TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.654
(682 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN819385.1|CN819385 HRO4405_D04_G07ZS5 Lib AA069E1X Aven... 259 4e-072
gb|CN820486.1|CN820486 HRO4419_H07_P13ZS5 Lib AA069E1X Aven... 192 7e-050
gb|CN819555.1|CN819555 HRO4401_E07_I13ZS5 Lib AA069E1X Aven... 139 9e-037
gb|CN814880.1|CN814880 HRO4503_E04_J07ZS5 Lib AA071E1X Aven... 135 6e-033
gb|CN821066.1|CN821066 HRO4427_B06_D11ZS5 Lib AA069E1X Aven... 114 4e-029
gb|CN819715.1|CN819715 HRO4403_C08_F15ZS5 Lib AA069E1X Aven... 101 4e-025
>gb|CN819385.1|CN819385 HRO4405_D04_G07ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4405_D04_G07, mRNA
sequence
Length = 673
Score = 259 bits (560), Expect(2) = 4e-072
Identities = 104/114 (91%), Positives = 110/114 (96%)
Frame = -2 / +1
Query: 582 KDDCWLVIGGKVYNVTKFLEDHPGGDDVLLSSTAKDATDDFEDVGHSTTARAMMDEYLVG 403
+DDCWL+IGGKVYNVTKFL+DHPGGDDVLLSSTAKDATDDFEDVGHSTTARAMMDEY VG
Sbjct: 4 QDDCWLIIGGKVYNVTKFLDDHPGGDDVLLSSTAKDATDDFEDVGHSTTARAMMDEYYVG 183
Query: 402 EIDAATIPSKVKYTPPKQPHYNQDKTPEFVIKILQFLVPLAILGLAVAVRIYTK 241
EIDA TIP++VKY PKQPHYNQDKTPEF+IKILQFLVPLAILGLAVAVRIYTK
Sbjct: 184 EIDATTIPTRVKYVAPKQPHYNQDKTPEFIIKILQFLVPLAILGLAVAVRIYTK 345
Score = 227 bits (490), Expect(2) = 9e-063
Identities = 100/113 (88%), Positives = 103/113 (91%)
Frame = +2 / -3
Query: 242 LV*ILTATARPSIARGTKNWRILMTNSGVLSWL*CGCLGGVYFTLLGIVAASISPTRYSS 421
LV*I TATARPSIA+GT+NWRILM NSGVLSWL*CGCLG Y TL+GIV ASISPT YSS
Sbjct: 344 LV*IRTATARPSIAKGTRNWRILMMNSGVLSWL*CGCLGATYLTLVGIVVASISPT*YSS 165
Query: 422 IIARAVVLCPTSSKSSVASLAVEDSRTSSPPGWSSRNLVTLYTLPPMTSQQSS 580
IIARAVVL PTSSKSSVASLAVEDSRTSSPPGWSSRNLVTLYT PPM SQQSS
Sbjct: 164 IIARAVVLWPTSSKSSVASLAVEDSRTSSPPGWSSRNLVTLYTFPPMMSQQSS 6
Score = 157 bits (338), Expect(2) = 4e-052
Identities = 65/95 (68%), Positives = 69/95 (72%)
Frame = +3 / -1
Query: 297 TGGS**RTPGSYPGCSAAAWGACTSPYWVSWPHQSHRRGTRPSLHGRWCCAPHLRNHR*H 476
TGGS** T GS PGCSAAAWG T P+WVSW +SH+ TRPS GR CC PH R+ R H
Sbjct: 289 TGGS***TRGSCPGCSAAAWGQHT*PWWVSWWRRSHQHSTRPSSPGRSCCGPHPRSRRSH 110
Query: 477 PWQWKTAGHHHLQGGPPETWSHCTPCRR*RASSHP 581
PWQ KTAGH HLQGG TW HCTP RR* ASS P
Sbjct: 109 PWQLKTAGHRHLQGGHRGTW*HCTPFRR**ASSRP 5
Score = 144 bits (308), Expect(2) = 5e-042
Identities = 63/93 (67%), Positives = 67/93 (72%)
Frame = -1 / +3
