TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131612.2.1
(620 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN818432.1|CN818432 HRO4460_F09_K18ZS5 Lib AA070E1X Aven... 185 5e-048
gb|CN821039.1|CN821039 HRO4422_H02_O04ZS5 Lib AA069E1X Aven... 122 5e-029
>gb|CN818432.1|CN818432 HRO4460_F09_K18ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4460_F09_K18, mRNA sequence
Length = 869
Score = 185 bits (399), Expect = 5e-048
Identities = 73/91 (80%), Positives = 84/91 (92%)
Frame = +3 / +3
Query: 3 IFVCILAFMPTGWGLLLIAQAIRPVIQKIGLWGSIKALARGYEILMGLLLFTPIAFLAWF 182
IFVCILAF+PTGWG+LLIAQA RPV++ GLWGS++ALAR YE++MG+LLFTPI L+WF
Sbjct: 405 IFVCILAFLPTGWGILLIAQACRPVLRVAGLWGSVRALARAYEVIMGVLLFTPITVLSWF 584
Query: 183 PFVSEFQTRMLFNQAFSRGLQISRILGGHKK 275
PFVSEFQTRMLFNQAFSRGLQISRILGG K+
Sbjct: 585 PFVSEFQTRMLFNQAFSRGLQISRILGGQKE 677
Score = 100 bits (214), Expect = 2e-022
Identities = 49/88 (55%), Positives = 51/88 (57%)
Frame = -2 / -2
Query: 265 PPRIREICRPLLKAWLNSILVWNSDTNGNQARKAMGVNXXXXXXXXXXXXXALIDPHSPI 86
PPRIREI PLL A LNSI VWNS+T GN R MGV A DPHSP
Sbjct: 667 PPRIREI*SPLLNA*LNSIRVWNSETKGNHDRTVMGVKSSTPMITSYARARARTDPHSPA 488
Query: 85 F*ITGLIAWAIRSKPHPVGMKARMQTKM 2
TGL A AI S PHPVG ARMQT +
Sbjct: 487 TRRTGLQACAISSIPHPVGKNARMQTNI 404
>gb|CN821039.1|CN821039 HRO4422_H02_O04ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4422_H02_O04, mRNA
sequence
Length = 700
Score = 122 bits (261), Expect = 5e-029
Identities = 49/91 (53%), Positives = 62/91 (68%)
Frame = +3 / +2
Query: 3 IFVCILAFMPTGWGLLLIAQAIRPVIQKIGLWGSIKALARGYEILMGLLLFTPIAFLAWF 182
+ +C LAF+PTGWGLLLI Q +RP I+ +W I+ +A Y+ MG LLF PIA LAW
Sbjct: 104 LIICCLAFIPTGWGLLLIVQVLRPKIEYYAIWEPIQVIAHAYDYGMGSLLFFPIAILAWM 283
Query: 183 PFVSEFQTRMLFNQAFSRGLQISRILGGHKK 275
P +S QTR+LFN+AFSR LQI + G K
Sbjct: 284 PIISAIQTRVLFNRAFSRQLQIQPFIVGKTK 376
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,657,636
Number of Sequences: 8143
Number of extensions: 118415
Number of successful extensions: 2182
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 200978884
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)