TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.662
(940 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN821190.1|CN821190 HRO4428_E05_J10ZS5 Lib AA069E1X Aven... 369 e-103
gb|CN820758.1|CN820758 HRO4414_D08_G16ZS5 Lib AA069E1X Aven... 286 4e-078
>gb|CN821190.1|CN821190 HRO4428_E05_J10ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4428_E05_J10, mRNA
sequence
Length = 811
Score = 369 bits (800), Expect = e-103
Identities = 150/173 (86%), Positives = 163/173 (94%)
Frame = -2 / +3
Query: 855 AAAMVKYSQEPGNPTKSAKAMGRDLRVHFKNTRETAFALRKLPLTKAKRYLEDVIAHKQA 676
AAAMVKYS++P NPTKSAKA G+DLRVHFKNTRETAFALRK+PL KAKRYLEDV+AHKQA
Sbjct: 90 AAAMVKYSRDPSNPTKSAKACGKDLRVHFKNTRETAFALRKMPLNKAKRYLEDVLAHKQA 269
Query: 675 IPFRRYCGGVGRTAQAKSRHSNGQGRWPVKSARFILDLLKNAESNADVKGLDVDNLYVSH 496
IPFRRYC GVGRTAQAK+R NGQGRWP KSA+F+LDLLKNAESNA+VKGLDVD+LY+SH
Sbjct: 270 IPFRRYCRGVGRTAQAKNRQPNGQGRWPAKSAQFVLDLLKNAESNAEVKGLDVDSLYISH 449
Query: 495 IQVNQAQKQRRRTYRAHGRINPYMSSPCHIELILSEKEEPVKKEADNIVAARK 337
IQVNQA KQRRRTYRAHGRINPYMS+PCHIELILSEKEEPVKKE DN+VA RK
Sbjct: 450 IQVNQAMKQRRRTYRAHGRINPYMSNPCHIELILSEKEEPVKKEPDNVVAQRK 608
Score = 312 bits (676), Expect = 5e-086
Identities = 140/173 (80%), Positives = 143/173 (82%)
Frame = +2 / -1
Query: 338 FLAATMLSASFFTGSSFSDRISSMWQGEDM*GLMRP*AR*VRRLCFWAWFTWMCET*RLS 517
FL AT LS SFFTGSSFSD+ISSMWQG DM*GLMRP*AR VRRLCF AWFTWMCE *RLS
Sbjct: 607 FLCATTLSGSFFTGSSFSDKISSMWQGLDM*GLMRP*ARYVRRLCFMAWFTWMCEM*RLS 428
Query: 518 TSKPFTSALLSAFFSKSNMNLADLTGQRPCPLEWRDFACAVRPTPPQYLRKGIACL*AIT 697
TSKP TSALLSAFFSKS+ N AD GQRP P WR FACAVRPTP QYL KGIACL A T
Sbjct: 427 TSKPLTSALLSAFFSKSSTNWADFAGQRP*PFGWRFFACAVRPTPLQYLLKGIACLWAKT 248
Query: 698 SSRYRLALVKGSLRSAKAVSLVFLKWTLRSLPMALADLVGLPGSCEYLTIAAA 856
SSRY LAL KG LR A AVS VFLKWTLRSLP ALADLVGL GS EY T+AAA
Sbjct: 247 SSRYLLALFKGILRRANAVSRVFLKWTLRSLPQALADLVGLDGSLEYFTMAAA 89
Score = 260 bits (563), Expect = 2e-070
Identities = 119/174 (68%), Positives = 137/174 (78%)
Frame = +1 / -3
Query: 340 PCRDNVVSLFLHRLFLL*QNQLNVARGGHVRVNASVSTVGAAPLLLGLVHLDV*NIEVVH 519
P D+VV L LH LFLL*Q++L+VA GHVR+NAS+STV A PLL GLVHLDV*N+E VH
Sbjct: 605 PLCDDVVRLLLHGLFLL*QDKLDVAGIGHVRINASMSTVCAPPLLHGLVHLDV*NVEAVH 426
Query: 520 VQAFHISVTLSILQQIQYEPG*LNRPATLPIGVARLCLCGATNTSTVSPEGNCLLVSNNI 699
+QAF++S+TLSILQQIQ+E G*L RPATL I +A LCLC NTS V PEGN LLV NI
Sbjct: 425 IQAFNLSITLSILQQIQHELG*LCRPATLTIWLAVLCLCCTANTSAVPPEGNSLLVGQNI 246
Query: 700 LKVSLSLGQRQLAKRKSCLPCVLEVDPQVPSHGLGRFGRVARLLRVLNHSGGEG 861
LKV LSL QR LAK K CL CVLEVDP++ + GLGR GRV R+ RVL+H GG G