Query: 574 LLARHRRQGVQCDQVSGGPPWR**CPAVFHCQGCYR*FRRCGAQHHRPCNDGRVPRR*D* 395
LLA HRR+GVQC QV PPWR* CPAVF+CQGC R R CG QH RP +DGRV *D
Sbjct: 12 LLAHHRRKGVQCHQVPR*PPWR*RCPAVFNCQGCDRRLRGCGPQHDRPGDDGRVLCW*DR 191
Query: 394 CGHDTQ*GEVHAPQAAALQPG*DPGVRHQDPPV 296
HDT G+V PQAAALQPG DP V HQDPPV
Sbjct: 192 RHHDTHQGQVCCPQAAALQPGQDPRVHHQDPPV 290
Score = 112 bits (240), Expect = 5e-026
Identities = 46/67 (68%), Positives = 57/67 (85%)
Frame = +1 / -2
Query: 379 GYRGRINLTDEVLVHHCTGGGAVPHIFEIIGSILGSGRQQDIITSRVVLQKLGHIVHLAA 558
GYRG ++LT+ VLVHH GG AV HI E++G ILGS RQQDI+TSRVV+++LG IVHL+A
Sbjct: 207 GYRGGVDLTNIVLVHHRPGGRAVAHILEVVGRILGS*RQQDIVTSRVVIEELGDIVHLSA 28
Query: 559 DDEPAVI 579
DDEPAV+
Sbjct: 27 DDEPAVV 7
Score = 98.2 bits (208), Expect(2) = 5e-022
Identities = 43/61 (70%), Positives = 45/61 (73%)
Frame = -3 / +2
Query: 581 RMTAGSSSAARCTM*PSFWRTTLEVMMSCCLPLPRMLPMISKMWGTAPPPVQ*WTSTSSV 402
R TAGSSSA RCTM PS TTLEV MSCCL LPRM P S+MW TA PP +*WTST V
Sbjct: 5 RTTAGSSSAERCTMSPSSSMTTLEVTMSCCLQLPRMRPTTSRMWATARPPGR*WTSTMLV 184
Query: 401 R 399
R
Sbjct: 185 R 187
Score = 62.0 bits (129), Expect(2) = 4e-052
Identities = 24/31 (77%), Positives = 28/31 (90%)
Frame = +1 / -2
Query: 226 LGRFRLGVDPYSNSQAQYCQGDQKLEDLDDE 318
LGR RLGVDPYS+ +AQY QGD++LEDLDDE
Sbjct: 360 LGRLRLGVDPYSDGKAQYRQGDEELEDLDDE 268
Score = 41.8 bits (85), Expect(2) = 5e-042
Identities = 16/22 (72%), Positives = 18/22 (81%)
Frame = -3 / +2
Query: 290 SPWQYWAWLLL*GSTPSRNLPS 225
SPW+YWA L GSTPSR+LPS
Sbjct: 296 SPWRYWALPSLYGSTPSRSLPS 361
Score = 28.1 bits (55), Expect(2) = 9e-063
Identities = 9/10 (90%), Positives = 9/10 (90%)
Frame = +3 / -1
Query: 225 TRQIPTWCRS 254
TRQ PTWCRS
Sbjct: 361 TRQTPTWCRS 332
Score = 27.2 bits (53), Expect(2) = 4e-072
Identities = 9/10 (90%), Positives = 10/10 (100%)
Frame = -1 / +3
Query: 253 DLHQVGICLV 224
DLHQVG+CLV
Sbjct: 333 DLHQVGVCLV 362
Score = 22.6 bits (43), Expect(2) = 5e-022
Identities = 7/10 (70%), Positives = 10/10 (100%)
Frame = -1 / +3
Query: 250 LHQVGICLVI 221
L+QVG+CLV+
Sbjct: 453 LYQVGVCLVL 482
>gb|CN820486.1|CN820486 HRO4419_H07_P13ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4419_H07_P13, mRNA
sequence
Length = 546
Score = 192 bits (413), Expect = 7e-050
Identities = 77/91 (84%), Positives = 86/91 (94%)
Frame = -2 / +2
