TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131591.2.2
(1252 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816100.1|CN816100 HRO4517_A04_A07ZS5 Lib AA071E1X Aven... 185 2e-061
gb|CN820824.1|CN820824 HRO4420_C09_F18ZS5 Lib AA069E1X Aven... 182 3e-052
gb|CN819590.1|CN819590 HRO4401_H08_O15ZS5 Lib AA069E1X Aven... 168 1e-042
gb|CN820534.1|CN820534 HRO4413_E03_I05ZS5 Lib AA069E1X Aven... 164 4e-042
gb|CN820795.1|CN820795 HRO4414_H12_O24ZS5 Lib AA069E1X Aven... 158 2e-040
gb|CN820580.1|CN820580 HRO4415_A12_B23ZS5 Lib AA069E1X Aven... 150 5e-038
gb|CN815108.1|CN815108 HRO4505_H12_O23ZS5 Lib AA071E1X Aven... 149 6e-038
gb|CN820328.1|CN820328 HRO4418_B05_C10ZS5 Lib AA069E1X Aven... 146 9e-037
gb|CN818981.1|CN818981 HRO4474_E12_J24ZS5 Lib AA070E1X Aven... 144 2e-036
gb|CN816796.1|CN816796 HRO4524_H06_P12ZS5 Lib AA071E1X Aven... 111 2e-026
>gb|CN816100.1|CN816100 HRO4517_A04_A07ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4517_A04_A07, mRNA sequence
Length = 718
Score = 185 bits (398), Expect(2) = 2e-061
Identities = 71/97 (73%), Positives = 88/97 (90%)
Frame = +2 / +2
Query: 653 KEIVVDQVGDRATSYDDFTNSLPENDCRYAIYDFDFVTAEDVQKSRIFYILWSPSSAKVK 832
KE+V+DQVG+RA +YDDF + LPENDCRYA++DFD++T E+V KS+I+YI WSP SAKV+
Sbjct: 188 KEVVIDQVGERAATYDDFVSVLPENDCRYAVFDFDYMTKEEVPKSKIYYIFWSPDSAKVR 367
Query: 833 SKMLYASSNQKFKSGLNGIQVELQATDASEISLDEIK 943
SKMLYASSN KFK+GLNGIQ+E+QATDASEISLD I+
Sbjct: 368 SKMLYASSNSKFKTGLNGIQIEMQATDASEISLDAIQ 478
Score = 102 bits (217), Expect(2) = 1e-031
Identities = 48/97 (49%), Positives = 68/97 (70%)
Frame = -1 / -1
Query: 943 LDLIEADFTRICGLEFNLNAIEATLEFLV*ACIKHLALHLGGGWGP*DIEDPALLDILCS 764
LD +EADF I GL NL+A++A+LEF V A ++HLA HLG WGP D+ D AL ++L
Sbjct: 478 LDGVEADFAGIGGLHLNLDAVQASLEFRVRARVEHLAPHLGTVWGPEDVVDLALGNLLFG 299
Query: 763 NKVEIIDRVSAVILREAVCEVIVAGSAIPDLVHNYLL 653
+ V++ D VSAVILR E++V S +P+L+++ LL
Sbjct: 298 HVVKVKDCVSAVILR*NAYEIVVGRSTLPNLINDNLL 188
Score = 96.4 bits (204), Expect = 9e-021
Identities = 51/79 (64%), Positives = 56/79 (70%)
Frame = -2 / -2
Query: 909 VAWSST*MPLRPLLNFWFELA*SILLFTLAEDGDHRI*KILLFWTSSAVTKSKS*IAYRQ 730
VA S MP +P+LNF ELA*SILL TLA GD ++* ILL TSS V SKS AYRQ
Sbjct: 444 VACISIWMPFKPVLNFELELA*SILLLTLALSGDQKM**ILLLGTSSLVM*SKSKTAYRQ 265
Query: 729 SFSGRLFVKSS*LVARSPT 673
SFSGR KSS*+ ARSPT
Sbjct: 264 SFSGRTLTKSS*VAARSPT 208
Score = 78.0 bits (164), Expect(2) = 3e-024
Identities = 38/71 (53%), Positives = 42/71 (59%)
Frame = -3 / -3
Query: 932 RG*FHSHLWPGVQPECH*GHS*ISGLSLHKASCSSPWRRMGTIGYRRSCSSGHPLQ*QSR 753
