TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1738931.2.11
(609 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815853.1|CN815853 HRO4511_C01_F01ZS5 Lib AA071E1X Aven... 218 8e-058
>gb|CN815853.1|CN815853 HRO4511_C01_F01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4511_C01_F01, mRNA sequence
Length = 511
Score = 218 bits (470), Expect = 8e-058
Identities = 94/123 (76%), Positives = 100/123 (81%)
Frame = +2 / +1
Query: 86 RKEEVVTREYTINLHKRLHGCTFKKKAPNAIKEIRKFAQKAMGTTDVRIDVKLNKHIWSS 265
RK+EVVTREYT+NLHKRLHGCTFKKKAPNAIKEIRKFAQKAMGTTDVRIDVKLNKHIWSS
Sbjct: 43 RKDEVVTREYTVNLHKRLHGCTFKKKAPNAIKEIRKFAQKAMGTTDVRIDVKLNKHIWSS 222
Query: 266 GIXXXXXXXXXXXXXXXNDEEDAKEELYSLVTVAEIPAEGLKGLGTKVVDETD*AVAFST 445
GI NDEEDAKEELYSLVTVAE+P EGLKGLGTKVV++ D* +
Sbjct: 223 GIRSVPRRVRVRIARKRNDEEDAKEELYSLVTVAEVPQEGLKGLGTKVVEDED*TLCRIY 402
Query: 446 SLE 454
LE
Sbjct: 403 QLE 411
Score = 119 bits (254), Expect(3) = 3e-032
Identities = 50/56 (89%), Positives = 51/56 (91%)
Frame = -1 / -3
Query: 258 QMCLLSFTSILTSVVPMAFCANFLISLMALGAFFLNVQPWRRLWRLMVYSLVTTSS 91
QMCLLSFTS+ TSVVPMAFCANFLISLMALGAFFL V PWRRLWRL VYS VTTSS
Sbjct: 215 QMCLLSFTSMRTSVVPMAFCANFLISLMALGAFFLKVHPWRRLWRLTVYSRVTTSS 48
Score = 114 bits (244), Expect = 1e-026
Identities = 47/74 (63%), Positives = 54/74 (72%)
Frame = +1 / +3
Query: 139 PRLHIQEEGTQRHQGDQEVCTEGHGHHGRQDRCEAQQAHLEQRDQERAAEGSCEDRPQEE 318
PR+++QEEG HQG+QEVCTEGHG+H RCEAQQAHLE R QE E C++ P EE
Sbjct: 96 PRMYLQEEGA*CHQGNQEVCTEGHGNHRCSHRCEAQQAHLE*RHQECPKESPCQNCP*EE 275
Query: 319 R*GGCQGGTLLPCH 360
R GGCQGG LL H
Sbjct: 276 RRGGCQGGALLSGH 317
Score = 107 bits (228), Expect = 2e-024
Identities = 49/112 (43%), Positives = 66/112 (58%)
Frame = -3 / -2
Query: 433 DSLIGFVNDLGPQPFQALGRDLRDGDKGVEFLLGILLIVPLAGDPHTNPPRHXXXXXXXD 254
+ L+ +++LG Q F+ + + D+ VE LLGILL+VPL G+ T+ P D
Sbjct: 390 EGLVLILHNLGTQTFKTFLGNFSNCDQRVELLLGILLVVPLTGNSDTDSPWDTPDAATPD 211
Query: 253 VLVELHIDPDVRGAHGLLCKLPDLLDGVGXXXXXXXXXXXXXXVDGVLPGDH 98
VLVELHID ++ G+HGLLCKLPD LDG+ +DGVL GDH
Sbjct: 210 VLVELHIDANICGSHGLLCKLPDFLDGIRRLLLEGTSVEALVEIDGVLAGDH 55
Score = 27.2 bits (53), Expect(3) = 3e-032
Identities = 9/14 (64%), Positives = 10/14 (71%)
Frame = -2 / -1
Query: 335 WHPPHRSSCGRSSH 294
WHPP RSS G+ H
Sbjct: 292 WHPPRRSSYGQF*H 251
Score = 24.4 bits (47), Expect(3) = 3e-032
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = -1 / -3
Query: 387 KPSAGISATVTRE 349
+PS G SATVTRE
Sbjct: 344 RPSWGTSATVTRE 306
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,526,516
Number of Sequences: 8143
Number of extensions: 85777
Number of successful extensions: 1531
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 196076960
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)