Query: 513 GGDDVLLSSTAKDATDDFEDVGHSTTARAMMDEYLVGEIDAATIPSKVKYTPPKQPHYNQ 334
G DVL SSTAKDATDDFEDVGHSTTARAM+DEY +G++DA+TIP++ KYTPPKQPHYNQ
Sbjct: 2 GTRDVLFSSTAKDATDDFEDVGHSTTARAMLDEYYIGDVDASTIPTQTKYTPPKQPHYNQ 181
Query: 333 DKTPEFVIKILQFLVPLAILGLAVAVRIYTK 241
DKTPEF+IKILQFLVPLAILGLAVAVR+YTK
Sbjct: 182 DKTPEFIIKILQFLVPLAILGLAVAVRMYTK 274
Score = 158 bits (340), Expect = 8e-040
Identities = 72/91 (79%), Positives = 76/91 (83%)
Frame = +2 / -1
Query: 242 LV*ILTATARPSIARGTKNWRILMTNSGVLSWL*CGCLGGVYFTLLGIVAASISPTRYSS 421
LV ILTATARP +ARGT+NWRILM NSGVLSWL*CGC GGVYF +GIV AS SP YSS
Sbjct: 273 LVYILTATARPRMARGTRNWRILMMNSGVLSWL*CGCFGGVYFVWVGIVDASTSPI*YSS 94
Query: 422 IIARAVVLCPTSSKSSVASLAVEDSRTSSPP 514
IARAVVL PTSSKSSVASLAVE+ RTS P
Sbjct: 93 SIARAVVLWPTSSKSSVASLAVEEKRTSLVP 1
Score = 79.4 bits (167), Expect(2) = 2e-023
Identities = 34/63 (53%), Positives = 40/63 (63%)
Frame = +3 / -2
Query: 297 TGGS**RTPGSYPGCSAAAWGACTSPYWVSWPHQSHRRGTRPSLHGRWCCAPHLRNHR*H 476
TGGS** T GS PGCSAAA CTS VS H H+ T P+ G+ CC H ++H+ H
Sbjct: 218 TGGS***TQGSCPGCSAAASAGCTSSG*VSSTHPHHQYSTHPASRGQSCCGRHPQSHQSH 39
Query: 477 PWQ 485
PWQ
Sbjct: 38 PWQ 30
Score = 44.1 bits (90), Expect(2) = 2e-023
Identities = 20/32 (62%), Positives = 23/32 (71%)
Frame = +1 / -3
Query: 223 *LGRFRLGVDPYSNSQAQYCQGDQKLEDLDDE 318
*L R RLGV P QA+ QGD++LEDLDDE
Sbjct: 292 *LSRLRLGVHPNGYGQAKDGQGDKELEDLDDE 197
>gb|CN819555.1|CN819555 HRO4401_E07_I13ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4401_E07_I13, mRNA
sequence
Length = 504
Score = 139 bits (299), Expect(2) = 9e-037
Identities = 57/62 (91%), Positives = 59/62 (95%)
Frame = -2 / +2
Query: 426 MMDEYLVGEIDAATIPSKVKYTPPKQPHYNQDKTPEFVIKILQFLVPLAILGLAVAVRIY 247
MMDEY VGEIDA TIP+KVKY PPKQPHYNQDKTPEF+IKILQFLVPLAILGLAVAVRIY
Sbjct: 2 MMDEYYVGEIDATTIPTKVKYAPPKQPHYNQDKTPEFIIKILQFLVPLAILGLAVAVRIY 181
Query: 246 TK 241
TK
Sbjct: 182 TK 187
Score = 123 bits (264), Expect(2) = 7e-032
Identities = 53/62 (85%), Positives = 56/62 (90%)
Frame = +2 / -1
Query: 242 LV*ILTATARPSIARGTKNWRILMTNSGVLSWL*CGCLGGVYFTLLGIVAASISPTRYSS 421
LV*I TATARPSIA+GT+NWRILM NSGVLSWL*CGCLGG Y TL+GIV ASISPT YSS
Sbjct: 186 LV*IRTATARPSIAKGTRNWRILMMNSGVLSWL*CGCLGGAYLTLVGIVVASISPT*YSS 7
Query: 422 II 427
II
Sbjct: 6 II 1
Score = 75.3 bits (158), Expect(2) = 1e-027
Identities = 32/43 (74%), Positives = 34/43 (79%)