RG*F H WP Q C S*IS S +ASCSSPW +GT RSCS PL S+
Sbjct: 467 RG*FRWHRWPASQSGCRSSQS*ISS*SSRRASCSSPWHCLGTRRCSRSCSWEPPLWSCSQ 288
Query: 752 NHRSRIGSHSQ 720
+ R RIGSHSQ
Sbjct: 287 SQRLRIGSHSQ 255
Score = 67.0 bits (140), Expect(2) = 2e-061
Identities = 24/42 (57%), Positives = 33/42 (78%)
Frame = +2 / +2
Query: 539 MANARSGVAVNDECMLKFGELQSKRLHRFITFKMDDKFKEIV 664
MANA SG VNDEC+ KF ELQ+KRLHR++T+K+ D + ++
Sbjct: 77 MANAASGAGVNDECITKFNELQAKRLHRYVTYKLQDSKEVVI 202
Score = 49.6 bits (102), Expect(2) = 1e-031
Identities = 22/36 (61%), Positives = 24/36 (66%)
Frame = -2 / -2
Query: 621 RCSLFDCSSPNFSMHSSFTATPDLAFAISGELARRK 514
RCSL CSS N MHSSFT PD AFAI+ RR+
Sbjct: 159 RCSLLACSSLNLVMHSSFTPAPDAAFAIAELGRRRR 52
Score = 49.2 bits (101), Expect(2) = 3e-024
Identities = 27/56 (48%), Positives = 34/56 (60%)
Frame = -1 / -1
Query: 700 IVAGSAIPDLVHNYLLELVVHLESYEAVQPLRLQLAKLQHALIVHSDTRSRVRHLG 533
IV G + + N L V+ L SY AVQPL LQL +L +AL++HS T RV H G
Sbjct: 238 IVVGRSTLPNLINDNLL*VLQLVSYVAVQPLGLQLVELGNALVIHSST*CRVCHCG 71
>gb|CN820824.1|CN820824 HRO4420_C09_F18ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4420_C09_F18, mRNA
sequence
Length = 610
Score = 182 bits (391), Expect(4) = 3e-052
Identities = 70/96 (72%), Positives = 87/96 (90%)
Frame = +2 / +1
Query: 659 IVVDQVGDRATSYDDFTNSLPENDCRYAIYDFDFVTAEDVQKSRIFYILWSPSSAKVKSK 838
IVVDQ+GDRA +Y+DFT LPE DCR+A+YDFD++T E+V KS+I+YI WSP +AKV+SK
Sbjct: 76 IVVDQIGDRAATYEDFTKVLPETDCRFAVYDFDYMTPEEVPKSKIYYIFWSPDTAKVRSK 255
Query: 839 MLYASSNQKFKSGLNGIQVELQATDASEISLDEIKD 946
MLYASSNQKFK+GLNGIQ+E+QATDASEISLD I++
Sbjct: 256 MLYASSNQKFKTGLNGIQIEMQATDASEISLDAIQE 363
Score = 104 bits (222), Expect = 3e-023
Identities = 45/97 (46%), Positives = 65/97 (67%)
Frame = -1 / -2
Query: 946 ILDLIEADFTRICGLEFNLNAIEATLEFLV*ACIKHLALHLGGGWGP*DIEDPALLDILC 767
+LD I+ DF I GL NL+A++A LE LV AC++HLA HL WGP D+ AL ++L
Sbjct: 363 LLDSIKTDFASISGLHLNLDAVQARLELLVRACVEHLAPHLSSVWGPEDVVYLALGNLLW 184
Query: 766 SNKVEIIDRVSAVILREAVCEVIVAGSAIPDLVHNYL 656
+ V++ID S V LR +CE+++ A+PDL+H+ L
Sbjct: 183 GHVVKVIDCKSTVSLR*HLCEILIGCCAVPDLIHDNL 73
Score = 99.1 bits (210), Expect = 1e-021
Identities = 51/78 (65%), Positives = 56/78 (71%)
Frame = -2 / -3
Query: 909 VAWSST*MPLRPLLNFWFELA*SILLFTLAEDGDHRI*KILLFWTSSAVTKSKS*IAYRQ 730
VA S MP +P+LNFW ELA*SILL TLA GD ++* ILL TSS V SKS* A RQ
Sbjct: 326 VACISIWMPFKPVLNFWLELA*SILLLTLAVSGDQKM*YILLLGTSSGVM*SKS*TANRQ 147
Query: 729 SFSGRLFVKSS*LVARSP 676
S SG FVKSS*+ ARSP
Sbjct: 146 SVSGSTFVKSS*VAARSP 93
Score = 35.4 bits (71), Expect(4) = 3e-052
Identities = 12/19 (63%), Positives = 17/19 (89%)
