASSSMVNSPGFPMLM 2
Score = 96.8 bits (205), Expect = 7e-021
Identities = 46/76 (60%), Positives = 51/76 (67%)
Frame = -1 / +2
Query: 539 EPR*IHHAGTC*ECEGVD*PRYNSDDDREHS**SPPEEAGHHQGKGSSRVGAQDRPEGRL 360
EPR*+HHAGT *ECEG D PR +SDDD EHS** PEEA H QG+G+S +GAQ RP RL
Sbjct: 14 EPR*VHHAGTR*ECEGADQPRGDSDDD*EHS**PSPEEARHQQGQGASGLGAQGRPARRL 193
Query: 359 XXXXXXXXXXXGRAQE 312
G AQE
Sbjct: 194 GAHGRRFQRAPGSAQE 241
Score = 40.0 bits (81), Expect(3) = 3e-054
Identities = 18/33 (54%), Positives = 21/33 (63%)
Frame = -3 / +1
Query: 183 SSITLKDCIIRIFIQGWPFESLPTHADVVVLVF 85
SS LKDCIIR FI+GW F LP ++ L F
Sbjct: 376 SSFNLKDCIIRHFIRGWLFVVLPLLRNL*YLYF 474
Score = 32.7 bits (65), Expect(3) = 2e-042
Identities = 14/23 (60%), Positives = 16/23 (69%)
Frame = +2 / -1
Query: 116 GRDSKGQPCMKILMIQSFKVIDE 184
G + QP MK LMIQSFK+ DE
Sbjct: 443 GSTTNSQPRMKCLMIQSFKLNDE 375
Score = 29.5 bits (58), Expect(3) = 3e-032
Identities = 13/22 (59%), Positives = 16/22 (72%)
Frame = +1 / -3
Query: 115 RQRLKRPALYENSNDTILQGDR 180
R+ K+PA YE SNDTI Q +R
Sbjct: 444 RKYHKQPAPYEVSNDTIFQVER 379
Score = 27.6 bits (54), Expect(3) = 1e-029
Identities = 11/14 (78%), Positives = 11/14 (78%)
Frame = -2 / +3
Query: 172 LEGLYH*NFHTGLA 131
LE LYH* HTGLA
Sbjct: 387 LERLYH*TLHTGLA 428
Score = 25.4 bits (49), Expect(3) = 2e-042
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = +3 / -2
Query: 180 TKVYSVYWNSNS 215
TK+ VYWNSNS
Sbjct: 373 TKI*LVYWNSNS 338
Score = 25.4 bits (49), Expect(3) = 3e-032
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = +1 / -3
Query: 178 RRRYIPFIGTQIP 216
+RRY FIGTQIP
Sbjct: 375 KRRYNWFIGTQIP 337
Score = 24.9 bits (48), Expect(3) = 3e-054
Identities = 10/12 (83%), Positives = 11/12 (91%)
Frame = -1 / +2
Query: 215 GI*VPINGIYLR 180
GI*VPIN +YLR
Sbjct: 338 GI*VPINQLYLR 373
Score = 21.7 bits (41), Expect(3) = 1e-029
Identities = 6/6 (100%), Positives = 6/6 (100%)
Frame = -3 / +1
Query: 264 EHTHSW 247
EHTHSW
Sbjct: 292 EHTHSW 309
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 16,674,478
Number of Sequences: 8143
Number of extensions: 247121
Number of successful extensions: 1186
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 45
effective length of database: 1,201,052
effective search space used: 527261828
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)