Frame = +3 / -2
Query: 297 TGGS**RTPGSYPGCSAAAWGACTSPYWVSWPHQSHRRGTRPS 425
TGGS** T GS PGCSAAAWGA T P+WVSW +S RR TRPS
Sbjct: 131 TGGS***TRGSCPGCSAAAWGART*PWWVSWWRRSRRRSTRPS 3
Score = 62.5 bits (130), Expect(2) = 1e-027
Identities = 24/31 (77%), Positives = 28/31 (90%)
Frame = +1 / -3
Query: 226 LGRFRLGVDPYSNSQAQYCQGDQKLEDLDDE 318
LGR RLGVDPY +S+AQY QGD++LEDLDDE
Sbjct: 202 LGRLRLGVDPYGDSEAQYRQGDEELEDLDDE 110
Score = 28.6 bits (56), Expect(2) = 9e-037
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = -1 / +1
Query: 253 DLHQVGICLVI*TKLARVASCT 188
DLHQVG+CLV+ + + S T
Sbjct: 175 DLHQVGVCLVLLSSVFPYGSTT 240
Score = 28.1 bits (55), Expect(2) = 7e-032
Identities = 9/10 (90%), Positives = 9/10 (90%)
Frame = +3 / -2
Query: 225 TRQIPTWCRS 254
TRQ PTWCRS
Sbjct: 203 TRQTPTWCRS 174
>gb|CN814880.1|CN814880 HRO4503_E04_J07ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4503_E04_J07, mRNA sequence
Length = 805
Score = 135 bits (290), Expect = 6e-033
Identities = 52/63 (82%), Positives = 61/63 (96%)
Frame = -2 / +1
Query: 429 AMMDEYLVGEIDAATIPSKVKYTPPKQPHYNQDKTPEFVIKILQFLVPLAILGLAVAVRI 250
AM+DEY +G++DA+TIP++ KYTPPKQPHYNQDKTPEF+IKILQFLVPLAILGLAVAVR+
Sbjct: 472 AMLDEYYIGDVDASTIPTQTKYTPPKQPHYNQDKTPEFIIKILQFLVPLAILGLAVAVRM 651
Query: 249 YTK 241
YTK
Sbjct: 652 YTK 660
Score = 113 bits (242), Expect = 3e-026
Identities = 51/77 (66%), Positives = 56/77 (72%)
Frame = +2 / -3
Query: 242 LV*ILTATARPSIARGTKNWRILMTNSGVLSWL*CGCLGGVYFTLLGIVAASISPTRYSS 421
LV ILTATARP +ARGT+NWRILM NSGVLSWL*CGC GGVYF +GIV AS SP YSS
Sbjct: 659 LVYILTATARPRMARGTRNWRILMMNSGVLSWL*CGCFGGVYFVWVGIVDASTSPI*YSS 480
Query: 422 IIARAVVLCPTSSKSSV 472
IA +S K +
Sbjct: 479 SIAEFFFFFFSSKKGMI 429
>gb|CN821066.1|CN821066 HRO4427_B06_D11ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4427_B06_D11, mRNA
sequence
Length = 420
Score = 114 bits (243), Expect(2) = 4e-029
Identities = 46/49 (93%), Positives = 48/49 (97%)
Frame = -2 / +3
Query: 387 TIPSKVKYTPPKQPHYNQDKTPEFVIKILQFLVPLAILGLAVAVRIYTK 241
TIP+KVKY PPKQPHYNQDKTPEF+IKILQFLVPLAILGLAVAVRIYTK
Sbjct: 12 TIPTKVKYAPPKQPHYNQDKTPEFIIKILQFLVPLAILGLAVAVRIYTK 158
Score = 102 bits (217), Expect(2) = 2e-025
Identities = 42/49 (85%), Positives = 45/49 (91%)
Frame = +2 / -3
Query: 242 LV*ILTATARPSIARGTKNWRILMTNSGVLSWL*CGCLGGVYFTLLGIV 388