Frame = +2 / +3
Query: 587 KFGELQSKRLHRFITFKMD 643
KF LQ+KRLHRF+T+K++
Sbjct: 6 KFNFLQAKRLHRFVTYKLE 62
Score = 23.5 bits (45), Expect(4) = 3e-052
Identities = 6/11 (54%), Positives = 9/11 (81%)
Frame = +1 / +1
Query: 1108 KTMKVWIYHVI 1140
+ +K+W YHVI
Sbjct: 529 RPLKIWFYHVI 561
Score = 21.7 bits (41), Expect(4) = 3e-052
Identities = 7/10 (70%), Positives = 9/10 (90%)
Frame = +1 / +3
Query: 1048 KTALVICGFV 1077
+T L+ICGFV
Sbjct: 462 ETLLIICGFV 491
>gb|CN819590.1|CN819590 HRO4401_H08_O15ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4401_H08_O15, mRNA
sequence
Length = 275
Score = 168 bits (362), Expect = 1e-042
Identities = 65/88 (73%), Positives = 79/88 (89%)
Frame = +2 / +2
Query: 677 GDRATSYDDFTNSLPENDCRYAIYDFDFVTAEDVQKSRIFYILWSPSSAKVKSKMLYASS 856
G+RA +YDDF + LPENDCRYA++DFD++T E+V KS+I+YI WSP SAKV+SKMLYASS
Sbjct: 11 GERAATYDDFVSVLPENDCRYAVFDFDYMTKEEVPKSKIYYIFWSPDSAKVRSKMLYASS 190
Query: 857 NQKFKSGLNGIQVELQATDASEISLDEI 940
N KFK+GLNGIQ+E+QATDASEISLD I
Sbjct: 191 NSKFKTGLNGIQIEMQATDASEISLDAI 274
>gb|CN820534.1|CN820534 HRO4413_E03_I05ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4413_E03_I05, mRNA
sequence
Length = 566
Score = 164 bits (353), Expect(3) = 4e-042
Identities = 64/90 (71%), Positives = 79/90 (87%)
Frame = +2 / +2
Query: 677 GDRATSYDDFTNSLPENDCRYAIYDFDFVTAEDVQKSRIFYILWSPSSAKVKSKMLYASS 856
G RA +Y DFT LPE DCR+A+YDFD++T E+V KS+I+YI WSP +AKV+SKMLYASS
Sbjct: 2 GTRAATYVDFTKVLPETDCRFAVYDFDYMTPEEVPKSKIYYIFWSPDTAKVRSKMLYASS 181
Query: 857 NQKFKSGLNGIQVELQATDASEISLDEIKD 946
NQKFK+GLNGIQ+E+QATDASEISLD I++
Sbjct: 182 NQKFKTGLNGIQIEMQATDASEISLDAIQE 271
Score = 23.5 bits (45), Expect(3) = 4e-042
Identities = 6/11 (54%), Positives = 9/11 (81%)
Frame = +1 / +2
Query: 1108 KTMKVWIYHVI 1140
+ +K+W YHVI
Sbjct: 437 RPLKIWFYHVI 469
Score = 21.7 bits (41), Expect(3) = 4e-042
Identities = 7/10 (70%), Positives = 9/10 (90%)
Frame = +1 / +1
Query: 1048 KTALVICGFV 1077
+T L+ICGFV
Sbjct: 370 ETLLIICGFV 399
>gb|CN820795.1|CN820795 HRO4414_H12_O24ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4414_H12_O24, mRNA
sequence
Length = 534
Score = 158 bits (340), Expect(3) = 2e-040
Identities = 61/82 (74%), Positives = 75/82 (91%)
Frame = +2 / +3
Query: 701 DFTNSLPENDCRYAIYDFDFVTAEDVQKSRIFYILWSPSSAKVKSKMLYASSNQKFKSGL 880
DFTN LPE DCR+A+YDFD++T E+V KS+I+YI WSP +AKV+SKMLYASSNQKFK+GL
Sbjct: 6 DFTNVLPETDCRFAVYDFDYMTPEEVPKSKIYYIFWSPDTAKVRSKMLYASSNQKFKTGL 185
Query: 881 NGIQVELQATDASEISLDEIKD 946
NGIQ+E+QATDASEISLD I++
Sbjct: 186 NGIQIEMQATDASEISLDAIQE 251