Sbjct: 245 LKVPLSLVQRHLAKGKRCLACVLEVDPEILATGLGRLGRVGRIPRVLHHGGGGG 84
Score = 221 bits (478), Expect = 1e-058
Identities = 104/174 (59%), Positives = 118/174 (67%)
Frame = -1 / +2
Query: 856 RRRYG*VLAGAGQPYQIGQGHGKGPEGPLQEHKGDSFCASQAAFDQG*AIP*GCYCSQAG 677
RRR+G VL G+ QP Q+GQG +G +GPLQEH DS C SQ AF+QG* +P*GC+ QA
Sbjct: 89 RRRHGEVLEGSVQPDQVGQGLWQGSQGPLQEHTRDSVCPSQDAFEQG*EVP*GCFGPQAS 268
Query: 676 NSLPEILWRCWSHRTSKVSPLQWAGSLAC*VSQVHIGFAEEC*E*R*CERLGRGQPLCFT 497
SL E+L RCW + TSK P +W+GSLAC VS V GFAEEC*E* * RLG GQPL FT
Sbjct: 269 YSLQEVLQRCWPYSTSKEPPAKWSGSLACKVSPVRAGFAEEC*E*C*G*RLGCGQPLHFT 448
Query: 496 HPGEPSPEAEAPHLPCSRTXXXXXXXXXXX*ADSVREGRACEERG*QHCRGKEA 335
HPGEPS EAEA H+PCS T A VREGRA EE *Q R KE+
Sbjct: 449 HPGEPSHEAEAAHIPCSWTH*SLHVQSLPHRAYLVREGRAREEGA*QRRRTKES 610
Score = 196 bits (423), Expect(2) = 3e-052
Identities = 91/142 (64%), Positives = 100/142 (70%)
Frame = +3 / -2
Query: 339 SLPRQCCQPLSSQALPSLTESAQCGKGRTCKG*CVREHGRCGASASGLGSPGCVKHRGCP 518
S R+ CQ SS+ALPSLT A+CG+ TCK *CV EHG C ASAS LGSPGCVK RGCP
Sbjct: 606 SFVRRRCQAPSSRALPSLTR*ARCGRDWTCKD*CVHEHGMCAASASWLGSPGCVKCRGCP 427
Query: 519 RPSLSHQRYSQHSSANPI*TWLT*QASDPAHWSGETLLVRCDQHLHSISGRELPACEQ*H 698
PSL Q YSQHSSANP T LT QASDP H +G +LLV QHL S S RE AC H
Sbjct: 426 HPSL*PQHYSQHSSANPARTGLTLQASDPDHLAGGSLLVLYGQHLCSTS*RE*LACGPKH 247
Query: 699 PQGIA*PWSKAACEAQKLSPLC 764
PQG +*P SKA+CE Q LS +C
Sbjct: 246 PQGTS*PCSKASCEGQTLSRVC 181
Score = 188 bits (405), Expect = 1e-048
Identities = 89/137 (64%), Positives = 92/137 (67%)
Frame = -3 / +1
Query: 860 PSPPLWLSTRRSRATLPNRPRPWEGT*GSTSRTQGRQLLRFASCL*PRLSDTLRMLLLTS 681
P PP W STR R T P+RPRP GSTSRT RQ L FA CL* RL TLRM TS
Sbjct: 85 PPPPPW*STRGIRPTRPSRPRPVARISGSTSRTHARQRLPFARCL*TRLRGTLRMFWPTS 264
Query: 680 RQFPSGDTVEVLVAPHKQSLATPMGRVAGLLSQPGSYWIC*RMLRVTLM*KAWTWTTSMF 501
+ FPSG T EVL HKQ A+ M RVAGL SQP S WIC*RMLRV L KAW WT S F
Sbjct: 265 KLFPSGGTAEVLAVQHKQRTASQMVRVAGLQSQPSSCWIC*RMLRVMLRLKAWMWTASTF 444
Query: 500 HTSR*TKPRSRGAAPTV 450
HTSR*TKP SRG A TV
Sbjct: 445 HTSR*TKP*SRGGAHTV 495
Score = 24.0 bits (46), Expect(2) = 3e-052
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = +1 / -3
Query: 799 LGRFGRVARLLRVLNHSGGEGAKKE 873
LGR GR+ R+L GG ++E
Sbjct: 137 LGRVGRIPRVLHHGGGGGGAAGEEE 63
>gb|CN820758.1|CN820758 HRO4414_D08_G16ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4414_D08_G16, mRNA
sequence
Length = 610
Score = 286 bits (619), Expect = 4e-078
Identities = 116/135 (85%), Positives = 127/135 (94%)
Frame = -2 / +2
Query: 741 LRKLPLTKAKRYLEDVIAHKQAIPFRRYCGGVGRTAQAKSRHSNGQGRWPVKSARFILDL 562
