TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131584.2.4
(739 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN818609.1|CN818609 HRO4467_C02_E03ZS5 Lib AA070E1X Aven... 183 3e-047
gb|CN818166.1|CN818166 HRO4471_C12_E23ZS5 Lib AA070E1X Aven... 176 3e-045
>gb|CN818609.1|CN818609 HRO4467_C02_E03ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4467_C02_E03, mRNA sequence
Length = 463
Score = 183 bits (394), Expect = 3e-047
Identities = 76/85 (89%), Positives = 80/85 (94%)
Frame = +1 / +2
Query: 268 TQVLINCRNNKKLLGRVRAFDRHCNMVLENVREMWTEIPKTGKGKKKALPVNKDRFISKM 447
TQVLINCRNNKKLLGRVRAFDRHCNMVLENVREMWTE+PKTGKGKKKALPVNKDRFISKM
Sbjct: 2 TQVLINCRNNKKLLGRVRAFDRHCNMVLENVREMWTEVPKTGKGKKKALPVNKDRFISKM 181
Query: 448 FLRGDSVIIVLRNPK*SPNQSPCSS 522
FLRGDSVIIVLRNPK* + C++
Sbjct: 182 FLRGDSVIIVLRNPK*V*RRCLCAA 256
Score = 139 bits (298), Expect = 5e-034
Identities = 63/79 (79%), Positives = 65/79 (82%)
Frame = -3 / -2
Query: 503 LGDHFGFLSTMMTESPLRNILLMNLSLFTGRAFFLPLPVLGISVHISRTFSSTMLQWRSK 324
L HFGFL TMMTESP RNILLMNLSLFTGRAFFLPLPV G SVHIS TFS TMLQ RS
Sbjct: 237 LQTHFGFLRTMMTESPRRNILLMNLSLFTGRAFFLPLPVFGTSVHISLTFSRTMLQ*RSN 58
Query: 323 ARTRPSSFLLLRQLMRTWV 267
A TRPS+FLL RQL+ T V
Sbjct: 57 ALTRPSNFLLFRQLISTCV 1
>gb|CN818166.1|CN818166 HRO4471_C12_E23ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4471_C12_E23, mRNA sequence
Length = 475
Score = 176 bits (379), Expect = 3e-045
Identities = 72/74 (97%), Positives = 74/74 (100%)
Frame = +1 / +2
Query: 277 LINCRNNKKLLGRVRAFDRHCNMVLENVREMWTEIPKTGKGKKKALPVNKDRFISKMFLR 456
LINCRNNKKLLGRVRAFDRHCNMVLENVREMWTE+PKTGKGKKKALPVNKDRFISKMFLR
Sbjct: 2 LINCRNNKKLLGRVRAFDRHCNMVLENVREMWTEVPKTGKGKKKALPVNKDRFISKMFLR 181
Query: 457 GDSVIIVLRNPK*S 498
GDSVIIVLRNPK*+
Sbjct: 182 GDSVIIVLRNPK*A 223
Score = 141 bits (302), Expect = 1e-034
Identities = 60/72 (83%), Positives = 62/72 (86%)
Frame = -3 / -2
Query: 494 HFGFLSTMMTESPLRNILLMNLSLFTGRAFFLPLPVLGISVHISRTFSSTMLQWRSKART 315
HFGFL TMMTESP RNILLMNLSLFTGRAFFLP PV G SVHIS TFS TMLQWRS A T
Sbjct: 219 HFGFLRTMMTESPRRNILLMNLSLFTGRAFFLPFPVFGTSVHISLTFSRTMLQWRSNALT 40
Query: 314 RPSSFLLLRQLM 279
RPS+FLL RQL+
Sbjct: 39 RPSNFLLFRQLI 4
Score = 97.3 bits (206), Expect = 3e-021
Identities = 41/70 (58%), Positives = 49/70 (70%)
Frame = +3 / +1
Query: 300 EAAWPCSCF*SPL*HGARERA*DVDRDPQDWQGQKEGSACEQG*IHQ*DVPQGRLGHHSA 479
E WP *SPL HG+ ER DVDR +DW+ Q+EGS+CEQG +HQ DVP RLGHH +
Sbjct: 25 EVTWPREGI*SPLQHGS*ER*GDVDRGSKDWERQEEGSSCEQGQVHQQDVPPRRLGHHCS 204
Query: 480 EEPEVIAQPV 509
EEPEV + V
Sbjct: 205 EEPEVSLRTV 234
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,628,072
Number of Sequences: 8143
Number of extensions: 122118
Number of successful extensions: 1300
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 245902276
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)