Score = 23.5 bits (45), Expect(3) = 2e-040
Identities = 6/11 (54%), Positives = 9/11 (81%)
Frame = +1 / +3
Query: 1108 KTMKVWIYHVI 1140
+ +K+W YHVI
Sbjct: 417 RPLKIWFYHVI 449
Score = 21.7 bits (41), Expect(3) = 2e-040
Identities = 7/10 (70%), Positives = 9/10 (90%)
Frame = +1 / +2
Query: 1048 KTALVICGFV 1077
+T L+ICGFV
Sbjct: 350 ETLLIICGFV 379
>gb|CN820580.1|CN820580 HRO4415_A12_B23ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4415_A12_B23, mRNA
sequence
Length = 540
Score = 150 bits (322), Expect(3) = 5e-038
Identities = 58/80 (72%), Positives = 72/80 (90%)
Frame = +2 / +3
Query: 707 TNSLPENDCRYAIYDFDFVTAEDVQKSRIFYILWSPSSAKVKSKMLYASSNQKFKSGLNG 886
T LPE DCR+A+YDFD++T E+V KS+I+YI WSP +AKV+SKMLYASSNQKFK+GLNG
Sbjct: 12 TKVLPETDCRFAVYDFDYMTPEEVPKSKIYYIFWSPDTAKVRSKMLYASSNQKFKTGLNG 191
Query: 887 IQVELQATDASEISLDEIKD 946
IQ+E+QATDASEISLD I++
Sbjct: 192 IQIEMQATDASEISLDAIQE 251
Score = 23.5 bits (45), Expect(3) = 5e-038
Identities = 6/11 (54%), Positives = 9/11 (81%)
Frame = +1 / +3
Query: 1108 KTMKVWIYHVI 1140
+ +K+W YHVI
Sbjct: 417 RPLKIWFYHVI 449
Score = 21.7 bits (41), Expect(3) = 5e-038
Identities = 7/10 (70%), Positives = 9/10 (90%)
Frame = +1 / +2
Query: 1048 KTALVICGFV 1077
+T L+ICGFV
Sbjct: 350 ETLLIICGFV 379
>gb|CN815108.1|CN815108 HRO4505_H12_O23ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4505_H12_O23, mRNA sequence
Length = 500
Score = 149 bits (321), Expect(3) = 6e-038
Identities = 58/76 (76%), Positives = 70/76 (92%)
Frame = +2 / +3
Query: 716 LPENDCRYAIYDFDFVTAEDVQKSRIFYILWSPSSAKVKSKMLYASSNQKFKSGLNGIQV 895
LPENDCRYA++DFD++T E+V KS+I+YI WSP SAKV+SKMLYASSN KFK+GLNGIQ+
Sbjct: 12 LPENDCRYAVFDFDYMTKEEVPKSKIYYIFWSPDSAKVRSKMLYASSNSKFKTGLNGIQI 191
Query: 896 ELQATDASEISLDEIK 943
E+QATDASEISLD I+
Sbjct: 192 EMQATDASEISLDAIQ 239
Score = 23.5 bits (45), Expect(3) = 6e-038
Identities = 6/11 (54%), Positives = 9/11 (81%)
Frame = +1 / +3
Query: 1108 KTMKVWIYHVI 1140
+ +K+W YHVI
Sbjct: 408 RPLKIWFYHVI 440
Score = 21.7 bits (41), Expect(3) = 6e-038
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = +1 / +3
Query: 1039 VLRKTALVICGFV 1077
V +T L+ICGFV
Sbjct: 333 VTLETFLIICGFV 371
>gb|CN820328.1|CN820328 HRO4418_B05_C10ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4418_B05_C10, mRNA
sequence
Length = 540
Score = 146 bits (313), Expect(3) = 9e-037
Identities = 57/76 (75%), Positives = 69/76 (90%)
Frame = +2 / +2
Query: 716 LPENDCRYAIYDFDFVTAEDVQKSRIFYILWSPSSAKVKSKMLYASSNQKFKSGLNGIQV 895
L ENDCRYA++DFD++T E+V KS+I+YI WSP SAKV+SKMLYASSN KFK+GLNGIQ+