LRK+PL KAKRYLEDV+AHKQAIPFRRYC GVGRTAQAK+R NGQGRWP KSA+F+LDL
Sbjct: 11 LRKMPLNKAKRYLEDVLAHKQAIPFRRYCRGVGRTAQAKNRQPNGQGRWPAKSAQFVLDL 190
Query: 561 LKNAESNADVKGLDVDNLYVSHIQVNQAQKQRRRTYRAHGRINPYMSSPCHIELILSEKE 382
LKNAESNA+VKGLDVD LY+SHIQVNQAQKQRRRTYRAHGRINPYMS+PCHIELILSEKE
Sbjct: 191 LKNAESNAEVKGLDVDALYISHIQVNQAQKQRRRTYRAHGRINPYMSNPCHIELILSEKE 370
Query: 381 EPVKKEADNIVAARK 337
EPVKKEA++ +A RK
Sbjct: 371 EPVKKEAESQIAPRK 415
Score = 229 bits (495), Expect = 5e-061
Identities = 103/138 (74%), Positives = 108/138 (78%)
Frame = +2 / -2
Query: 317 QANILHCFLAATMLSASFFTGSSFSDRISSMWQGEDM*GLMRP*AR*VRRLCFWAWFTWM 496
QA L FL A SASFFTGSSFSD++SSMW G DM*GLMRP*AR*VRR CFWAWFTWM
Sbjct: 435 QAFFL*AFLGAIWDSASFFTGSSFSDKMSSMWHGLDM*GLMRP*AR*VRRRCFWAWFTWM 256
Query: 497 CET*RLSTSKPFTSALLSAFFSKSNMNLADLTGQRPCPLEWRDFACAVRPTPPQYLRKGI 676
CE *R STS+PFTSALLSAF +S N ADL GQRP PL WR A AVRPTP QYLRKG+
Sbjct: 255 CEM*RASTSRPFTSALLSAFLRRSRTN*ADLAGQRP*PLGWRFLAWAVRPTPRQYLRKGM 76
Query: 677 ACL*AITSSRYRLALVKG 730
ACL A TSSRY LAL G
Sbjct: 75 ACLWASTSSRYLLALFSG 22
Score = 142 bits (305), Expect = 7e-035
Identities = 67/131 (51%), Positives = 95/131 (72%)
Frame = +1 / -1
Query: 358 VSLFLHRLFLL*QNQLNVARGGHVRVNASVSTVGAAPLLLGLVHLDV*NIEVVHVQAFHI 537
+SLFLH LFLL Q++L+VAR HV ++AS+STVG PLLLGLVHLDV ++E +++Q FH+
Sbjct: 394 LSLFLHWLFLLGQDELDVARVRHVGIDASMSTVGPTPLLLGLVHLDVRDVESINIQTFHL 215
Query: 538 SVTLSILQQIQYEPG*LNRPATLPIGVARLCLCGATNTSTVSPEGNCLLVSNNILKVSLS 717
S+ LS+L++IQ + L RPA+L I +A L L +NT+ V EG+ LLV +IL+V S
Sbjct: 214 SIALSVLEKIQNKLSRLGRPASLTIRLAVLSLGSTSNTTAVPTEGDGLLVGEHILQVPFS 35
Query: 718 LGQRQLAKRKS 750
L Q L + ++
Sbjct: 34 LVQWHLPEPRA 2
Score = 114 bits (243), Expect = 3e-026
Identities = 63/125 (50%), Positives = 73/125 (58%)
Frame = +3 / -3
Query: 360 QPLSSQALPSLTESAQCGKGRTCKG*CVREHGRCGASASGLGSPGCVKHRGCPRPSLSHQ 539
QPLSS ALPS T A+CG G TC+ *CV EHGR A+ASG GS GC + R P LS Q
Sbjct: 392 QPLSSLALPSRTR*ARCGTG*TCRD*CVHEHGRSDAAASGPGSLGCARCREHQHPDLSPQ 213
Query: 540 RYSQHSSANPI*TWLT*QASDPAHWSGETLLVRCDQHLHSISGRELPACEQ*HPQGIA*P 719
SQ S +P T T QAS P H +G + L + QH S GR AC + HP G *P
Sbjct: 212 HCSQRS*EDPEQTEQTWQASVPDH*AGGS*LGQYVQHHGSTYGRGWLACGRAHPPGTF*P 33
Query: 720 WSKAA 734
S A+
Sbjct: 32 CSVAS 18
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 11,473,735
Number of Sequences: 8143
Number of extensions: 172388
Number of successful extensions: 1110
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 327463922
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)