LV*I TATARPSIA+GT+NWRILM NSGVLSWL*CGCLGG Y TL+GIV
Sbjct: 157 LV*IRTATARPSIAKGTRNWRILMMNSGVLSWL*CGCLGGAYLTLVGIV 11
Score = 62.5 bits (130), Expect(2) = 8e-022
Identities = 24/31 (77%), Positives = 28/31 (90%)
Frame = +1 / -2
Query: 226 LGRFRLGVDPYSNSQAQYCQGDQKLEDLDDE 318
LGR RLGVDPY +S+AQY QGD++LEDLDDE
Sbjct: 173 LGRLRLGVDPYGDSEAQYRQGDEELEDLDDE 81
Score = 55.6 bits (115), Expect(2) = 8e-022
Identities = 24/30 (80%), Positives = 25/30 (83%)
Frame = +3 / -1
Query: 297 TGGS**RTPGSYPGCSAAAWGACTSPYWVS 386
TGGS** T GS PGCSAAAWGA T P+WVS
Sbjct: 102 TGGS***TRGSCPGCSAAAWGART*PWWVS 13
Score = 28.6 bits (56), Expect(2) = 4e-029
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = -1 / +2
Query: 253 DLHQVGICLVI*TKLARVASCT 188
DLHQVG+CLV+ + + S T
Sbjct: 146 DLHQVGVCLVLLSSVFPYGSTT 211
Score = 28.1 bits (55), Expect(2) = 2e-025
Identities = 9/10 (90%), Positives = 9/10 (90%)
Frame = +3 / -1
Query: 225 TRQIPTWCRS 254
TRQ PTWCRS
Sbjct: 174 TRQTPTWCRS 145
>gb|CN819715.1|CN819715 HRO4403_C08_F15ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4403_C08_F15, mRNA
sequence
Length = 501
Score = 101 bits (216), Expect(2) = 4e-025
Identities = 41/46 (89%), Positives = 44/46 (95%)
Frame = -2 / +1
Query: 378 SKVKYTPPKQPHYNQDKTPEFVIKILQFLVPLAILGLAVAVRIYTK 241
++VKY PKQPHYNQDKTPEF+IKILQFLVPLAILGLAVAVRIYTK
Sbjct: 13 ARVKYVAPKQPHYNQDKTPEFIIKILQFLVPLAILGLAVAVRIYTK 150
Score = 92.7 bits (196), Expect(2) = 1e-022
Identities = 38/45 (84%), Positives = 40/45 (88%)
Frame = +2 / -2
Query: 242 LV*ILTATARPSIARGTKNWRILMTNSGVLSWL*CGCLGGVYFTL 376
LV*I TATARPSIA+GT+NWRILM NSGVLSWL*CGCLG Y TL
Sbjct: 149 LV*IRTATARPSIAKGTRNWRILMMNSGVLSWL*CGCLGATYLTL 15
Score = 28.1 bits (55), Expect(2) = 1e-022
Identities = 9/10 (90%), Positives = 9/10 (90%)
Frame = +3 / -3
Query: 225 TRQIPTWCRS 254
TRQ PTWCRS
Sbjct: 166 TRQTPTWCRS 137
Score = 27.2 bits (53), Expect(2) = 4e-025
Identities = 9/10 (90%), Positives = 10/10 (100%)
Frame = -1 / +3
Query: 253 DLHQVGICLV 224
DLHQVG+CLV
Sbjct: 138 DLHQVGVCLV 167
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,376,002
Number of Sequences: 8143
Number of extensions: 131579
Number of successful extensions: 1681
Number of sequences better than 1.0e-020: 6
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 225488504
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)