Sbjct: 23 LSENDCRYAVFDFDYMTKEEVPKSKIYYIFWSPDSAKVRSKMLYASSNSKFKTGLNGIQI 202
Query: 896 ELQATDASEISLDEIK 943
E+QATDASEISLD I+
Sbjct: 203 EMQATDASEISLDAIQ 250
Score = 23.5 bits (45), Expect(3) = 9e-037
Identities = 6/11 (54%), Positives = 9/11 (81%)
Frame = +1 / +2
Query: 1108 KTMKVWIYHVI 1140
+ +K+W YHVI
Sbjct: 419 RPLKIWFYHVI 451
Score = 21.7 bits (41), Expect(3) = 9e-037
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = +1 / +2
Query: 1039 VLRKTALVICGFV 1077
V +T L+ICGFV
Sbjct: 344 VTLETFLIICGFV 382
>gb|CN818981.1|CN818981 HRO4474_E12_J24ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4474_E12_J24, mRNA sequence
Length = 535
Score = 144 bits (310), Expect(3) = 2e-036
Identities = 56/74 (75%), Positives = 68/74 (91%)
Frame = +2 / +1
Query: 722 ENDCRYAIYDFDFVTAEDVQKSRIFYILWSPSSAKVKSKMLYASSNQKFKSGLNGIQVEL 901
ENDCRYA++DFD++T E+V KS+I+YI WSP SAKV+SKMLYASSN KFK+GLNGIQ+E+
Sbjct: 13 ENDCRYAVFDFDYMTKEEVPKSKIYYIFWSPDSAKVRSKMLYASSNSKFKTGLNGIQIEM 192
Query: 902 QATDASEISLDEIK 943
QATDASEISLD I+
Sbjct: 193 QATDASEISLDAIQ 234
Score = 23.5 bits (45), Expect(3) = 2e-036
Identities = 6/11 (54%), Positives = 9/11 (81%)
Frame = +1 / +1
Query: 1108 KTMKVWIYHVI 1140
+ +K+W YHVI
Sbjct: 403 RPLKIWFYHVI 435
Score = 21.7 bits (41), Expect(3) = 2e-036
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = +1 / +1
Query: 1039 VLRKTALVICGFV 1077
V +T L+ICGFV
Sbjct: 328 VTLETFLIICGFV 366
>gb|CN816796.1|CN816796 HRO4524_H06_P12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4524_H06_P12, mRNA sequence
Length = 496
Score = 111 bits (236), Expect(3) = 2e-026
Identities = 45/58 (77%), Positives = 53/58 (91%)
Frame = +2 / +3
Query: 770 EDVQKSRIFYILWSPSSAKVKSKMLYASSNQKFKSGLNGIQVELQATDASEISLDEIK 943
E+V KS+I+YI WSP SAKV+SKMLYASSN KFK+GLNGIQ+E+QATDASEISLD I+
Sbjct: 15 EEVPKSKIYYIFWSPDSAKVRSKMLYASSNSKFKTGLNGIQIEMQATDASEISLDAIQ 188
Score = 23.5 bits (45), Expect(3) = 2e-026
Identities = 6/11 (54%), Positives = 9/11 (81%)
Frame = +1 / +3
Query: 1108 KTMKVWIYHVI 1140
+ +K+W YHVI
Sbjct: 357 RPLKIWFYHVI 389
Score = 21.7 bits (41), Expect(3) = 2e-026
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = +1 / +3
Query: 1039 VLRKTALVICGFV 1077
V +T L+ICGFV
Sbjct: 282 VTLETFLIICGFV 320
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 12,277,601
Number of Sequences: 8143
Number of extensions: 182933
Number of successful extensions: 1162
Number of sequences better than 1.0e-020: 10
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 449820540
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)